TY - GEN A1 - Cherny, Vladimir V. A1 - Sihuralidze, M. G. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. T1 - An effect of the phospholipase A2 on the electrical potential distribution through lipid bilayer T2 - Biological membranes Y1 - 1992 VL - 7 SP - 733 EP - 740 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Donath, E. A1 - Mirsky, Vladimir M. A1 - Paulitchke, M. A1 - Sokolov, Valeri S. T1 - Modification of lipid bilayer properties due to sodium hypochlorite. I. Effect on red blood cell membranes T2 - Biological membranes Y1 - 1992 VL - 9 IS - 1 SP - 60 EP - 65 ER - TY - PAT A1 - Mirsky, Vladimir M. A1 - Mirsky, Alex T1 - Distributed reference spots in chemical sensing Y1 - 2006 ER - TY - GEN A1 - Lyutov, Vladimir V. A1 - Ivanov, Svetlozar D. A1 - Mirsky, Vladimir M. A1 - Tsakova, Vessela T. T1 - Polyaniline doped with poly(acrylamidomethylpropanesulphonic acid): electrochemical behaviour and conductive properties in neutral solutions T2 - Chemical Papers N2 - Poly(2-acrylamido-2-methyl-1-propanesulphonic acid) (PAMPSA)-doped polyaniline (PANI) layers are synthesised in the presence of sulphuric and perchloric acids. The effects of the inorganic acid as well as of the electrochemical synthetic procedure (potentiostatic and potentiodynamic deposition) and thickness of the polymer layers are studied. The focus is directed towards the pH dependence of the electrochemical redox activity and conductivity of the PAMPSA-doped PANI layers obtained under different conditions. Ascorbic acid oxidation is used as a test reaction to study the electrocatalytic behaviour of various PAMPSA-doped PANI layers in neutral solution. It is found that the type of inorganic component present in the polymerisation solution has a marked effect on the extent of doping in acidic solutions as well as on the redox electroactivity in neutral solutions. A comparison between potentiostatically and potentiodynamically synthesised layers at pH 7 shows a markedly lower conductance and lower extent of redox charge preservation in the case of potentiodynamic synthesis. The PANI electrocatalytic activity for ascorbic acid oxidation is also dependent on the polymer electrodeposition procedure, with potentiostatically synthesised layers exhibiting better electrocatalytic performance. KW - polyaniline KW - polyacids KW - PAMPSA KW - conductance KW - electrochemical Y1 - 2013 U6 - https://doi.org/10.2478/s11696-013-0341-9 SN - 1336-9075 VL - 67 IS - 8 SP - 1002 EP - 1011 ER - TY - GEN A1 - Tsakova, Vessela T. A1 - Ivanov, Svetlozar D. A1 - Lange, Ulrich A1 - Stoyanova, Aneliya A1 - Lyutov, Vladimir V. A1 - Mirsky, Vladimir M. T1 - Electroanalytical applications of nanocomposites from conducting polymers and metallic nanoparticles prepared by layer-by-layer deposition T2 - Pure and Applied Chemistry N2 - Layer-by-layer (LbL) deposition is a convenient technique for the formation of ultra-thin nanocomposite layers containing metallic nanoparticles (NPs) and conducting polymers (CPs). The advantages of this approach for producing composite layers suitable for electroanalytical applications are discussed. Examples of electroanalytical applications of LbL-deposited composites are presented. Composite layers consisting of polyaniline (PANI) and Pd NPs are used for hydrazine oxidation. The PANI–Au NPs system is applied for dopamine (DA) and uric acid (UA) oxidation. KW - conducting polymers KW - dopamine KW - electrochemistry KW - hydrazine KW - layer-by-layer deposition KW - nanoparticles KW - polyaniline (PANI) KW - uric acid Y1 - 2010 U6 - https://doi.org/10.1351/PAC-CON-10-08-01 SN - 1365-3075 VL - 83 IS - 2 SP - 345 EP - 358 ER - TY - GEN A1 - Broncová, Gabriela A1 - Anikin, Sergey A1 - Lange, Ulrich A1 - Matějka, Pavel A1 - Krondak, Martin A1 - Král, Vladimír A1 - Mirsky, Vladimir M. T1 - Electrochemical and spectroscopic properties of poly-4,4′-dialkoxy-2,2′-bipyrroles T2 - Journal of Solid State Electrochemistry N2 - The structure and the electrochemical and spectral properties of two conductive electrochemically polymerized substituted bipyrroles 4,4′-methoxy-2,2′-bipyrrole and 4,4′-buthoxy-2,2′-bipyrrole were studied and compared. The polymers were characterized by cyclic voltammetry, FT-Raman spectroscopy, scanning electron microscopy, and in situ conductivity measurements at different pH and redox state. KW - Conducting polymer KW - Polydialkoxybipyrroles KW - Raman spectroscopy KW - Cyclic voltammetry KW - Conductivity Y1 - 2010 U6 - https://doi.org/10.1007/s10008-009-0911-5 SN - 1432-8488 SN - 1433-0768 VL - 14 IS - 6 SP - 1035 EP - 1044 ER - TY - GEN A1 - Lange, Ulrich A1 - Ivanov, Svetlozar D. A1 - Lyutov, Vladimir V. A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Voltammetric and conductometric behaviour of self assembled multilayer nanocomposites of polyaniline and gold nanoparticles T2 - Journal of Solid State Electrochemistry N2 - Multilayer nanocomposites from polyaniline (PANI) and gold nanoparticles (AuNPs) were formed by layer-by-layer deposition. The formation of PANI–AuNPs multilayer structures was monitored by UV-vis absorption spectroscopy and cyclic voltammetry. Each deposited bilayer of PANI–AuNPs led to a monotonous and almost linear increase in both optical absorbance and the first current peak of PANI oxidation. The prepared multilayer nanocomposites were characterized by in situ conductivity measurements at different pH and potential and by transmission electron microscopy. Finally, chemosensitive properties of the new material based on the intrinsic affinity of gold nanoparticles were studied. Changes in the film resistance on exposure to vapors of mercury and sulfur-containing compounds were observed. KW - Polyaniline KW - Gold nanoparticles KW - Layer-by-layer deposition KW - Chemoresistor KW - In situ conductivity measurements KW - Nanocomposite Y1 - 2010 U6 - https://doi.org/10.1007/s10008-009-0922-2 SN - 1432-8488 SN - 1433-0768 VL - 14 IS - 7 SP - 1261 EP - 1268 ER - TY - GEN A1 - Cígler, Petr A1 - Král, Vladimír A1 - Kožíšek, Milan A1 - Konvalinka, Jan A1 - Mirsky, Vladimir M. T1 - Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization? T2 - Colloids and Surfaces B: Biointerfaces N2 - Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices. KW - HIV protease KW - Protein adsorption KW - Protein-resistant surfaces KW - Self-assembled monolayer KW - Surface plasmon resonance (SPR) Y1 - 2008 U6 - https://doi.org/10.1016/j.colsurfb.2008.01.011 SN - 0927-7765 VL - 64 IS - 1 SP - 145 EP - 149 ER - TY - GEN A1 - Riepl, Michael A1 - Mirsky, Vladimir M. A1 - Novotny, Ivan A1 - Tvarozek, Vladimir A1 - Rehacek, Vlastimil A1 - Wolfbeis, Otto S. T1 - Optimization of capacitive affinity sensors: drift suppression and signal amplification T2 - Analytica Chimica Acta N2 - The detection limit of capacitive affinity sensors based on the gold–alkanethiol system can be improved by optimization of sensor preparation and by signal amplification. The dissociation of the gold–sulfur binding is often a critical point leading to operative errors of such sensors. The stability of self-assembled monolayers prepared with different thiols on gold electrodes in aqueous and organic solvents was studied by the capacitive technique. The results show that monolayers made of 16-mercaptohexadecanoic acid are stable in aqueous solution and can be hardly extracted from a gold surface by ethanol, methanol, or dioxane, while a considerable damage of self-assembled monolayers was observed due to incubation in chloroform or dimethylformamide. In contrast, self-assembled monolayers made from short-chain disulfides or thiols (such as 3,3′-dithio-bis(propionic acid N-hydroxysuccinimide ester) or 11-mercaptoundecanoic acid) displayed a poor stability in aqueous phase. Capacitive affinity sensors based on these short-chain thiols showed a considerable drift of the signal. The use of long-chain thiols resulted in a stable signal; it was applied to compare capacitive effects due to immobilization of different biological molecules and for preparation of different biosensors. The response of capacitive biosensors can be amplified by formation of a sandwich structure. This principle was illustrated by subsequent adsorption of polyclonal anti-HSA after binding of HSA with a sensor for HSA based on monoclonal antibodies. KW - Affinity sensor KW - Self-assembly KW - Capacitive sensor KW - Signal amplification KW - Immunosensor KW - Biosensor Y1 - 1999 U6 - https://doi.org/10.1016/S0003-2670(99)00195-6 SN - 0003-2670 VL - 392 IS - 1 SP - 77 EP - 84 ER - TY - GEN A1 - Rehacek, Vlastimil A1 - Novotny, Ivan A1 - Tvarozek, Vladimir A1 - Riepl, Michael A1 - Hirsch, T. A1 - Mass, Markus A1 - Schweiss, R. A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. ED - Breza, Jura ED - Donoval, Daniel T1 - Capacitive detection in thin film chemical sensors and biosensors T2 - Advanced Semiconductor Devices and Microsystems N2 - Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented. Y1 - 1998 SN - 0-7803-4909-1 SP - 255 EP - 258 PB - IEEE CY - Piscataway, NJ ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Krause, Christian A1 - Novotny, Ivan A1 - Splonskowski, Markus A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Hummel, Helmut A1 - Wolfbeis, Otto S. T1 - Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry T2 - Materials Science Forum KW - Biosensor KW - Capacitance KW - Chemical Sensors KW - Electrode Y1 - 1998 U6 - https://doi.org/10.4028/www.scientific.net/MSF.287-288.423 SN - 1662-9752 VL - 287-288 SP - 423 EP - 426 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Vasjari, Majlinda A1 - Novotny, Ivan A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Wolfbeis, Otto S. T1 - Self-assembled monolayers as selective filters for chemical sensors T2 - Nanotechnology N2 - The lateral electrical conductivity of gold layers with thickness in the nanometre range was found to decrease due to adsorption of mercury, water, iodine and sulphuric compounds onto the gold surface. A self-assembled monolayer of hexadecanethiol, deposited on the gold surface, blocked the effects of water and volatile sulphuric compounds on the lateral conductivity of the gold layers, but does not block the effects of iodine and mercury. The results indicate a selective permeability of hexadecanethiol monolayers for mercury vapour, thus providing a method for development of ultrathin filters for chemical sensors. Y1 - 2002 SN - 0957-4484 VL - 13 IS - 2 SP - 175 EP - 178 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. A1 - Yastrebova, T. N. A1 - Sokolov, Valeri S. T1 - Modification of lipid bilayer properties due to sodium hypochlorite. II. Effect on model lipid systems T2 - Biological membranes Y1 - 1992 SN - 0233-4755 VL - 9 IS - 1 SP - 66 EP - 73 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Cherny, Vladimir V. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials T2 - Journal of Biochemical and Biophysical Methods N2 - To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min. Y1 - 1990 SN - 0165-022X SN - 1872-857X VL - 21 IS - 4 SP - 277 EP - 284 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Determination of the phospholipase A activity T2 - Biochemistry Y1 - 1990 VL - 55 IS - 3 SP - 445 EP - 450 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Cherny, Vladimir V. