TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics T2 - GeroScience Y1 - 2021 U6 - https://doi.org/10.1007/s11357-021-00443-w SN - 2509-2723 SN - 2509-2715 VL - 43 IS - 5 SP - 2249 EP - 2264 ER - TY - GEN A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Frömmel, Ulrike A1 - Weinreich, Jörg A1 - Roggenbuck, Dirk A1 - Guenther, Sebastian A1 - Schaufler, Katharina A1 - Schröder, Christian A1 - Schierack, Peter T1 - Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season T2 - Environmental Microbiology Y1 - 2015 U6 - https://doi.org/10.1111/1462-2920.12807 SN - 1462-2920 SN - 1462-2912 VL - 17 IS - 9 SP - 3352 EP - 3361 ER - TY - GEN A1 - Sauer, Lysann A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.–8.04.2016, Oxford, United Kingdom Y1 - 2016 UR - https://f1000research.com/posters/5-706 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Heiserich, Lisa A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - S. 413 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Großmann, Kai A1 - Roggenbuck, Dirk A1 - Schröder, Christian A1 - Conrad, Karsten A1 - Schierack, Peter A1 - Sack, Ulrich T1 - Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay T2 - Cytometry Part A Y1 - 2011 U6 - https://doi.org/10.1002/cyto.a.21009 SN - 1552-4922 SN - 1552-4930 VL - 79 IS - 2 SP - 118 EP - 125 ER - TY - GEN A1 - Liedtke, Victoria A1 - Rose, Laura A1 - Hiemann, Rico A1 - Nasser, Abdullah A1 - Rödiger, Stefan A1 - Bonaventura, Alena A1 - Winkler, Laura A1 - Sowa, Mandy A1 - Stöckle, Michael A1 - Schierack, Peter A1 - Junker, Kerstin A1 - Roggenbuck, Dirk T1 - Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence? T2 - International Journal of Molecular Sciences N2 - Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence. KW - LEDGF/p75 KW - autoimmunity KW - CRISPR/Cas9 KW - dsDNA KW - mDNA Y1 - 2023 U6 - https://doi.org/10.3390/ijms24076166 SN - 1422-0067 VL - 24 IS - 7 ER - TY - GEN A1 - Lopens, Steffi A1 - Wunsch, Ewa A1 - Milkiewicz, Malgorzata A1 - Röber, Nadja A1 - Zarske, Grit A1 - Nasser, Abdullah A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Rödiger, Stefan A1 - Krawczyk, Marcin A1 - Roggenbuck, Dirk A1 - Milkiewicz, Piotr T1 - PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis T2 - Diagnostics N2 - A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease. KW - primary sclerosing cholangitis KW - antibodies against serine protease proteinase-3 KW - survival KW - health-related quality of life KW - liver biochemistry KW - disease severity Y1 - 2022 U6 - https://doi.org/10.3390/diagnostics12112682 SN - 2075-4418 VL - 12 IS - 11 ER - TY - GEN A1 - Nalli, Cecilia A1 - Somma, Valentina A1 - Andreoli, Laura A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Mahler, Michael A1 - Roggenbuck, Dirk A1 - Tincani, Angela T1 - Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases T2 - Autoimmunity Highlights Y1 - 2018 U6 - https://doi.org/10.1007/s13317-018-0106-0 SN - 2038-3274 SN - 2038-0305 VL - 9 IS - 1 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Somma, Valentina A1 - Schierack, Peter A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi T1 - Autoantibody profiling in APS T2 - Lupus Y1 - 2014 U6 - https://doi.org/10.1177/0961203314534305 SN - 0961-2033 SN - 1477-0962 VL - 23 IS - 12 SP - 1262 EP - 1264 ER - TY - GEN A1 - Volkov, Ilan A1 - Seguro, Luciana A1 - Leion, Elaine P. A1 - Kovács, László A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Gilburd, Boris A1 - Doria, Andrea A1 - Tektonidou, Maria G. A1 - Agmon-Levin, Nancy T1 - Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00131-3 SN - 2038-3274 SN - 2038-0305 VL - 11 SP - 1 EP - 8 ER -