TY - GEN A1 - Gerling, Charlotte A1 - Drechsler, Martin A1 - Keuler, Klaus A1 - Leins, Johannes A. A1 - Radtke, Kai A1 - Schulz, Björn A1 - Sturm, Astrid A1 - Wätzold, Frank T1 - Climate-ecological-economic modelling for the cost-effective spatiotemporal allocation of conservation measures in cultural landscapes facing climate change T2 - Q Open Y1 - 2022 U6 - https://doi.org/10.1093/qopen/qoac004 SN - 2633-9048 VL - 2 IS - 1 SP - 1 EP - 20 ER - TY - GEN A1 - Behren, Sascha von A1 - Turek, Maximilian A1 - Barthelmes, Lukas A1 - Scholta, Hanna A1 - Hansen, Frank A1 - Kagerbauer, Martin A1 - Eisenmann, Christine T1 - Decoding Urban Archetypes: Exploring Mobility-Related Homogeneity among Cities T2 - Sustainability N2 - To make cities more sustainable and livable and to achieve climate targets in transportation, cities around the globe must undergo sustainable transformations. However, disparities in initial conditions pose challenges when trying to implement these sustainable changes. Identifying these differences aids in the comprehension of future developments. In this study, we establish an international comparison by decoding the mobility-related characteristics of cities and determining urban archetypes. Using publicly accessible data, we analyze and classify 96 cities in different countries. Therefore, we utilize principal component analysis to simplify the data. The emerging components serve as input for segmentation. This approach yields nine unique urban archetypes, ranging from Well-Functioning and Ancient Hybrid Cities in Europe to Paratransit and Traffic-Saturated Cities in the southern hemisphere. Our results show that there is a significant advantage to using a multidimensional segmentation basis, which we identify in an extensive literature review. The result is a finer segmentation, which is especially clear for European cities that demonstrate four different clusters. We discuss that the effect of future restrictions on private car usage will vary widely between the urban archetypes. KW - urban mobility urban archetypes KW - car dependence KW - smart city KW - city typology KW - city comparison KW - sustainable mobility KW - multimodality KW - public transit KW - urban archetypes Y1 - 2023 U6 - https://doi.org/10.3390/su151914231 SN - 2071-1050 VL - 15 IS - 19 ER - TY - RPRT A1 - Behren, Sascha von A1 - Turek, Maximilian A1 - Barthelmes, Lukas A1 - Scholta, Hanna A1 - Hansen, Frank A1 - Kagerbauer, Martin A1 - Eisenmann, Christine T1 - Urban Archetypes N2 - To make cities more sustainable and livable and to achieve climate targets in transportation, cities around the globe must undergo sustainable transformations. However, disparities in initial conditions pose challenges when trying to implement these sustainable changes. Identifying these differences aids in the comprehension of future developments. Urban archetypes represent a typification of cities in order to investigate mobility-related homogeneity between cities. Y1 - 2023 UR - https://encyclopedia.pub/entry/52072 ER - TY - GEN A1 - Gerling, Charlotte A1 - Sturm, Astrid A1 - Wätzold, Frank A1 - Keuler, Klaus A1 - Radtke, Kai A1 - Drechsler, Martin A1 - Leins, Johannes A. T1 - Ökonomie der Klimaanpassung zum Schutz der biologischen Vielfalt ; Postervorstellung : BfN Statuskonferenz "Biodiversität und Klimawandel - Synergien erforschen" in Berlin, 7.12.2023 Y1 - 2023 U6 - https://doi.org/10.13140/RG.2.2.16540.97923 ER - TY - GEN A1 - Gerling, Charlotte A1 - Drechsler, Martin A1 - Leins, Johannes A. A1 - Sturm, Astrid A1 - Wätzold, Frank T1 - Cost-Effective Policy Instruments for Biodiversity Conservation Under Climate Change - the Need for Flexibility T2 - SSRN eLibrary Y1 - 2023 U6 - https://doi.org/10.2139/ssrn.4617809 SN - 1556-5068 SP - 1 EP - 60 ER - TY - GEN A1 - Gerling, Charlotte A1 - Drechsler, Martin A1 - Keuler, Klaus A1 - Sturm, Astrid A1 - Wätzold, Frank T1 - Time to consider the timing of conservation measures: Designing cost-effective agri-environment schemes under climate change T2 - Agricultural and Resource Economics Review Y1 - 2023 U6 - https://doi.org/10.1017/age.2023.4 SN - 2372-2614 SN - 1068-2805 VL - 52 IS - 2 SP - 231 EP - 249 ER - TY - GEN A1 - Gerling, Charlotte A1 - Drechsler, Martin A1 - Keuler, Klaus A1 - Leins, Johannes A1 - Schulz, Björn A1 - Sturm, Astrid A1 - Wätzold, Frank T1 - Effektivität und Kosteneffizienz von Artenschutzmaßnahmen unter Klimawandel - das Beispiel der Sumpfschrecke (Stethophyma grossum) in Schleswig-Holstein T2 - Natur und Landschaft Y1 - 2025 SN - 0028-0615-202501 VL - 100. Jahrgang 2025 IS - 1 SP - 2 EP - 8 ER - TY - GEN A1 - Martin, Frank A1 - Neubert, Annemarie A1 - Lutter, Anne-Helen A1 - Scholka, Jenny A1 - Hentschel, Erik A1 - Richter, Heiko A1 - Anderer, Ursula T1 - MTS, WST-8, and ATP viability assays in 2D and 3D cultures: Comparison of methodologically different assays in primary human chondrocytes T2 - Clinical Hemorheology and Microcirculation N2 - BACKGROUND: Tissue engineering enables the production of three-dimensional microtissues which mimic naturally occurring conditions in special tissues. These 3D culture systems are particularly suitable for application in regenerative medicine or experimental pharmacology and toxicology. Therefore, it is important to analyse the cells in their 3D microenvironment with regard to viability and differentiation. Tetrazolium assays (WST-8 and MTS) are still the methods of choice for estimating the number of living, metabolically active cells, with WST-8 being cell-impermeable compared to MTS. In contrast to these methods, the ATP assay is an endpoint method based on the luciferase-induced reaction of ATP with luciferin after cell lysis. OBJECTIVE:We compared three methodologically different proliferation/toxicity assays (MTS, WST-8, ATP) in monolayer (2D) and 3D culture systems to improve the technically challenging determination of the number of viable cells. METHODS: Chondrocytes were isolated from human articular cartilage. Three different test systems (MTS, WST-8, ATP) were applied to monolayer cells (2D, varying cell numbers) and spheroids (3D, different sizes) in 96-well plates. The intracellular ATP concentration was determined by luciferase-induced reaction of ATP with luciferin using a luminometer. Formazan formation was measured spectrophotometrically after different incubation periods. Evaluation was performed by phase contrast microscopy (toxicity), correlation of cell count and ATP concentration or absorption signal (Gompertz function) and propidium iodide (PI) staining to proof the cell lysis of all cells in spheroids. RESULTS: In 2D culture, all three assays showed a good correlation between the number of seeded cells and the ATP concentration or absorption data, whereas the MTS-assay showed the lowest specificity. In 3D culture, the spheroid sizes were directly related to the number of cells seeded. The absorption data of the WST-8 and MTS assay correlated only for certain spheroid size ranges, whereas the MTS-assay showed again the lowest specificity. Only the measured intracellular ATP content showed a linear correlation with all spheroid sizes ranging from 100–1000 µm. The WST-8 assay revealed the second-best sensitivity which allows the measurement of spheroids larger than 240 µm. Phase contrast observation of monolayer cells showed toxic effects of MTS after 6 h incubation and no signs of toxicity of WST-8. Staining with propidium iodide showed complete lysis of all cells in a spheroid in the ATP assay. CONCLUSION: Among tetrazolium-based assays, WST-8 is preferable to MTS because of its non-toxicity and better sensitivity. When determining the number of viable cells in the 2D system, caution is advised when using the ATP assay because of its two-phase slope of the correlation graph concerning cell number and intracellular ATP. In 3D systems of human chondrocytes, the ATP-assay is superior to the other two test systems, as the correlation graph between cell number and intracellular ATP is biphasic. Since differentiation processes or other metabolic events can influence the results of proliferation and toxicity assays (determination of viable cells), this should be taken into account when using these test systems. KW - ATP assay KW - MTS KW - WST-8 KW - Human chondrocytes KW - Spheroid KW - 3D culture KW - 2D culture Y1 - 2024 UR - https://journals.sagepub.com/doi/abs/10.3233/CH-248101 U6 - https://doi.org/10.3233/CH-248101 SN - ISSN 1386-0291 VL - 88 SP - 3 EP - 19 PB - IOS Press ER - TY - GEN A1 - Gomez de Santos, Patricia A1 - González-Benjumea, Alejandro A1 - Fernandez-Garcia, Angela A1 - Aranda, Carmen A1 - Wu, Yinqi A1 - But, Andrada A1 - Molina-Espeja, Patricia A1 - Maté, Diana M. A1 - Gonzalez-Perez, David A1 - Zhang, Wuyuan A1 - Kiebist, Jan A1 - Scheibner, Katrin A1 - Hofrichter, Martin A1 - Swiderek, Katarzyna A1 - Moliner, Vicent A1 - Sanz-Aparicio, Julia A1 - Hollmann, Frank A1 - Gutiérrez, Ana A1 - Alcalde, Miguel T1 - Engineering a highly regioselective fungal peroxygenase for the synthesis of hydroxy fatty acids T2 - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker N2 - The hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. In this study, we have engineered a highly regioselective fungal peroxygenase for the ω-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near the Phe catalytic tripod narrowed the heme cavity, promoting a dramatic shift