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Electrode for determination of the phospholipase activity based on the lipid membrane T2 - Soviet electrochemistry Y1 - 1989 VL - 25 SP - 1336 EP - 1340 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - BLM as an enzyme-sensitive electrode: determination of the phospholipase activity T2 - Bioelectrochemistry and Bioenergetics Y1 - 1989 SN - 0302-4598 VL - 21 IS - 3 SP - 373 EP - 378 ER - TY - GEN A1 - Shishkanova, Tatiana V. A1 - Havlik, Martin A1 - Král, Vladimír A1 - Kopecký, Dušan A1 - Matějka, Pavel A1 - Dendisová, Marcela A1 - Mirsky, Vladimir M. T1 - Amino-substituted Tröger’s base: electrochemical polymerization and characterization of the polymer film T2 - Electrochimica Acta N2 - The amino-substituted coumarin derivative of Tröger’s base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film’s conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed. KW - electrochemical polymerization KW - polymer film KW - polymeric Tröger’s base KW - nitrogen heterocycle KW - spectroscopic characterization Y1 - 2017 U6 - https://doi.org/10.1016/j.electacta.2016.12.061 SN - 0013-4686 VL - 224 SP - 439 EP - 445 ER - TY - GEN A1 - Vasjari, Majlinda A1 - Shirshov, Yuri M. A1 - Samoylov, Anton V. A1 - Mirsky, Vladimir M. T1 - SPR investigation of mercury reduction and oxidation on thin gold electrodes T2 - Journal of Electroanalytical Chemistry N2 - Mercury reduction and oxidation on ultrathin gold electrodes was investigated by simultaneous voltammetry and surface plasmon resonance (SPR). The angle dependencies of SPR signal were analyzed quantitatively. The results indicate partially reversible formation of amalgam with optical properties in between of optical properties of mercury and gold. Up to 12% content of mercury in the gold layer is suggested. KW - Surface plasmon resonance KW - Electrochemical SPR KW - Simultaneous SPR and voltammetry KW - Mercury KW - Amalgam Y1 - 2007 U6 - https://doi.org/10.1016/j.jelechem.2007.03.019 SN - 1572-6657 VL - 605 IS - 1 SP - 73 EP - 76 ER - TY - GEN A1 - Samoylov, Anton V. A1 - Mirsky, Vladimir M. A1 - Hao, Qingli A1 - Swart, Claudia A1 - Shirshov, Yuri M. A1 - Wolfbeis, Otto S. T1 - Nanometer-thick SPR sensor for gaseous HCl T2 - Sensors and Actuators B: Chemical N2 - The optical properties of electrochemically polymerized N-methylaniline are changed in the presence of gaseous hydrogen chloride. This effect was used for preparation of chemical sensors with transduction based on surface plasmon resonance. The interaction of hydrogen chloride with a two nm layer of poly-(N-methylaniline) on gold leads to the shift of the surface plasmon resonance. The analysis of the resonance spectra demonstrates that the effect is caused by an increase of the imaginary component of the refractive index; a minor contribution is also provided by film condensation leading to an increase of the real component of the refractive index and a decrease in the thickness. The effect is selective and quasi-reversible. The concentration dependence of the gas effect obeys Langmuir's adsorption isotherm with a reciprocal value of the binding constant of 850 ± 160 ppm. KW - Gas sensor KW - HCI sensors KW - Surface plasmon resonance KW - Electropolymerization KW - Poly-(N-methyl-aniline) KW - Fire detector Y1 - 2005 U6 - https://doi.org/10.1016/j.snb.2004.08.029 SN - 0925-4005 VL - 106 IS - 1 SP - 369 EP - 372 ER - TY - GEN A1 - Zynio, Stepan A. A1 - Samoylov, Anton V. A1 - Surovtseva, Elena R. A1 - Mirsky, Vladimir M. A1 - Shirshov, Yuri M. T1 - Bimetallic layers increase sensitivity of affinity sensors based on surface plasmon resonance T2 - Sensors N2 - Two metals are used in resonant layers for chemical sensors based on surface plasmon resonance (SPR) - gold and silver. Gold displays higher shift of the resonance angle to changes of ambient refraction index and is chemically stable. Silver posses narrower resonance curve thus providing a higher signal/noise ratio of SPR chemical sensors, but has a poor chemical stability. A new structure of resonant metallic film based on bimetallic silver/gold layers (gold as an outer layer) is suggested. It combines advantages of both gold and silver resonant layers. Bimetallic resonant films display so high shift of resonance angle on changes of ambient refraction index as gold films, but show narrower resonance curve, thus providing a higher signal / noise ratio. Additionally, the outer gold layer protects silver against oxidation. KW - Surface plasmon resonance KW - Biosensor KW - Sensitivity KW - Signal / noise ratio Y1 - 2002 U6 - https://doi.org/10.3390/s20200062 SN - 1424-8220 VL - 2 IS - 2 SP - 62 EP - 70 ER - TY - GEN A1 - Bajari, Tarek M. A1 - Lindstedt, Ken A. A1 - Riepl, Michael A1 - Mirsky, Vladimir M. A1 - Nimpf, Johannes A1 - Wolfbeis, Otto S. A1 - Dresel, Hans A. A1 - Bautz, Ekkehard K. F. A1 - Schneider, Wolfgang Johann T1 - A minimal binding domain of the low density lipoprotein receptor gene family T2 - Biological Chemistry KW - Ligand binding KW - Low density lipoprotein KW - Phage display KW - Receptor KW - Oocyte Y1 - 1998 U6 - https://doi.org/10.1515/bchm.1998.379.8-9.1053 SN - 1437-4315 VL - 379 IS - 8-9 SP - 1053 EP - 1062 ER - TY - GEN A1 - Scherbahn, Vitali A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - Plasmonic detection and visualization of directed adsorption of charged single nanoparticles to patterned surfaces T2 - Microchimica Acta N2 - It has recently been shown that surface plasmon microscopy (SPM) allows single nanoparticles (NPs) on sensor surfaces to be detected and analyzed. The authors have applied this technique to study the adsorption of single metallic and plastic NPs. Binding of gold NPs (40, 60 and 100 nm in size) and of 100 nm polystyrene NPs to gold surfaces modified by differently ω-functionalized alkyl thiols was studied first. Self-assembled monolayers (SAM) with varying terminal functions including amino, carboxy, oligo(ethylene glycol), methyl, or trimethylammonium groups were deposited on gold films to form surfaces possessing different charge and hydrophobicity. The affinity of NPs to these surfaces depends strongly on the type of coating. SAMs terminated with trimethylammonium groups and carboxy group display highly different affinity and therefore were preferred when creating patterned charged surfaces. Citrate-stabilized gold NPs and sulfate-terminated polystyrene NPs were used as negatively charged NPs, while branched polyethylenimine-coated silver NPs were used as positively charged NPs. It is shown that the charged patterned areas on the gold films are capable of selectively adsorbing oppositely charged NPs that can be detected and analyzed with an ~1 ng⋅mL−1 detection limit. Y1 - 2016 U6 - https://doi.org/10.1007/s00604-016-1956-7 SN - 1436-5073 VL - 183 IS - 11 SP - 2837 EP - 2845 ER - TY - GEN A1 - Pašti, Igor A. A1 - Janošević Ležaić, Aleksandra A1 - Ćirić-Marjanović, Gordana A1 - Mirsky, Vladimir M. T1 - Resistive gas sensors based on the composites of nanostructured carbonized polyaniline and Nafion T2 - Journal of Solid State Electrochemistry N2 - Due to constant necessity to have reliable and sensitive gas sensors in many contemporary technologies, there is a permanent need for development of new sensing platforms with good sensing properties. Here, we demonstrate a novel type of resistive gas sensors based on carbonized polyaniline/Nafion composites. The sensing mechanism of such sensors is based on the sorption of gases by the composites which induce Nafion swelling and decreasing of conductivity. Chemosensitive properties can be tuned by the (i) choice of carbon materials with different conductivities, (ii) Nafion content in the composite, and (iii) thickness of the composite layer. We have shown that the sensors respond to water, acetone, ethanol, and ethanol vapors. For the last two cases, we have achieved high sensitivity, fast response, wide concentration range, and good recovery. The use of simultaneous two- and four-point techniques for these sensors provides an internal control of the sensor integrity. Y1 - 2016 U6 - https://doi.org/10.1007/s10008-016-3344-y SN - 1433-0768 VL - 20 IS - 11 SP - 3061 EP - 3069 ER - TY - GEN A1 - Efremenko, Yulia A1 - Mirsky, Vladimir M. T1 - Electrically controlled variation of receptor affinity T2 - Analytical and Bioanalytical Chemistry N2 - A concept of virtual sensor array based on electrically controlled variation of affinity properties of the receptor layer is described. It was realized on the base of integrated electrochemical chemotransistor containing polyaniline as the receptor layer. Electrical control of the redox state of polyaniline was performed in five-electrode configuration containing four electrodes for conductivity measurements and one Ag/AgCl reference electrode. All the electrodes were integrated on the same glass chip. A room-temperature ionic liquid was used for the electrical connection between the reference electrode and chemosensitive material. Conductivity measurements demonstrated effective potential-controlled electrochemical conversions of the receptor material between different redox states. Binding of trimethylamine at three different potentials, corresponding to the different states of the receptor material, was studied. Concentration dependencies and binding kinetics were analyzed. The results demonstrated that the kinetic as well as the equilibrium binding properties of the receptor layer can be controlled by electrical potential, thus providing a possibility to form a virtual sensor array using only a single sensing element. Y1 - 2016 U6 - https://doi.org/10.1007/s00216-016-9751-1 SN - 1618-2650 VL - 408 IS - 26 SP - 7287 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Kasian, Olga A1 - Mirsky, Vladimir M. T1 - Individual detection and electrochemically assisted identification of adsorbed nanoparticles using surface plasmon resonance microscopy T2 - Angewandte Chemie - International Edition N2 - The increasing production and application of nanoparticles necessitates a highly sensitive analytical method for the quantification and identification of these potentially hazardous materials. We describe here an application of surface plasmon microscopy for the individual detection of each adsorbed nanoparticle and for visualization of its electrochemical conversion. Whereas the adsorption rate characterizes the number concentration of nanoparticles, the potential at which the adsorbed nanoparticles disappear during an anodic potential sweep characterizes the type of material. All the adsorbed nanoparticles are subjected to the potential sweep simultaneously; nevertheless, each of the up to a million adsorbed nanoparticles is identified individually by its electrochemical dissolution potential. The technique has been tested with silver and copper nanoparticles, but can be extended to many other electrochemically active nanomaterials. Y1 - 2016 U6 - https://doi.org/10.1002/anie.201600853 SN - 1521-3773 VL - 55 IS - 25 SP - 7247 EP - 7251 ER - TY - GEN A1 - Inel, Georgiana Anca A1 - Cioateş, Cătălina A1 - Mirsky, Vladimir M. A1 - Birzan, Liviu A1 - Ungureanu, Eleonora-Mihaela T1 - Films obtained by electrochemistry from (6-methylazulene-1-yl)selane T2 - UPB Scientific Bulletin, Series C: Electrical Engineering Y1 - 2015 SN - 1454–2331 VL - 77 IS - 2 SP - 3 EP - 18 ER - TY - GEN A1 - Janošević Ležaić, Aleksandra A1 - Bajuk-Bogdanović, Danica A1 - Radoičić, Marija A1 - Mirsky, Vladimir M. A1 - Ćirić-Marjanović, Gordana T1 - Influence of synthetic conditions on the structure and electrical properties of nanofibrous polyanilines and their nanofibrous carbonized forms T2 - Synthetic Metals N2 - Nanofibrous polyanilines (PANIs) were synthesized by several oxidative polymerization pathways that have in common the presence of excess oxidant(s) (ammonium peroxydisulfate and its mixture with hydrogen peroxide), the absence of added acid, and the absence of external template (self-assembly process). Conducting forms of the synthesized PANI nanofibers (re)doped with various acids were further used as precursors for carbonization process to obtain nanofibrous carbonaceous materials (Carb-PANIs). Morphology, molecular structure, surface properties and electrical characteristics of PANI nanofibrous precursors and their carbonized counterparts were studied by scanning electron microscopy, Raman spectroscopy, by measurements of ζ-potential and determination of isoelectric points, as well as by measurements of electrical conductivity. Y1 - 2016 U6 - https://doi.org/10.1016/j.synthmet.2016.01.015 SN - 0379-6779 VL - 214 SP - 35 EP - 44 ER - TY - GEN A1 - Sidorenko, Irina A1 - Nizamov, Shavkat A1 - Hergenröder, Roland A1 - Zybin, Alexander A1 - Kuzmichev, Alexei A1 - Kiwull, Bettina A1 - Niessner, Reinhard A1 - Mirsky, Vladimir M. T1 - Computer assisted detection and quantification of single adsorbing nanoparticles by differential surface plasmon microscopy T2 - Microchimica Acta N2 - Sensitive detection of engineered nanoparticles (NPs) in air and in liquid samples is an important task and still a major challenge in analytical chemistry. Recent work demonstrated that it can be performed using surface plasmon microscopy (SPM) where binding of single NPs to a surface leads to the formation of characteristic patterns in differential SPM images. However, these patterns have to be discriminated from a noisy background. Computer-assisted recognition of nanoparticles offers a solution but requires the development of respective tools for data analysis. Hereby a numerical method for automated detection and characterization of images of single adsorbing NPs in SPM image sequences is presented. The detection accuracy of the method was validated using computer generated images and manual counting. The method was applied for detecting and imaging of gold and silver NPs adsorbing from aqueous dispersions and for soot and NaCl NPs adsorbing from aerosols. The determined adsorption rate was in range 0.1–40 NPs per (s mm2) and linearly dependent on the concentration of nanoparticles. Depending on the type of NPs and signal to noise ratio, a probability of recognition of 90–95 % can be achieved. Y1 - 2016 U6 - https://doi.org/10.1007/s00604-015-1599-0 SN - 