TY - GEN A1 - Sokolov, Valeri S. A1 - Mirsky, Vladimir M. A1 - Toth-Boconadi, R. A1 - Markin, Vladislav S. A1 - Kesthelyi, L. T1 - Bacteriorhodopsin photocycle in decane T2 - Biological membranes Y1 - 1988 SN - 0233-4755 VL - 5 SP - 595 EP - 599 ER - TY - GEN A1 - Portnov, V. I. A1 - Mirsky, Vladimir M. A1 - Markin, Vladislav S. T1 - Current-voltage characteristic of bacteriorhodopsin T2 - Biological membranes Y1 - 1988 SN - 0233-4755 VL - 5 SP - 198 EP - 216 ER - TY - CHAP A1 - Markin, Vladislav S. A1 - Mirsky, Vladimir M. A1 - Chizmadzhev, Y. A. ED - Ovchinnikov, Yurij A. ED - Hucho, F. T1 - Photoelectrical activity of bacteriorhodopsin in planar lipid bilayer T2 - Receptors and Ion Channels, proceedings of the Symposium on Receptors and Ion Channels, Tashkent, USSR, Oct. 2 - 5, 1986 Y1 - 1987 SN - 3-11-010346-X SP - 247 EP - 254 PB - de Gruyter CY - Berlin [u.a.] ER - TY - GEN A1 - Sokolov, Valeri S. A1 - Mirsky, Vladimir M. A1 - Markin, Vladislav S. A1 - Kazarinov, V. E. T1 - Automatization of bioelectrochemical experiment T2 - Soviet electrochemistry Y1 - 1986 VL - 22 SP - 959 EP - 966 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. A1 - Chekulaeva, L. N. T1 - Bilayer lipid membrane formation from bacteriorhodopsin suspension in lipid solution T2 - Biological membranes Y1 - 1984 SN - 0233-4755 VL - 1 SP - 1143 EP - 1150 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Reconstitution of bacteriorhodopsin in bilayer lipid membrane by monolayer method T2 - Soviet electrochemistry Y1 - 1984 VL - 20 SP - 876 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Melnik, E. I. A1 - Diukova, T. V. T1 - A study of incorporation of bacteriorhodopsin into bimolecular lipid membranes T2 - Biophysics Y1 - 1984 SN - 0006-3029 VL - 29 SP - 250 EP - 253 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. T1 - Criteria of bacteriorhodopsin incorporation into bimolecular lipid membrane T2 - Biophysics Y1 - 1984 SN - 0006-3029 VL - 29 SP - 246 EP - 249 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Diukova, T. V. A1 - Melnik, E. I. T1 - A study of bacteriorhodopsin-containing proteoliposome incorporation into bimolecular lipid membranes T2 - Bioelectrochemistry and Bioenergetics N2 - The photoresponses of planar bimolecular lipid membranes (BLM) modified with liposomes containing bacteriorhodopsin (BR) were investigated in the presence of Ca 2+ cations. Short-circuit currents during switch-on and switch-off of the light were measured within a time scale exceeding the BR photocycle, and the dependence of the currents on external field and membrane conductance was studied. A comparison of the experimental data with computed values indicates that the photoresponses observed are due not only to BR contained in the proteoliposomes adhered to the BLM, as generally believed, but also to BR in BLM itself. The transfer of BR to BLM may result from the complete fusion of liposomes with the planar membrane. The existence of such a transfer is indicative of the possibility of obtaining the most convenient model system for BR studies: a planar bimolecular lipid membrane containing incorporated BR. Y1 - 1983 SN - 0302-4598 VL - 11 IS - 4-6 SP - 327 EP - 346 ER - TY - GEN A1 - Efremenko, Yulia A1 - Mirsky, Vladimir M. T1 - Virtual sensor array consisting of a single sensor element with variable affinity: an application for analysis of fish freshness T2 - Sensors and Actuators B: Chemical N2 - Recently reported concept of electrical control of sensor affinity was applied for formation of a virtual sensor array based on the single sensing element. Affinity properties of this element were modulated by electrically controlled conversion of chemosensitive material between its different redox states possessing different affinity. The sensor was realized on the basis of electrochemical chemotransistor in which a low temperature ionic liquid containing chloride was used to connect the chemosensitive material to the Ag/AgCl reference electrode. The concept of virtual array was proved by its application for monitoring of fish headspace. Using three characteristics of the sensor response measured at three different redox states of the same sensor material, we have obtained signals from a virtual sensor array consisting of nine chemosensitive elements. The sensor displays systematic changes of its nine signals during fish degradation and allows us to make quantitative analysis of its freshness defined as the after the catch. The level of detection is far below the level of organoleptic detection. KW - Chemical sensor KW - Sensor arrays KW - Affinity KW - Chemotransistor KW - Conductometric sensor KW - Conducting polymer KW - Fish freshness Y1 - 2017 U6 - https://doi.org/10.1016/j.snb.2016.10.126 SN - 0925-4005 VL - 241 SP - 652 EP - 657 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Ionic referencing in surface plasmon microscopy: visualization of the difference in surface properties of patterned monomolecular layers T2 - Analytical Chemistry N2 - An approach for visualization of patterned monomolecular layers in surface plasmon microscopy (SPM) is suggested. The development of hidden image in SPM is achieved by referencing of images obtained in the presence of electrolytes with a high molar refraction of either anions or cations. A formation of diffuse layer near the charged surface areas leads to the redistribution of ions. The ratio of SPM images allows one to visualize this redistribution and to distinguish surface areas with different properties. The approach is unobtrusive and robust; it can be used with most SPR imaging instruments. Y1 - 2017 U6 - https://doi.org/10.1021/acs.analchem.7b00251 SN - 0003-2700 SN - 1520-6882 VL - 89 IS - 7 SP - 3873 EP - 3878 ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Advanced wide-field surface plasmon microscopy of single adsorbing nanoparticles T2 - Optical Sensors 2017, 24–27 April 2017 Prague, Czech Republic N2 - In-situ detection and characterization of nanoparticles in biological media as well as in food or other complex samples is still a big challenge for existing analytical methods. Here we describe a label-free and cost-effective analytical method for detection of nanoparticles in the concentration range 106 -1010 NPs/ml. The proposed method is based on the surface plasmon resonance microscopy (SPRM) with a large field of view (~1.3mm2 ). It is able to detect and count adsorbing nanoparticles individually, totally up to the hundreds of thousands of NPs on the sensor surface. At constant diffusion conditions the detection rate is proportional to the number concentration of NPs, this provides an approach to determine the NPs concentration. The adsorption of nanoparticle can be manipulated by the surface functionalization, pH and electrolyte concentration of suspensions. Images of detected nanoparticles can be quantified in order to characterize them individually. The image intensity grows quasi-linearly with nanoparticle size for the given material. However, the size and material of nanoparticle cannot be resolved directly from the image. For determination of chemical composition, SPRM can be assisted by electrochemical analysis. In this case, the gold sensor surface is used both as a resonant media for plasmon microscopy and as a working electrode. Under potential sweep, the adsorbed NPs can be subjected to electrochemical dissolution, which is detected optically. The potential of this conversion characterizes the material of NPs. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267144 PB - SPIE CY - Bellingham, Washington ER -