TY - CHAP A1 - Mirsky, Vladimir M. ED - Potyrailo, Radislav A. ED - Mirsky, Vladimir M. T1 - Combinatorial development of chemosensitive conductive polymers T2 - Combinatorial Methods for Chemical and Biological Sensors N2 - Conductive polymers are established materials for development of chemical and biological sensors. Properties of these polymers are influenced by a number of different physical and chemical factors. Application of combinatorial and high-throughput techniques to development and optimization of chemo and biosensors is reviewed. Methods for addressable synthesis of conductive polymers and protocols for comprehensive description of chemosensitive properties are discussed. Y1 - 2009 SN - 978-0-387-73712-6 U6 - https://doi.org/10.1007/978-0-387-73713-3_13 SP - 315 EP - 330 PB - Springer CY - New York, NY [u.a.] ER - TY - GEN A1 - Potyrailo, Radislav A. A1 - Mirsky, Vladimir M. T1 - Combinatorial and high-throughput development of sensing materials: The first ten years T2 - Chemical Reviews Y1 - 2008 U6 - https://doi.org/10.1021/cr068127f SN - 0009-2665 SN - 1520-6890 VL - 108 SP - 770 EP - 813 ER - TY - GEN A1 - Zybin, Alexander A1 - Boecker, Daniel A1 - Mirsky, Vladimir M. A1 - Niemax, Kay T1 - Enhancement of the detection power of surface plasmon resonance measurements by optimization of the reflection angle T2 - Analytical Chemistry N2 - The detection limit of surface plasmon resonance (SPR) measurements has been improved by a factor of approximately 2-3.5 if the angle of incidence was near to the reflection minimum of the SPR resonance curve instead at the position of the steepest slope, the standard alignment in SPR imaging. The enhancement of the detection power, a result of signal-to-noise optimization, is demonstrated by applying a photodiode and a CCD camera for SPR detection. The experimental data are compared with data expected from theory. Y1 - 2007 U6 - https://doi.org/10.1021/ac070074u SN - 0003-2700 SN - 1520-6882 VL - 79 IS - 11 SP - 4233 EP - 4236 ER - TY - GEN A1 - Delaney, Tetyana L. A1 - Zimin, Denys A1 - Rahm, Michael A1 - Weiss, Dieter A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Capacitive detection in ultrathin chemosensors prepared by molecularly imprinted grafting photopolymerization T2 - Analytical Chemistry N2 - The usual applications of capacitive detection in chemo- and biosensors are based on changes in effective thickness of insulating layers due to adsorption of analyte onto receptors. Ultrathin chemosensors based on molecularly imprinted polymerization enable a realization of another capacitive approach that exploits changes in electrical capacitance due to modification of the dielectric constant of the polymer. Such chemosensors were prepared by photografted molecularly imprinted polymerization on the surface of gold electrodes. An adsorbed layer of hydrophobic photoinitiator (benzophenone) provided grafted polymerization on the surface of the alkanethiol-modified gold electrode. The chemosensors were characterized by cyclic voltammetry, impedance spectroscopy, and scanning electron and atomic force microscopy. Binding of analyte was detected by measurements of electrical capacitance. The results indicate a decrease of the dielectric constant of the polymer layer due to analyte binding up to 20%. Y1 - 2007 U6 - https://doi.org/10.1021/ac062143v SN - 0003-2700 SN - 1520-6882 VL - 79 IS - 8 SP - 3220 EP - 3225 ER - TY - CHAP A1 - Mirsky, Vladimir M. ED - Corain, Benedetto ED - Schmid, Günter ED - Toshima, Naoki T1 - Spreader-bar structures as molecular templates for electrochemical synthesis of nanoparticles T2 - Metal nanoclusters in catalysis and materials science: the issue of size-control Y1 - 2008 U6 - https://doi.org/10.1016/B978-044453057-8.50020-9 SP - 321 EP - 325 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Hirsch, Thomas A1 - Shaporenko, Andrey A1 - Mirsky, Vladimir M. A1 - Zharnikov, Michael T1 - Monomolecular films of phthalocyanines: formation, characterisation and expelling by alkanethiols T2 - Langmuir N2 - Adsorption of aluminum-2,3-naphthalocyanine (Al-PC) onto gold (111) substrate from the pure and mixed (with alkanethiols) solutions of the target molecules in ethanol was studied. The resulting films were characterized by X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure spectroscopy. The adsorption from the pure solution resulted in formation of an Al-PC monolayer composed of the strongly inclined molecules. However, a presence of only one molar percent of a thiolated compound (alkanethiol) in the primary solution led to the complete expelling of the Al-PC molecules from the substrate and formation of a one-component alkylthiolate monolayer. The results indicate that an equilibrium formation of mixed monolayers of rodlike thiolated molecules and relatively large planar-geometry molecules, whose interaction with the metal surface is mainly provided by the π-electron system, is difficult to achieve or is in most cases even impossible under equilibrium conditions and requires an introduction of additional anchor moieties (e.g., thiols) into these molecules, as has been demonstrated by successful coadsorption of hexadecanethiol and thiolated tetraphenylporphyrin. Y1 - 2007 U6 - https://doi.org/10.1021/la0634239 SN - 0743-7463 SN - 1520-5827 VL - 23 IS - 8 SP - 4373 EP - 4377 ER - TY - GEN A1 - Cao, Limin A1 - Lin, Hong A1 - Mirsky, Vladimir M. T1 - DNA-based surface plasmon resonance biosensor for enrofloxacin T2 - Analytica Chimica Acta N2 - A DNA-based surface plasmon resonance biosensor for enrofloxacin was developed. Heating