1436-5073 VL - 183 IS - 1 SP - 101 EP - 109 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Self-referencing SPR-sensor based on integral measurements of light intensity reflected by arbitrarily distributed sensing and referencing spots T2 - Sensors and Actuators B: Chemical N2 - A new approach for self-referencing in SPR biosensors is reported. The method is technologically simple and applicable for a wide range of existing SPR instrumentation with Kretschmann configuration. It is based on the micropatterning of the sensor area with sensing and referencing areas whose shape and distribution can be chosen arbitrarily and their characteristic sizes are larger than the plasmons propagation length. Provided that roughly a half of the area is used as the sensing area while its optical thickness is different from that of the referencing area, an integral measurement of the intensity of the reflected light over such a patterned surface near the summary resonance conditions exhibits self-referencing properties. An over ten-fold suppression of the effect caused by the variation of the bulk refractive index was observed. Y1 - 2015 U6 - https://doi.org/10.1016/j.snb.2014.10.022 SN - 0925-4005 VL - 207 IS - A SP - 740 EP - 747 ER - TY - GEN A1 - Klemm, Franziska A1 - Johnson, Richard A1 - Mirsky, Vladimir M. T1 - Binding of protein nanoparticles to immobilized receptors T2 - Sensors and Actuators B: Chemical N2 - The interaction of nanoparticles manufactured from human serum albumin (HSA) with different types of receptor surfaces was studied. Four different immobilization techniques were used to chemically link the receptors to the detector surfaces, namely: (i) a surface coated by monoclonal antibodies by a single step immobilization technique using carbodiimide, (ii) a surface coated by the same technique but using polyclonal antibodies, (iii) a surface coated by monoclonal antibodies immobilized through the sugar groups of antibodies and (iv) a surface coated by polyclonal antibodies immobilized by the same technique as in (iii). The results showed that there was almost no difference in the affinity of HSA in the molecular form to all of these surfaces. However, the binding behaviour of nanoparticles made from the same protein was quite different as successful nanoparticle binding was only observed when the surfaces were produced using method (ii). Y1 - 2015 U6 - https://doi.org/10.1016/j.snb.2014.10.135 SN - 0925-4005 VL - 208 SP - 616 EP - 621 ER - TY - PAT A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. A1 - Riepl, Michael T1 - Method for producing laterally organized structures on supporting surfaces Y1 - 2002 ER - TY - PAT A1 - Ando, Masanori A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Ozone detecting material and method for detecting ozone Y1 - 2002 ER - TY - PAT A1 - Mirsky, Vladimir M. A1 - Lange, Ulrich T1 - Multielectrode chemoresistor Y1 - 2010 ER - TY - GEN A1 - Drexler, Christoph A1 - Shishkanova, Tatiana V. A1 - Lange, Christoph A1 - Danilov, Sergey N. A1 - Weiss, Dieter A1 - Ganichev, Sergey D. A1 - Mirsky, Vladimir M. T1 - Terahertz split-ring metamaterials as transducers for chemical sensors based on conducting polymers: a feasibility study with sensing of acidic and basic gases using polyaniline chemosensitive layer T2 - Microchimica Acta N2 - We report on the first application of terahertz metamaterials acting as transducers for chemical sensors based on conducting polymers. In our feasibility study aimed at sensing of gaseous hydrochloric and ammonia, a two-dimensional sensor metamaterial consisting of an array of split-ring resonators on the surface of undoped silicon wafer was prepared. The surface of the resonator was coated with a 150-μm layer of polyaniline. Binding of hydrogen chloride to polyaniline leads to distinct changes in the resonance frequency of the metamaterial. Measurements can be performed both in the reflection and transmission mode. A numerical simulation of the response revealed an increase of both the real and the imaginary components of the dielectric function of the polyaniline film. These changes are attributed to the transition from emaraldine base to emeraldine salt. The results demonstrate a new approach for formation of highly sensitive transducers for chemical sensors. Y1 - 2014 U6 - https://doi.org/10.5283/epub.31493 SN - 1436-5073 VL - 181 SP - 1857 EP - 1862 ER - TY - GEN A1 - Barig, Susann A1 - Schiemann, Manja A1 - Mirsky, Vladimir M. A1 - Stahmann, Klaus-Peter T1 - Quantitative turbidity assay for lipolytic enzymes in microtiter plates T2 - Analytical and Bioanalytical Chemistry N2 - A clearing assay for lipolytic enzymes has been realized in 96-well microtiter plates. A thin layer containing emulsified tributyrin as turbidity-generating substrate was placed on a thicker supporting aqueous layer. Both layers were stabilized by a gel-forming agent. Enzyme addition leads to clearing of the emulsion detected with a standard microtiter plate reader as a decrease of extinction. Dependencies of the signal kinetics on the substrate and enzyme concentrations were studied. For 0.5–1 % tributyrin content the reaction rate is not substrate-limited. An initial slope of the signal kinetics is proportional to the lipase activity. A detailed characterization of the assay was performed. Lipolysis of tributyrin was confirmed by glycerol detection. Various gel-forming agents were compared and diffusion conditions in these gels were analyzed. Agar and agarose were found to be the most suitable gel-forming agents, which do not affect enzyme diffusion whereas polyacrylamide gels block lipase diffusion and therefore are not suitable for the assay. The optimized assay prepared from 1 % tributyrin emulsion in 2 % agar gel was tested with six microbial lipases and porcine pancreatic lipase. The detection limit is 20–60 ng/well which is equivalent to 30 μU/well for T. lanuginosus lipase. KW - Lipase assay KW - Lipolytic enzymes KW - Microtiter plates KW - Turbidity KW - Tributyrin Y1 - 2013 U6 - https://doi.org/10.1007/s00216-013-7283-5 SN - 1618-2650 VL - 405 IS - 26 SP - 8539 EP - 8547 ER - TY - GEN A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. T1 - Electroanalytical measurements without electrolytes: Conducting polymers as probes for redox titration in non-conductive organic media T2 - Analytica Chimica Acta N2 - Electroanalytical methods have been applied only in conducting media. An application of conducting polymers allows to overcome this limitation. If such material is in electrochemical equilibrium with dissolved redox active species, its electrical conductivity depends on the redox potential of these species. Therefore, conductometric measurements with conducting polymers can provide about the same information as classical redox electrodes. The approach was applied for redox titration. Equivalent points obtained by this titration in aqueous and organic electrolytes were identical. Then the approach was applied for determination of bromine number by redox titration in non-conducting organic phase. KW - Conducting polymers KW - Redox titration KW - Conductometric sensors KW - Non-conductive media Y1 - 2012 U6 - https://doi.org/10.1016/j.aca.2012.07.024 SN - 0003-2670 VL - 744 SP - 29 EP - 32 ER - TY - GEN A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. T1 - Polythiophene films on gold electrodes: A comparison of bulk and contact resistances in aqueous and organic media T2 - Journal of Solid State Electrochemistry N2 - Recently, developed technique for separated analysis of bulk and contact resistance was applied for the investigation of polythiophene films electropolymerized in boron trifluoride diethylether. Kinetics of polymer resistance and for the first time of the contact resistance during polymer oxidation and reduction were characterized. Influence of electrochemically controlled oxidation state on the polymer bulk and the polymer/metal contact resistance was measured in aqueous and organic environment. Variation of the electrical potential from −0.2 to 1.1 V vs. Ag/AgCl (sat) leads to an increase of the polymer conductivity for about three orders of magnitude and to a decrease of the contact resistance for about three orders of magnitude. The potential dependence of the two resistances was different, especially at high anodic potentials. In organic solution, the change of both resistances was more than six orders of magnitude. The results were compared with electrochemical and spectroelectrochemical data, a difference in the material behavior depending on the electrolyte solvent was observed. The influence of electrical potential on polymer resistance in aqueous solution was explained quantitatively by a three-state model with the values of oxidation potential +0.3 and +1.2 V. KW - Polythiophene KW - In situ conductivity measurement KW - s24-Technique KW - Spectroelectrochemistry KW - Conducting polymers Y1 - 2011 U6 - https://doi.org/10.1007/s10008-011-1450-4 SN - 1433-0768 VL - 15 IS - 11 SP - 2377 EP - 2382 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - Self-referencing SPR-biosensors based on penetration difference of evanescent waves T2 - Biosensors and Bioelectronics N2 - SPR based biosensors register binding of analytes to the surface with immobilized receptors by measuring changes of the refractive index near this surface. An important task in the improvement of this measurement technology is a separation of signals, corresponding to the changes in the chemosensitive layer, from undesired contributions of bulk phase, for example, due to fluctuations of temperature, concentrations of solutes, pressure. The wavelength of the incident light influences strongly the penetration depth of the corresponding evanescent wave. This dependence was exploited here for compensation of the contribution of the bulk refractive index. It was performed using differential SPR measurements at two wavelengths with differing penetration depths. Theoretical analysis and numerical optimization of the suggested approach, named a Penetration Difference Self-Referencing SPR (PDSR-SPR), were performed. Experimental test was performed using 658 and 980 nm laser diodes. Over 20 times suppression of variations of bulk refractive index with magnitude up to 1000 μRIU was observed. Finally, PDSR-SPR approach was applied for monitoring of antibodies binding to the immobilized antigens. KW - Surface plasmon resonance KW - SPR-biosenso KW - Evanescent wave KW - Affinity senso KW - Immunosensor Y1 - 2011 U6 - https://doi.org/10.1016/j.bios.2011.07.030 SN - 0956-5663 VL - 28 IS - 1 SP - 263 EP - 269 ER - TY - GEN A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. T1 - Integrated electrochemical transistor as a fast recoverable gas sensor T2 - Analytica Chimica Acta N2 - A new design of conductometric chemical sensors based on conducting polymers as chemosensitive elements was suggested. The sensor includes six electrodes. Four inner electrodes coated by chemosensitive polymer are used for simultaneous two- and four-point resistance measurements thus providing information on the bulk polymer resistance and on the resistance of the polymer/electrode contacts. Two outer electrodes wired to inner electrodes by polymeric electrolyte are used for electrical control of redox state of the chemosensitive polymer. The outer electrodes are connected to potentiostat as reference and counter electrodes. It allows us to control redox state of the inner (working) electrodes. This new measurement configuration, resembling chemosensitive electrochemical transistors, provides an internal test of the sensor integrity and an electrically driven sensor regeneration. It was tested as a sensor for the detection of nitrogen dioxide. Polythiophene or polyaniline was used as receptors. Cyclic voltammograms of these polymers on the sensor surface measured in air atmosphere were very similar to that measured in aqueous electrolyte. A control of conductivity of these chemosensitive polymers by electrical potential applied vs. incorporated reference electrode was demonstrated. This effect was used for the regeneration of the chemosensitive material after exposure to nitrogen dioxide: in comparison to usual chemiresistors displaying an irreversible behavior in such test even in the time scale of hours, a completely reversible sensor regeneration within few minutes was observed. KW - Gas sensor KW - Conducting polyme KW - Electrochemical transistor KW - Six-point resistance Measurement KW - Sensor recovery Y1 - 2011 U6 - https://doi.org/10.1016/j.aca.2010.12.008 SN - 0003-2670 VL - 687 IS - 1 SP - 7 EP - 11 ER - TY - GEN A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. T1 - Chemiresistors based on conducting polymers: A review on measurement techniques T2 - Analytica Chimica