TY - GEN A1 - Lehmann, Mario A1 - Martin, Frank A1 - Mannigel, Kerstin A1 - Kaltschmidt, Kristin A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - Three-dimensional scaffold-free fusion culture: the way to enhanced chondrogenesis of in vitro propagated human articular chondrocytes T2 - European Journal of Histochemistry KW - in vitro cartilage KW - 3-D cell culture KW - fusion culture technique KW - tissue engineering KW - cell differentiation KW - extracellular matrix KW - immunohistochemistry Y1 - 2013 U6 - https://doi.org/10.4081/ejh.2013.e31 SN - 2038-8306 SN - 1121-760X VL - 57 IS - 4 SP - 205 EP - 215 ER - TY - GEN A1 - Martin, Frank A1 - Lehmann, Mario A1 - Schläger, Peter A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - Differentiation capacity of chondrocytes in microtissues depends on TGF-ß subtyp T2 - Journal of biochips & tissue chips : JBTC KW - microtissues KW - differentiation of chondrocytes KW - TGF-β subtypes KW - BMP-7 Y1 - 2012 U6 - https://doi.org/10.4172/2153-0777.S2-002 SN - 2153-0777 N1 - Special Issue Article VL - S2 IS - 002 ER - TY - GEN A1 - Martin, Frank A1 - Lehmann, Mario A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes T2 - Experimental Biology and Medicine KW - Articular cartilage KW - 3D culture KW - microtissue KW - scaffold-free KW - personalized differentiation KW - responder/non-responder KW - personalized diagnostic platform Y1 - 2017 U6 - https://doi.org/10.1177/1535370217728498 SN - 1535-3702 VL - 242 IS - 18 SP - 1746 EP - 1756 ER - TY - CHAP A1 - Lehmann, Mario A1 - Martin, Frank A1 - Scholka, Jenny A1 - Petto, Carola A1 - Wittenburg, Gretel A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - Human chondrocytes and dental pulp stem cells enhance their chondrogenic differentiation potential via a two-step fusion culture technique T2 - Tissue Engineering, Part A, Vol. 21, Suppl. 1 KW - in vitro chondrogenesis KW - dental pulp stem cells KW - scaffold-free 3D culture KW - two-step fusion technique Y1 - 2015 U6 - https://doi.org/10.1089/ten.tea.2015.5000.abstracts SP - S-268 EP - S-269 ER - TY - GEN A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Sack, Ulrich A1 - Anderer, Ursula A1 - Conrad, Karsten T1 - Die HEp-2-Zelle als Target für multiparametrische Autoantikörperanalytik – Automatisierung und Standardisierung/The HEp-2 cell as target for multiparametric autoantibody analyses: automation and standardisation T2 - Journal of Laboratory Medicine N2 - Trotz Einführung von Multiplex-Assays ist die Bestimmung von Autoantikörpern (AAK) mittels indirekter Immunfluoreszenz (IIF) nach wie vor ein wesentlicher Bestandteil der Autoimmundiagnostik. Das Screening auf nichtorganspezifische AAK wird in der Routinediagnostik von autoimmunen rheumatischen Erkrankungen mittels indirekter Immunfluoreszenz an HEp-2-Zellen durchgeführt. Leider differieren die mit diesem Test erhaltenen Befunde z. T. recht erheblich auf Grund von objektiven (z. B. HEp-2-Zellfixierung) und subjektiven (z. B. Erfahrungswerte) Faktoren. Die Intra- und Interlaborvarianzen sind daher relativ hoch. Erste Ergebnisse von Evaluierungsstudien eines neu entwickelten Systems zur automatischen Bildanalyse (AKLIDES®) zeigen einen Weg in Richtung Standardisierung (bzw. Harmonisierung) des HEp-2-Zell-Assays auf, in dem zunächst subjektive Fehlinterpretationen vermieden werden können. Eine weitere Harmonisierung erfordert die Weiterentwicklung der Software-Algorithmen zur Mustererkennung sowie neue Kalibrationssysteme. KW - Autoantikörper KW - Automatisierung KW - HEp-2 KW - Immunfluoreszenz Y1 - 2011 U6 - https://doi.org/10.1515/JLM.2011.053 SN - 1439-0477 VL - 35 IS - 6 SP - 351 EP - 361 ER - TY - GEN A1 - Hiemann, Rico A1 - Hilger, Nadja A1 - Michel, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Anderer, Ursula