TY - GEN A1 - Rehacek, Vlastimil A1 - Novotny, Ivan A1 - Tvarozek, Vladimir A1 - Riepl, Michael A1 - Hirsch, T. A1 - Mass, Markus A1 - Schweiss, R. A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. ED - Breza, Jura ED - Donoval, Daniel T1 - Capacitive detection in thin film chemical sensors and biosensors T2 - Advanced Semiconductor Devices and Microsystems N2 - Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented. Y1 - 1998 SN - 0-7803-4909-1 SP - 255 EP - 258 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Mass, Markus A1 - Hirsch, T. A1 - Schweiss, R. A1 - Wolfbeis, Otto S. ED - Vincenzini, Pietro ED - Dori, L. T1 - Capacitive detection of analyte binding in thin film chemo- and biosensors T2 - Solid State Chemical and Biochemical Sensors Y1 - 1999 SN - 88-86538-27-8 SP - 441 EP - 448 PB - Techna CY - Faenza ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Mass, Markus A1 - Krause, Christian A1 - Wolfbeis, Otto S. T1 - Capacitive approach to determine phospholipase A2 activity towards artificial and natural substrates T2 - Analytical Chemistry N2 - A capacitive approach has been employed to develop a novel method to determine phospholipase activity. The sensing electrodes have a structure like Au/S(CH₂)₁₇CH₃/substrate/electrolyte. Hydrolysis of the substrate, mediated by phospholipase A₂, leads to the formation of water-soluble products from the insoluble substrate. This results in desorption of these products into aqueous phase and corresponding increase of the electrode capacitance. The requirement of high water solubility of the reaction products can be achieved in two ways. In the first, short-chain phospholipids are used as the substrate, in which case, water-soluble products are formed and no additional reagents are required to promote desorption of these products. The sensors prepared by this strategy provide sensitive qualitative detection of phospholipases. The second way is based on the use of a water-soluble acceptor (for example, β-cyclodextrin) to solubilize the products of hydrolysis. It allows semiquantitative detection of phospholipase activity toward long-chain natural substrates. The reaction kinetics for this case was found to be monoexponential and linearly dependent on the phospholipase concentration. The detection limit of this method, as tested with phospholipase A₂ from bee venom and soy bean lecithin as the substrate, is ∼0.5 ng/mL (500 μunits/mL). Y1 - 1998 U6 - https://doi.org/10.1021/ac980102w SN - 0003-2700 SN - 1520-6882 VL - 70 IS - 17 SP - 3674 EP - 3678 ER -