Acta N2 - This review covers the development of measurement configurations for chemiresistors based on conducting polymers. The simplest chemiresistors are based on application of a two-electrode technique. Artifacts caused by contact resistance can be overcome by application of a four-electrode technique. Simultaneous application of the two- and four-electrode measurement configurations provides an internal control of sensor integrity. An incorporation of two additional electrodes controlling the redox state of chemosensitive polymers and connecting to the measurement electrodes through liquid or (quasi)solid electrolyte results in a six-electrode technique; an electrically driven regeneration of such sensors allows one to perform fast and completely reversible measurements. KW - Chemiresistor KW - Chemotransistor KW - Conducting polymer KW - Chemosensor KW - Contact resistance KW - s24-Technique Y1 - 2011 U6 - https://doi.org/10.1016/j.aca.2010.11.030 SN - 0003-2670 VL - 687 IS - 2 SP - 105 EP - 113 ER - TY - GEN A1 - Lange, Ulrich A1 - Hirsch, Thomas A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Hydrogen sensor based on graphene - palladium nanocomposite T2 - Electrochimica Acta N2 - A composite material was prepared from graphene and palladium nanoparticles (PdNP) by layer-by-layer deposition on gold electrodes. The material was characterized by absorption spectroscopy, scanning electron microscopy, Raman spectroscopy and surface plasmon resonance. Cyclic voltammetry demonstrated the presence of electrocatalytic centers in the palladium decorated graphene. This material can serve as a sensor material for hydrogen at levels from 0.5 to 1% in synthetic air. Pure graphene is poorly sensitive to hydrogen, but incorporation of PdNPs increases its sensitivity by more than an order of magnitude. The effects of hydrogen, nitrogen dioxide and humidity were studied. Sensor regeneration is accelerated in humid air. The sensitivity of the nanocomposite depends on the number of bilayers of graphene–PdNPs. KW - Graphene KW - Palladium KW - Nanoparticle KW - Sensor KW - Layer-by-layer Y1 - 2011 U6 - https://doi.org/10.1016/j.electacta.2010.10.078 SN - 0013-4686 VL - 56 IS - 10 SP - 3707 EP - 3712 ER - TY - GEN A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. T1 - Chemosensitive nanocomposite for conductometric detection of hydrazine and NADH T2 - Electrochimica Acta N2 - A new chemosensitive material based on palladium nanoparticles and PEDOT-PSS is described. The composite was characterized by transmission electron microscopy, cyclic voltammetry and in situ resistance measurements. The material was applied for conductometric detection of hydrazine and NADH. Upon exposure to these analytes PEDOT is reduced leading to an increase in its conductance. This process is catalyzed by palladium. A model for description of the potential dependence of polymer conductivity was suggested, tested and applied for the development of new calibration procedure of chemiresistors based on electroactive polymers. KW - Chemosensor KW - Chemiresistor KW - Nanoparticles KW - Palladium KW - PEDOT KW - Hydrazine KW - NADH Y1 - 2011 U6 - https://doi.org/10.1016/j.electacta.2010.08.092 SN - 0013-4686 VL - 56 IS - 10 SP - 3679 EP - 3684 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Detection and quantification of single engineered nanoparticles in complex samples using template matching in wide-field surface plasmon microscopy T2 - Analytical Chemistry N2 - An ultrasensitive analytical method for direct detection of single nanoparticles in complex environment is described. The method relies on the wide-field surface plasmon microscopy (SPM). The suppression of matrix effects is achieved by image analysis based on the template matching. First, characteristic SPM images of nanoparticles are collected in aqueous suspensions. Then the detection of nanoparticles in complex environment is performed using template matching. Quantification and characterization of nanoparticles size was demonstrated at subppb level (∼ 100 pg/mL) in such complex media as wines, fruit juices, or cosmetic formulation. Visualization of the nanoparticles is performed in real time. The method does not require any sample pretreatment. If the minimally acceptable adsorption rate is defined as one nanoparticle to the whole sensor surface per few seconds, the working range of the method is ∼ 10 6 to 10 10 nanoparticles per mL. Y1 - 2016 U6 - https://doi.org/10.1021/acs.analchem.6b02878 SN - 1520-6882 VL - 88 IS - 20 SP - 10206 EP - 10214 ER - TY - CHAP A1 - Yatsimirsky, Anatoly K. A1 - Mirsky, Vladimir M. ED - Mirsky, Vladimir M. ED - Yatsimirsky, Anatoly K. T1 - Quantitative affinity data on selected artificial receptors T2 - Articicial receptors for chemical sensors KW - affinity data KW - artificial receptors KW - molecularly imprinted polymers KW - receptor–analyte binding constants KW - spreader-bar systems Y1 - 2011 SN - 978-3-527-32357-9 U6 - https://doi.org/10.1002/9783527632480 SP - 441 EP - 462 PB - Wiley VCH Verlag CY - Weinheim ER - TY - CHAP A1 - Mirsky, Vladimir M. ED - Mirsky, Vladimir M. ED - Yatsimirsky, Anatoly K. T1 - Quantitative characterization of affinity properties of immobilized receptors T2 - Artificial Receptors for Chemical Sensors KW - adsorption isotherms KW - affinity properties KW - binding isotherm KW - Frumkin isotherm KW - immobilized receptors KW - Langmuir isotherm KW - quantitative characterization KW - sensors Y1 - 2011 SN - 978-3-527-32357-9 U6 - https://doi.org/10.1002/9783527632480.ch1 SP - 1 EP - 15 PB - Wiley-VCH Verlag CY - Weinheim ER - TY - CHAP A1 - Lange, Ulrich A1 - Roznyatovskaya, Nataliya V. A1 - Hao, Qingli A1 - Mirsky, Vladimir M. ED - Mirsky, Vladimir M. ED - Yatsimirsky, Anatoly K. T1 - Conducting polymers as artificial receptors in chemical sensors T2 - Artificial receptors for chemical sensors KW - artificial receptors polyaniline KW - chemosensors KW - conducting polymers KW - polyaniline KW - transducers Y1 - 2011 SN - 978-3-527-32357-9 U6 - https://doi.org/10.1002/9783527632480.ch12 SP - 363 EP - 392 PB - Wiley-VCH CY - Weinheim ER - TY - GEN A1 - Ivanov, Svetlozar D. A1 - Lange, Ulrich A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Electrocatalytically active nanocomposite from palladium nanoparticles and polyaniline: Oxidation of hydrazine T2 - Sensors and Actuators B: Chemical N2 - The layer by layer (LbL) adsorption technique was used to deposit a new electrocatalytic material consisting of palladium nanoparticles (Pd NPs) and polyaniline (PANI). As far as PANI adsorption did not affect the reactivity of the Pd NPs attached in the former adsorption step, the LbL technique offered the way of increasing the reactive Pd surface within a three-dimensional nanocomposite structure. In situ conductance measurements have shown that depending on the concentration of the PANI solution, used for the LbL adsorption, composites with either PANI-like (dependent on potential and pH) or metal-like (non-dependent on potential and pH) conductive behaviour can be obtained. Metal-like Pd NPs–PANI nanocomposites were studied as electrocatalytic materials for hydrazine oxidation. A linear concentration dependence of the voltammetric peak currents was observed in the 40–800 μM hydrazine concentration range, the sensitivity increasing with the amount of adsorbed Pd NPs. Amperometric measurements showed linear response in the 10–300 μM range with sensitivity 0.5 μA/μmol cm−2 and a theoretical detection limit estimated to be 0.06 μM. KW - Conducting polymers KW - Pd-nanoparticles KW - LbL adsorption KW - Hydrazine Y1 - 2010 U6 - https://doi.org/10.1016/j.snb.2010.07.004 SN - 0925-4005 VL - 150 IS - 1 SP - 271 EP - 278 ER - TY - GEN A1 - Fassold, Alexander A1 - Falk, Werner A1 - Anders, Sven A1 - Hirsch, Thomas A1 - Mirsky, Vladimir M. A1 - Straub, Rainer H. T1 - Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis T2 - Arthritis and Rheumatology N2 - In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of this study was to investigate the role of neuropilin-2 in the synovial tissue of patients with RA and patients with osteoarthritis (OA) and in experimental arthritis. The density of neuropilin-2–positive fibers and cells positive for semaphorin 3F (a sympathetic repellent) was investigated using immunofluorescence staining. Enzyme-linked immunosorbent assay was used to detect soluble neuropilin-2 in body fluids from patients with RA and patients with OA. An axon outgrowth assay and a neuropilin-2 Fc fusion construct (neuropilin-2Fc) were used to investigate semaphorin 3F–induced sympathetic nerve repulsion. In an animal model of type II collagen–induced arthritis, soluble neuropilin-2Fc was studied in vivo. The synovial density of neuropilin-2–positive sympathetic nerve fibers was lower in RA than in OA, but the density of cells positive for semaphorin 3F was similar. In synovial fluid, the level of soluble neuropilin-2 was markedly higher in RA compared with OA. Mouse sympathetic ganglia served as an excellent model with which to study semaphorin 3F–induced nerve fiber repulsion. Neuropilin-2 and its coreceptor were present on sympathetic neurons, and semaphorin 3F bound to neuropilin-2Fc (binding constant 96 nmoles/liter). Semaphorin 3F dose-dependently increased sympathetic nerve fiber repulsion (at a 50% maximum response concentration of 160–210 nmoles/liter). In contrast to our expectations, soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. In experimental arthritis, therapy with neuropilin-2Fc aggravated arthritis. Soluble neuropilin-2 has no antirepellent activity but aggravates sympathetic nerve fiber repulsion and arthritis. Increased shedding of neuropilin-2 is probably an unfavorable sign in RA. Y1 - 2009 U6 - https://doi.org/10.1002/art.24860 SN - 2326-5205 VL - 60 IS - 10 SP - 2892 EP - 2901 ER - TY - GEN A1 - Shishkov, Alexander V. A1 - Bogacheva, Elena Nikolaevna A1 - Dolgov, Alexey A. A1 - Chulikov, Alexey L. A1 - Knyazev, Denis G. A1 - Fedorova, Natalia V. A1 - Ksenofontov, Alexander L. A1 - Kordyukova, Larisa V. A1 - Lukashina, Elena V. A1 - Mirsky, Vladimir M. A1 - Baratova, Lyudmila A. T1 - The in situ structural characterization of the influenza A virus matrix M1 protein within a virion T2 - Protein and Peptide Letters N2 - The first attempt has been made to suggest a model of influenza A virus matrix M1 protein spatial structure and molecule orientation within a virion on the basis of tritium planigraphy data and theoretical prediction results. Limited in situ proteolysis of the intact virions with bromelain and surface plasmon resonance spectroscopy study of the M1 protein interaction with lipid coated surfaces were used for independent confirmation of the proposed model. KW - Influenza A virus KW - matrix M1 protein KW - secondary structure prediction KW - tritium planigraphy KW - surface plasmon resonance spectroscopy KW - three-dimensional modeling Y1 - 2009 U6 - https://doi.org/10.2174/092986609789353682 SN - 0929-8665 SN - 1875-5305 VL - 16 IS - 11 SP - 1407 EP - 1413 ER - TY - CHAP A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. ED - Hashmi, S. A. ED - Chandra, A. T1 - Potential and pH-dependencies of bulk and contact resistances of conducting polymers T2 - Electroactive polymers: Materials and devices, Vol. 3 Y1 - 2009 SP - 37 EP - 46 PB - Macmillan Publisher CY - New Delhi ER - TY - GEN A1 - Wolpert, Benjamin A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Gas sensing properties of electrically conductive Cu(I) compounds at elevated temperatures T2 - Sensors and Actuators B: Chemical N2 - Changes in the electrical conductance at 240 °C of a number of copper(I) compounds including CuI, CuBr, Cu2Te, Cu12Sb4S13, (CuI)2P14, and of NiO were measured following the exposure to the gases ammonia, nitrogen dioxide, nitrous oxide, carbon monoxide, carbon dioxide, and propane. Analytical sensitivity and kinetic parameters were compared quantitatively. The high diversity of sensitivity patterns makes these compounds promising candidates for use in sensor arrays. Principal component analysis of the data obtained with a virtual sensor array enabled highly selective sensing of five of the six analytes studied using only two principle components. Activation energies of the charge transport in the materials were determined. KW - Gas sensor KW - Ammonia sensor KW - Conductometric sensor KW - Sensor array KW - Copper(I) KW - High-temperature sensing Y1 - 2009 U6 - https://doi.org/10.1016/j.snb.2009.04.042 SN - 0925-4005 VL - 142 IS - 2 SP - 446 EP - 450 ER - TY - GEN A1 - Ivanov, Svetlozar D. A1 - Kurniawan, Fredy A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Automated layer-by-layer deposition of polyelectrolytes in flow mode T2 - Macromolecular Materials and Engineering