TY - GEN A1 - Bimböse, Martin A1 - Nicolay, Alexander A1 - Bryk, Alexander A1 - Schmidt, Karl-Heinz A1 - Morche, David T1 - Investigations on intra- and interannual coarse sediment dynamics in a high-mountain catchment T2 - Zeitschrift für Geomorphologie : Supplementband Y1 - 2011 UR - http://www.schweizerbart.de/papers/zfg_suppl/detail/55/75985/Investigations_on_intra_and_interannual_coarse_sediment_dynamics_in_a_high_mountain_river U6 - https://doi.org/10.1127/0372-8854/2011/0055S2-0046 VL - 55 IS - 2 SP - 67 EP - 81 ER - TY - GEN A1 - Tolksdorf, Johann Friedrich A1 - Kaiser, Knut A1 - Petr, Libor A1 - Herbig, Christoph A1 - Kočár, Petr A1 - Heinrich, Susann A1 - Wilke, Franziska D.H. A1 - Theuerkauf, Martin A1 - Fülling, Alexander A1 - Schubert, Matthias A1 - Schröder, Frank A1 - Křivánek, Roman A1 - Schulz, Lars A1 - Bonhage, Alexander A1 - Hemker, Christiane T1 - Past human impact in a mountain forest: geoarchaeology of a medieval glass production and charcoal hearth site in the Erzgebirge, Germany T2 - Regional Environmental Change Y1 - 2020 U6 - https://doi.org/10.1007/s10113-020-01638-1 SN - 1436-378X SN - 1436-3798 VL - 20 IS - 3 ER - TY - GEN A1 - Pfetsch, Marc E. A1 - Fügenschuh, Armin A1 - Geißler, Björn A1 - Geißler, Nina A1 - Gollmer, Ralf A1 - Hiller, Benjamin A1 - Humpola, Jesco A1 - Koch, Thorsten A1 - Lehmann, Thomas A1 - Martin, Alexander A1 - Morsi, Antonio A1 - Rövekamp, Jessica A1 - Schewe, Lars A1 - Schmidt, Martin A1 - Schultz, Rüdiger A1 - Schwarz, Robert A1 - Schweiger, Jonas A1 - Stangl, Claudia A1 - Steinbach, Marc C. A1 - Vigerske, Stefan A1 - Willert, Bernhard M. T1 - Validation of Nominations in Gas Network Optimization: Models, Methods, and Solutions T2 - Optimization Methods and Software Y1 - 2015 U6 - https://doi.org/10.1080/10556788.2014.888426 SN - 1055-6788 SN - 1029-4937 VL - 30 IS - 1 SP - 15 EP - 53 ER - TY - GEN A1 - Fügenschuh, Armin A1 - Geißler, Björn A1 - Gollmer, Ralf A1 - Hayn, Christine A1 - Henrion, René A1 - Hiller, Benjamin A1 - Humpola, Jesco A1 - Koch, Thorsten A1 - Lehmann, Thomas A1 - Martin, Alexander A1 - Mirkov, Radoslava A1 - Morsi, Antonio A1 - Rövekamp, Jessica A1 - Schewe, Lars A1 - Schmidt, Martin A1 - Schultz, Rüdiger A1 - Schwarz, Robert A1 - Schweiger, Jonas A1 - Stangl, Claudia A1 - Steinbach, Marc C. A1 - Willert, Bernhard M. T1 - Mathematical Optimization for Challenging Network Planning Problems in Un- bundled Liberalized Gas Markets T2 - Energy Systems Y1 - 2014 U6 - https://doi.org/10.1007/s12667-013-0099-8 SN - 1868-3975 SN - 1868-3967 VL - 5 IS - 3 SP - 449 EP - 473 ER - TY - GEN A1 - Peter, Sebastian A1 - Karich, Alexander A1 - Ullrich, René A1 - Gröbe, Glenn A1 - Scheibner, Katrin A1 - Hofrichter, Martin T1 - Enzymatic one-pot conversion of cyclohexane into cyclohexanone: Comparison of four fungal peroxygenases T2 - Journal of Molecular Catalysis : B, Enzymatic N2 - Unspecific peroxygenases (UPO; EC 1.11.2.1) represent a group of secreted heme-thiolate proteins that are capable of catalyzing the mono-oxygenation of diverse organic compounds, using only H2O2 as a co-substrate. Here we show that the four peroxygenases AaeUPO, MroUPO, rCciUPO and rNOVO catalyze the stepwise hydroxylation of cyclohexane to cyclohexanol and cyclohexanone. The catalytic efficiencies (kcat/Km) for the initial hydroxylation were in the same order of magnitude for all four peroxygenases (∼104 M−1 s−1), whereas they differed in the second step. The conversion of cyclohexanol by AaeUPO and rCciUPO was 1–2 orders of magnitude less efficient (∼102 M−1 s−1) than by MroUPO and rNOVO (∼104 M−1 s−1). The highest conversion rate in terms of H2O2 utilization was accomplished by MroUPO under repeated addition of the peroxide (87% in relation to the total products formed). Using the latter UPO, we successfully established a micro-mixing reaction device (SIMM-V2) for the oxidation of cyclohexane. As cyclohexanone is a chemical of high relevance, for example, as starting material for polymer syntheses or as organic solvent, new enzymatic production pathways for this compound are of interest to complement existing chemical and biotechnological approaches. Stable and versatile peroxygenases, as those presented here, may form a promising biocatalytic platform for the development of such enzyme-based processes. KW - cyclohexane KW - cyclohexanol KW - cyclohexanone KW - UPO KW - Peroxygenase Y1 - 2014 UR - http://www.sciencedirect.com/science/article/pii/S138111771300266X U6 - https://doi.org/10.1016/j.molcatb.2013.09.016 IS - 103 SP - 47 EP - 51 ER - TY - GEN A1 - Steffens, S. A1 - Becker, C. A1 - Amkreutz, Daniel A1 - Klossek, André A1 - Kittler, Martin A1 - Chen, Y.