TY - GEN A1 - Hübener, Heike A1 - Hoffmann, Peter A1 - Keuler, Klaus A1 - Pfeifer, Susanne A1 - Ramthun, Hans A1 - Spekat, Arne A1 - Steger, Christian A1 - Warrach-Sagi, Kirsten T1 - Deriving user-informed climate information from climate model ensemble results T2 - Advances in Science and Research Y1 - 2017 U6 - https://doi.org/10.5194/asr-14-261-2017 SN - 1992-0628 SN - 1992-0636 VL - 14 SP - 261 EP - 269 ER - TY - GEN A1 - Steinbrecht, Susanne A1 - Pfeifer, Nadine A1 - Herzog, Natalie A1 - Katzenberger, Nadine A1 - Schulz, Christian A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells T2 - Toxicology Letters N2 - Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min-1 x mg-1 protein analyzed by phenacetin conversion. Both clones showed dramatically increased sensitivity to the hepatotoxic compound aflatoxin B1 (EC50<100 nM) when compared to parental HepG2 cells (EC50 ∼5 μM). Thus, newly established cell lines are an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP1A2. KW - HepG2 KW - CYP1A2 KW - Cytochrome P450 KW - Liver metabolism Y1 - 2020 U6 - https://doi.org/10.1016/j.toxlet.2019.11.006 SN - 0378-4274 VL - 319 SP - 155 EP - 159 ER - TY - GEN A1 - Bülow, Katharina A1 - Hübener, Heike A1 - Keuler, Klaus A1 - Menz, Christoph A1 - Pfeifer, Susanne A1 - Ramthun, Hans A1 - Spekat, Arne A1 - Steger, Christian A1 - Teichmann, Claas A1 - Warrach-Sagi, Kirsten T1 - User tailored results of a regional climate model ensemble to plan adaption to the changing climate in Germany T2 - Advances in Science and Research N2 - In the German regional climate modeling project ReKliEs-De the existing EURO-CORDEX simulations have been systematically complemented by new simulations to derive more robust ranges of possible future climate change. The focus of the project lay on user tailored results, which are required for the planning of measures to adapt to the changing climate. Changes in temperature and precipitation indices are calculated from a multi model ensemble for the end of the 21st century. The results for the mitigation scenario RCP2.6 are compared to the results of the “business as usual” scenario RCP8.5. Averaged over Germany the increase of mean annual temperature and of the number of summer days will be around 3 times higher for RCP8.5 than for RCP2.6. In summer, the increase of dry days could be twice as high in RCP8.5 compared to RCP2.6. Y1 - 2019 U6 - https://doi.org/10.5194/asr-16-241-2019 SN - 1992-0636 SN - 1992-0628 VL - 16 SP - 241 EP - 249 ER -