TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Ionic referencing in surface plasmon microscopy: visualization of the difference in surface properties of patterned monomolecular layers T2 - Analytical Chemistry N2 - An approach for visualization of patterned monomolecular layers in surface plasmon microscopy (SPM) is suggested. The development of hidden image in SPM is achieved by referencing of images obtained in the presence of electrolytes with a high molar refraction of either anions or cations. A formation of diffuse layer near the charged surface areas leads to the redistribution of ions. The ratio of SPM images allows one to visualize this redistribution and to distinguish surface areas with different properties. The approach is unobtrusive and robust; it can be used with most SPR imaging instruments. Y1 - 2017 U6 - https://doi.org/10.1021/acs.analchem.7b00251 SN - 0003-2700 SN - 1520-6882 VL - 89 IS - 7 SP - 3873 EP - 3878 ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Advanced wide-field surface plasmon microscopy of single adsorbing nanoparticles T2 - Optical Sensors 2017, 24–27 April 2017 Prague, Czech Republic N2 - In-situ detection and characterization of nanoparticles in biological media as well as in food or other complex samples is still a big challenge for existing analytical methods. Here we describe a label-free and cost-effective analytical method for detection of nanoparticles in the concentration range 106 -1010 NPs/ml. The proposed method is based on the surface plasmon resonance microscopy (SPRM) with a large field of view (~1.3mm2 ). It is able to detect and count adsorbing nanoparticles individually, totally up to the hundreds of thousands of NPs on the sensor surface. At constant diffusion conditions the detection rate is proportional to the number concentration of NPs, this provides an approach to determine the NPs concentration. The adsorption of nanoparticle can be manipulated by the surface functionalization, pH and electrolyte concentration of suspensions. Images of detected nanoparticles can be quantified in order to characterize them individually. The image intensity grows quasi-linearly with nanoparticle size for the given material. However, the size and material of nanoparticle cannot be resolved directly from the image. For determination of chemical composition, SPRM can be assisted by electrochemical analysis. In this case, the gold sensor surface is used both as a resonant media for plasmon microscopy and as a working electrode. Under potential sweep, the adsorbed NPs can be subjected to electrochemical dissolution, which is detected optically. The potential of this conversion characterizes the material of NPs. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267144 PB - SPIE CY - Bellingham, Washington ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Wide-field surface plasmon microscopy of nano- and microparticles: features, benchmarking, limitations, and bioanalytical applications T2 - Optical Sensors 2017, 24-27 April 2017, Prague, Czech Republic N2 - Detection of nano- and micro-particles is an important task for chemical analytics, food industry, biotechnology, environmental monitoring and many other fields of science and industry. For this purpose, a method based on the detection and analysis of minute signals in surface plasmon resonance images due to adsorption of single nanopartciles was developed. This new technology allows one a real-time detection of interaction of single nano- and micro-particles with sensor surface. Adsorption of each nanoparticle leads to characteristic diffraction image whose intensity depends on the size and chemical composition of the particle. The adsorption rate characterizes volume concentration of nano- and micro-particles. Large monitored surface area of sensor enables a high dynamic range of counting and to a correspondingly high dynamic range in concentration scale. Depending on the type of particles and experimental conditions, the detection limit for aqueous samples can be below 1000 particles per microliter. For application of method in complex media, nanoparticle images are discriminated from image perturbations due to matrix components. First, the characteristic SPRM images of nanoparticles (templates) are collected in aqueous suspensions or spiked real samples. Then, the detection of nanoparticles in complex media using template matching is performed. The detection of various NPs in consumer products like cosmetics, mineral water, juices, and wines was shown at sub-ppb level. The method can be applied for ultrasensitive detection and analysis of nano- and micro-particles of biological (bacteria, viruses, endosomes), biotechnological (liposomes, protein nanoparticles for drug delivery) or technical origin. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267158 PB - SPIE CY - Bellingham, Washington, USA ER - TY - GEN A1 - Laurinavichyute, Veronika K. A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - The Role of Anion Adsorption in the Effect of Electrode Potential on Surface Plasmon Resonance Response T2 - ChemPhysChem : a European journal of physical chemistry and chemical physics N2 - Surface plasmon resonance, being widely used in bioanalytics and biotechnology, is influenced by the electrical potential of the resonant gold layer. To evaluate the mechanism of this effect, we have studied it in solutions of various inorganic electrolytes. The magnitude of the effect decreases according to the series: KBr>KCl>KF>NaClO4. The data were treated by using different models of the interface. A quantitative description was obtained for the model, which takes into account the local dielectric function of gold being affected by the free electron charge, diffuse ionic layer near the gold/water interface, and specific adsorption of halides to the gold surface with partial charge transfer. Taking into account that most biological experiments are performed in chloride-containing solutions, detailed analysis of the model at these conditions was performed. The results indicate that the chloride adsorption is the main mechanism for the influence of potential on the surface plasmon resonance. The dependencies of surface concentration and residual charge of chloride on the applied potential were determined. Y1 - 2017 U6 - https://doi.org/10.1002/cphc.201601288 SN - 1439-7641 SN - 1439-4235 VL - 18 IS - 12 SP - 1552 EP - 1560 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Preface