TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive sensors based on photografted molecularly imprinted polymers T2 - Biocybernetics and Biomedical Engineering N2 - The reversible chemosensors to creatinine and desmetryn based on artificial chemoreceptors are described. A grafted photopolymerization combined with a technique of molecular imprinting was used for the receptor layer preparation. After polymer deposition and intensive washing from templates, the electrodes were investigated by impedance spectrometry. Creatinine and desmetryn binding were detected as a decrease in the electrode capacitance. Sensor response to creatining was highly selective. No response to the addition of sodium chloride, creatine, urea or glucose were observed. The electrodes coated with the molecularly imprinted polymers selective to desmetryn displayed specific binding of this herbicide: only small capacitive effects were observed to addition of terbumeton or atrazine, while metribuzine displayed capacitance decrease similar to desmetryn. KW - capacitive sensor KW - creatinine KW - atrazine KW - chemical sensor KW - photografted polymerization Y1 - 2001 SN - 0208-5216 VL - 21 IS - 3 SP - 43 EP - 54 ER - TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Ulbricht, Mathias A1 - Wolfbeis, Otto S. T1 - Impedometric herbicide chemosensors based on molecularly imprinted polymers T2 - Analytica Chimica Acta N2 - The technique of grafting polymerization has been used for preparation of thin films of molecularly imprinting polymers on the surface of polypropylene membranes and on hydrophobized gold electrodes. The herbicide desmetryn was used as a template. The solid supports used were hydrophobic, while the polymer was hydrophilic. The adsorbed layer of benzophenone, irradiated by UV-light, initiated a radical polymerization near the surface. Polymer films were characterized by weighing, contact angle measurements and impedance spectroscopy. The electrodes coated with the molecularly imprinted polymers displayed fairly specific binding of desmetryn, as detected by the decrease in the capacitance of the electrode. Only small capacitive effects were observed on addition of terbumeton or atrazine, while metribuzine displayed capacitance decrease similar to desmetryn. KW - Molecular imprinting KW - Capacitive sensor KW - Chemical sensor KW - Grafting polymerization KW - Photopolymerization Y1 - 2001 U6 - https://doi.org/10.1016/S0003-2670(00)01280-0 SN - 0003-2670 VL - 435 IS - 1 SP - 157 EP - 162 ER - TY - GEN A1 - Vasjari, Majlinda A1 - Mirsky, Vladimir M. T1 - Calibrated nanoinjections of mercury vapor T2 - Fresenius' Journal of Analytical Chemistry N2 - A simple technique for the calibration of mercury vapor chemosensors is described. It is based on a reductive deposition of a well-defined quantity of mercury (0) onto gold wire followed by thermal evaporation of the mercury into the sensor cell. The quantity of mercury on the gold wire was measured electrochemically by oxidation before and after heating, as well as after storage under different conditions. It is shown that the technique can be used for calibrated injections of nanogram quantities of mercury. The approach was applied to test the performance of ultrasensitive mercury chemoresistors. Y1 - 2000 SN - 1618-2642 SN - 1618-2650 VL - 368 IS - 7 SP - 727 EP - 729 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Wrobel, Nadia A1 - Riepl, Michael A1 - Hirsch, Thomas A1 - Wolfbeis, Otto S. T1 - Intelligente Nanostrukturen für Biosensoren und Sensor-Arrays T2 - BioTec: Zeitschrift für Biotechnologie Y1 - 1999 SN - 0937-2725 VL - 10 SP - 28 EP - 32 ER - TY - CHAP A1 - Wrobel, Nadia A1 - Riepl, Michael A1 - Schinkinger, Manfred A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive thin film biosensors for bacteriophages and nucleic acids T2 - Proceedings, EMBEC ’99, November 4 - 7, 1999, Vienna, Austria Y1 - 1999 SP - 362 EP - 363 PB - IFMBE CY - Stevenage ER - TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Piletsky, Sergey