TY - CHAP A1 - Ziems, Christian A1 - Tannert, Daniel A1 - Tillmann, Christine A1 - Krüger, Perco A1 - Krause, S. A1 - Krautz, Hans Joachim T1 - Untersuchungen zur fortschrittlichen alkalischen Druckelektrolyse am Wasserstoff-Forschungszentrum Cottbus Y1 - 2011 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Krause, Christian A1 - Novotny, Ivan A1 - Splonskowski, Markus A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Hummel, Helmut A1 - Wolfbeis, Otto S. T1 - Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry T2 - Materials Science Forum KW - Biosensor KW - Capacitance KW - Chemical Sensors KW - Electrode Y1 - 1998 U6 - https://doi.org/10.4028/www.scientific.net/MSF.287-288.423 SN - 1662-9752 VL - 287-288 SP - 423 EP - 426 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Mass, Markus A1 - Krause, Christian A1 - Wolfbeis, Otto S. T1 - Capacitive approach to determine phospholipase A2 activity towards artificial and natural substrates T2 - Analytical Chemistry N2 - A capacitive approach has been employed to develop a novel method to determine phospholipase activity. The sensing electrodes have a structure like Au/S(CH₂)₁₇CH₃/substrate/electrolyte. Hydrolysis of the substrate, mediated by phospholipase A₂, leads to the formation of water-soluble products from the insoluble substrate. This results in desorption of these products into aqueous phase and corresponding increase of the electrode capacitance. The requirement of high water solubility of the reaction products can be achieved in two ways. In the first, short-chain phospholipids are used as the substrate, in which case, water-soluble products are formed and no additional reagents are required to promote desorption of these products. The sensors prepared by this strategy provide sensitive qualitative detection of phospholipases. The second way is based on the use of a water-soluble acceptor (for example, β-cyclodextrin) to solubilize the products of hydrolysis. It allows semiquantitative detection of phospholipase activity toward long-chain natural substrates. The reaction kinetics for this case was found to be monoexponential and linearly dependent on the phospholipase concentration. The detection limit of this method, as tested with phospholipase A₂ from bee venom and soy bean lecithin as the substrate, is ∼0.5 ng/mL (500 μunits/mL). Y1 - 1998 U6 - https://doi.org/10.1021/ac980102w SN - 0003-2700 SN - 1520-6882 VL - 70 IS - 17 SP - 3674 EP - 3678 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Krause, Christian A1 - Heckmann, Klaus Dietrich T1 - Capacitive detection of adsorption/desorption on hydrophobized gold electrodes: an application for surfactants adsorption and phospholipase assay T2 - Chimica Chronica, New Series Y1 - 1996 VL - 25 SP - 179 EP - 194 ER - TY - GEN A1 - Krause, Christian A1 - Mirsky, Vladimir M. A1 - Heckmann, Klaus Dietrich T1 - Capacitive detection of surfactant adsorption on hydrophobized gold electrodes T2 - Langmuir N2 - Nonspecific adsorption of surfactants on polycrystalline gold electrodes, covered by a chemically adsorbed monomolecular layer of octadecanethiol, was studied by means of capacitive measurements. Homologous series of fatty acids (from decane to tetradecane acids) and dodecanepyridinium chloride were used as surfactants. The behavior of the electrode was completely different in the first and subsequent cycles of adsorption/desorption. In the first adsorption cycle, an initial capacitance jump at low concentrations (<10 μM) occurs, while later the curves show the usual saturation behavior. The first surfactant layer could not be completely desorbed; only the electrode capacitance shown after the jump was reached. Subsequent cycles do not reveal any capacitance jumps and were completely reversible. Adsorption curves in the series of monoalkyl acids are shifted according to the Traube rule. A comparison with the surfactant adsorption at the air/water interface has been done. Y1 - 1996 U6 - https://doi.org/10.1021/la9601869 SN - 0743-7463 VL - 12 IS - 25 SP - 6059 EP - 6064 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Krause, Christian A1 - Heckmann, Klaus Dietrich T1 - Capacitive sensor for lipolytic enzymes T2 - Thin Solid Films N2 - The preparation of the capacitive biosensor for monitoring the activity of enzymes (lipases, phospholipases, etc.) hydrolysing waterinsoluble substrates into water-soluble products is described. The sensor is based on a sandwich-like structure: Au/S(CH₂)₁₇CH₁/substrate/electrolyte. Hydrolysis of the substrate leads to the formation of water-soluble products and desorption of these compounds from the electrode. When the product formation is the rate-limiting step of this process, the desorption rate is determined by the enzyme activity. The desorption can be easily monitored as an increase of the electrode capacitance. By following this principle it was possible to develop a sensor monitoring phospholipase A₂ activity even at the 50 pg ml-1 level. KW - Phospholipase AZ KW - Enzyme assay KW - Capacitive sensor KW - Self-assembly layer Y1 - 1996 U6 - https://doi.org/10.1016/S0040-6090(95)08485-1 SN - 0040-6090 VL - 284-285 SP - 939 EP - 941 ER - TY - CHAP A1 - Krause, Chris A1 - Meinberg, Uwe