to the Special Issue on PCR on chip and related technologies T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1246-1 VL - 181 IS - 13/14 SP - 1609 EP - 1610 ER - TY - CHAP A1 - Scherbahn, Vitali A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. ED - Schöning, Michael J. ED - Poghossian, Arshak T1 - Toward ultrasensitive surface plasmon resonance sensors T2 - Label-free biosensing : advanced materials, devices and applications N2 - Despite the history of application of surface plasmon resonance (SPR) for chemo- and biosensing being over 30 years long, the development of this technique is still in progress. This review is focused on the technological aspects of further improvement of analytical performance of SPR transducers based on Kretschmann configuration. We describe basic measurement configurations, their improvements and optimizations, and their drawbacks and limitations. An importance of referencing in SPR sensors is highlighted. The referencing approaches are classified into the following domains: (1) macroscopic spatially separated referencing, (2) self-referencing based on micro-patterning, (3) in-place referencing, (4) spatiotemporal referencing, and (5) electrochemically assisted referencing. The underlying principles of these approaches, examples of their implementation, and resulting improvements of sensor performance are described. Finally, an analysis of SPR data and an extraction of affinity properties are discussed. KW - Self-referencing KW - Surface plasmon resonance KW - Surface plasmon resonance microscopy Y1 - 2018 SN - 978-3-319-75220-4 SN - 978-3-319-75219-8 U6 - https://doi.org/https://doi.org/10.1007/5346_2017_21 SP - 409 EP - 448 PB - Springer CY - Cham ER - TY - GEN A1 - Gutic, Sanjin J. A1 - Kozlica, Dzevad K. A1 - Bajuk-Bogdanovic, Danica A1 - Mitric, Miodrag A1 - Mirsky, Vladimir M. A1 - Mentus, Slavko V. A1 - Pasti, Igor A. T1 - Electrochemical tuning of capacitive response of graphene oxide T2 - Physical Chemistry, Chemical Physics N2 - The increasing energy demands of modern society require a deep understanding of the properties of energy storage materials, as well as the tuning of their performance. We show that the capacitance of graphene oxide (GO) can be precisely tuned using a simple electrochemical reduction route. In situ resistance measurements, in combination with cyclic voltammetry measurements and Raman spectroscopy, have shown that upon reduction GO is irreversibly deoxygenated, which is further accompanied by structural ordering and an increase in electrical conductivity. The capacitance is maximized when the concentration of oxygen functional groups is properly balanced with the conductivity. Any further reduction and deoxygenation leads to a gradual loss of capacitance. The observed trend is independent of the preparation route and the exact chemical and structural properties of GO. It is proposed that an improvement in the capacitive properties of any GO can be achieved by optimization of its reduction conditions. Y1 - 2018 U6 - https://doi.org/10.1039/C8CP03631D SN - 1463-9084 SN - 1463-9076 VL - 35 IS - 20 SP - 22698 EP - 22709 ER - TY - GEN A1 - Hammami, Asma A1 - Raouafi, Noureddine A1 - Mirsky, Vladimir M. T1 - Electrically controlled Michael addition: Addressing of covalent immobilization of biological receptors T2 - Biosensors and Bioelectronics N2 - Electrically addressed covalent immobilization of biomolecules to the defined electrodes of an electrode array is described. It is based on Michael addition of the thiol group of biomolecules to α,β-unsaturated carbonyl groups of benzoquinone. This “click” reaction was tested by immobilization of a number of thiolated compounds on the simplest array consisting of two gold electrodes coated by a self-assembled monolayer of benzoquinone-terminated hexanethiol. Electrically controlled binding of hexanethiol, ferrocenylhexanethiol, human serum albumin and thiol-terminated single-stranded DNA (ssDNA) was investigated. The binding was studied using cyclic voltammetry, X-ray photoelectron spectroscopy and surface plasmon resonance. The reaction requires the oxidized state of the benzoquinone moiety; this can be reached by applying of a moderate anodic potential to the electrode. Surface plasmon resonance measurements demonstrated that the thiol-modified ssDNA immobilized by this technique binds complementary synthetic oligonucleotides or PCR-amplified DNA fragments. The developed technology of electrical addressing of covalent immobilization can be applied for fabrication of sensor arrays. KW - Self-assembled monolayer Immobilization of biomolecules Addressable immobilization Michael addition Cyclic voltammetry Surface plasmon resonance Y1 - 2018 U6 - https://doi.org/10.1016/j.bios.2018.08.044 SN - 1873-4235 SN - 0956-5663 VL - 121 SP - 72 EP - 79 ER - TY - GEN A1 - Tonder, Karin A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Das Unsichtbare im Blick Ultrasensitive Detektion, Quantifizierung und Identifizierung industriell erzeugter und biologischer Nanopartikel T2 - Laborpraxis Y1 - 2018 UR - https://files.vogel.de/vogelonline/vogelonline/issues/lp/8263.pdf SN - 0344-1733 VL - 42 IS - 1 SP - 24 EP - 26 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - Nanodetector: Ultrasensitive plasmonic detection of single nanoparticles T2 - Compendium of Projects in the European Nanosafety Cluster Y1 - 2012 UR - https://www.nanosafetycluster.eu/home/european-nanosafety-cluster-compendium.html VL - 2012 SP - 79 EP - 83 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - Nanodetector: Ultrasensitive plasmonic detection of single nanoparticles T2 - Compendium of Projects in the European Nanosafety Cluster Y1 - 2013 UR - https://www.nanosafetycluster.eu/uploads/files/pdf/2013_NSC_Compendium.pdf VL - 2013 SP - 85 EP - 89 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - Nanodetector: Ultrasensitive plasmonic detection of single nanoparticles T2 - Compendium of Projects in the European Nanosafety Cluster Y1 - 2014 UR - https://nanoinformation.at/fileadmin_nanoinformation/_migrated/content_uploads/2014_NSC_Compendium_01.pdf VL - 2014 SP - 54 EP - 59 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - Nanodetector: Ultrasensitive plasmonic detection of single nanoparticles T2 - Compendium of Projects in the European Nanosafety Cluster Y1 - 2015 UR - https://www.nanosafetycluster.eu/uploads/files/pdf/2015_NSC_Compendium.pdf VL - 2015 SP - 83 EP - 89 ER -