TY - GEN A1 - Sowa, Mandy A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology T2 - Clinical Reviews in Allergy & Immunology Y1 - 2017 U6 - https://doi.org/10.1007/s12016-016-8574-3 SN - 1559-0267 SN - 1080-0549 VL - 53 IS - 1 SP - 87 EP - 104 ER - TY - GEN A1 - Sowa, Mandy A1 - Reddig, Annika A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.02 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Liedtke, Victoria A1 - Rose, Laura A1 - Hiemann, Rico A1 - Nasser, Abdullah A1 - Rödiger, Stefan A1 - Bonaventura, Alena A1 - Winkler, Laura A1 - Sowa, Mandy A1 - Stöckle, Michael A1 - Schierack, Peter A1 - Junker, Kerstin A1 - Roggenbuck, Dirk T1 - Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence? T2 - International Journal of Molecular Sciences N2 - Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence. KW - LEDGF/p75 KW - autoimmunity KW - CRISPR/Cas9 KW - dsDNA KW - mDNA Y1 - 2023 U6 - https://doi.org/10.3390/ijms24076166 SN - 1422-0067 VL - 24 IS - 7 ER -