N2 - Multilayer structures of conducting polymers were fabricated by a simply automated approach in flow mode. Polyaniline and poly(styrene sulfonate) were used as a model system, allowing a fast electrochemical and spectroscopic determination of the amount of deposited material. The technology was applied for layer-by-layer deposition of up to 100 bilayers. The results demonstrate a well reproducible and almost constant amount of the adsorbed polymer at each deposition cycle. The method can be applied for deposition of other conducting or non-conducting polymers, biological macromolecules and composites of polyelectrolytes and nanoparticles. Y1 - 2009 U6 - https://doi.org/10.1002/mame.200800376 SN - 1439-2054 VL - 294 IS - 6-7 SP - 441 EP - 444 ER - TY - CHAP A1 - Potyrailo, Radislav A. A1 - Mirsky, Vladimir M. ED - Ryan, Margaret A. ED - Shevade, Abhijit V. ED - Taylor, Charles J. ED - Homer, M. L. ED - Blanco, Mario ED - Stetter, Joseph R. T1 - Development of new sensing materials using combinatorial and high-throughput experimentation T2 - Computational Methods for Sensor Material Selection N2 - New sensors with improved performance characteristics are needed for applications as diverse as bedside continuous monitoring, tracking of environmental pollutants, monitoring of food and water quality, monitoring of chemical processes, and safety in industrial, consumer, and automotive settings. Typical requirements in sensor improvement are selectivity, long-term stability, sensitivity, response time, reversibility, and reproducibility. Design of new sensing materials is the important cornerstone in the effort to develop new sensors. Often, sensing materials are too complex to predict their performance quantitatively in the design stage. Thus,combinatorial and high-throughput experimentation methodologies provide an opportunity to generate new required data to discover new sensing materials and/or to optimize existing material compositions. The goal of this chapter is to provide an overview of the key concepts of experimental development of sensing materials using combinatorial and high-throughput experimentation tools, and to promote additional fruitful interactions between computational scientists and experimentalists. Y1 - 2009 U6 - https://doi.org/10.1007/978-0-387-73715-7_7 SP - 151 EP - 166 PB - Springer CY - New York, NY [u.a.] ER - TY - GEN A1 - Wolpert, Benjamin A1 - Leitl, Michael A1 - Pfitzner, Arno A1 - Mirsky, Vladimir M. T1 - Chemosensitive properties of electrically conductive Cu(I)-compounds at room temperature T2 - Sensors and Actuators B: Chemical N2 - Changes of the electrical conductance of a number of inorganic copper(I) conductive compounds including Cu₁₂Sb₄S₁₃,CU₂O, CuI, CuBr and CuCl were tested on exposure to different gases and vapors, including ammonia, nitrogen monoxide, octanethiol, hydrogen chloride, acetic acid, formic acid, ethanol, humidity and piperidine. The sensitivity of the resistivity to ammonia was estimated as 2.7%/ppm for CuBr,1.4%/ppm for Cu₂O, 1.2%/ppm for CuCl, 0.013%/ppm for CuI, but only 0.0028%/ppm for Cu₁₂Sb₄S₁₃. The highest selectivity for ammonia was observed for Cu₁₂Sb₄S₁₃. The sensitivity patterns of these materials to the tested gases and vapors are very different which makes them applicable for sensor arrays for identification and quantification of these species. KW - Chemical sensor KW - Gas sensor KW - Ammonia sensor KW - Conductometric sensor KW - Chemoresistor KW - Copper(I) Y1 - 2008 U6 - https://doi.org/10.1016/j.snb.2008.06.047 SN - 0925-4005 VL - 134 IS - 2 SP - 839 EP - 842 ER - TY - GEN A1 - Lange, Ulrich A1 - Mirsky, Vladimir M. T1 - Separated analysis of bulk and contact resistance of conducting polymers: comparison of simultaneous 2- and 4-point measurements with impedance measurements T2 - Journal of Electroanalytical Chemistry N2 - Simultaneous measurements of conductive polymer by two-and four-point techniques were used for evaluation of the contact resistance for the polymer/metal interface. An experimental validation of this approach was performed for polypyrrole electrochemically deposited on gold electrodes. The dependence of contact resistance on the electrode potential versus reference electrode was measured. The results were compared with the corresponding dependence obtained from impedance spectroscopy. Both techniques provide almost identical data while the new approach is easier, faster and independent on selection of equivalent circuits. KW - Conducting polymer KW - Polypyrrole KW - Contact resistance KW - Simultaneous two KW - Four-point measurements Y1 - 2008 U6 - https://doi.org/10.1016/j.jelechem.2008.06.013 SN - 1572-6657 VL - 622 IS - 2 SP - 246 EP - 251 ER - TY - GEN A1 - Hodzhaoglu, Feyzim A1 - Kurniawan, Fredy A1 - Mirsky, Vladimir M. A1 - Nanev, Christo T1 - Gold nanoparticles induce protein crystallization T2 - Crystal Research and Technology N2 - Nucleation of protein crystals by gold nanoparticles was observed. Lysozyme and ferritin were used as model proteins. The effect was established with uncoated gold nanoparticles and with gold nanoparticles coated by 16-mercaptodecanoic acid. KW - gold nanoparticles KW - protein crystallization KW - nucleants KW - lysozyme KW - ferritin Y1 - 2008 U6 - https://doi.org/10.1002/crat.200811125 SN - 1521-4079 VL - 43 IS - 6 SP - 588 EP - 593 ER - TY - GEN A1 - Lange, Ulrich A1 - Roznyatovskaya, Nataliya V. A1 - Mirsky, Vladimir M. T1 - Conducting polymers in chemical sensors and arrays (invited review) T2 - Analytica Chimica Acta N2 - The review covers main applications of conducting polymers in chemical sensors and biosensors. The first part is focused on intrinsic and induced receptor properties of conducting polymers, such as pH sensitivity, sensitivity to inorganic ions and organic molecules as well as sensitivity to gases. Induced receptor properties can be also formed by molecularly imprinted polymerization or by immobilization of biological receptors. Immobilization strategies are reviewed in the second part. The third part is focused on applications of conducting polymers as transducers and includes usual optical (fluorescence, SPR, etc.) and electrical (conductometric, amperometric, potentiometric, etc.) transducing techniques as well as organic chemosensitive semiconductor devices. An assembly of stable sensing structures requires strong binding of conducting polymers to solid supports. These aspects are discussed in the next part. Finally, an application of combinatorial synthesis and high-throughput analysis to the development and optimization of sensing materials is described. KW - Conducting polymers KW - Chemical sensors KW - Electroactive polymers KW - Gas sensors KW - Combinatorial techniques KW - Electropolymerization KW - Sensor array Y1 - 2008 U6 - https://doi.org/10.1016/j.aca.2008.02.068 SN - 0003-2670 VL - 614 IS - 1 SP - 1 EP - 26 ER - TY - GEN A1 - Kurniawan, Fredy A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Analytical applications of electrodes modified by gold nanoparticles: dopamine detection T2 - Journal of Nanoscience and Nanotechnology N2 - Dopamine oxidation was studied on modified gold (nano-Au) electrodes obtained by Layer-by-Layer deposition of gold nanoparticles and polyacrylic acid. A gradual loss of electrochemical activity for the dopamine oxidation reaction is observed at pH 7. Simultaneous SPR spectroscopy and cyclic voltammetry indicate the formation of an adsorbed layer on the electrode surface at this pH value. Investigations, performed through electrochemical and SPR measurements at pH 4, give evidence for a reversible process. At this pH value both dopamine oxidation and reduction current peaks show linear dependence on the dopamine concentration and may be used for analytical applications. The use of the nano-Au electrode allows resolving the peaks corresponding to ascorbic acid and to dopamine oxidation by 240 mV thus providing a high selectively for dopamine detection in the presence of ascorbic acid. The detection limit of this electrode for dopamine is below 4 microM in the presence of 1 mM ascorbic acid. The sensitivity normalized to the macroscopic electrode surface is about 10 mA cm(-2) mM(-1) at sweep rate of 10 V/s. KW - ascorbic acid KW - dopamine KW - electrochemical detection KW - gold nanoparticles KW - surface plasmon resonance Y1 - 2009 U6 - https://doi.org/10.1166/jnn.2009.SE13 SN - 1533-4880 SN - 1533-4899 VL - 9 IS - 4 SP - 2407 EP - 2412 ER - TY - GEN A1 - Boecker, Daniel A1 - Zybin, Alexander A1 - Niemax, Kay A1 - Grunwald, Christian A1 - Mirsky, Vladimir M. T1 - Noise reduction by multiple referencing in surface plasmon resonance imaging T2 - Review of Scientific Instruments N2 - The analytical performance of surface plasmon resonance imaging with charge coupled device detection can be improved significantly by splitting a macroscopic sensing surface into multiple microscopic neighboring sensing and referencing subareas. It is shown that such a multiple referencing reduces intensity fluctuations across the total sensing area and, therefore, improves the signal/noise (S/N)ratio proportional to the splitting factor. The approach is demonstrated by detection of biotin binding to a monolayer of streptavidin. An effective variation of the reflected intensity of about 10⁻⁴, which corresponds to the refraction index variation of 3 x 10⁻⁶, was detected with a S/N ratio about 10 without any temperature stabilization of the sensing area. Y1 - 2008 U6 - https://doi.org/10.1063/1.2888527 SN - 1527-2400 VL - 79 IS - 2 SP - 023110 ER - TY - CHAP A1 - Potyrailo, Radislav A. A1 - Mirsky, Vladimir M. ED - Potyrailo, Radislav A. ED - Mirsky, Vladimir M. T1 - Combinatorial methods for chemical and biological sensors: Outlook T2 - Combinatorial Methods for Chemical and Biological Sensors N2 - This chapter provides a summary of status of combinatorial development of materials for chemical and biological sensors and an outlook for the future developments. Y1 - 2009 SN - 978-0-387-73712-6 U6 - https://doi.org/10.1007/978-0-387-73713-3_21 SP - 483 EP - 489 PB - Springer CY - New York, NY [u.a.] ER - TY - CHAP A1 - Potyrailo, Radislav A. A1 - Mirsky, Vladimir M. ED - Potyrailo, Radislav A. ED - Mirsky, Vladimir M. T1 - Introduction to combinatorial methods for chemical and biological sensors T2 - Combinatorial Methods for Chemical and Biological Sensors N2 - Sensing materials play a critical role in advancing selectivity, response speed, and sensitivity of chemical and biological determinations in gases and liquids. The desirable capabilities of sensing materials originate from their numerous functional parameters, which can be tailored to meet specific sensing needs. By increasing the structural and functional complexity of sensing materials, the ability to rationally define the precise requirements that will result in desired materials properties becomes increasingly limited. Combinatorial experimentation methodologies impact all areas of sensing materials research including inorganic, organic, and biological sensing materials. Y1 - 2009 SN - 978-0-387-73712-6 U6 - https://doi.org/10.1007/978-0-387-73713-3_1 SP - 3 EP - 24 PB - Springer CY - New York, NY [u.a.] ER - TY - CHAP A1 - Mirsky, Vladimir M. ED - Potyrailo, Radislav A. ED - Mirsky, Vladimir M. T1 - Combinatorial development of chemosensitive conductive polymers T2 - Combinatorial Methods for Chemical and Biological Sensors N2 - Conductive polymers are established materials for development of chemical and biological sensors. Properties of these polymers are influenced by a number of different physical and chemical factors. Application of combinatorial and high-throughput techniques to development and optimization of chemo and biosensors is reviewed. Methods for addressable synthesis of conductive polymers and protocols for comprehensive description of chemosensitive properties are discussed. Y1 - 2009 SN - 978-0-387-73712-6 U6 - https://doi.org/10.1007/978-0-387-73713-3_13 SP - 315 EP - 330 PB - Springer CY - New York, NY [u.a.] ER - TY - GEN A1 - Potyrailo, Radislav A. A1 - Mirsky, Vladimir M. T1 - Combinatorial and high-throughput development of sensing materials: The first ten years T2 - Chemical Reviews Y1 - 2008 U6 - https://doi.org/10.1021/cr068127f SN - 0009-2665 SN - 1520-6890 VL - 108 SP - 770 EP - 813 ER - TY - GEN A1 - Zybin, Alexander A1 - Boecker, Daniel A1 - Mirsky, Vladimir M. A1 - Niemax, Kay T1 - Enhancement of the detection power of surface plasmon resonance measurements by optimization of the reflection angle T2 - Analytical Chemistry N2 - The detection limit of surface plasmon resonance (SPR) measurements has been improved by a factor of approximately 2-3.5 if the angle of incidence was near to the reflection minimum of the SPR resonance curve instead