TY - GEN A1 - Mirsky, Vladimir M. T1 - Fatty acids effect on the phospholipase A: electrostatical mechanism of activation T2 - Membrane and Cell Biology Y1 - 1995 SN - 1023-6597 VL - 9 SP - 175 EP - 184 ER - TY - CHAP A1 - Hayessen, R. A1 - Mirsky, Vladimir M. A1 - Heckmann, Klaus Dietrich T1 - Alkylguanidines as a base for nitrate ion sensors T2 - Sensor-95, Kongress-Band, 7. Internationale Fachmesse mit Kongreß für Sensoren, Meßaufnehmer & Systeme, 9. - 11. Mai, Nürnberg Y1 - 1995 SP - 841 EP - 844 PB - ACS CY - Wunstorf-Steinhude ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - An effect of the lipid hydrolysis products on the phospholipase A₂ action towards lipid monolayer T2 - Physics and Chemistry of Lipids N2 - The effect of lauric acid (LA) and lysolauroyllecithin (LLL) on the hydrolysis of lipid in monolayer by phospholipase A₂ from Bee venom was studied. It was found that LLL inhibits phospholipase action under both high (39 mN/m) and low (25 mN/m) surface pressure. On the other hand, LA inhibits phospholipase action under the low surface pressure (15 mN/m or 25 mN/m), but increases enzyme activity under high surface pressure (39 mN/m). This activating effect can be suppressed by high ionic strength of the aqueous subphase. It is suggested that an increase of the negative surface charge of the lipid monolayer, followed by an increase of the local concentrations of the positively charged enzyme and calcium near the monolayer is a coupling factor between fatty acid accumulation and phospholipase activation. Such an autocatalytic process can only occur when the substrate is organised into monolayer, bilayer or micelles, therefore it can be considered as a reason for the substrate activation and induction time before lipid hydrolysis. Y1 - 1994 SN - 0009-3084 VL - 70 IS - 1 SP - 75 EP - 81 ER - TY - GEN A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. T1 - Stoichiometry of the photochemical reaction leading to the damage of BLM in the presence of hematoporphyrin T2 - Biological membranes Y1 - 1993 VL - 10 IS - 1 SP - 44 EP - 49 ER - TY - GEN A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. A1 - Kayushina, R. L. A1 - Erokhin, V. V. A1 - Mironov, A. F. T1 - An interaction of hematoporphyrin dimethylether with model lipid membranes T2 - Biological membranes Y1 - 1992 VL - 9 IS - 1 SP - 74 EP - 79 ER - TY - GEN A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. A1 - Szito, T. T1 - Photosensitized by hematoporphyrin dimethylether damage of bilayer lipid membrane T2 - Biological membranes Y1 - 1991 SN - 0748-8653 VL - 8 IS - 4 SP - 412 EP - 418 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Stozhkova, I. N. A1 - Szito, T. T1 - Photosensitized damage of bilayer lipid membrane in the presence of hematoporphyrin dimethylether T2 - Journal of Photochemistry and Photobiology. B, Biology N2 - The variations in electrical conductivity and surface tension of planar bilayer lipid membranes (BLMs) sensitized by a haematoporphyrin dimethylether (HpDME) on visible light irradiation are reported. The irradiation of BLMs immediately leads to a decrease in membrane surface tension. On irradiation the conductivity of BLMs remains constant for a certain period of time (induction time), followed by an increase, terminated by membrane breakage. The induction time is not dependent on stirring of the solution, the addition of azide or ferricyanide to the solution, the addition of antioxidant to the lipid or substitution of air for argon in the cell. The induction time decreases for repeated irradiations or for any new BLM formed in the same cell immediately after the previous membrane has been broken. The conductivity shift consists of reversible and irreversible components. These results suggest that the irradiation of BLMs sensitized by HpDME leads to an accumulation of photoproducts in the membrane which induce pore formation and to a decrease in BLM stability when the concentration of the photoproducts exceeds a critical level. Y1 - 1991 SN - 1011-1344 VL - 8 IS - 3 SP - 315 EP - 324 ER - TY - GEN A1 - Portnov, V. I. A1 - Mirsky, Vladimir M. A1 - Markin, Vladislav S. T1 - Bacteriorhodopsin: current-voltage dependence T2 - Bioelectrochemistry and Bioenergetics N2 - Current-voltage dependences for different tentative bacteriorhodopsin models were studied in order to explain the linearity of this dependence observed in our previous experiments. It is proved that the best correspondence with experiment occurs when the passive proton transport path is assumed to contain many binding sites and the thickness of the widest barrier separating these sites is not more than 1 nm. One of the following two conditions is necessary: (1) electrostatic repulsion of protons in the channel is so strong that not more than one proton can be in the channel at a time; the potential energy of a proton in this channel decreases linearly towards the active site. (2) Electrostatic repulsion between protons in the channel is moderate, and the binding energy of a proton at all channel sites is equal. Under conditions of high channel