TY - GEN A1 - Roggenbuck, Johannes J. A1 - Zarske, Grit A1 - Schierack, Peter A1 - Wunderlich, Gerd A1 - Conrad, Karsten A1 - Kotzerke, Joerg A1 - Roggenbuck, Dirk A1 - Zöphel, Klaus T1 - Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) - one step less, similar results? T2 - Nuklearmedizin Y1 - 2021 U6 - https://doi.org/10.1055/a-1277-5972 SN - 0029-5566 VL - 60 IS - 1 SP - 38 EP - 46 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kolenda, Rafał A1 - Hiemann, Rico A1 - Berger, Enrico A1 - Grzymajlo, Krzysztof A1 - Swidsinski, Alexander A1 - Juretzek, Thomas A1 - Meissner, Dirk A1 - Mydlak, Karsten A1 - Reinhold, Dirk A1 - Nolan, Lisa K. A1 - Roggenbuck, Dirk T1 - Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2) T2 - Gut Y1 - 2015 U6 - https://doi.org/10.1136/gutjnl-2014-307854 SN - 1468-3288 SN - 0017-5749 VL - 64 IS - 3 SP - 517 EP - 519 ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Röber, Nadja A1 - Anderer, Ursula A1 - Csernok, Elena A1 - Bogdanos, Dimitrios Petrou A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA T2 - PLoS ONE KW - ANCA KW - vasculitis KW - screening KW - confirmatory testing KW - multiplexing KW - CytoBead technology Y1 - 2014 U6 - https://doi.org/10.1371/journal.pone.0107743 SN - 1932-6203 VL - 9 IS - 9 ER - TY - GEN A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Brumma, Therese A1 - Büttner, Thomas A1 - Großmann, Kai A1 - Cusini, Marco A1 - Pregnolato, Francesca A1 - Borghi, Maria Orietta A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk A1 - Csernok, Elena T1 - Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis T2 - Arthritis Research & Therapy KW - ANCA-associated vasculitis KW - serology KW - pattern recognition algorithms KW - discrimination and classification of cytoplasmic ANCA (C-ANCA) and perinuclear ANCA (P-ANCA) KW - automated interpretation of ANCA IIF Y1 - 2012 UR - http://arthritis-research.com/content/14/6/R271 U6 - https://doi.org/10.1186/ar4119 SN - 1465-9913 SN - 1478-6354 VL - 14 ER - TY - GEN A1 - Willitzki, Annika A1 - Hiemann, Rico A1 - Peters, Vanessa A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Bogdanos, Dimitrios Petrou A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases T2 - Clinical and Developmental Immunology KW - systemic autoimmune rheumatic disease (SARD) KW - automated IIF methods KW - platform for automated interpretation of cell-based IIF testing KW - microbead immunoassays KW - multiplexing Y1 - 2012 U6 - https://doi.org/10.1155/2012/284740 SN - 1740-2530 SN - 1740-2522 SP - 1 EP - 8 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Werner, Leal A1 - Schierack, Peter A1 - Bogdanos, Dimitrios Petrou A1 - Conrad, Karsten T1 - Glycoprotein 2 antibodies in Crohn's disease. T2 - Advances in Clinical Chemistry Y1 - 2013 SN - 0065-2423 VL - 60 SP - 187 EP - 208 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten T1 - Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2013 U6 - https://doi.org/10.1515/cclm-2013-0543 SN - 1437-4331 SN - 1434-6621 VL - 52 IS - 2 SP - e9 EP - e11 ER - TY - GEN A1 - Willitzki, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Goihl, Alexander A1 - Hartig, Roland A1 - Conrad, Karsten A1 - Feist, Eugen A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Heiserich, Lisa A1 - Eberle, Caroline A1 - Peters, Vanessa A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk T1 - Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence T2 - Cytometry Part A Y1 - 2013 U6 - https://doi.org/10.1002/cyto.a.22350 SN - 1552-4930 SN - 1552-4922 VL - 83 IS - 11 SP - 1017 EP - 1026 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Goihl, Alexander A1 - Hanack, Katja A1 - Holzlöhner, Pamela A1 - Hentschel, Christian A1 - Veiczi, Miklos A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Schulz, Hans-Ulrich T1 - Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2 T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2017 U6 - https://doi.org/10.1515/cclm-2016-0797 