at the position of the steepest slope, the standard alignment in SPR imaging. The enhancement of the detection power, a result of signal-to-noise optimization, is demonstrated by applying a photodiode and a CCD camera for SPR detection. The experimental data are compared with data expected from theory. Y1 - 2007 U6 - https://doi.org/10.1021/ac070074u SN - 0003-2700 SN - 1520-6882 VL - 79 IS - 11 SP - 4233 EP - 4236 ER - TY - GEN A1 - Delaney, Tetyana L. A1 - Zimin, Denys A1 - Rahm, Michael A1 - Weiss, Dieter A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Capacitive detection in ultrathin chemosensors prepared by molecularly imprinted grafting photopolymerization T2 - Analytical Chemistry N2 - The usual applications of capacitive detection in chemo- and biosensors are based on changes in effective thickness of insulating layers due to adsorption of analyte onto receptors. Ultrathin chemosensors based on molecularly imprinted polymerization enable a realization of another capacitive approach that exploits changes in electrical capacitance due to modification of the dielectric constant of the polymer. Such chemosensors were prepared by photografted molecularly imprinted polymerization on the surface of gold electrodes. An adsorbed layer of hydrophobic photoinitiator (benzophenone) provided grafted polymerization on the surface of the alkanethiol-modified gold electrode. The chemosensors were characterized by cyclic voltammetry, impedance spectroscopy, and scanning electron and atomic force microscopy. Binding of analyte was detected by measurements of electrical capacitance. The results indicate a decrease of the dielectric constant of the polymer layer due to analyte binding up to 20%. Y1 - 2007 U6 - https://doi.org/10.1021/ac062143v SN - 0003-2700 SN - 1520-6882 VL - 79 IS - 8 SP - 3220 EP - 3225 ER - TY - CHAP A1 - Mirsky, Vladimir M. ED - Corain, Benedetto ED - Schmid, Günter ED - Toshima, Naoki T1 - Spreader-bar structures as molecular templates for electrochemical synthesis of nanoparticles T2 - Metal nanoclusters in catalysis and materials science: the issue of size-control Y1 - 2008 U6 - https://doi.org/10.1016/B978-044453057-8.50020-9 SP - 321 EP - 325 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Hirsch, Thomas A1 - Shaporenko, Andrey A1 - Mirsky, Vladimir M. A1 - Zharnikov, Michael T1 - Monomolecular films of phthalocyanines: formation, characterisation and expelling by alkanethiols T2 - Langmuir N2 - Adsorption of aluminum-2,3-naphthalocyanine (Al-PC) onto gold (111) substrate from the pure and mixed (with alkanethiols) solutions of the target molecules in ethanol was studied. The resulting films were characterized by X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure spectroscopy. The adsorption from the pure solution resulted in formation of an Al-PC monolayer composed of the strongly inclined molecules. However, a presence of only one molar percent of a thiolated compound (alkanethiol) in the primary solution led to the complete expelling of the Al-PC molecules from the substrate and formation of a one-component alkylthiolate monolayer. The results indicate that an equilibrium formation of mixed monolayers of rodlike thiolated molecules and relatively large planar-geometry molecules, whose interaction with the metal surface is mainly provided by the π-electron system, is difficult to achieve or is in most cases even impossible under equilibrium conditions and requires an introduction of additional anchor moieties (e.g., thiols) into these molecules, as has been demonstrated by successful coadsorption of hexadecanethiol and thiolated tetraphenylporphyrin. Y1 - 2007 U6 - https://doi.org/10.1021/la0634239 SN - 0743-7463 SN - 1520-5827 VL - 23 IS - 8 SP - 4373 EP - 4377 ER - TY - GEN A1 - Cao, Limin A1 - Lin, Hong A1 - Mirsky, Vladimir M. T1 - DNA-based surface plasmon resonance biosensor for enrofloxacin T2 - Analytica Chimica Acta N2 - A DNA-based surface plasmon resonance biosensor for enrofloxacin was developed. Heating denatured DNA immobilized on the gold-coated glass surface was exploited. The immobilization was performed by a layer-by-layer co-deposition with a cationic polymer. The sensor performance was tested with real biological probes. Direct and simple determination of enrofloxacin in milk samples was demonstrated. The sensor response obeys Langmuir binding isotherm being almost linear until about 20 µg per mL. The detection limit in milk samples was estimated to be 3 µg per mL. KW - Surface plasmon resonance KW - Nucleic acid KW - Biosensor KW - Enrofloxacin KW - Fluoroquinolones KW - Layer-by-layer deposition Y1 - 2007 U6 - https://doi.org/10.1016/j.aca.2007.02.034 SN - 0003-2670 VL - 589 IS - 1 SP - 1 EP - 5 ER - TY - GEN A1 - Cao, Limin A1 - Lin, Hong A1 - Mirsky, Vladimir M. T1 - Detection of antibiotics in food: Extraction of fluoroquinolones by DNA T2 - Analytical and Bioanalytical Chemistry N2 - The ability of DNA to extract fluoroquinolones from model solutions and real probes of food was demonstrated and investigated quantitatively. The interaction between fluoroquinolones and different types of DNA was studied by equilibrium dialysis. The first application of this direct approach allowed us to determine binding constants and binding stoichiometries in different conditions. The binding of enrofloxacin to heat-denatured DNA (d-DNA) from herring sperm is pH- and magnesium-dependent; the highest fraction of bound drugs was found at pH 7 and a magnesium concentration of 0.5–1 mM. Results for three types of DNA: d-DNA, double-stranded DNA and single-stranded DNA were compared. The unwound DNA showed almost doubled binding constants and stoichiometries, thus indicating preferable interaction of enrofloxacin with single-strand regions of DNA. The binding of other fluoroquinolones (lomefloxacin, ciprofloxacin, norfloxacin, danofloxacin and sarafloxacin) with d-DNA is very similar to that of enrofloxacin: the binding constants are in the range from 0.94 × 105 to 2.40 × 105 M−1, and the stoichiometries range from 4.1 to 6.9 fluoroquinolone molecules per 100 DNA bases. The binding properties were quantitatively the same for extraction of fluoroquinolones from model aqueous solutions and from liquid food (milk). The results indicate the efficiency of DNA for selective extraction of fluoroquinolones from real samples for further analysis. This selective binding also allows us to consider DNA as a natural receptor for development of analytical techniques for fluoroquinolones. KW - Fluoroquinolone KW - DNAExtraction KW - Binding constant KW - Binding stoichiometry Y1 - 2007 U6 - https://doi.org/10.1007/s00216-007-1191-5 SN - 1618-2642 SN - 1618-2650 VL - 388 IS - 1 SP - 253 EP - 258 ER - TY - CHAP A1 - Vasjari, Majlinda A1 - Mirsky, Vladimir M. ED - Alegret, Salvador ED - Merkoci, Arben T1 - Chemoresistor for determination of mercury vapor T2 - Electrochemical Sensor Analysis N2 - This chapter presents a procedure to prepare and calibrate chemical sensor for mercury vapor and discusses application of the sensor for measurements of mercury vapor. The sensor is based on high affinity of gold to mercury and on chemoresistive properties of ultrathin gold layers: adsorption of mercury leads to increase in the surface resistance. However, this effect is not selective: similar changes are caused by adsorption of water vapor and sulfuric compounds. The use of monomolecular layer of alkylthiols as a filter excludes this interference completely. Sensor calibration is performed by thermoinjection of nanogram-amount of mercury quantitatively deposited by electrochemical reduction. It is found that the sensors display no sensitivity to such typical interferents of mercury chemoresistors based on uncoated gold as volatile thiols and water. Preliminary data demonstrate a possibility to measure Hg(0) even in aqueous solutions. Y1 - 2007 SN - 978-0-444-53053-0 U6 - https://doi.org/10.1016/S0166-526X(06)49058-9 SP - 105 EP - 109 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Mirsky, Vladimir M. A1 - Vasjari, Majlinda ED - Alegret, Salvador ED - Merkoci, Arben T1 - Chemical sensors for mercury vapour T2 - Electrochemical Sensor Analysis N2 - A number of analytical methods were developed for determination of elemental mercury. They include traditional analytical techniques, such as atomic adsorption spectroscopy (AAS), atomic fluorescence spectroscopy (AFS), and atomic emission spectroscopy (AES). Other analytical methods include neutron-activation analysis and laser photo-acoustic spectroscopy (detection limit about 5 μg/l). To increase sensitivity and selectivity of these methods, a preconcentration of mercury is widely performed. This can be realized by exploring high affinity of mercury to gold. Mercury and its volatile compounds can easily be adsorbed on gold at normal temperatures and can be released quantitatively upon heating. Hence, gold has been used as an adsorbent for the accumulation of mercury to a concentration sufficient for further spectroscopic analyses. The main disadvantage of mercury sensors based on bare gold layers is their poor selectivity and applications of such sensors for real probes and makes necessary a pre-treatment of probes. Mercury sensors based on thin gold layers require regular calibrations. In ideal cases, the calibration should be performed before each measurement. Therefore, a calibration technique should be compatible with design and concept of the mercury vapor sensor. Y1 - 2007 SN - 978-0-444-53053-0 U6 - https://doi.org/10.1016/S0166-526X(06)49012-7 SP - 235 EP - 251 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Roznyatovskaya, Nataliya V. A1 - Laurinavichyute, Veronika K. A1 - Tsirlina, Galina A. A1 - Mirsky, Vladimir M. T1 - Electrochemistry and catalytic behavior of immobilized binuclear complexes of copper(II) and nickel(II) with Robson type ligand T2 - Journal of Solid State Electrochemistry N2 - Various systems containing immobilized binuclear copper(II) and nickel(II) complexes with Robson type ligand ([M2L]Cl2) are studied and compared in relation to catalysis of hydrogen peroxide reduction. Molecular complexes adsorbed on mercury and gold, crystalline complexes immobilized in the carbon paste electrode, and complex species entrapped into polyphenol-modified gold electrodes are considered. Electrocatalysis is assumed to result from the formation of H2O2–[M2L]Cl2 adduct, not from mediating redox transformations. Possible geometry of the formed reaction layers supporting adduct formation is discussed. KW - Binuclear copper macrocyclic complex KW - Cyclic voltammetry KW - Adsorption KW - Carbon paste electrodes KW - Electroreduction Y1 - 2007 U6 - https://doi.org/10.1007/s10008-006-0235-7 SN - 1432-8488 SN - 1433-0768 VL - 11 IS - 7 SP - 981 EP - 992 ER - TY - GEN A1 - Hao, Qingli A1 - Wang, Xin A1 - Lu, Lude A1 - Yang, Xujie A1 - Mirsky, Vladimir M. T1 - Electropolymerized multilayer conducting polymers with response to gaseous hydrogen chloride T2 - Macromolecular Rapid Communications N2 - A step-by-step ‘all-electrochemical’ approach has been presented to develop a multilayer structure of conducting polymers for gas sensors. The integrated structure includes a sensitive layer (polyaniline, PANI) and a conductive bridge consisting of poly(3,4-ethylenedioxythiophene) (PEDOT). Good sensitivity, stability, and response of the multilayer material to gaseous HCl indicate a possible application of conductive polymers to provide a binding of sensitive elements in sensors or other fields. The conducting multilayer material, Au/pATP/PANI/PEDOT, was synthesized electrochemically. Y1 - 2005 U6 - https://doi.org/10.1002/marc.200500114 SN - 1521-3927 VL - 26 IS - 13 SP - 1099 EP - 1103 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Kulikov, Valentin A1 - Wolfbeis, Otto S. T1 - Complete system for combinatorial synthesis and functional investigation of conductive polymers T2 - American Chemical Society PMSE Preprints N2 - Complete system for combinatorial electrochemical synthesis and functional characterization of combinatorial libraries formed by different polymers and polymeric structures will be presented. Control of electrical potential of single electrode groups consisting of four electrodes (designed for 4-point measurements ) on the array comprising 96 such electrode groups provides addressable electrochemical polymer synthesis on the defined electrodes. After polymerization, electrical characteristics of polymers or/and polymer/electrode contacts and influence of potential analytes on these characteristic are measured. Final data analysis provides complete analytically relevant information on the investigated thin polymer layers or multilayer polymer structures: absolute and relative sensitivity, response