TY - GEN A1 - Rehacek, Vlastimil A1 - Novotny, Ivan A1 - Tvarozek, Vladimir A1 - Riepl, Michael A1 - Hirsch, T. A1 - Mass, Markus A1 - Schweiss, R. A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. ED - Breza, Jura ED - Donoval, Daniel T1 - Capacitive detection in thin film chemical sensors and biosensors T2 - Advanced Semiconductor Devices and Microsystems N2 - Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented. Y1 - 1998 SN - 0-7803-4909-1 SP - 255 EP - 258 PB - IEEE CY - Piscataway, NJ ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Krause, Christian A1 - Novotny, Ivan A1 - Splonskowski, Markus A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Hummel, Helmut A1 - Wolfbeis, Otto S. T1 - Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry T2 - Materials Science Forum KW - Biosensor KW - Capacitance KW - Chemical Sensors KW - Electrode Y1 - 1998 U6 - https://doi.org/10.4028/www.scientific.net/MSF.287-288.423 SN - 1662-9752 VL - 287-288 SP - 423 EP - 426 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Vasjari, Majlinda A1 - Novotny, Ivan A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Wolfbeis, Otto S. T1 - Self-assembled monolayers as selective filters for chemical sensors T2 - Nanotechnology N2 - The lateral electrical conductivity of gold layers with thickness in the nanometre range was found to decrease due to adsorption of mercury, water, iodine and sulphuric compounds onto the gold surface. A self-assembled monolayer of hexadecanethiol, deposited on the gold surface, blocked the effects of water and volatile sulphuric compounds on the lateral conductivity of the gold layers, but does not block the effects of iodine and mercury. The results indicate a selective permeability of hexadecanethiol monolayers for mercury vapour, thus providing a method for development of ultrathin filters for chemical sensors. Y1 - 2002 SN - 0957-4484 VL - 13 IS - 2 SP - 175 EP - 178 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. A1 - Yastrebova, T. N. A1 - Sokolov, Valeri S. T1 - Modification of lipid bilayer properties due to sodium hypochlorite. II. Effect on model lipid systems T2 - Biological membranes Y1 - 1992 SN - 0233-4755 VL - 9 IS - 1 SP - 66 EP - 73 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Cherny, Vladimir V. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials T2 - Journal of Biochemical and Biophysical Methods N2 - To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min. Y1 - 1990 SN - 0165-022X SN - 1872-857X VL - 21 IS - 4 SP - 277 EP - 284 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Determination of the phospholipase A activity T2 - Biochemistry Y1 - 1990 VL - 55 IS - 3 SP - 445 EP - 450 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Cherny, Vladimir V. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Electrode for determination of the phospholipase activity based on the lipid membrane T2 - Soviet electrochemistry Y1 - 1989 VL - 25 SP - 1336 EP - 1340 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - BLM as an enzyme-sensitive electrode: determination of the phospholipase activity T2 - Bioelectrochemistry and Bioenergetics Y1 - 1989 SN - 0302-4598 VL - 21 IS - 3 SP - 373 EP - 378 ER - TY - GEN A1 - Shishkanova, Tatiana V. A1 - Havlik, Martin A1 - Král, Vladimír A1 - Kopecký, Dušan A1 - Matějka, Pavel A1 - Dendisová, Marcela A1 - Mirsky, Vladimir M. T1 - Amino-substituted Tröger’s base: electrochemical polymerization and characterization of the polymer film T2 - Electrochimica Acta N2 - The amino-substituted coumarin derivative of Tröger’s base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film’s conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed. KW - electrochemical polymerization KW - polymer film KW - polymeric Tröger’s base KW - nitrogen heterocycle KW - spectroscopic characterization Y1 - 2017 U6 - https://doi.org/10.1016/j.electacta.2016.12.061 SN - 0013-4686 VL - 224 SP - 439 EP - 445 ER - TY - GEN A1 - Vasjari, Majlinda A1 - Shirshov, Yuri M. A1 - Samoylov, Anton V. A1 - Mirsky, Vladimir M. T1 - SPR investigation of mercury reduction and oxidation on thin gold electrodes T2 - Journal of Electroanalytical Chemistry N2 - Mercury reduction and oxidation on ultrathin gold electrodes was investigated by simultaneous