SN - 1437-4331 SN - 1434-6621 VL - 55 IS - 6 SP - 854 EP - 864 ER - TY - GEN A1 - Zhang, Shulan A1 - Luo, Jing A1 - Wu, Ziyan A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Li, Ji A1 - Zeng, Xiaofeng A1 - Zhang, Fengchun A1 - Qian, Jiaming A1 - Li, Yongzhe T1 - Antibodies against glycoprotein 2 display diagnostic advantages over ASCA in distinguishing CD from intestinal tuberculosis and intestinal Behçet's disease T2 - Clinical and Translational Gastroenterology Y1 - 2018 U6 - https://doi.org/10.1038/ctg.2018.1 SN - 2155-384X VL - 9 IS - 2 SP - 133 ER - TY - GEN A1 - Schlör, Anja A1 - Holzlöhner, Pamela A1 - Listek, Martin A1 - Grieß, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Füner, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk A1 - Hanack, Katja T1 - Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2 T2 - New Biotechnology Y1 - 2018 U6 - https://doi.org/10.1016/j.nbt.2018.03.006 SN - 1876-4347 SN - 1871-6784 VL - 45 SP - 60 EP - 68 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Bogdanos, Dimitrios Petrou A1 - Conrad, Karsten A1 - Laass, Martin W. T1 - Crohn's disease specific pancreatic antibodies: clinical and pathophysiological challenges T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2013 U6 - https://doi.org/10.1515/cclm-2013-0801 SN - 1437-4331 SN - 1434-6621 VL - 52 IS - 4 SP - 494 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Vermeire, Severine A1 - Hoffmann, Ilse A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Goihl, Alexander A1 - Arnim, Ulrike von A1 - De Hertogh, Gert A1 - Polymeros, Dimitrios A1 - Bogdanos, Dimitrios Petrou A1 - Bossuyt, Xavier T1 - Evidence of Crohn's disease-related anti-glycoprotein 2 antibodies in patients with celiac disease T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2015 U6 - https://doi.org/10.1515/cclm-2014-0238 SN - 1437-4331 VL - 53 IS - 9 SP - 1349 EP - 1357 ER - TY - GEN A1 - Scholz, Juliane A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Sowa, Mandy A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Bogdanos, Dimitrios Petrou A1 - Meroni, Pier Luigi A1 - Radice, Antonella A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2015 U6 - https://doi.org/10.1515/cclm-2015-0083 SN - 1437-4331 VL - 53 IS - 12 SP - 1991 EP - 2002 ER - TY - GEN A1 - Großmann, Kai A1 - Röber, Nadja A1 - Hiemann, Rico A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Simultaneous detection of celiac disease-specific IgA antibodies and total IgA T2 - Autoimmunity Highlights Y1 - 2016 U6 - https://doi.org/10.1007/s13317-016-0073-2 SN - 2038-0305 SN - 2038-3274 VL - 7 IS - 2 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Baumgart, Daniel C. A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Laass, Martin W. T1 - Autoimmunity in Crohn's Disease-A Putative Stratification Factor of the Clinical Phenotype T2 - Advances in Clinical Chemistry Y1 - 2016 U6 - https://doi.org/10.1016/bs.acc.2016.06.002 SN - 0065-2423 VL - 77 SP - 77 EP - 101 ER - TY - GEN A1 - Sowa, Mandy A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology T2 - Clinical Reviews in Allergy & Immunology Y1 - 2017 U6 - https://doi.org/10.1007/s12016-016-8574-3 SN - 1559-0267 SN - 1080-0549 VL - 53 IS - 1 SP - 87 EP - 104 ER - TY - GEN A1 - Reddig, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Hartig, Roland A1 - Heiserich, Lisa A1 - Peter, Vanessa A1 - Schierack, Peter A1 - Sack, Ulrich A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Eberle, Caroline T1 - Cytostatic drug resistance monitored by γH2AX T2 - Cytometry Part A Y1 - 2015 U6 - https://doi.org/10.1002/cyto.a.22667 SN - 1552-4930 SN - 1552-4922 VL - 87 IS - 8 SP - 724 EP - 732 ER - TY - GEN A1 - Reddig, Annika A1 - Rübe, Claudia E. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - DNA damage assessment and potential applications in laboratory diagnostics and precision medicine T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.03.06 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Sowa, Mandy A1 - Reddig, Annika A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.02 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Elmont, Emilien A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Boucraut, Joseph T1 - Autoimmune Perpheral Neuropathies and Contribution of Antiganglioside/Sulphatide Autoantibody Testing T2 - Mediterranean Jounal of Rheumatology Y1 - 2020 U6 - https://doi.org/10.31138/mjr.31.1.10 SN - 2529-198X VL - 31 IS - 1 SP - 10 EP - 18 ER - TY - GEN A1 - George, Sandra A1 - Noack, M. A1 - Vanek, Monika A1 - Rentzsch, J. A1 - Röber, Nadja A1 - Roggenbuck, Dirk A1 - Conrad, Karsten A1 - Küpper, Jan-Heiner T1 - Expression of nicotinic acetylcholine receptor subunits in HEp-2 cells for immunodetection of autoantibody specificities in sera fro Myasthenia gravis patients T2 - Clinical Hemorheology and Microcirculation Y1 - 2015 U6 - https://doi.org/10.3233/CH-151999 SN - 1875-8622 SN - 1386-0291 VL - 61 IS - 2 SP - 385 EP - 396 ER - TY - GEN A1 - George, Sandra A1 - Paulick, Silvia A1 - Knütter, Ilka A1 - Röber, Nadja A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Conrad, Karsten A1 - Küpper, Jan-Heiner T1 - Stable Expression of Human Muscle-Specific Kinase in HEp-2 M4 Cells for Automatic Immunofluorescence Diagnostics of Myasthenia Gravis T2 - PLOS one Y1 - 2014 U6 - https://doi.org/10.1371/journal.pone.0083924 SN - 1932-6203 VL - 9 IS - 1 SP - e83924 ER - TY - GEN A1 - George, Sandra A1 - Georgi, M. A1 - Roggenbuck, Dirk A1 - Conrad, Karsten A1 - Küpper, Jan-Heiner T1 - A strategy for cell-based multiplex diagnostics of Myasthenia gravis and autoimmune encephalitis by modifying the subcellular localization of cell membrane autoantigensgravis T2 - Clinical Hemorheology and Microcirculation Y1 - 2014 U6 - https://doi.org/10.3233/CH-141897 SN - 1386-0291 SN - 1875-8622 VL - 58 IS - 1 SP - 211 EP - 228 ER - TY - CHAP A1 - George, Sandra A1 - Noack, Monika A1 - Vanek, Monika A1 - Rentzsch, Juliane A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Küpper, Jan-Heiner T1 - Expression of nicotinic acetylcholine receptor subunits in HEp-2 cells for immunodetection of autoantibody specificities in sera from Myasthenia gravis patients T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - 195 EP - 196 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Bogdanos, Dimitrios Petrou A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Generalov, I. A1 - Nevinsky, G. A. A1 - Roggenbuck, Dirk T1 - Deoxyribonuclease activity of polyclonal IgGs: a putative serological marker in patients with spondyloarthritides T2 - Immunologic Research N2 - Antibodies executing catalytic activity are referred to as antibody enzymes or short “abzymes” and may have diagnostic relevance. Abzymes with deoxyribonuclease (DNase) activity have been demonstrated in patients with autoimmune and infectious diseases. Despite several reports on the occurrence of DNase abzymes in systemic autoimmune rheumatic diseases, conclusive data about DNase activity of antibodies in patients with spondyloarthritides (SpAs) are lacking. In recent cross-sectional studies evaluating levels of IgG DNase activity in patients with psoriatic arthritis (PsA), reactive arthritis (ReA), and ankylosing spondylitis (AS), DNase activity of IgG has been assessed by the rivanol clot method and confirmed by agarose gel electrophoresis. Remarkably, levels of IgG DNase activity were significantly higher in sera of SpA patients than those in control subjects. In patients with PsA, ReA, and AS, a positive correlation of DNase IgG activity with synovitis, disease activity, and stage of spondylitis was observed, respectively. Given the involvement of autoimmune reactions in cytolysis and connective tissue degradation in PsA, ReA, and to a lesser extent in AS, abzymes might have an impact on the pathophysiology of SpAs. Detection of IgG DNase activity in patients suffering from SpA