TY - GEN A1 - Scholz, Juliane A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Sowa, Mandy A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Bogdanos, Dimitrios Petrou A1 - Meroni, Pier Luigi A1 - Radice, Antonella A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2015 U6 - https://doi.org/10.1515/cclm-2015-0083 SN - 1437-4331 VL - 53 IS - 12 SP - 1991 EP - 2002 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Röber, Nadja A1 - Anderer, Ursula A1 - Csernok, Elena A1 - Bogdanos, Dimitrios Petrou A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA T2 - PLoS ONE KW - ANCA KW - vasculitis KW - screening KW - confirmatory testing KW - multiplexing KW - CytoBead technology Y1 - 2014 U6 - https://doi.org/10.1371/journal.pone.0107743 SN - 1932-6203 VL - 9 IS - 9 ER - TY - GEN A1 - Schlör, Anja A1 - Holzlöhner, Pamela A1 - Listek, Martin A1 - Grieß, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Füner, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk A1 - Hanack, Katja T1 - Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2 T2 - New Biotechnology Y1 - 2018 U6 - https://doi.org/10.1016/j.nbt.2018.03.006 SN - 1876-4347 SN - 1871-6784 VL - 45 SP - 60 EP - 68 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Sowa, Mandy A1 - Kolenda, Rafał A1 - Baumgart, Daniel C. A1 - Pratschke, Johann A1 - Papp, Maria A1 - Tornai, Tamas A1 - Suchanski, Jaroslaw A1 - Bogdanos, Dimitrios Petrou A1 - Mytilinaiou, Maria G. A1 - Hammermann, Jutta A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Schramm, Christoph A1 - Franke, Andre A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype T2 - Frontiers in Immunology Y1 - 2018 U6 - https://doi.org/10.3389/fimmu.2018.01959 SN - 1664-3224 VL - 9 IS - 1959 ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Hiemann, Rico T1 - Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen T2 - Journal of Laboratory Medicine Y1 - 2014 U6 - https://doi.org/10.1515/labmed-2014-0041 VL - 38 IS - 6 SP - 309 EP - 317 ER - TY - GEN A1 - Sowa, Mandy A1 - Trezzi, Barbara A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Somma, Valentina A1 - Sinico, Renato Alberto A1 - Roggenbuck, Dirk A1 - Radice, Antonella T1 - Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis T2 - Medicine Y1 - 2016 U6 - https://doi.org/10.1097/MD.0000000000005225 SN - 0025-7974 VL - 95 IS - 44 SP - 5225 ER - TY - GEN A1 - Sowa, Mandy A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology T2 - Clinical Reviews in Allergy & Immunology Y1 - 2017 U6 - https://doi.org/10.1007/s12016-016-8574-3 SN - 1559-0267 SN - 1080-0549 VL - 53 IS - 1 SP - 87 EP - 104 ER - TY - GEN A1 - Sowa, Mandy A1 - Reddig, Annika A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.02 SN - 2519-9005 VL - 3 ER -