A. A1 - Wolfbeis, Otto S. T1 - Electropolymerized molecularly imprinted polymers as receptor layers in capacitive chemical sensors T2 - Analytical Chemistry N2 - The first application of molecularly imprinted polymers to chemical sensors with capacitive detection is described. The sensitive layer was prepared by electropolymerization of phenol on gold electrodes in the presence of the template (phenylalanine). The insulating properties of the polymer layer were studied by electrochemical impedance spectroscopy. Electrical leakages through the polymer layer were suppressed by deposition of a self-assembled monolayer of mercaptophenol before polymerization and of alkanethiol after polymerization. At the final stage of sensor preparation, the template was removed. The multilayer system obtained displayed a decrease in electrical capacitance on addition of phenylalanine. Only a low response was observed toward other amino acids and phenol. Y1 - 1999 U6 - https://doi.org/10.1021/ac9903196 SN - 0003-2700 SN - 1520-6882 VL - 71 IS - 20 SP - 4609 EP - 4613 ER - TY - GEN A1 - Hirsch, Thomas A1 - Kettenberger, Hubert A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - A simple strategy for preparation of sensor arrays: Nanostructured monolayers as recognition elements T2 - Chemical Communications N2 - The spreader-bar approach is a simple method for producing a huge variety of receptors with different selectivities. A sensor-array consisting of five such receptors is presented. A pattern recognition provides selective detection of different purines and pyrimidines. Y1 - 2003 U6 - https://doi.org/10.1039/b210554c SN - 1359-7345 SN - 1364-548X IS - 3 SP - 432 EP - 433 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Hirsch, Thomas A1 - Panasyuk, Tatiana L. A1 - Piletsky, Sergey A. A1 - Wolfbeis, Otto S. T1 - Two- and three-dimensional artificial chemoreceptors: molecular spreader-bar vs. molecular imprinting T2 - Medical and Biological Engineering and Computing Y1 - 1999 SN - 0140-0118 SN - 1741-0444 VL - 37 IS - suppl.2 SP - 344 EP - 345 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Hirsch, Thomas A1 - Piletsky, Sergey A. A1 - Wolfbeis, Otto S. T1 - A spreader-bar approach to molecular architecture: formation of stable artificial chemoreceptors T2 - Angewandte Chemie International Edition N2 - The destructive influence of lateral diffusion on nanostructured monolayers can be prevented by using the spreader-bar technique. This approach allows the formation of stable artificial receptors for barbituric acid by lateral structuring of a dodecanethiol monolayer with molecular spreader-bars from thiobarbituric acid without chemical polymerization (see schematic representation). The new technique may have applications in chemosensors, affinity chromatography, stereoselective catalysis, and molecular electronics. KW - Molecular architecture KW - Molecular recognition KW - Monolayers KW - Nanostructures KW - Sensors Y1 - 1999 U6 - https://doi.org/10.1002/(SICI)1521-3773(19990419)38:8<1108::AID-ANIE1108>3.0.CO;2-C SN - 1521-3773 VL - 38 IS - 8 SP - 1108 EP - 1110 ER - TY - GEN A1 - Riepl, Michael A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Electrical control of alkanethiols self-assembly on a gold surface as an approach for preparation of microelectrode arrays T2 - Microchimica Acta N2 - It is shown by capacitive monitoring that the self-assembly of alkanethiols on gold electrodes and desorption of these self-assembled monolayers from the electrodes are controlled by the electrode potential. At neutral pH, chemical adsorption of alkanethiols was observed at an electrode potential of +300 mV vs SCE, but only physical adsorption was detected when the electrode potential was −1400 mV vs SCE. At electrode potentials between these values (−300 mV, −600 mV), chemical adsorption of alkanethiols occurred, but the alkanethiol monolayers were not stable in the absence of the alkanethiol in the bulk solution and were