voltammetry and surface plasmon resonance (SPR). The angle dependencies of SPR signal were analyzed quantitatively. The results indicate partially reversible formation of amalgam with optical properties in between of optical properties of mercury and gold. Up to 12% content of mercury in the gold layer is suggested. KW - Surface plasmon resonance KW - Electrochemical SPR KW - Simultaneous SPR and voltammetry KW - Mercury KW - Amalgam Y1 - 2007 U6 - https://doi.org/10.1016/j.jelechem.2007.03.019 SN - 1572-6657 VL - 605 IS - 1 SP - 73 EP - 76 ER - TY - GEN A1 - Samoylov, Anton V. A1 - Mirsky, Vladimir M. A1 - Hao, Qingli A1 - Swart, Claudia A1 - Shirshov, Yuri M. A1 - Wolfbeis, Otto S. T1 - Nanometer-thick SPR sensor for gaseous HCl T2 - Sensors and Actuators B: Chemical N2 - The optical properties of electrochemically polymerized N-methylaniline are changed in the presence of gaseous hydrogen chloride. This effect was used for preparation of chemical sensors with transduction based on surface plasmon resonance. The interaction of hydrogen chloride with a two nm layer of poly-(N-methylaniline) on gold leads to the shift of the surface plasmon resonance. The analysis of the resonance spectra demonstrates that the effect is caused by an increase of the imaginary component of the refractive index; a minor contribution is also provided by film condensation leading to an increase of the real component of the refractive index and a decrease in the thickness. The effect is selective and quasi-reversible. The concentration dependence of the gas effect obeys Langmuir's adsorption isotherm with a reciprocal value of the binding constant of 850 ± 160 ppm. KW - Gas sensor KW - HCI sensors KW - Surface plasmon resonance KW - Electropolymerization KW - Poly-(N-methyl-aniline) KW - Fire detector Y1 - 2005 U6 - https://doi.org/10.1016/j.snb.2004.08.029 SN - 0925-4005 VL - 106 IS - 1 SP - 369 EP - 372 ER - TY - GEN A1 - Zynio, Stepan A. A1 - Samoylov, Anton V. A1 - Surovtseva, Elena R. A1 - Mirsky, Vladimir M. A1 - Shirshov, Yuri M. T1 - Bimetallic layers increase sensitivity of affinity sensors based on surface plasmon resonance T2 - Sensors N2 - Two metals are used in resonant layers for chemical sensors based on surface plasmon resonance (SPR) - gold and silver. Gold displays higher shift of the resonance angle to changes of ambient refraction index and is chemically stable. Silver posses narrower resonance curve thus providing a higher signal/noise ratio of SPR chemical sensors, but has a poor chemical stability. A new structure of resonant metallic film based on bimetallic silver/gold layers (gold as an outer layer) is suggested. It combines advantages of both gold and silver resonant layers. Bimetallic resonant films display so high shift of resonance angle on changes of ambient refraction index as gold films, but show narrower resonance curve, thus providing a higher signal / noise ratio. Additionally, the outer gold layer protects silver against oxidation. KW - Surface plasmon resonance KW - Biosensor KW - Sensitivity KW - Signal / noise ratio Y1 - 2002 U6 - https://doi.org/10.3390/s20200062 SN - 1424-8220 VL - 2 IS - 2 SP - 62 EP - 70 ER - TY - GEN A1 - Bajari, Tarek M. A1 - Lindstedt, Ken A. A1 - Riepl, Michael A1 - Mirsky, Vladimir M. A1 - Nimpf, Johannes A1 - Wolfbeis, Otto S. A1 - Dresel, Hans A. A1 - Bautz, Ekkehard K. F. A1 - Schneider, Wolfgang Johann T1 - A minimal binding domain of the low density lipoprotein receptor gene family T2 - Biological Chemistry KW - Ligand binding KW - Low density lipoprotein KW - Phage display KW - Receptor KW - Oocyte Y1 - 1998 U6 - https://doi.org/10.1515/bchm.1998.379.8-9.1053 SN - 1437-4315 VL - 379 IS - 8-9 SP - 1053 EP - 1062 ER - TY - GEN A1 - Scherbahn, Vitali A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - Plasmonic detection and visualization of directed adsorption of charged single nanoparticles to patterned surfaces T2 - Microchimica Acta N2 - It has recently been shown that surface plasmon microscopy (SPM) allows single nanoparticles (NPs) on sensor surfaces to be detected and analyzed. The authors have applied this technique to study the adsorption of single metallic and plastic NPs. Binding of gold NPs (40, 60 and 100 nm in size) and of 100 nm polystyrene NPs