occupation, single-file effects lead to non-linearity of the current-voltage characteristics if there is no electrostatic repulsion. Y1 - 1990 SN - 0302-4598 VL - 23 IS - 1 SP - 45 EP - 63 ER - TY - GEN A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. T1 - Simulation of photoradiation therapy: photosensitized effect of hematoporphyrin dimethylether on planar bilayer lipid membrane T2 - Bulletin of Experimental Biology and Medicine Y1 - 1990 SN - 0007-4888 SN - 1573-8221 VL - 110 IS - 1 SP - 898 EP - 900 ER - TY - GEN A1 - Sokolov, Valeri S. A1 - Mirsky, Vladimir M. A1 - Toth-Boconadi, R. A1 - Markin, Vladislav S. A1 - Kesthelyi, L. T1 - Bacteriorhodopsin photocycle in decane T2 - Biological membranes Y1 - 1988 SN - 0233-4755 VL - 5 SP - 595 EP - 599 ER - TY - GEN A1 - Portnov, V. I. A1 - Mirsky, Vladimir M. A1 - Markin, Vladislav S. T1 - Current-voltage characteristic of bacteriorhodopsin T2 - Biological membranes Y1 - 1988 SN - 0233-4755 VL - 5 SP - 198 EP - 216 ER - TY - CHAP A1 - Markin, Vladislav S. A1 - Mirsky, Vladimir M. A1 - Chizmadzhev, Y. A. ED - Ovchinnikov, Yurij A. ED - Hucho, F. T1 - Photoelectrical activity of bacteriorhodopsin in planar lipid bilayer T2 - Receptors and Ion Channels, proceedings of the Symposium on Receptors and Ion Channels, Tashkent, USSR, Oct. 2 - 5, 1986 Y1 - 1987 SN - 3-11-010346-X SP - 247 EP - 254 PB - de Gruyter CY - Berlin [u.a.] ER - TY - GEN A1 - Sokolov, Valeri S. A1 - Mirsky, Vladimir M. A1 - Markin, Vladislav S. A1 - Kazarinov, V. E. T1 - Automatization of bioelectrochemical experiment T2 - Soviet electrochemistry Y1 - 1986 VL - 22 SP - 959 EP - 966 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. A1 - Chekulaeva, L. N. T1 - Bilayer lipid membrane formation from bacteriorhodopsin suspension in lipid solution T2 - Biological membranes Y1 - 1984 SN - 0233-4755 VL - 1 SP - 1143 EP - 1150 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Reconstitution of bacteriorhodopsin in bilayer lipid membrane by monolayer method T2 - Soviet electrochemistry Y1 - 1984 VL - 20 SP - 876 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Melnik, E. I. A1 - Diukova, T. V. T1 - A study of incorporation of bacteriorhodopsin into bimolecular lipid membranes T2 - Biophysics Y1 - 1984 SN - 0006-3029 VL - 29 SP - 250 EP - 253 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. T1 - Criteria of bacteriorhodopsin incorporation into bimolecular lipid membrane T2 - Biophysics Y1 - 1984 SN - 0006-3029 VL - 29 SP - 246 EP - 249 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Diukova, T. V. A1 - Melnik, E. I. T1 - A study of bacteriorhodopsin-containing proteoliposome incorporation into bimolecular lipid membranes T2 - Bioelectrochemistry and Bioenergetics N2 - The photoresponses of planar bimolecular lipid membranes (BLM) modified with liposomes containing bacteriorhodopsin (BR) were investigated in the presence of Ca 2+ cations. Short-circuit currents during switch-on and switch-off of the light were measured within a time scale exceeding the BR photocycle, and the dependence of the currents on external field and membrane conductance was studied. A comparison of the experimental data with computed values indicates that the photoresponses observed are due not only to BR contained in the proteoliposomes adhered to the BLM, as generally believed, but also to BR in BLM itself. The transfer of BR to BLM may result from the complete fusion of liposomes with the planar membrane. The existence of such a transfer is indicative of the possibility of obtaining the most convenient model system for BR studies: a planar bimolecular lipid membrane containing incorporated BR. Y1 - 1983 SN - 0302-4598 VL - 11 IS - 4-6 SP - 327 EP - 346 ER - TY - GEN A1 - Efremenko, Yulia A1 - Mirsky, Vladimir M. T1 - Virtual sensor array consisting of a single sensor element with variable affinity: an application for analysis of fish freshness T2 - Sensors and Actuators B: Chemical N2 - Recently reported concept of electrical control of sensor affinity was applied for formation of a virtual sensor array based on the single sensing element. Affinity properties of this element were modulated by electrically controlled conversion of chemosensitive material between its different redox states possessing different affinity. The sensor was realized on the basis of electrochemical chemotransistor in which a low temperature ionic liquid containing chloride was used to connect the chemosensitive material to the Ag/AgCl reference electrode. The concept of virtual array was proved by its application for monitoring of fish headspace. Using three characteristics of the sensor response measured at three different redox states of the same sensor material, we have obtained signals from a virtual sensor array consisting of nine chemosensitive elements. The sensor displays systematic changes of its nine signals during fish degradation and allows us to make quantitative analysis of its freshness defined as the after the catch. The level of detection is far below the level of organoleptic detection. KW - Chemical sensor KW - Sensor