denatured DNA immobilized on the gold-coated glass surface was exploited. The immobilization was performed by a layer-by-layer co-deposition with a cationic polymer. The sensor performance was tested with real biological probes. Direct and simple determination of enrofloxacin in milk samples was demonstrated. The sensor response obeys Langmuir binding isotherm being almost linear until about 20 µg per mL. The detection limit in milk samples was estimated to be 3 µg per mL. KW - Surface plasmon resonance KW - Nucleic acid KW - Biosensor KW - Enrofloxacin KW - Fluoroquinolones KW - Layer-by-layer deposition Y1 - 2007 U6 - https://doi.org/10.1016/j.aca.2007.02.034 SN - 0003-2670 VL - 589 IS - 1 SP - 1 EP - 5 ER - TY - GEN A1 - Cao, Limin A1 - Lin, Hong A1 - Mirsky, Vladimir M. T1 - Detection of antibiotics in food: Extraction of fluoroquinolones by DNA T2 - Analytical and Bioanalytical Chemistry N2 - The ability of DNA to extract fluoroquinolones from model solutions and real probes of food was demonstrated and investigated quantitatively. The interaction between fluoroquinolones and different types of DNA was studied by equilibrium dialysis. The first application of this direct approach allowed us to determine binding constants and binding stoichiometries in different conditions. The binding of enrofloxacin to heat-denatured DNA (d-DNA) from herring sperm is pH- and magnesium-dependent; the highest fraction of bound drugs was found at pH 7 and a magnesium concentration of 0.5–1 mM. Results for three types of DNA: d-DNA, double-stranded DNA and single-stranded DNA were compared. The unwound DNA showed almost doubled binding constants and stoichiometries, thus indicating preferable interaction of enrofloxacin with single-strand regions of DNA. The binding of other fluoroquinolones (lomefloxacin, ciprofloxacin, norfloxacin, danofloxacin and sarafloxacin) with d-DNA is very similar to that of enrofloxacin: the binding constants are in the range from 0.94 × 105 to 2.40 × 105 M−1, and the stoichiometries range from 4.1 to 6.9 fluoroquinolone molecules per 100 DNA bases. The binding properties were quantitatively the same for extraction of fluoroquinolones from model aqueous solutions and from liquid food (milk). The results indicate the efficiency of DNA for selective extraction of fluoroquinolones from real samples for further analysis. This selective binding also allows us to consider DNA as a natural receptor for development of analytical techniques for fluoroquinolones. KW - Fluoroquinolone KW - DNAExtraction KW - Binding constant KW - Binding stoichiometry Y1 - 2007 U6 - https://doi.org/10.1007/s00216-007-1191-5 SN - 1618-2642 SN - 1618-2650 VL - 388 IS - 1 SP - 253 EP - 258 ER - TY - CHAP A1 - Vasjari, Majlinda A1 - Mirsky, Vladimir M. ED - Alegret, Salvador ED - Merkoci, Arben T1 - Chemoresistor for determination of mercury vapor T2 - Electrochemical Sensor Analysis N2 - This chapter presents a procedure to prepare and calibrate chemical sensor for mercury vapor and discusses application of the sensor for measurements of mercury vapor. The sensor is based on high affinity of gold to mercury and on chemoresistive properties of ultrathin gold layers: adsorption of mercury leads to increase in the surface resistance. However, this effect is not selective: similar changes are caused by adsorption of water vapor and sulfuric compounds. The use of monomolecular layer of alkylthiols as a filter excludes this interference completely. Sensor calibration is performed by thermoinjection of nanogram-amount of mercury quantitatively deposited by electrochemical reduction. It is found that the sensors display no sensitivity to such typical interferents of mercury chemoresistors based on uncoated gold as volatile thiols and water. Preliminary data demonstrate a possibility to measure Hg(0) even in aqueous solutions. Y1 - 2007 SN - 978-0-444-53053-0 U6 - https://doi.org/10.1016/S0166-526X(06)49058-9 SP - 105 EP - 109 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Mirsky, Vladimir M. A1 - Vasjari, Majlinda ED - Alegret, Salvador ED - Merkoci, Arben T1 - Chemical sensors for mercury vapour T2 - Electrochemical Sensor Analysis N2 - A number of analytical methods were developed for determination of elemental mercury. They include traditional analytical techniques, such as atomic adsorption spectroscopy (AAS), atomic fluorescence spectroscopy (AFS), and atomic emission spectroscopy (AES). Other analytical methods include neutron-activation analysis and laser photo-acoustic spectroscopy (detection limit about 5 μg/l). To increase sensitivity and selectivity of these methods, a preconcentration of mercury is widely performed. This can be realized by exploring high affinity of mercury to gold. Mercury and its volatile compounds can easily be adsorbed on gold at normal temperatures and can be released quantitatively upon heating. Hence, gold has been used as an adsorbent for the accumulation of mercury to a concentration sufficient for further spectroscopic analyses. The main disadvantage of mercury sensors based on bare gold layers is their poor selectivity and applications of such sensors for real probes and makes necessary a pre-treatment of probes. Mercury sensors based on thin gold layers require regular calibrations. In ideal cases, the calibration should be performed before each measurement. Therefore, a calibration technique should be compatible with design and concept of the mercury vapor sensor. Y1 - 2007 SN - 978-0-444-53053-0 U6 - https://doi.org/10.1016/S0166-526X(06)49012-7 SP - 235 EP - 251 PB - Elsevier CY - Amsterdam ER -