time, reversibility, reproducibility, regeneration kinetics, linearity or binding constant. Examples of applications for the development of gas-sensitive (co)polymers and optimization of their sensitive properties and polymer/metal contacts will be presented. Y1 - 2005 VL - 93 SP - 1053 ER - TY - GEN A1 - Hirsch, Thomas A1 - Zharnikov, Michael A1 - Shaporenko, Andrey A1 - Stahl, Joachim A1 - Weiss, Dieter A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Size-controlled electrochemical synthesis of metallic nanoparticles on molecular templates T2 - Angewandte Chemie International Edition N2 - Reduction on the spot: Planarly adsorbed thiolated porphyrin molecules (purple) incorporated in an insulating alkanethiol monolayer (blue) function as nanoelectrodes for the local reduction of metals (gray). This approach was applied for the in situ synthesis of nanoparticles of a predetermined size. Y1 - 2005 U6 - https://doi.org/10.1002/anie.200500912 SN - 1521-3773 VL - 44 IS - 41 SP - 6775 EP - 6778 ER - TY - GEN A1 - Zybin, Alexander A1 - Grunwald, Christian A1 - Mirsky, Vladimir M. A1 - Kuhlmann, Jürgen A1 - Wolfbeis, Otto S. A1 - Niemax, Kay T1 - Double-wavelength technique for surface plasmon resonance measurements: basic concept and applications for single sensors and two-dimensional sensor arrays T2 - Analytical Chemistry N2 - A new technique for on-line monitoring of analyte binding to sensor surfaces by surface plasmon resonance (SPR) detection is described. It is based on differential measurements using two wavelengths provided by two diode lasers. The technique is as simple and robust as the conventional SPR detection measuring the reflected radiation at fixed incidence angle, but it has the advantage of being nonsensitive to variations of the resonance width and providing essentially higher signal/noise ratios. The paper presents the first four channel prototype system for parallel 2D-monitoring at four different spots. One channel is always used as a reference to compensate temperature fluctuations and nonspecific adsorptions. Calibration with sucrose solutions revealed an absolute sensitivity of Δn ∼ 5 × 10-6. The new technique is tested with a biotin−streptavidin binding and with hybridization/denaturation of DNA. Biotin binding to a streptavidin monolayer is detected with a signal/noise ratio of about 5, which demonstrates the high potential of the new technique for applications in drug discovery. Applications to gene analysis are tested with short oligonucleotides of the sequences used for genotyping human hepatitis C viruses. A selective response to complementary oligonucleotides is observed. The high reproducibility in subsequent cycles of hybridization/denaturation (by formamide or by heating) points out potential applications of the technique in medical diagnostics, food industry, genomics, and proteomics too. Y1 - 2005 U6 - https://doi.org/10.1021/ac048156v SN - 0003-2700 SN - 1520-6882 VL - 77 IS - 8 SP - 2393 EP - 2399 ER - TY - CHAP A1 - Mirsky, Vladimir M. A1 - Kulikov, Valentin A1 - Hao, Qingli A1 - Wolfbeis, Otto S. T1 - Application of combinatorial electropolymerization to the development of chemical Sensors T2 - Combinatorial and artificial intelligence methods in materials science II, symposium held December 1 - 4, 2003, Boston, Massachusetts, U.S.A. N2 - Combinatorial electropolymerization with electrical addressing was realized on the array of 96 electrode groups, each from four electrodes. The polymer synthesis was combined with subsequent high-throughput investigation of analytical properties of synthesized polymers. Two-and four-point techniques were applied simultaneously to measure electrical properties of synthesized polymers and contact resistances between electrodes and polymers and modifications of these parameters on addition of analyte. The system was used for development of sensitive materials for detection of gaseous hydrogen chloride. Y1 - 2004 SN - 1-55899-742-3 U6 - https://doi.org/10.1557/PROC-804-JJ4.1 SP - 111 EP - 116 PB - Materials Research Society CY - Warrendale, Pa. ER - TY - GEN A1 - Kurniawan, Fredy A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Gold nanoparticles in non-enzymatic electrochemical detection of sugars T2 - Electroanalysis N2 - A nonenzymatic electrochemical sensor for detection of sugars was prepared by layer-by-layer deposition of gold nanoparticles on thin gold electrodes. The deposition was optimized by using of surface plasmon resonance. Voltammetric investigation and impedance spectroscopy of the sensor was performed. Electrical currents caused by glucose on bare gold electrodes and on gold electrodes coated by immobilized gold nanoparticles were compared. The electrodes with nanoparticles display much higher current of glucose oxidation. The oxidation becomes blocked when the swept electrode potential exceeded +0.25 V, during the back scan an oxidation peak is observed again but at less positive potential. The magnitudes of these current peaks are linearly dependent on the glucose concentration; this dependence can be used as calibration for analytical applications. The limit of detection for glucose is below 0.5 mM, the sensitivity (normalized to the macroscopic electrode surface) is about 160 μA cm-2 mM-1. The sensor response is linear till at least 8 mM of glucose concentration. KW - Gold nanoparticles KW - Electrochemical KW - Glucose detection Y1 - 2006 U6 - https://doi.org/10.1002/elan.200603607 SN - 1521-4109 VL - 18 IS - 19-20 SP - 1937 EP - 1942 ER - TY - GEN A1 - Mirsky, Vladimir M. T1 - Neue Technologie angewandter Polymerforschung. Hundert mal schneller zu neuen Sensormaterialien T2 - Aktuelle Wochenschau : HighChem hautnah. Aktuelles aus der analytischen Chemie Y1 - 2005 VL - 48 SP - 98 EP - 99 ER - TY - GEN A1 - Ivanov, Svetlozar D. A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Conductometric transducing in electrocatalytical sensors: Detection of ascorbic acid T2 - Electrochemistry Communications N2 - Conductometric transducing is suggested for electrocatalytic chemical sensors based on conductive polymers. Lateral conductivity of the polymer layers was measured by two- and four-point techniques. The approach was applied for electroanalytical detection of ascorbic acid on the electrocatalytically active polyaniline and poly-ortho-methoxyaniline electrodes. Both polymers displayed a monotonous concentration dependence of their conductance in the analytically important sub-millimolar concentration range of ascorbic acid. The approach was tested in model solutions of ascorbic acid in buffer and applied for detection of ascorbic acid in orange juice. KW - Chemical sensor KW - Conductometric sensor KW - Ascorbic acid KW - Electrocatalytical sensor KW - Electroactive polymers KW - Polyaniline KW - Poly-ortho-methoxyaniline Y1 - 2006 U6 - https://doi.org/10.1016/j.elecom.2006.02.006 SN - 1388-2481 VL - 8 IS - 4 SP - 643 EP - 646 ER - TY - GEN A1 - Zhi, Zheng-liang A1 - Drazan, Viktor A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Electrocatalytic activity of DNA on electrodes as an indication of hybridization T2 - Bioelectrochemistry N2 - Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNAwith the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample. KW - DNA KW - DNA-sensor KW - Biosensor KW - Electrocatalysi KW - Hybridisation KW - Oligonucleotide KW - Gene analysis Y1 - 2006 U6 - https://doi.org/10.1016/j.bioelechem.2005.02.006 SN - 1567-5394 VL - 68 IS - 1 SP - 1 EP - 6 ER - TY - GEN A1 - Krondak, Martin A1 - Broncová, Gabriela A1 - Anikin, Sergey A1 - Merz, Andreas A1 - Mirsky, Vladimir M. T1 - Chemosensitive properties of poly-4,4'-dialkoxy2,2’-bipyrroles T2 - Journal of Solid State Electrochemistry N2 - Chemosensitive properties of 4,4′-dimethoxy-2,2′-bipyrrole and 4,4′-dibuthoxy-2,2′-bipyrrole were investigated. These new conductive polymers were polymerized by cyclic voltammetry on the platinum and gold interdigitated electrodes designed for 2- and 4-point measurement of lateral conductivity. Thus, obtained polymers demonstrated high sensitivity to HCl in the parts per million concentration range. Kinetics of the gaseous HCl influence on the ratios of conductivities measured by 2- and 4-point techniques was analyzed. Some response of the polymers conductance towards NH3, NO and oxygen was also observed, but with much lower sensitivity than towards HCl. In aqueous solutions, the polymer conductivity was pH dependent; in comparison with 4,4′-dibuthoxy-2,2′-bipyrrole, the conductivity range of the methoxy derivative was shifted to acidic range for about two pH units. A possibility to use the pH dependence for the development of enzymatic biosensors with pH transducing was demonstrated. KW - Conductive polymer KW - Sensor KW - Conductivity KW - HCI KW - Alkoxybipyrroles Y1 - 2006 U6 - https://doi.org/10.1007/s10008-005-0069-8 SN - 1432-8488 SN - 1433-0768 VL - 10 IS - 3 SP - 185 EP - 191 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Kulikov, Valentin A1 - Hao, Qingli A1 - Wolfbeis, Otto S. T1 - Multiparameter High Throughput Characterization of Combinatorial Chemical Microarrays of Chemosensitive Polymers T2 - Macromolecular Rapid Communications N2 - High throughput characterization of combinatorial libraries demands a compromise between completeness of the study and reasonable investigation time. This compromise can be found by the development of test protocols consisting of the minimal number of the most informative measurements. Multiparameter high throughput characterization of sensitive materials is described. The parameters include response time, regeneration time, reversibility, reproducibility, sensitivity, linearity. The approach was applied for characterization of conductometric gas-sensitive polymer materials. Chemical microarrays prepared by electrochemical synthesis of polymers on platinum electrodes. Y1 - 2004 U6 - https://doi.org/10.1002/marc.200300210 SN - 1521-3927 VL - 25 IS - 1 SP - 253 EP - 258 ER - TY - GEN A1 - Kulikov, Valentin A1 - Mirsky, Vladimir M. T1 - Equipment for combinatorial electrochemical polymerization and high-throughput investigation of electrical properties of the synthesized polymers T2 - Measurement Science and Technology N2 - The first automated system for combinatorial synthesis and high throughput investigation of electrical properties of conductive polymers is described. The equipment provides a polymerization of defined mixtures of monomers into a thin layer on the addressed electrodes of an electrode array followed by measurements of current–voltage characteristics. The electrodes have an interdigital configuration, specially designed for four-point measurements. A programmed addition of gases or liquids provides automated investigation of the influence of different substances on the synthesized polymers. The results of an experimental test of the system are presented. This equipment can be used for synthesis and electrical characterization of conductive polymers and multilayer polymer structures, for development of chemical sensors, organic electronic devices and other thin film functional structures. KW - elect ropolymerization KW - chemical sensors KW - biological sensors KW - organic semiconductors KW - combinatorial approach KW - high-throughput screening Y1 - 2004 U6 - https://doi.org/10.1088/0957-0233/15/1/007 SN - 1361-6501 SN - 0957-0233 VL - 15 IS - 1 SP - 49 EP - 54 ER - TY - CHAP A1 - Sokolov, Valeri S. A1 - Mirsky, Vladimir M. ED - Mirsky, Vladimir M. T1 - Electrostatic potentials of bilayer lipid membranes: basic principles and analytical applications T2 - Ultrathin Electrochemical Chemo- and Biosensors: Technology and Performance Y1 - 2004 SN - 978-3-642-05961-2 SN - 978-3-662-05204-4 SP - 255 EP - 291 PB - Springer Science + Business Medi CY - Heidelberg, London, New York ER - TY - GEN A1 - Kulikov, Valentin A1 - Mirsky, Vladimir M. A1 - Delaney, Tetyana L. A1 - Donoval, Daniel A1 - Koch, Alexander W. A1 - Wolfbeis, Otto S. T1 - High-throughput analysis of bulk and contact conductance of polymer layers prepared by combinatorial electropolymerization T2 - Measurement Science and Technology N2 - An approach for high-throughput analysis of bulk and contact conductance of polymer layers is described and evaluated. The approach, based on s24-technique (simultaneous two- and four-point conductance measurements), was realized as a high-throughput method and applied for investigation of conductive polymers on an array of interdigital platinum electrodes. Several examples demonstrate distinctive influence of combinatorially varied conditions of polymer synthesis (polymerization charge, content of copolymers) as well as chemical treatment of the synthesized polymers in bulk and contact resistance of metal/polymer/metal systems. The developed high-throughput