arrays KW - Affinity KW - Chemotransistor KW - Conductometric sensor KW - Conducting polymer KW - Fish freshness Y1 - 2017 U6 - https://doi.org/10.1016/j.snb.2016.10.126 SN - 0925-4005 VL - 241 SP - 652 EP - 657 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Ionic referencing in surface plasmon microscopy: visualization of the difference in surface properties of patterned monomolecular layers T2 - Analytical Chemistry N2 - An approach for visualization of patterned monomolecular layers in surface plasmon microscopy (SPM) is suggested. The development of hidden image in SPM is achieved by referencing of images obtained in the presence of electrolytes with a high molar refraction of either anions or cations. A formation of diffuse layer near the charged surface areas leads to the redistribution of ions. The ratio of SPM images allows one to visualize this redistribution and to distinguish surface areas with different properties. The approach is unobtrusive and robust; it can be used with most SPR imaging instruments. Y1 - 2017 U6 - https://doi.org/10.1021/acs.analchem.7b00251 SN - 0003-2700 SN - 1520-6882 VL - 89 IS - 7 SP - 3873 EP - 3878 ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Advanced wide-field surface plasmon microscopy of single adsorbing nanoparticles T2 - Optical Sensors 2017, 24–27 April 2017 Prague, Czech Republic N2 - In-situ detection and characterization of nanoparticles in biological media as well as in food or other complex samples is still a big challenge for existing analytical methods. Here we describe a label-free and cost-effective analytical method for detection of nanoparticles in the concentration range 106 -1010 NPs/ml. The proposed method is based on the surface plasmon resonance microscopy (SPRM) with a large field of view (~1.3mm2 ). It is able to detect and count adsorbing nanoparticles individually, totally up to the hundreds of thousands of NPs on the sensor surface. At constant diffusion conditions the detection rate is proportional to the number concentration of NPs, this provides an approach to determine the NPs concentration. The adsorption of nanoparticle can be manipulated by the surface functionalization, pH and electrolyte concentration of suspensions. Images of detected nanoparticles can be quantified in order to characterize them individually. The image intensity grows quasi-linearly with nanoparticle size for the given material. However, the size and material of nanoparticle cannot be resolved directly from the image. For determination of chemical composition, SPRM can be assisted by electrochemical analysis. In this case, the gold sensor surface is used both as a resonant media for plasmon microscopy and as a working electrode. Under potential sweep, the adsorbed NPs can be subjected to electrochemical dissolution, which is detected optically. The potential of this conversion characterizes the material of NPs. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267144 PB - SPIE CY - Bellingham, Washington ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Wide-field surface plasmon microscopy of nano- and microparticles: features, benchmarking, limitations, and bioanalytical applications T2 - Optical Sensors 2017, 24-27 April 2017, Prague, Czech Republic N2 - Detection of nano- and micro-particles is an important task for chemical analytics, food industry, biotechnology, environmental monitoring and many other fields of science and industry. For this purpose, a method based on the detection and analysis of minute signals in surface plasmon resonance images due to adsorption of single nanopartciles was developed. This new technology allows one a real-time detection of interaction of single nano- and micro-particles with sensor surface. Adsorption of each nanoparticle leads to characteristic diffraction image whose intensity depends on the size and chemical composition of the particle. The adsorption rate characterizes volume concentration of nano- and micro-particles. Large monitored surface area of sensor enables a high dynamic range of counting and to a correspondingly high dynamic range in concentration scale. Depending on the type of particles and experimental conditions, the detection limit for aqueous samples can be below 1000 particles per microliter. For application of method in complex media, nanoparticle images are discriminated from image perturbations due to matrix components. First, the characteristic SPRM images of nanoparticles (templates) are collected in aqueous suspensions or spiked real samples. Then, the detection of nanoparticles in complex media using template matching is performed. The detection of various NPs in consumer products like cosmetics, mineral water, juices, and wines was shown at sub-ppb level. The method can be applied for ultrasensitive detection and analysis of nano- and micro-particles of biological (bacteria, viruses, endosomes), biotechnological (liposomes, protein nanoparticles for drug delivery) or technical origin. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267158 PB - SPIE CY - Bellingham, Washington, USA ER -