TY - GEN A1 - Hertel, Robert A1 - Schöne, Kerstin A1 - Mittelstädt, Carolin A1 - Meißner, Janek A1 - Zschoche, Nick A1 - Collignon, Madeline A1 - Kohler, Christian A1 - Friedrich, Ines A1 - Schneider, Dominik A1 - Hoppert, Michael A1 - Kuhn, Ramona A1 - Schwedt, Inge A1 - Scholz, Patricia A1 - Poehlein, Anja A1 - Martienssen, Marion A1 - Ischebeck, Till A1 - Daniel, Rolf A1 - Commichau, Fabian M. T1 - Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution T2 - Environmental Microbiology Reports N2 - Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate-resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate-sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia. Y1 - 2022 U6 - https://doi.org/10.1111/1758-2229.13022 SN - 1758-2229 VL - 14 IS - 1 SP - 70 EP - 84 ER - TY - GEN A1 - Kuhn, Ramona A1 - Jensch, Robert A1 - Fischer, Thomas A1 - Keuler, Klaus A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Sunlight Degradation of the Aminophosphonate Diethylenetriamine Penta-(Methylenephosphonic Acid) T2 - Solar N2 - Aminophosphonate diethylenetriamine penta(methylenephosphonic acid) (DTPMP) is a scale inhibitor commonly used in several industries. DTPMP is suspected to cause anthropogenic pollution through discharge into the aquatic environment. DTPMP is assumed to be degraded by sunlight radiation. We recently predicted a preliminary degradation pathway of DTPMP applyingUV treatment. Currently, we have not yet evidenced that DTPMP shows the same degradation pattern with natural sunlight. One major reason leads to the fact that the light spectrum emitted by UV lamps does not completely represent the natural sunlight spectrum, and the emitted UVB and UVA irradiation flux is much higher than for solar light. For that reason, the degradation pattern and kinetics might be different between artificial UV treatment and natural sunlight treatment. Here, we investigated whether DTPMP is degradable under natural sunlight radiation, and whether the degradation mechanisms determined through UV treatment are transferable to sunlight. We investigated five different treatment conditions, i.e., DTPMP degradation in direct or diffuse sunlight, in diffuse sunlight with addition of Ca2+ or Mg2+, and in diffuse sunlight with local TW. Our experiment was carried out from March 2021 to October 2021. We performed LC/MS analyses and measured the release of o-PO4 3  . DTPMP was degraded with all five treatment conditions. The fastest DTPMP degradation occurred in direct and diffuse sunlight without addition of bivalent cations. The addition of Ca2+ and Mg2+ resulted in inhibited degradation. Similar effects occurred for sunlight treatment with local TW. We evidenced different degradation mechanisms for DTPMP depending on the presence of alkaline earth metals as we previously proposed for UV-treated DTPMP. However, both degradation mechanisms of DTPMP belong to the same degradation pathway determined with UV treatment. Therefore, we conclude that DTPMP undergoes a similar degradation pathway in sunlight as compared to UV light. KW - Photochemistry Y1 - 2022 U6 - https://doi.org/10.3390/solar2020009 SN - 2673-9941 VL - 2 IS - 2 SP - 141 EP - 157 ER - TY - GEN A1 - Sarpong, Daniella A1 - Amankwaa, Gordon A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - A novel biomethane (BMP) and somposting (CMP) potential framework for determining biogas and composting potential of urban organic waste T2 - Environmental Technology Y1 - 2022 U6 - https://doi.org/10.1080/09593330.2022.2145241 SN - 1479-487X VL - 45(2024) IS - 8 SP - 1471 EP - 1482 ER - TY - GEN A1 - Okofo, Louis Boansi A1 - Martienssen, Marion T1 - A three-dimensional numerical groundwater flow model to assess the feasibility of managed aquifer recharge in the Tamne River basin of Ghana T2 - Hydrogeology Journal Y1 - 2022 U6 - https://doi.org/10.1007/s10040-022-02492-7 SN - 1431-2174 VL - 30 IS - 4 SP - 1071 EP - 1090 ER - TY - GEN A1 - Okofo, Louis Boansi A1 - Adonadaga, Melvin-Guy A1 - Martienssen, Marion T1 - Groundwater age dating using multi-environmental tracers (SF6, CFC-11, CFC-12, δ18O, and δD) to investigate groundwater residence times and recharge processes in Northeastern Ghana T2 - Journal of Hydrology Y1 - 2022 U6 - https://doi.org/10.1016/j.jhydrol.2022.127821 SN - 1879-2707 SN - 0022-1694 VL - Vol. 610 SP - 1 EP - 18 ER - TY - GEN A1 - Okofo, Louis Boansi A1 - Bedu-Addo, Kenneth A1 - Martienssen, Marion T1 - Characterization of groundwater in the 'Tamnean' Plutonic Suite aquifers using hydrogeochemical and multivariate statistical evidence: a study in the Garu-Tempane District, Upper East Region of Ghana T2 - Applied Water Science N2 - The 'Tamnean' Plutonic Suite aquifer is the main public water supply for the Garu-Tempane District. Thus, hydrogeochemical characterization is essential to provide valuable insights into pollution sources and the main controls on groundwater chemistry. In this regard, multivariate statistical methods, conventional hydrochemical graphical methods, and various ionic ratios complemented with PHREEQC geochemical modelling were carried out using 38 groundwater samples collected from the Tamnean Plutonic Suite aquifers, Ghana. The ionic ratio plots, the chloro-alkaline indices, and the graphical diagrams indicate that the major sources of groundwater chemistry are silicate mineral dissolution and cation exchange coupled with the leaching of domestic solid waste and nitrogen-based fertilizers. The Q-mode hierarchical cluster analysis reveals three spatial groundwater zones. Groundwater from recharge areas consists of Ca–Na–HCO3 water types in cluster 1. The intermediate zone is characterized by Ca–Mg–Na–HCO3 water types of moderate ionic compositions in cluster 2, and this evolves into a discharge zone in cluster 3 mainly of Ca–Mg–Na–HCO3–NO3 water types. The principal component analysis (PCA) reveals three factors, which account for 81% of the total variance, and this suggests most of the groundwater chemistry had longer interaction with the lithological materials. The PHREEQC geochemical modelling consisting of mineral saturation index indicates that groundwater is mostly supersaturated with respect to dolomite and undersaturated with respect to calcite, anhydrite, fluorite, gypsum, and halite. Based on the water quality index, the groundwater in the district is generally suitable for drinking water purposes. All the samples are within the World Health Organization acceptable limits for drinking water except for lower pH, elevated nitrate and bromide concentrations in some of the wells. About 10.5% of the groundwater samples are contaminated with nitrate, which may pose a health danger to the inhabitants in the communities. The finding of this study will not only contribute to solving the research paucity regarding the Tamnean Plutonic Suite aquifers in the Garu-Tempane District but will serve as a valuable document for water managers and decision-makers in Ghana. KW - Tamnean Plutonic Suite KW - Groundwater chemistry KW - Saturation index KW - Elevated nitrate concentrations KW - Engineering Y1 - 2022 U6 - https://doi.org/10.1007/s13201-021-01559-2 SN - 2190-5487 SN - 2190-5495 VL - 12 IS - 2 PB - Springer International Publishing ER -