represents an exciting new research field and may assist in the differential diagnosis of SpA. Y1 - 2013 U6 - https://doi.org/10.1007/s12026-013-8424-9 SN - 0257-277X SN - 1559-0755 VL - 56 IS - 2-3 SP - 457 EP - 464 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kuhl, Christoph A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Li, Ganwu A1 - Weinreich, Jörg A1 - Berger, Enrico A1 - Nolan, Lisa K. A1 - Nicholson, Bryon A1 - Römer, Antje A1 - Frömmel, Ulrike A1 - Wieler, Lothar H. A1 - Schröder, Christian T1 - Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method T2 - PLoS One N2 - We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars. Y1 - 2013 U6 - https://doi.org/10.1371/journal.pone.0059242 VL - 8 IS - 4 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Rödiger, Stefan A1 - Wex, Thomas A1 - Ansorge, Hermann A1 - Zinke, Olaf A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines T2 - Gut Pathogens N2 - Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli. Y1 - 2013 U6 - https://doi.org/10.1186/1757-4749-5-31 SN - 1757-4749 VL - 5 IS - 31 ER - TY - GEN A1 - Großmann, Kai A1 - Roggenbuck, Dirk A1 - Schröder, Christian A1 - Conrad, Karsten A1 - Schierack, Peter A1 - Sack, Ulrich T1 - Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay T2 - Cytometry Part A Y1 - 2011 U6 - https://doi.org/10.1002/cyto.a.21009 SN - 1552-4922 SN - 1552-4930 VL - 79 IS - 2 SP - 118 EP - 125 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Frömmel, Ulrike A1 - Schmidt, Carsten A1 - Ruhland, Mirko A1 - Schimke, Ingolf A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schröder, Christian ED - Seitz, Harald ED - Berger, Ingo T1 - A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies T2 - Molecular diagnostics Y1 - 2013 SN - 978-3-642-37690-0 SN - 978-3-642-37691-7 SP - 35 EP - 74 PB - Springer CY - Berlin [u.a.] ER - TY - GEN A1 - Nalli, Cecilia A1 - Somma, Valentina A1 - Andreoli, Laura A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Mahler, Michael A1 - Roggenbuck, Dirk A1 - Tincani, Angela T1 - Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases T2 - Autoimmunity Highlights Y1 - 2018 U6 - https://doi.org/10.1007/s13317-018-0106-0 SN - 2038-3274 SN - 2038-0305 VL - 9 IS - 1 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Rödiger, Stefan A1 - Sauer, Lysann A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Weinreich, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Gerber, Ulrike A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation T2 - Frontiers in Microbiology Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01905 SN - 1664-302X VL - 9 IS - 1905 ER - TY - GEN A1 - Sowa, Mandy A1 - Kolenda, Rafał A1 - Baumgart, Daniel C. A1 - Pratschke, Johann A1 - Papp, Maria A1 - Tornai, Tamas A1 - Suchanski, Jaroslaw A1 - Bogdanos, Dimitrios Petrou A1 - Mytilinaiou, Maria G. A1 - Hammermann, Jutta A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Schramm, Christoph A1 - Franke, Andre A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype T2 - Frontiers in Immunology Y1 - 2018 U6 - https://doi.org/10.3389/fimmu.2018.01959 SN - 1664-3224 VL - 9 IS - 1959 ER - TY - GEN A1 - Maslyanskiy, Aleksey A1 - Lazareva, Natalya A1 - Olinek, Polina A1 - Schierack, Peter A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Bogdanos, Dimitrios Petrou A1 - Lapin, Sergey V. A1 - Roggenbuck, Dirk T1 - Anti-hnRNP B1 (RA33) Autoantibodies Are Associated with the Clinical Phenotype in Russian Patients with Rheumatoid Arthritis and Systemic Sclerosis T2 - Journal of Immunology Research Y1 - 2014 U6 - https://doi.org/10.1155/2014/516593 VL - 2014 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Preface to the Special Issue on PCR on chip and related technologies T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1246-1 VL - 181 IS - 13/14 SP - 1609 EP - 1610 ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Hiemann, Rico T1 - Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen T2 - Journal of Laboratory Medicine Y1 - 2014 U6 - https://doi.org/10.1515/labmed-2014-0041 VL - 38 IS - 6 SP - 309 EP - 317 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Somma, Valentina A1 - Schierack, Peter A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi T1 - Autoantibody profiling in APS T2 - Lupus Y1 - 2014 U6 - https://doi.org/10.1177/0961203314534305 SN - 0961-2033 SN - 1477-0962 VL - 23 IS - 12 SP - 1262 EP - 1264 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Borghi, Maria Orietta A1 - Somma, Valentina A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Hanack, Katja A1 - Grossi, Claudia A1 - Bodio, Caterina A1 - Macor, Paolo A1 - Landenberg, Philipp von A1 - Boccellato, Francesco A1 - Mahler, Michael A1 - Meroni, Pier Luigi T1 - Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers T2 - Arthritis Research & Therapy Y1 - 2016 U6 - https://doi.org/10.1186/s13075-016-1018-x SN - 1465-9913 SN - 1478-6362 VL - 18 IS - 111 ER - TY - GEN A1 - Sowa, Mandy A1 - Trezzi, Barbara A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Somma, Valentina A1 - Sinico, Renato Alberto A1 - Roggenbuck, Dirk A1 - Radice, Antonella T1 - Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis T2 - Medicine Y1 - 2016 U6 - https://doi.org/10.1097/MD.0000000000005225 SN - 0025-7974 VL - 95 IS - 44 SP - 5225 ER - TY - GEN A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Frömmel, Ulrike A1 - Weinreich, Jörg A1 - Roggenbuck, Dirk A1 - Guenther, Sebastian A1 - Schaufler, Katharina A1 - Schröder, Christian A1 - Schierack, Peter T1 - Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season T2 - Environmental Microbiology Y1 - 2015 U6 - https://doi.org/10.1111/1462-2920.12807 SN - 1462-2920 SN - 1462-2912 VL - 17 IS - 9 SP - 3352 EP - 3361 ER - TY - GEN A1 - Villalta, Danilo A1 - Mytilinaiou, Maria G. A1 - Elsner, Martina A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Somma, Valentina A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Bogdanos, Dimitrios Petrou T1 - Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases T2 - Clinica Chimica Acta Y1 - 2015 U6 - https://doi.org/10.1016/j.cca.2015.07.021 SN - 0009-8981 VL - 450 SP - 5 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip T2 - Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 275 EP - 280 PB - Pabst Science Publ. CY - Lengerich [u.a.] ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Heiserich, Lisa A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - S. 413 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Sauer, Lysann A1 - Reddig, Annika A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, Österreich Y1 - 2016 UR - https://f1000research.com/posters/5-350 ER - TY - GEN A1 - Sauer, Lysann A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.–8.04.2016, Oxford, United Kingdom Y1 - 2016 UR - https://f1000research.com/posters/5-706 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Dinter, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Comparison of Lab and Point of Care (POC) technologies – case study for CHI3L1, Potsdam Days on Bioanalysis Y1 - 2017 UR - https://www.researchgate.net/publication/321419307 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - GEN A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Roggenbuck, Dirk A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates T2 - Applied and Environmental Microbiology Y1 - 2017 U6 - https://doi.org/10.1128/AEM.01660-17 SN - 1098-5336 SN - 0099-2240 VL - 83 IS - 24 ER - TY - GEN A1 - Liebsch, Claudia A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Fruth, Angelika A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schedler, Uwe A1 - Juretzek, Thomas A1 - Schierack, Peter T1 - Solid-phase microbead array for multiplex O-serotyping of Escherichia coli T2 - Microchimica Acta Y1 - 2017 U6 - https://doi.org/10.1007/s00604-017-2088-4 SN - 0026-3672 SN - 1436-5073 VL - 184 IS - 5 SP - 1405 EP - 1415 ER -