A1 - Weigert, Martin A1 - Sack, Ulrich T1 - Automatic Analysis of Immunofluorescence Patterns of HEp‐2 Cells T2 - Annals of the New York Academy of Sciences N2 - The standard screening test for the recognition of autoimmune diseases is the proof of autoantibodies in serum of patients by indirect immunofluorescence (IIF) based on HEp‐2 cells. Manual evaluation of this test is very subjective, slow, and there are no objective parameters as guidelines available. Interlaboratory tests showed occasionally large deviations in the test evaluation resulting in a high variance of results. The aim of this project is fast, objective, safe, and economical automatic analysis of HEp‐2 IIF patterns. Images of IIF patterns were completely and automatically captured using an inverse motorized fluorescence microscope. Thereby, device‐specific parameters were controlled automatically, too. For fast analysis of IIF patterns new algorithms of image processing were developed. Artifacts were recognized and excluded from analysis by the developed software. Analysis of more than 80,000 images clearly demonstrated full automatization and fast processing of IIF patterns. Additionally serum‐specific fluorescence could be easily distinguished from background. Even very weak but positive patterns can be recognized and used for diagnosis. A detailed separation into different basic patterns is possible. Objective, fast, and disease‐related economical analysis of HEp‐2 immunofluorescence patterns is feasible. The implemented software algorithms allowed a mathematical way of describing IIF patterns and can therefore be a useful tool for the needed standardization process. KW - Autoimmune diagnostics KW - HEp-2 cell KW - Immunofluorescence KW - Automatic analysis KW - Screening Y1 - 2007 U6 - https://doi.org/10.1196/annals.1398.042 SN - 0077-8923 VL - 1109 IS - 1 SP - 358 EP - 371 ER - TY - GEN A1 - Willitzki, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Goihl, Alexander A1 - Hartig, Roland A1 - Conrad, Karsten A1 - Feist, Eugen A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Heiserich, Lisa A1 - Eberle, Caroline A1 - Peters, Vanessa A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk T1 - Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence T2 - Cytometry Part A Y1 - 2013 U6 - https://doi.org/10.1002/cyto.a.22350 SN - 1552-4930 SN - 1552-4922 VL - 83 IS - 11 SP - 1017 EP - 1026 ER - TY - GEN A1 - Reddig, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Hartig, Roland A1 - Heiserich, Lisa A1 - Peter, Vanessa A1 - Schierack, Peter A1 - Sack, Ulrich A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Eberle, Caroline T1 - Cytostatic drug resistance monitored by γH2AX T2 - Cytometry Part A Y1 - 2015 U6 - https://doi.org/10.1002/cyto.a.22667 SN - 1552-4930 SN - 1552-4922 VL - 87 IS - 8 SP - 724 EP - 732 ER - TY - GEN A1 - Willitzki, Annika A1 - Hiemann, Rico A1 - Peters, Vanessa A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Bogdanos, Dimitrios Petrou A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases T2 - Clinical and Developmental Immunology KW - systemic autoimmune rheumatic disease (SARD) KW - automated IIF methods KW - platform for automated interpretation of cell-based IIF testing KW - microbead immunoassays KW - multiplexing Y1 - 2012 U6 - https://doi.org/10.1155/2012/284740 SN - 1740-2530 SN - 1740-2522 SP - 1 EP - 8 ER - TY - GEN A1 - Großmann, Kai A1 - Roggenbuck, Dirk A1 - Schröder, Christian A1 - Conrad, Karsten A1 - Schierack, Peter A1 - Sack, Ulrich T1 - Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay T2 - Cytometry Part A Y1 - 2011 U6 - https://doi.org/10.1002/cyto.a.21009 SN - 1552-4922 SN - 1552-4930 VL - 79 IS - 2 SP - 118 EP - 125 ER -