TY - GEN A1 - Mohsin, Mashkoor A1 - Raza, Shahbaz A1 - Schaufler, Katharina A1 - Roschanski, Nicole A1 - Sarwar, Fatima A1 - Semmler, Torsten A1 - Schierack, Peter A1 - Guenther, Sebastian T1 - High Prevalence of CTX-M-15-Type ESBL-Producing E. coli from Migratory Avian Species in Pakistan T2 - Frontiers in microbiology Y1 - 2017 U6 - https://doi.org/10.3389/fmicb.2017.02476 SN - 1664-302X VL - 8 IS - 2476 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Wieler, Lothar H. T1 - Probiotic Escherichia coli Nissle 1917 reduces growth, Shiga toxin expression, release and thus cytotoxicity of enterohemorrhagic Escherichia coli T2 - International Journal of Medical Microbiology Y1 - 2015 U6 - https://doi.org/10.1016/j.ijmm.2014.10.003 SN - 1618-0607 SN - 1438-4221 VL - 305 IS - 1 SP - 20 EP - 26 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Raza, Shahbaz A1 - Roschanski, Nicole A1 - Guenther, Sebastian A1 - Ali, Aamir A1 - Schierack, Peter T1 - Description of the First Escherichia coli Clinical Isolate Harboring the Colistin Resistance Gene mcr-1 from the Indian Subcontinent T2 - Antimicrobial Agents and Chemotherapy Y1 - 2016 U6 - https://doi.org/10.1128/AAC.01945-16 SN - 1098-6596 SN - 0066-4804 VL - 61 IS - 1 ER - TY - GEN A1 - Umair, Muhammad A1 - Mohsin, Mashkoor A1 - Ali, Qasim A1 - Qamar, Muhammad U. A1 - Raza, Shahbaz A1 - Ali, Aamir A1 - Guenther, Sebastian A1 - Schierack, Peter T1 - Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan T2 - Microbial drug resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0450 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 9 SP - 1374 EP - 1381 ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Mushtaq, Muhammad Ahmed A1 - Abbas, Nayyar A1 - Fatima, Fariha A1 - Gibbon, Marjorie J. A1 - Schierack, Peter A1 - Mohsin, Mashkoor T1 - Occurrence, antimicrobial resistance and genomic features of Klebsiella pneumoniae from broiler chicken in Faisalabad, Pakistan T2 - Frontiers in Veterinary Science N2 - IntroductionThe dissemination of antimicrobial resistance (AMR) in critical priority pathogens is a significant threat. Non-clinical reservoirs of AMR, such as agriculture and food production facilities, may contribute to the transmission of clinically relevant pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. There is currently very limited knowledge regarding the population structure and genomic diversity of K. pneumoniae in poultry production in Pakistan.MethodsWe explored healthy broilers in a commercial farm from Faisalabad, Pakistan, and identified six K. pneumoniae strains from 100 broiler birds. We characterized the strains, determining clonality, virulence and antimicrobial resistance genes using next generation sequencing.ResultsThe evaluation of antimicrobial susceptibility revealed that all the strains were MDR. Genomic analysis showed that 3/6 strains belonged to ST152, harbouring acquired resistance aminoglycosides [aadA2, aph(4′)-Ia], β-lactams (blaSHV-187, blaLAP2), fosfomycin (fosA6), tetracycline (tetA), trimethoprim (dfrA12), quinolone (qnrS1), sulphonamides (sul2) and phenicol (floR). All the strains harboured the efflux pump genes oqxA, oqxB, emrR, kpnG, kpnH, kpnF, baeR, mtdB and mtdC. All six strains encoded identical virulence profiles possessing six genes, i.e., ureA, iutA, entB, allS, fimH and mrkD. Phylogenomic analysis of the dominant sequence type (ST152) present in our dataset with publicly available genomes showed that the isolates clustered to strains mainly from human sources and could pose a potential threat to food safety and public health.DiscussionThe combination of these findings with antimicrobial use data would allow a better understanding of the selective pressures that may be driving the spread of AMR. This is the first report of MDR K. pneumoniae isolated from broiler hens in Pakistan, and the finding suggests that routine surveillance of WHO critical priority pathogens in such settings would be beneficial to the development of effective control strategies to reduce AMR. KW - antimicrobial resistance KW - poultry KW - genomics KW - sequence type Y1 - 2024 U6 - https://doi.org/10.3389/fvets.2024.1433124 SN - 2297-1769 VL - 11 PB - Frontiers Media S.A. ER -