s24-technique can be widely applied to material research for investigation of bulk and contact electrical properties. Y1 - 2005 U6 - https://doi.org/10.1088/0957-0233/16/1/013 SN - 1361-6501 SN - 0957-0233 VL - 16 IS - 1 SP - 95 EP - 99 ER - TY - GEN A1 - Ando, Masanori A1 - Swart, Claudia A1 - Pringsheim, Erika A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Optical ozone sensing properties of poly(2-chloroaniline), poly(N-methylaniline) and polyaniline films T2 - Sensors and Actuators B: Chemical N2 - The ozone-sensitive visible/near-IR absorbance changes of poly(2-chloroaniline) (P(2ClANI)), poly(N-methylaniline) (P(NMeANI)) and polyaniline (PANI) films were investigated. These three polymer films, which were assumed to be present partially in a protonated form and partially in a reduced form, were prepared by chemical oxidation of the monomers followed by treatment with the reducing agent disodium disulfite. The P(2ClANI) film was sensitive to ozone at concentrations of down to 2.5 ppm, but saturation of sensitivity started to appear above 50 ppm. By contrast, the PANI and P(NMeANI) films were sensitive to ozone in a concentration range of 50–100 ppm without showing saturation of sensitivity. KW - Optical ozone sensing KW - Poly(2-chloroaniline) film KW - Poly(N-methylaniline) film KW - Polyaniline film KW - Visible/near-IR absorbance change Y1 - 2005 U6 - https://doi.org/10.1016/j.snb.2004.12.083 SN - 0925-4005 VL - 108 IS - 1-2 SP - 528 EP - 534 ER - TY - GEN A1 - Ando, Masanori A1 - Swart, Claudia A1 - Pringsheim, Erika A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Ozone sensing properties of polyaniline derivatives T2 - Chemical Sensors (Suppl. B) Y1 - 2004 VL - 20 SP - 534 EP - 535 ER - TY - GEN A1 - Hao, Qingli A1 - Kulikov, Valentin A1 - Mirsky, Vladimir M. T1 - Investigation of contact and bulk resistance of conductive polymers by simultaneous two- and four-point technique T2 - Sensors and Actuators B: Chemical N2 - A conventional resistance measured by two-point technique includes bulk and contact components. The contact resistance can be excluded by a four-point technique. A simultaneous use of the two- and four-point techniques allows to separate the total resistance between two electrodes for the contact and bulk parts. This approach has been used to analyze electrical coupling between metal electrodes of interdigitated structures and chemically sensitive coating formed by polyaniline (PANI). The polymer layers were deposited by electropolymerization on bare gold and platinum electrodes as well as on gold electrodes precoated by a self-assembled monolayer of 4-aminothiophenol. An exposure of the PANI films formed on interdigitated structures to gaseous HCl displays proportional changes of resistance measured by the two- and four- point techniques for platinum electrodes and for the precoated gold electrode, while a strong deviation from this proportionality was observed for the gold electrodes without precoating. The results demonstrate a high contribution of the contact resistance between PANI and bare gold electrodes into the resistance measured by the two-point technique. This contribution is small and therefore not measurable for bare platinum electrodes or for gold electrodes coated by 4-aminothiophenol. KW - Chemoresistor KW - Conductometric chemosensor KW - Electropolymerization KW - Four-point technique KW - Gas sensor Y1 - 2003 U6 - https://doi.org/10.1016/S0925-4005(03)00456-8 SN - 0925-4005 VL - 94 IS - 3 SP - 352 EP - 357 ER - TY - GEN A1 - Hao, Qingli A1 - Rahm, Michael A1 - Weiss, Dieter A1 - Mirsky, Vladimir M. T1 - Morphology of electropolymerized poly (N-methylaniline) films T2 - Microchimica Acta N2 - The influence of polymerization conditionson the morphology of thin films of poly-(N-methylani-line) (PNMA) was studied. The films were prepared byelectropolymerization on gold and platinum electrodesand studied by optical and electron microscopy. It wasdemonstrated that the film morphology does notdepend on the polymerization rate or on the electrodemetal.However,itissensitivetothekindofcounterionofPNMA.Thepolymerscontainingacetate orchloridedo not form stable films. Polymer films containingphosphate or sulfate display inhomogeneous struc-tures. A formation of sprouts and ‘‘nanotubes’’ wasobserved for PNMA sulfate. The films formed byPNMA perchlorate are smooth and transparent(glass-likestructure).Asublayerof4-aminothiophenolincreases the adhesion of polymers to the gold surface. KW - Poly-(N-methylaniline) KW - polyaniline derivatives KW - electropolymerization KW - film morphology Y1 - 2003 U6 - https://doi.org/10.1007/s00604-003-0063-8 SN - 0026-3672 SN - 1436-5073 VL - 143 IS - 2 SP - 147 EP - 153 ER - TY - GEN A1 - Wrobel, Nadia A1 - Deininger, Werner A1 - Hegemann, Peter A1 - Mirsky, Vladimir M. T1 - Covalent immobilization of oligonucleotides on electrodes T2 - Colloids and Surfaces B: Biointerfaces N2 - 32P-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20% of the value calculated for a densely packed monolayer. KW - DNA KW - DNA immobilization KW - DNA sensor KW - Biosensor KW - Hybridization Y1 - 2003 U6 - https://doi.org/10.1016/S0927-7765(03)00155-3 SN - 0927-7765 VL - 32 IS - 2 SP - 157 EP - 162 ER - TY - GEN A1 - Prodramidis, Mamas I. A1 - Hirsch, Thomas A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Enantioselective chemosensors based on spreader-bar structures T2 - Electroanalysis N2 - Chiroselective binding sites have been created on thin gold films by application of the spreader-bar approach. Impedometric techniques and surface plasmon resonance were applied to detect binding. (R)-(+)-1,1 '-Binaphthyl-2,2 '-diol (R-BNOH) and (S)-(-)-1,1 '-binaphthyl-2,2'-diol (S-BNOH) were used as model analytes. The artificial receptors were prepared by co-adsorption of 16-mercaptohexadecane (matrix) with a thiol-modified chiral selector (template). The conjugates of d, l-thioctic acid and (R)-(+)- or (S)-(-)-1,1'-binaphthyl-2,2'-diamine were used as templates. Different concentration ratios of the matrix and template were tested. No chiral selectivity of surfaces formed by either the matrix or the template alone was observed. The use of alkylthiols shorter than 16-mercaptohexadecane led to the formation of surfaces with no chiral selectivity. The gold electrodes coated by the spreader-bar technique displayed an enantioselectivity of up to 4.76 or up to 2.55 as measured by the capacitive and SPR methods, espectively. KW - Enantioselective artificial receptors KW - Spreader-bar technique KW - Capacitive chemosensor KW - Surface plasmon resonance Y1 - 2003 U6 - https://doi.org/10.1002/elan.200302756 SN - 1521-4109 VL - 15 IS - 22 SP - 1795 EP - 1798 ER - TY - CHAP A1 - Mirsky, Vladimir M. A1 - Kulikov, Valentin ED - Potyrailo, Radislav A. ED - Amis, Eric J. T1 - Combinatorial electropolymerization: concept, equipment and applications T2 - High Throughput Analysis: A Tool for Combinatorial Materials Science Y1 - 2003 SN - 0-306-47758-0 SP - 431 EP - 446 PB - Kluwer Academic Publishers CY - New York ER - TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive sensors based on photografted molecularly imprinted polymers T2 - Biocybernetics and Biomedical Engineering N2 - The reversible chemosensors to creatinine and desmetryn based on artificial chemoreceptors are described. A grafted photopolymerization combined with a technique of molecular imprinting was used for the receptor layer preparation. After polymer deposition and intensive washing from templates, the electrodes were investigated by impedance spectrometry. Creatinine and desmetryn binding were detected as a decrease in the electrode capacitance. Sensor response to creatining was highly selective. No response to the addition of sodium chloride, creatine, urea or glucose were observed. The electrodes coated with the molecularly imprinted polymers selective to desmetryn displayed specific binding of this herbicide: only small capacitive effects were observed to addition of terbumeton or atrazine, while metribuzine displayed capacitance decrease similar to desmetryn. KW - capacitive sensor KW - creatinine KW - atrazine KW - chemical sensor KW - photografted polymerization Y1 - 2001 SN - 0208-5216 VL - 21 IS - 3 SP - 43 EP - 54 ER - TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Ulbricht, Mathias A1 - Wolfbeis, Otto S. T1 - Impedometric herbicide chemosensors based on molecularly imprinted polymers T2 - Analytica Chimica Acta N2 - The technique of grafting polymerization has been used for preparation of thin films of molecularly imprinting polymers on the surface of polypropylene membranes and on hydrophobized gold electrodes. The herbicide desmetryn was used as a template. The solid supports used were hydrophobic, while the polymer was hydrophilic. The adsorbed layer of benzophenone, irradiated by UV-light, initiated a radical polymerization near the surface. Polymer films were characterized by weighing, contact angle measurements and impedance spectroscopy. The electrodes coated with the molecularly imprinted polymers displayed fairly specific binding of desmetryn, as detected by the decrease in the capacitance of the electrode. Only small capacitive effects were observed on addition of terbumeton or atrazine, while metribuzine displayed capacitance decrease similar to desmetryn. KW - Molecular imprinting KW - Capacitive sensor KW - Chemical sensor KW - Grafting polymerization KW - Photopolymerization Y1 - 2001 U6 - https://doi.org/10.1016/S0003-2670(00)01280-0 SN - 0003-2670 VL - 435 IS - 1 SP - 157 EP - 162 ER - TY - GEN A1 - Vasjari, Majlinda A1 - Mirsky, Vladimir M. T1 - Calibrated nanoinjections of mercury vapor T2 - Fresenius' Journal of Analytical Chemistry N2 - A simple technique for the calibration of mercury vapor chemosensors is described. It is based on a reductive deposition of a well-defined quantity of mercury (0) onto gold wire followed by thermal evaporation of the mercury into the sensor cell. The quantity of mercury on the gold wire was measured electrochemically by oxidation before and after heating, as well as after storage under different conditions. It is shown that the technique can be used for calibrated injections of nanogram quantities of mercury. The approach was applied to test the performance of ultrasensitive mercury chemoresistors. Y1 - 2000 SN - 1618-2642 SN - 1618-2650 VL - 368 IS - 7 SP - 727 EP - 729 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Wrobel, Nadia A1 - Riepl, Michael A1 - Hirsch, Thomas A1 - Wolfbeis, Otto S. T1 - Intelligente Nanostrukturen für Biosensoren und Sensor-Arrays T2 - BioTec: Zeitschrift für Biotechnologie Y1 - 1999 SN - 0937-2725 VL - 10 SP - 28 EP - 32 ER - TY - CHAP A1 - Wrobel, Nadia A1 - Riepl, Michael A1 - Schinkinger, Manfred A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive thin film biosensors for bacteriophages and nucleic acids T2 - Proceedings, EMBEC ’99, November 4 - 7, 1999, Vienna, Austria Y1 - 1999 SP - 362 EP - 363 PB - IFMBE CY - Stevenage ER - TY - GEN A1 - Panasyuk-Delaney, Tanya A1 - Mirsky, Vladimir M. A1 - Piletsky, Sergey A. A1 - Wolfbeis, Otto S. T1 - Electropolymerized molecularly imprinted polymers as receptor layers in capacitive chemical sensors T2 - Analytical Chemistry N2 - The first application of molecularly imprinted polymers to chemical sensors with capacitive detection is described. The sensitive layer was prepared by electropolymerization of phenol on gold electrodes in the presence of the template (phenylalanine). The insulating properties of the polymer layer were studied by electrochemical impedance spectroscopy. Electrical leakages through the polymer layer were suppressed by deposition of a self-assembled monolayer of mercaptophenol before polymerization and of alkanethiol after polymerization. At the final stage of sensor preparation, the template was removed. The multilayer system obtained displayed a decrease in electrical capacitance on addition of phenylalanine. Only a low response was observed toward other amino acids and phenol. Y1 - 1999 U6 - https://doi.org/10.1021/ac9903196 SN - 0003-2700 SN - 1520-6882 VL - 71 IS - 20 SP - 4609 EP - 4613 ER - TY - GEN A1 - Hirsch, Thomas A1 - Kettenberger, Hubert A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - A simple strategy for preparation of sensor arrays: Nanostructured monolayers as recognition elements T2 - Chemical Communications N2 - The spreader-bar approach is a simple method for producing a huge variety of receptors with different selectivities. A sensor-array consisting of five such receptors is presented. A pattern recognition provides selective detection of different purines and pyrimidines. Y1 - 2003 U6 - https://doi.org/10.1039/b210554c SN - 1359-7345 SN - 1364-548X IS - 3 SP - 432 EP - 433 ER -