to gold surfaces modified by differently ω-functionalized alkyl thiols was studied first. Self-assembled monolayers (SAM) with varying terminal functions including amino, carboxy, oligo(ethylene glycol), methyl, or trimethylammonium groups were deposited on gold films to form surfaces possessing different charge and hydrophobicity. The affinity of NPs to these surfaces depends strongly on the type of coating. SAMs terminated with trimethylammonium groups and carboxy group display highly different affinity and therefore were preferred when creating patterned charged surfaces. Citrate-stabilized gold NPs and sulfate-terminated polystyrene NPs were used as negatively charged NPs, while branched polyethylenimine-coated silver NPs were used as positively charged NPs. It is shown that the charged patterned areas on the gold films are capable of selectively adsorbing oppositely charged NPs that can be detected and analyzed with an ~1 ng⋅mL−1 detection limit. Y1 - 2016 U6 - https://doi.org/10.1007/s00604-016-1956-7 SN - 1436-5073 VL - 183 IS - 11 SP - 2837 EP - 2845 ER - TY - GEN A1 - Pašti, Igor A. A1 - Janošević Ležaić, Aleksandra A1 - Ćirić-Marjanović, Gordana A1 - Mirsky, Vladimir M. T1 - Resistive gas sensors based on the composites of nanostructured carbonized polyaniline and Nafion T2 - Journal of Solid State Electrochemistry N2 - Due to constant necessity to have reliable and sensitive gas sensors in many contemporary technologies, there is a permanent need for development of new sensing platforms with good sensing properties. Here, we demonstrate a novel type of resistive gas sensors based on carbonized polyaniline/Nafion composites. The sensing mechanism of such sensors is based on the sorption of gases by the composites which induce Nafion swelling and decreasing of conductivity. Chemosensitive properties can be tuned by the (i) choice of carbon materials with different conductivities, (ii) Nafion content in the composite, and (iii) thickness of the composite layer. We have shown that the sensors respond to water, acetone, ethanol, and ethanol vapors. For the last two cases, we have achieved high sensitivity, fast response, wide concentration range, and good recovery. The use of simultaneous two- and four-point techniques for these sensors provides an internal control of the sensor integrity. Y1 - 2016 U6 - https://doi.org/10.1007/s10008-016-3344-y SN - 1433-0768 VL - 20 IS - 11 SP - 3061 EP - 3069 ER - TY - GEN A1 - Efremenko, Yulia A1 - Mirsky, Vladimir M. T1 - Electrically controlled variation of receptor affinity T2 - Analytical and Bioanalytical Chemistry N2 - A concept of virtual sensor array based on electrically controlled variation of affinity properties of the receptor layer is described. It was realized on the base of integrated electrochemical chemotransistor containing polyaniline as the receptor layer. Electrical control of the redox state of polyaniline was performed in five-electrode configuration containing four electrodes for conductivity measurements and one Ag/AgCl reference electrode. All the electrodes were integrated on the same glass chip. A room-temperature ionic liquid was used for the electrical connection between the reference electrode and chemosensitive material. Conductivity measurements demonstrated effective potential-controlled electrochemical conversions of the receptor material between different redox states. Binding of trimethylamine at three different potentials, corresponding to the different states of the receptor material, was studied. Concentration dependencies and binding kinetics were analyzed. The results demonstrated that the kinetic as well as the equilibrium binding properties of the receptor layer can be controlled by electrical potential, thus providing a possibility to form a virtual sensor array using only a single sensing element. Y1 - 2016 U6 - https://doi.org/10.1007/s00216-016-9751-1 SN - 1618-2650 VL - 408 IS - 26 SP - 7287 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Kasian, Olga A1 - Mirsky, Vladimir M. T1 - Individual detection and electrochemically assisted identification of adsorbed nanoparticles using surface plasmon resonance microscopy T2 - Angewandte Chemie - International Edition N2 - The increasing production and application of nanoparticles