TY - GEN A1 - Ingenbosch, Kim N. A1 - Quint, Stephan A1 - Dyllick-Brenzinger, Melanie A1 - Wunschik, Dennis S. A1 - Kiebist, Jan A1 - Süss, Philipp A1 - Liebelt, Ute A1 - Zuhse, Ralf A1 - Menyes, Ulf A1 - Scheibner, Katrin A1 - Mayer, Christian A1 - Opwis, Klaus A1 - Gutmann, Jochen S. A1 - Hoffmann-Jacobsen, Kerstin T1 - Singlet oxygen generation by peroxidases and peroxygenases for chemo-enzymatic synthesis T2 - ChemBioChem N2 - Singlet oxygen is a reactive oxygen species undesired in living cells but a rare and valuable reagent in chemical synthesis. We present a fluorescence spectroscopic analysis of the singlet‐oxygen formation activity of commercial peroxidases and novel peroxygenases. Singlet‐oxygen sensor green (SOSG) is used as fluorogenic singlet oxygen trap. Establishing a kinetic model for the reaction cascade to the fluorescent SOSG endoperoxide permits a kinetic analysis of enzymatic singlet‐oxygen formation. All peroxidases and peroxygenases show singlet‐oxygen formation. No singlet oxygen activity could be found for any catalase under investigation. Substrate inhibition is observed for all reactive enzymes. The commercial dye‐decolorizing peroxidase industrially used for dairy bleaching shows the highest singlet‐oxygen activity and the lowest inhibition. This enzyme was immobilized on a textile carrier and successfully applied for a chemical synthesis. Here, ascaridole was synthesized via enzymatically produced singlet oxygen. KW - Peroxygenase Y1 - 2021 U6 - https://doi.org/10.1002/cbic.202000326 SN - 1439-7633 SN - 1439-4227 VL - 22 IS - 2 SP - 398 EP - 407 ER - TY - BOOK A1 - Anders, Kenneth A1 - Donat, Ralf A1 - Durka, Walter A1 - Elmer, Michael A1 - Fischer, Lars A1 - Gailing, Ludger A1 - Hoffmann, Melanie A1 - Hornemann, Gitte A1 - Hüttl, Reinhard F. A1 - Peschel, Tim A1 - Rißmann, Cornelia A1 - Röhring, Andreas A1 - Siedschlag, Yvonne A1 - Pilarski, Monika A1 - Vetter, Andreas A1 - Vorwald, Jörn A1 - Wöllecke, Jens A1 - Wiegleb, Gerhard A1 - Xylander, Willi E. R. ED - Anders, Kenneth ED - Fischer, Lars ED - Röhring, Andreas ED - Peschel, Tim ED - Donat, Ralf T1 - Neulandschafft - Leben in den Schlabendorfer Feldern - Natur und Mensch : eine Publikation der Landschaftswerkstatt Schlabendorfer Felder im Rahmen des Forschungsverbundes SUBICON Y1 - 2010 PB - Büro für Landschaftskommunikation CY - Bad Freienwalde ER - TY - BOOK A1 - Hoffmann, Melanie A1 - Anders, Kenneth A1 - Fischer, Lars A1 - Böllmann, Jörg A1 - Brückner, Ardo A1 - Brunk, Ingo A1 - Donath, Helmut A1 - Donat, Ralf A1 - Elmer, Michael A1 - Güth, Mareike A1 - Häußler, Robert A1 - Mrzljak, Jadranka A1 - Mudra, Dieter A1 - Müller, Markus A1 - Peschel, Tim A1 - Pilz, Wilfried A1 - Sawade, Wolfgang A1 - Schonert, P. A1 - Siedschlag, Marina A1 - Siedschlag, Yvonne A1 - Wanner, Manfred A1 - Wöllecke, Jens ED - Hoffmann, Melanie ED - Anders, Kenneth ED - Fischer, Lars T1 - Kolonisten gesucht! Steckbriefe natürlicher und sozialer Besiedler der Bergbaufolgelandschaft Schlabendorfer Felder Y1 - 2010 PB - Büro für Landschaftskommunikation CY - Bad Freienwalde ER -