TY - GEN A1 - Lutter, Anne-Helen A1 - Liedtke, Victoria A1 - Ecke, Annemarie A1 - Scholka, Jenny A1 - Muschter, Antje A1 - Hentschel, Erik A1 - Becker, Roland A1 - Anderer, Ursula T1 - Direct co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells positively influence the differentiation potential of cartilage cells in 3D culture Y1 - 2018 U6 - https://doi.org/10.1007%2Fs10354-018-0664-9.pdf N1 - Austrian Bone Conference – ABC 2018, November 23–24, 2018, Vienna, Austria ER - TY - GEN A1 - Lutter, Anne-Helen A1 - Muschter, Antje A1 - Anderer, Ursula T1 - Diagnostic Test System to detect resorption activity of osteoclasts in vitro just using small blood samples T2 - Osteologie KW - Osteoclast KW - diagnostic Y1 - 2019 U6 - https://doi.org/10.1055/s-0039-1680038 SN - 2567-5818 VL - 28 IS - 4 PB - Georg Thieme Verlag KG CY - Stuttgart ; New York ER - TY - CHAP A1 - Lehmann, Mario A1 - Scholka, Jenny A1 - Wittenburg, Gretel A1 - Petto, Carola A1 - Geppert, Philipp A1 - Muschter, Antje A1 - Anderer, Ursula T1 - An easily accessible adult stem cell pool may be suitable for tissue engineering-based regeneration of articular cartilage defects T2 - eCM Conferences Open Access online periodical KW - adult stem cells KW - dental pulp stem cells KW - scaffold-free 3D culture KW - chondrogenic differentiation Y1 - 2016 UR - http://www.ecmjournal.org/journal/supplements/vol031supp01/vol031supp01_P.htm SN - 2522-235X N1 - TERMIS-EU Conference, Uppsala, Sweden SP - P190 ER - TY - GEN A1 - Lutter, Anne-Helen A1 - Liedtke, Victoria A1 - Scholka, Jenny A1 - Muschter, Antje A1 - Becker, Roland A1 - Anderer, Ursula T1 - Co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells or HFF-1 fibroblasts influence the differentiation potential of spheroids T2 - Journal of Cellular Biotechnology N2 - BACKGROUND: Tissue engineering has become a major field of research in biotechnology and biomedicine. As a consequence, cell-based therapeutic approaches are entering the hospitals, especially for skeletal regeneration. Traumatic injuries of cartilage are treated with autologous cell suspensions or in vitro generated cartilage tissues, but there is actually no therapy available for degenerative cartilage defects. However, Osteoarthritis (OA) is a major public health problem in the world affecting 240 million people globally. OBJECTIVE: To develop suitable in vitro tissues, the properties of chondrogenic spheroids should be optimized via coculture with cells naturally occurring as joint neighbours. METHODS: Human chondrocytes were isolated from condyles and propagated in monolayer culture. Scaffold-free spheroids were generated and co-cultured with joint-specific partner cells (osteoblast-like osteosarcoma cells, fibroblasts). Morphology and differentiation was analyzed using histochemistry (Alcian blue, Safranin O) and immunohistochemistry for cartilage markers (collagen type II, Sox9, proteoglycan), proliferation-associated protein (Ki67) and markers of connective tissue (collagen type I and actin). RESULTS: The provision of a more natural microenvironment in vitro via co-culture of chondrocyte-based aggregates with osteoblast-like Saos-2 cells enhanced the differentiation potential of chondrogenic spheroids towards hyaline cartilage. CONCLUSIONS: The study showed the positive influence of Saos-2 cells on the differentiation potential of human chondrocytes in co-culture. Y1 - 2018 U6 - https://doi.org/10.3233/JCB-189005 SN - 2352-3697 SN - 2352-3689 VL - 4 IS - 1/2 SP - 31 EP - 42 ER -