TY - GEN A1 - Hoffmann, Stephanie A1 - Schiebel, Juliane A1 - Hufert, Frank A1 - Gremmels, Heinz-Detlef A1 - Spallek, Jacob T1 - COVID-19 among Healthcare Workers: A Prospective Serological-Epidemiological Cohort Study in a Standard Care Hospital in Rural Germany T2 - International Journal of Environmental Research and Public Health N2 - Healthcare workers (HCW) play a vital role in the SARS-CoV-2 pandemic control. The aim of this study was to assess the prevalence of SARS-CoV-2 antibodies and the risk of COVID-19 infections in a cohort of HCW from four different risk groups (from intensive care unit to administration) of a hospital of a primary care level in rural Germany. The outcomes were monthly measures of antibody seroprevalence over a period of 6 months. Overall, a seroprevalence of 13.41% was determined, with significantly higher prevalence rates among HCW working in areas with more frequent contact to confirmed or suspected cases (30.30%, p = 0.003). The group specific differences in the risk of infection from COVID-19 were detected, as HCW groups with frequent exposure seemed to have an increased risk (RR = 3.18, p = 0.02; CI95 1.09–9.24). The findings contribute to the epidemiological understanding of the virus spread in an unvaccinated population group, which is highly relevant for the pandemic management. KW - SARS-CoV-2 KW - immunity KW - antibody KW - healthcare KW - staff KW - COVID-19 KW - seroprevalence KW - pandemic Y1 - 2021 UR - https://www.mdpi.com/1660-4601/18/20/10999 U6 - https://doi.org/10.3390/ijerph182010999 SN - 1660-4601 VL - 18 IS - 20 ER - TY - GEN A1 - Awan, Asad Bashir A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Sarwar, Yasra A1 - Schierack, Peter A1 - Ali, Aamir T1 - Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa T2 - EXCLI journal Y1 - 2019 SN - 1611-2156 VL - 18 SP - 79 EP - 90 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Adefioye, Olusolabomi J. A1 - Ajayeoba, T. A. A1 - Schiebel, Juliane A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria T2 - Scientific African Y1 - 2019 U6 - https://doi.org/10.1016/j.sciaf.2019.e00158 SN - 2468-2276 VL - 6 ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation T2 - Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM Y1 - 2017 PB - Spektrumverlag CY - Heidelberg ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Rödiger, Stefan A1 - Sauer, Lysann A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Weinreich, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Gerber, Ulrike A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation T2 - Frontiers in Microbiology Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01905 SN - 1664-302X VL - 9 IS - 1905 ER - TY - GEN A1 - Kramer, Toni A1 - Schiebel, Juliane A1 - Zelck, Claudia A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Genotyping and gene expression analysis of putative reference genes in Enterobacteriaceae, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, Münster Y1 - 2015 UR - https://f1000research.com/posters/5-248 U6 - https://doi.org/10.7490/f1000research.1111354.1 ER - TY - GEN A1 - Schiebel, Juliane A1 - Noack, Jonas A1 - Rödiger, Stefan A1 - Kammel, Anne A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Weise, Matthias A1 - Weiss, Romano A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Elimport, Alexey A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Analysis of three-dimensional biofilms on different material surfaces T2 - Biomaterials Science Y1 - 2020 U6 - https://doi.org/10.1039/D0BM00455C SN - 2047-4849 VL - 8 IS - 12 SP - 3500 EP - 3510 ER - TY - GEN A1 - Jurischka, Christoph A1 - Dinter, Franziska A1 - Efimova, Anastasia A1 - Weiss, Romano A1 - Schiebel, Juliane A1 - Schulz, Christian A1 - Fayziev, Bekzodjon A1 - Schierack, Peter A1 - Fischer, Thomas A1 - Rödiger, Stefan T1 - An explorative study of polymers for 3D printing of bioanalytical test systems T2 - Clinical Hemorheology and Microcirculation N2 - Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement. KW - Thermal analysis KW - 3D Printing Y1 - 2020 UR - https://pubmed.ncbi.nlm.nih.gov/31929149/ U6 - https://doi.org/10.3233/CH-190713 SN - 1875-8622 VL - 75 IS - 1 SP - 57 EP - 84 ER - TY - GEN A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Roggenbuck, Dirk A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates T2 - Applied and Environmental Microbiology Y1 - 2017 U6 - https://doi.org/10.1128/AEM.01660-17 SN - 1098-5336 SN - 0099-2240 VL - 83 IS - 24 ER -