desorbed from the gold electrode. The desorption rate was higher at more negative electrode potentials. These results can be used in designing methods for electrically addressable immobilization of different receptors on (micro)electrode arrays. This has been demonstrated by deposition of two different types of alkanethiols onto a two-electrode array. KW - self-assembly KW - electrical control KW - electrode capacitance KW - addressable immobilization KW - (bio)sensor array Y1 - 1999 U6 - https://doi.org/10.1007/s006040050006 SN - 0026-3672 SN - 1436-5073 VL - 131 IS - 1 SP - 29 EP - 34 ER - TY - GEN A1 - Schweiß, Rüdiger A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive study of self-assembled alkylthiol monolayers: surface charge effects and kinetics of surfactants adsorption T2 - Materials Science Forum Y1 - 1998 U6 - https://doi.org/10.4028/www.scientific.net/MSF.287-288.427 SN - 1662-9752 SN - 0255-5476 VL - 287-288 SP - 427 EP - 430 ER - TY - GEN A1 - Ando, Masanori A1 - Swart, Claudia A1 - Pringsheim, Erika A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Optical ozone detection by use of polyaniline film T2 - Solid State Ionics N2 - We report on the optical ozone sensitivity of a polyaniline (PANI) film prepared on the inner wall of a polystyrene cuvette by chemical oxidation of aniline from aqueous solution. The PANI film showed changes of optical absorbance in the presence of 50–100 ppm ozone in air at room temperature. The absorbance change was largest at wavelengths in the range of 500–800 nm. The absorbance changes brought about by ozone was partially reversible after removal of ozone from the atmosphere. These results suggest that PANI is a potential material for optical ozone detection. KW - Optical ozone detection KW - Polyaniline film KW - Visible near-IR absorption Y1 - 2002 U6 - https://doi.org/10.1016/S0167-2738(02)00338-7 SN - 0167-2738 VL - 152-153 SP - 819 EP - 822 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - New applications of self-assembled monolayers (Invited review) T2 - Trends in Analytical Chemistry N2 - New applications of self-assembled monolayers of thiol compounds on gold electrodes are reviewed. They include: (i) exploitation of electrical control of self-assembly of thiol compounds for electrically- addressable immobilization of receptor molecules onto sensor arrays; (ii) a spreader-bar technique for formation of stable nanostructures; and, (iii) use of self-assembled monolayers as selective filters for chemical sensors. Y1 - 2002 U6 - https://doi.org/10.1016/S0165-9936(02)00601-5 SN - 0165-9936 VL - 21 IS - 6-7 SP - 439 EP - 450 ER - TY - GEN A1 - Wrobel, Nadia A1 - Schinkinger, Manfred A1 - Mirsky, Vladimir M. T1 - A novel ultraviolet assay for testing side reactions of carbodiimides T2 - Analytical Biochemistry N2 - Carbodiimides possess considerable absorbance in the ultraviolet region; the extinction coefficient of 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC) in water is ϵ214 = 6.3 · 103 L mol−1 cm−1. It provides a very simple method for testing possible side reactions of carbodiimides. This technique was used to study effects of pH, different buffers, and other components typically present in biological samples on EDC stability. It was shown that the hydrolysis rate in pure water increased from 1.5 · 10−5 to 5 · 10−4 s−1 by pH decreasing from 7 to 4. A strong increase of the rate of EDC loss was observed in the presence of different components. This effect can be described by the following row of hydrolysis rates: citrate ⪢ acetate ≈ phosphate ⪢ SDS. The results can be used to optimize carbodiimide-mediated reactions of peptide bond formation in organic chemistry or peptide synthesis or during immobilization or cross-linking of biological molecules. Y1 - 2002 U6 - https://doi.org/10.1006/abio.2002.5646 SN - 0003-2697 VL - 305 IS - 2 SP - 135 EP - 138 ER - TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive creatinine sensor based on molecularly imprinted polymer