toward subterminal hydroxylation with a drop in the over-oxidation activity. While crystallographic soaking experiments and molecular dynamic simulations shed light on this unique oxidation pattern, the selective biocatalyst was produced by Pichia pastoris at 0.4 g L−1 in a fed-batch bioreactor and used in the preparative synthesis of 1.4 g of (ω-1) hydroxytetradecanoic acid with 95 % regioselectivity and 83 % ee for the S enantiomer. KW - Peroxygenase Y1 - 2023 U6 - https://doi.org/10.1002/anie.202217372 SN - 1521-3773 VL - 62 IS - 9 SP - 1 EP - 9 PB - Wiley-VCH CY - Weinheim ER - TY - GEN A1 - Jiang, Yuyao A1 - Knaack, Marcus A1 - Martin, Clas Aljoscha A1 - Tost, Frank A1 - Alimov, Artem A1 - Härtel, Sebastian A1 - Gardill, Markus T1 - A 120 GHz industrial radar sensor network for condition monitoring of a forging process T2 - IEEE sensors journal N2 - Current trends in forging technology emphasize increased automation and a rising demand for high-precision components. Among the key factors influencing the forging quality are process parameters, which also serve as indispensable inputs for data-driven modeling. This necessitates the establishment of a stable and robust sensor network to enable high-quality process monitoring. This study investigates the implementation of a heterogeneous sensor network within an energy-bound forging press, integrating industrial 120 GHz radar sensors for comprehensive data collection. A complete radar signal processing chain is developed, encompassing signal acquisition, pre-processing, and state estimation of the press ram. The validation against conventional reference sensors confirms the reliability and accuracy of the radar-based measurements. The results demonstrate the feasibility of the radar employment in the complex forging environment. KW - Sensor network KW - FMCW radar KW - Kalman filter KW - Process parameter KW - Condition monitoring KW - Hot forging Y1 - 2025 U6 - https://doi.org/10.1109/JSEN.2025.3636060 SN - 1558-1748 SP - 1 EP - 10 PB - IEEE CY - Piscataway, NJ ER - TY - GEN A1 - Gerling, Charlotte A1 - Drechsler, Martin A1 - Leins, Johannes A. A1 - Sturm, Astrid A1 - Wätzold, Frank T1 - Cost-effective instruments for biodiversity conservation under climate change - the need for flexibility T2 - Ecological economics N2 - Climate change is one of the main threats for biodiversity. As it affects the ecological and economic system, conservation costs and impacts may change in a heterogeneous manner. This implies that cost-effective conservation sites and measures may no longer be so in the future. We investigate spatial flexibility (to adapt the location of conservation sites) and management flexibility (to adapt the conservation measures on those sites) as novel criteria for analysing the cost-effectiveness of policy instruments to conserve biodiversity under climate change. We develop a generic climate-ecological-economic modelling approach that captures the role of spatial and management flexibility to assess the cost-effectiveness of policy instruments. We apply the modelling approach to the conservation of an indicator species in agricultural grasslands in a case study area in Northern Germany, and compare the cost-effectiveness of the policy instruments of land purchase (low spatial flexibility, high management flexibility) and long-term individual conservation contracts (medium spatial and management flexibility) considering a period from 2020 to 2079. We find that both spatial and management flexibility matter in the case study and discuss their broader relevance for conservation in a changing climate. KW - Adaptation KW - Agricultural landscape KW - Ecological-economic modelling KW - Policy instrument evaluation KW - Tool Y1 - 2025 U6 - https://doi.org/10.1016/j.ecolecon.2024.108414 SN - 0921-8009 VL - 227 SP - 1 EP - 13 PB - Elsevier BV CY - Amsterdam ER - TY - GEN A1 - Gerling, Charlotte A1 - Drechsler, Martin A1 - Kadir, Karmand A1 - Kahlau, Bojan A1 - Leins, Johannes A1 - Sturm, Astrid A1 - Wätzold, Frank T1 - ClimeHop : an interactive app for teaching cost-effective biodiversity conservation under climate change T2 - The journal of economic education Y1 - 2025 U6 - https://doi.org/10.1080/00220485.2025.2461066 SN - 2152-4068 VL - 56 IS - 2 SP - 202 EP - 203 PB - Routledge, Taylor & Francis CY - Abingdon, Oxon ER - TY - GEN A1 - Fischer, Rico A1 - Drechsler, Martin A1 - Frank, Karin A1 - Berger, Uta A1 - Wang, Hsiao-Hsuan A1 - Semeniuk, Christina A1 - Armstrong, Amanda A1 - Grimm, Volker T1 - Ecological modelling for transormation T2 - Ecological modelling Y1 - 2025 U6 - https://doi.org/10.1016/j.ecolmodel.2025.111119 VL - 507 SP - 1 EP - 5 PB - Elsevier BV CY - Amsterdam ER -