necessitates a highly sensitive analytical method for the quantification and identification of these potentially hazardous materials. We describe here an application of surface plasmon microscopy for the individual detection of each adsorbed nanoparticle and for visualization of its electrochemical conversion. Whereas the adsorption rate characterizes the number concentration of nanoparticles, the potential at which the adsorbed nanoparticles disappear during an anodic potential sweep characterizes the type of material. All the adsorbed nanoparticles are subjected to the potential sweep simultaneously; nevertheless, each of the up to a million adsorbed nanoparticles is identified individually by its electrochemical dissolution potential. The technique has been tested with silver and copper nanoparticles, but can be extended to many other electrochemically active nanomaterials. Y1 - 2016 U6 - https://doi.org/10.1002/anie.201600853 SN - 1521-3773 VL - 55 IS - 25 SP - 7247 EP - 7251 ER - TY - GEN A1 - Inel, Georgiana Anca A1 - Cioateş, Cătălina A1 - Mirsky, Vladimir M. A1 - Birzan, Liviu A1 - Ungureanu, Eleonora-Mihaela T1 - Films obtained by electrochemistry from (6-methylazulene-1-yl)selane T2 - UPB Scientific Bulletin, Series C: Electrical Engineering Y1 - 2015 SN - 1454–2331 VL - 77 IS - 2 SP - 3 EP - 18 ER - TY - GEN A1 - Janošević Ležaić, Aleksandra A1 - Bajuk-Bogdanović, Danica A1 - Radoičić, Marija A1 - Mirsky, Vladimir M. A1 - Ćirić-Marjanović, Gordana T1 - Influence of synthetic conditions on the structure and electrical properties of nanofibrous polyanilines and their nanofibrous carbonized forms T2 - Synthetic Metals N2 - Nanofibrous polyanilines (PANIs) were synthesized by several oxidative polymerization pathways that have in common the presence of excess oxidant(s) (ammonium peroxydisulfate and its mixture with hydrogen peroxide), the absence of added acid, and the absence of external template (self-assembly process). Conducting forms of the synthesized PANI nanofibers (re)doped with various acids were further used as precursors for carbonization process to obtain nanofibrous carbonaceous materials (Carb-PANIs). Morphology, molecular structure, surface properties and electrical characteristics of PANI nanofibrous precursors and their carbonized counterparts were studied by scanning electron microscopy, Raman spectroscopy, by measurements of ζ-potential and determination of isoelectric points, as well as by measurements of electrical conductivity. Y1 - 2016 U6 - https://doi.org/10.1016/j.synthmet.2016.01.015 SN - 0379-6779 VL - 214 SP - 35 EP - 44 ER - TY - GEN A1 - Sidorenko, Irina A1 - Nizamov, Shavkat A1 - Hergenröder, Roland A1 - Zybin, Alexander A1 - Kuzmichev, Alexei A1 - Kiwull, Bettina A1 - Niessner, Reinhard A1 - Mirsky, Vladimir M. T1 - Computer assisted detection and quantification of single adsorbing nanoparticles by differential surface plasmon microscopy T2 - Microchimica Acta N2 - Sensitive detection of engineered nanoparticles (NPs) in air and in liquid samples is an important task and still a major challenge in analytical chemistry. Recent work demonstrated that it can be performed using surface plasmon microscopy (SPM) where binding of single NPs to a surface leads to the formation of characteristic patterns in differential SPM images. However, these patterns have to be discriminated from a noisy background. Computer-assisted recognition of nanoparticles offers a solution but requires the development of respective tools for data analysis. Hereby a numerical method for automated detection and characterization of images of single adsorbing NPs in SPM image sequences is presented. The detection accuracy of the method was validated using computer generated images and manual counting. The method was applied for detecting and imaging of gold and silver NPs adsorbing from aqueous dispersions and for soot and NaCl NPs adsorbing from aerosols. The determined adsorption rate was in range 0.1–40 NPs per (s mm2) and linearly dependent on the concentration of nanoparticles. Depending on the type of NPs and signal to noise ratio, a probability of recognition of 90–95 % can be achieved. Y1 - 2016 U6 - https://doi.org/10.1007/s00604-015-1599-0 SN - 1436-5073 VL - 183 IS - 1 SP - 101 EP - 109 ER -