-Y. A1 - Schnegg, Alexander A1 - Klingsporn, Max A1 - Abou-Ras, Daniel A1 - Lips, Klaus A1 - Rech, Bernd T1 - Impact of Dislocations and Dangling Bond Defects on The Electrical Performance of Crystalline Silicon Thin Films T2 - Applied Physics Letters Y1 - 2014 U6 - https://doi.org/10.1063/1.4890625 SN - 1077-3118 SN - 0003-6951 VL - 105 IS - 2 SP - 022108 ER - TY - GEN A1 - Reiche, Manfred A1 - Kittler, Martin A1 - Pippel, Eckhard A1 - Übensee, Hartmut A1 - Kosina, Hans A1 - Grill, Alexander A1 - Stanojevic, Zlatan A1 - Baumgartner, Oskar T1 - Impact of Defect-Induced Strain on Device Properties T2 - Advanced Engineering Materials Y1 - 2017 U6 - https://doi.org/10.1002/adem.201600736 SN - 1527-2648 N1 - Art.-Nr. 1600736 VL - 19 IS - 8 ER - TY - GEN A1 - Karich, Alexander A1 - Scheibner, Katrin A1 - Ullrich, René A1 - Hofrichter, Martin T1 - Exploring the catalase activity of unspecific peroxygenases and the mechanism of peroxide-dependent heme destruction T2 - Journal of Molecular Catalysis B: Enzymatic N2 - The catalase activity of three unspecific peroxygenases (UPOs) from the agaric basidiomycetes Agrocybe aegerita, Coprinopsis cinerea and Marasmius rotula was investigated. The study included analysis of pH dependency of the catalase reaction and H₂O₂ mediated enzyme inactivation as well as experiments on the influence of a second substrate on the course of catalase reaction. Apparent kinetic parameters (Km, kcat) for the catalase activity of UPOs were determined. Inactivation of UPOs by H₂O₂ is discussed with regard to O₂ production and remaining UPO activity. Furthermore formation of biliverdin as heme destruction product was demonstrated along with the formation of UPO compound III as a possible intermediate that forces the destruction process. Radical trapping experiments with methyl benzoate gave indication for the formation of hydroxyl radicals in the presence of excess H₂O₂. Eventually, a plausible pathway of heme destruction has been proposed, proceeding via UPO compound III and subsequent hydroxyl radical formation, which in turn may cause heme bleaching and verdoheme and biliverdin formation. KW - Peroxygenase Y1 - 2016 UR - http://www.sciencedirect.com/science/article/pii/S1381117716302090 U6 - https://doi.org/10.1016/j.molcatb.2016.10.014 SN - 1381-1177 VL - 134 IS - A SP - 238 EP - 246 ER - TY - GEN A1 - Schulz, Christian M. A1 - Skrzypczak, M. A1 - Schneider, Erich A1 - Hapfelmeier, Alexander A1 - Martin, J. A1 - Kochs, Eberhard F. A1 - Schneider, G. T1 - Assessment of subjective workload in an anaesthesia simulator environment: reliability and validity T2 - European Journal of Anaesthesiology Y1 - 2011 SN - 0265-0215 VL - 28 IS - 7 SP - 502 EP - 505 ER - TY - GEN A1 - Karich, Alexander A1 - Ullrich, René A1 - Scheibner, Katrin A1 - Hofrichter, Martin T1 - Fungal unspecific peroxygenases oxidize the majority of organic EPA priority pollutants T2 - Frontiers in Microbiology N2 - Unspecific peroxygenases (UPOs) are secreted fungal enzymes with promiscuity for oxygen transfer and oxidation reactions. Functionally, they represent hybrids of P450 monooxygenases and heme peroxidases; phylogenetically they belong to the family of heme-thiolate peroxidases. Two UPOs from the basidiomycetous fungi Agrocybe aegerita (AaeUPO) and Marasmius rotula (MroUPO) converted 35 out of 40 compounds listed as EPA priority pollutants, including chlorinated benzenes and their derivatives, halogenated biphenyl ethers, nitroaromatic compounds, polycyclic aromatic hydrocarbons (PAHs) and phthalic acid derivatives. These oxygenations and oxidations resulted in diverse products and-if at all-were limited for three reasons: (i) steric hindrance caused by multiple substitutions or bulkiness of the compound as such (e.g., hexachlorobenzene or large PAHs), (ii) strong inactivation of aromatic rings (e.g., nitrobenzene), and (iii) low water solubility (e.g., complex arenes). The general outcome of our study is that UPOs can be considered as extracellular counterparts of intracellular monooxygenases, both with respect to catalyzed reactions and catalytic versatility. Therefore, they should be taken into consideration as a relevant biocatalytic detoxification and biodegradation tool used by fungi when confronted with toxins, xenobiotics and pollutants in their natural environments. KW - Peroxygenase Y1 - 2017 UR - https://www.frontiersin.org/articles/10.3389/fmicb.2017.01463/full U6 - https://doi.org/10.3389/fmicb.2017.01463 SN - 1664-302X VL - 8 ER -