T2 - Electroanalysis N2 - The first reversible chemosensor to creatinine based on artificial chemoreceptors is described. The receptor layer was prepared by molecularly imprinted photopolymerization of acrylamidomethylpropanesulfonic acid and methylenediacrylamide. The polymer layer was grafted onto the surface of gold electrodes coated with an alkanethiol monolayer. Creatinine binding was detected by a decrease in the electrode capacitance. The sensor response is reversible and highly selective: no response to addition of sodium chloride, creatine, urea or glucose were observed. The detection limit for creatinine is 10 μM which is optimal for medical applications. KW - Capacitive sensor KW - Creatinine KW - Molecularly imprinted polymer KW - Chemical sensor KW - Photografted polymerization Y1 - 2002 U6 - https://doi.org/10.1002/1521-4109(200202)14:3<221::AID-ELAN221>3.0.CO;2-Y SN - 1521-4109 VL - 14 IS - 3 SP - 221 EP - 224 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - Affinity sensors in non-equilibrium conditions: a highly selective chemosensing by means of low selective chemosensors T2 - Sensors N2 - The selectivity of measurements by means of affinity sensors in (quasi)equilibrium conditions and in non-equilibrium conditions was compared. The results show that the measurements in non-equilibrium conditions can reduce or even eliminate a relative contribution of interferents to a sensor signal. KW - affinity sensors KW - kinetics KW - selectivity Y1 - 2001 U6 - https://doi.org/10.3390/s10100013 SN - 1424-8220 VL - 1 IS - 1 SP - 13 EP - 17 ER - TY - CHAP A1 - Wu, Meng A1 - Lin, Zhihong A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. ED - Zhou, Yikai T1 - Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA T2 - Proceedings, International Conference on Sensor Technology (ISTC 2001), 10 - 12 October 2001, Wuhan, China N2 - In this research, a surface-plasmon resonance biosensor for nucleic acid detection was constructed, optimized and characterized. The kinetic response on hybridization was also studied. The biosensor was use as a platform for the study of the surface coverage control by single-strand or double-strand oligonucleotide immobilization. The dsDNA immobilization and offers a new way to surface coverage control for the nucleic acid biosensor construction. Y1 - 2001 SN - 0-8194-4119-8 U6 - https://doi.org/10.1117/12.440180 SP - 23 EP - 26 PB - SPIE CY - Bellingham, Wash. ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Panasyuk-Delaney, Tanya A1 - Wolfbeis, Otto S. T1 - Chemical sensors based on ultrathin membranes from molecularly imprinted polymers T2 - Membrane & cell biology Y1 - 2001 VL - 18 IS - 6 SP - 496 EP - 501 ER - TY - CHAP A1 - Lin, Zhihong A1 - Wu, Meng A1 - Ren, Shu A1 - Arbter, Michaela A1 - Boehmer, Martin A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. ED - Zhou, Yikai T1 - Study of single and dual NIR fluorescently labeled nucleic acid conjugates for nucleic acid detection T2 - Proceedings, International Conference on Sensor Technology (ISTC 2001), 10 - 12 October 2001, Wuhan, China N2 - In this research, a near-IR fluorescent labeled nucleic acid conjugate for the nucleic acid detection was synthesized, and characterized preliminarily for the detection of the nucleic acid. Th conjugate combines the molecular recognition properties of the oligonucleotides with the near-IR fluorescence label PR646. Both single- and dual- labeled conjugates were studied for their hybridization with the complementary nucleic acid. The dual labeled conjugate has indicated that the self-quenching effect exists in ssDNA form while the fluorescence increases greatly after hybridization with the complementary nucleic acids. The time-resolved fluorescence was also studied. KW - Near infrared KW - luminescence Y1 - 2001 SN - 0-8194-4119-8 U6 - https://doi.org/10.1117/12.440165 SP - 111 EP - 114 PB - SPIE CY - Bellingham, Wash. ER - TY - CHAP A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Mass, Markus A1 - Hirsch, T. A1 - Schweiss, R. A1 - Wolfbeis, Otto S. ED - Vincenzini, Pietro ED - Dori, L. T1 - Capacitive detection of analyte binding in thin film chemo- and biosensors T2 - Solid State Chemical and Biochemical Sensors Y1 - 1999 SN - 88-86538-27-8 SP - 441 EP - 448 PB - Techna CY - Faenza ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Mass, Markus A1 - Krause, Christian A1 - Wolfbeis, Otto S. T1 - Capacitive approach to determine phospholipase A2 activity towards artificial and natural substrates T2 - Analytical Chemistry N2 - A capacitive approach has been employed to develop a novel method to determine phospholipase activity. The sensing electrodes have a structure like Au/S(CH₂)₁₇CH₃/substrate/electrolyte. Hydrolysis of the substrate, mediated by phospholipase A₂, leads to the formation of water-soluble products from the insoluble substrate. This results in desorption of these products into aqueous phase and corresponding increase of the electrode capacitance. The requirement of high water solubility of the reaction products can be achieved in two ways. In the first, short-chain phospholipids are used as the substrate, in which case, water-soluble products are formed and no additional reagents are required to promote desorption of these products. The sensors prepared by this strategy provide sensitive qualitative detection of phospholipases. The second way is based on the use of a water-soluble acceptor (for example, β-cyclodextrin) to solubilize the products of hydrolysis. It allows semiquantitative detection of phospholipase activity toward long-chain natural substrates. The reaction kinetics for this case was found to be monoexponential and linearly dependent on the phospholipase concentration. The detection limit of this method, as tested with phospholipase A₂ from bee venom and soy bean lecithin as the substrate, is ∼0.5 ng/mL (500 μunits/mL). Y1 - 1998 U6 - https://doi.org/10.1021/ac980102w SN - 0003-2700 SN - 1520-6882 VL - 70 IS - 17 SP - 3674 EP - 3678 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Wolfbeis, Otto S. T1 - Capacitive monitoring of protein immobilization and antigen-antibody reaction on the monomolecular films of alkylthiols adsorbed on gold electrodes T2 - Biosensors and Bioelectronics N2 - Self-assembled monolayers of omega-mercaptohexadecanoic acid and omega-mercaptohexadecylamine on gold electrodes are stable at neutral pH and display pure capacitive behavior at frequencies around 20 Hz. Different methods of covalent immobilization of proteins on these monolayers are compared. Various reagents including succinimides, thionylchloride, p-nitrophenol and carbodiimides were used to activate the carboxy groups of the adsorbed monolayer of omega-mercaptohexadecanoic acid. Glutaraldehyde, cyanuric chloride and phenylene diisocyanate were used to activate the amino groups of the monolayer of omega-mercaptohexadecylamine. The immobilization of albumin on the activated surface was studied by capacitive measurements. The N-hydroxysuccinimide and carbodiimide methods were identified as most suitable for protein immobilization in that they did not compromise the insulating properties of the alkylthiol layer and led to maximal increase of its dielectric thickness. These approaches were used for a layer-by-layer preparation of a capacitive immunosensor. Specifically, antibodies to human serum albumin were immobilized on the alkylthiol mono-layer. Binding of the antigen led to a decrease of the electrode capacitance. The detection limit of the immunosensor is as low as 15 nM (1 mg/l). Y1 - 1997 U6 - https://doi.org/10.1016/S0956-5663(97)00053-5 SN - 0956-5663 VL - 12 IS - 9-10 SP - 977 EP - 989 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Krause, Christian A1 - Heckmann, Klaus Dietrich T1 - Capacitive detection of adsorption/desorption on hydrophobized gold electrodes: an application for surfactants adsorption and phospholipase assay T2 - Chimica Chronica, New Series Y1 - 1996 VL - 25 SP - 179 EP - 194 ER -