TY - GEN A1 - Whale, Alexandra S. A1 - Spiegelaere, Ward De A1 - Trypsteen, W. A1 - Nour, Afif Abdel A1 - Bae, Young-Kyung A1 - Benes, Vladimir A1 - Cleveland, Megan A1 - Devonshire, Alison S. A1 - Dong, Lianhua A1 - Drandi, Daniela A1 - Foy, Carole A. A1 - Garson, Jeremy A. A1 - He, Hua-Jun A1 - Hellemans, Jan A1 - Kubista, Mikael A1 - Lievens, Antoon A1 - Makrigiogos, Mike G. A1 - Müller, Reinhold D. A1 - Nolan, Tania A1 - O'Sullivan, Denise M. A1 - Pfaffl, Michael W. A1 - Rödiger, Stefan A1 - Romsos, Erica L. A1 - Shipley, Gregory L. A1 - Taly, Valerie A1 - Untergasser, Andreas A1 - Wittwer, Carl T. A1 - Bustin, Stephen A. A1 - Vandesompele, Jo A1 - Huggett, Jim F. T1 - The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 T2 - Clinical Chemistry Y1 - 2020 U6 - https://doi.org/10.1093/clinchem/hvaa125 SN - 0009-9147 SN - 1530-8561 VL - 66 IS - 8 SP - 1012 EP - 1029 ER - TY - GEN A1 - Bustin, Stephen A A1 - Ruijter, Jan M A1 - van den Hoff, Maurice J B A1 - Kubista, Mikael A1 - Pfaffl, Michael W A1 - Shipley, Gregory L A1 - Tran, Nham A1 - Rödiger, Stefan A1 - Untergasser, Andreas A1 - Mueller, Reinhold A1 - Nolan, Tania A1 - Milavec, Mojca A1 - Burns, Malcolm J A1 - Huggett, Jim F A1 - Vandesompele, Jo A1 - Wittwer, Carl T T1 - MIQE 2.0 : revision of the minimum information for publication of quantitative real-time PCR experiments guidelines T2 - Clinical chemistry N2 - Background In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for the design, execution, and reporting of quantitative PCR (qPCR) in research. The expansion of qPCR into numerous new domains has driven the development of new reagents, methods, consumables, and instruments, requiring revisions to best practices that are tailored to the evolving complexities of contemporary qPCR applications. Content Transparent, clear, and comprehensive description and reporting of all experimental details are necessary to ensure the repeatability and reproducibility of qPCR results. These revised MIQE guidelines reflect recent advances in qPCR technology, offering clear recommendations for sample handling, assay design, and validation, along with guidance on qPCR data analysis. Instrument manufacturers are encouraged to enable the export of raw data to facilitate thorough analyses and re-evaluation by manuscript reviewers and interested researchers. The guidelines emphasize that quantification cycle (Cq) values should be converted into efficiency-corrected target quantities and reported with prediction intervals, along with detection limits and dynamic ranges for each target, based on the chosen quantification method. Additionally, best practices for normalization and quality control are outlined and reporting requirements have been clarified and streamlined. The aim is to encourage researchers to provide all necessary information without undue burden, thereby promoting more rigorous and reproducible qPCR research. Summary Building on the collaborative efforts of an international team of researchers, we present updates, simplifications, and new recommendations to the original MIQE guidelines, designed to maintain their relevance and applicability in the context of emerging technologies and evolving qPCR applications. Y1 - 2025 U6 - https://doi.org/10.1093/clinchem/hvaf043 SN - 0009-9147 VL - 71 IS - 6 SP - 634 EP - 651 PB - Oxford University Press (OUP) CY - Washington, DC ; Oxford ER -