A1 - Krebs, Irene A1 - Schlauer, Christian A1 - Otto, Dierk T1 - A Contribution To SPDM Strategies In Aerospace T2 - NAFEMS SPDM World Congress Stockholm 2017, Summary of proceedings N2 - Long product lifecycles are standard in aerospace, thus simulation data must be handled. This data is critical because it represents real value for the enterprise. Furthermore, simulation data is created in lengthy business processes and often ends in large files. This sets challenges for handling it to allow an efficient storage approach but also full traceability. This paper is an interim result of the research projects VITIV (project number: 80164702) and the ProFIT-Programme supported by the federal state of Brandenburg and the European Union. Initially, the status quo must be analysed. Therefore, the current development processes to develop engine parts were investigated. The focus is the data created by analysts but for this task, all input data created prior must be known, handled and stored as well. Additionally, a new modular data structure is developed to fulfil the aforementioned requirements. However, two concepts for storing must be compared. The first would be to store all input files and boundary conditions separately without storing a full executable simulation file. This approach requires the functionality to automatically rebuild the executable file which takes time but provides a lean and modular storage. The other method would be to store the large runnable file and avoid protracted processes to rebuild the file. In some cases such rebuilding could last for several weeks. In this case, traceability must be secured. Currently, not all data regarding CAE is stored in the PLM system. The reasons differ from constraints in terms of configuration of the system, as well as a lack in foresight. This means that some process actors are focussed on a fast way to store their data but do not take into consideration that these objects must be found and used for investigations in the future. The next step will be to work on the process automation. These workflows should reduce the amount of manual user interactions, hence to speed up the processes and avoid sources of error. Furthermore, the developed method for storing the data has to reach the next level: from the secured test environment into a pre-production system. Y1 - 2017 SN - 978-1-910643-37-2 ER - TY - GEN A1 - Barth, Maximilian A1 - Werner, Magdalena A1 - Otto, Pascal A1 - Richwien, Benjamin A1 - Bahramsari, Samira A1 - Krause, Maximilian A1 - Schwan, Benjamin A1 - Abendroth, Christian T1 - Microwave-assisted organic acids and green hydrogen production during mixed culture fermentation T2 - Biotechnology for Biofuels and Bioproducts N2 - Background: The integration of anaerobic digestion into bio-based industries can create synergies that help render anaerobic digestion self-sustaining. Two-stage digesters with separate acidification stages allow for the production of green hydrogen and short-chain fatty acids, which are promising industrial products. Heat shocks can be used to foster the production of these products, the practical applicability of this treatment is often not addressed sufficiently, and the presented work therefore aims to close this gap. Methods: Batch experiments were conducted in 5 L double-walled tank reactors incubated at 37 °C. Short microwave heat shocks of 25 min duration and exposure times of 5–10 min at 80 °C were performed and compared to oven heat shocks. Pairwise experimental group differences for gas production and chemical parameters were determined using ANOVA and post–hoc tests. High-throughput 16S rRNA gene amplicon sequencing was performed to analyse taxonomic profiles. Results: After heat–shocking the entire seed sludge, the highest hydrogen productivity was observed at a substrate load of 50 g/l with 1.09 mol H2/mol hexose. With 1.01 mol H2/mol hexose, microwave-assisted treatment was not significantly different from oven-based treatments. This study emphasised the better repeatability of heat shocks with microwave-assisted experiments, revealing low variation coefficients averaging 29%. The pre-treatment with microwaves results in a high predictability and a stronger microbial community shift to Clostridia compared to the treatment with the oven. The pre-treatment of heat shocks supported the formation of butyric acid up to 10.8 g/l on average, with a peak of 24.01 g/l at a butyric/acetic acid ratio of 2.0. Conclusion: The results support the suitability of using heat shock for the entire seed sludge rather than just a small inoculum, making the process more relevant for industrial applications. The performed microwave-based treatment has proven to be a promising alternative to oven-based treatments, which ultimately may facilitate their implementation into industrial systems. This approach becomes economically sustainable with high-temperature heat pumps with a coefficient of performance (COP) of 4.3. Y1 - 2024 U6 - https://doi.org/10.1186/s13068-024-02573-7 SN - 2731-3654 VL - 17 IS - 1 PB - Springer Science and Business Media LLC ER - TY - GEN A1 - Eisenhauer, Nico A1 - Frank, Karin A1 - Weigelt, Alexandra A1 - Bartkowski, Bartosz A1 - Beugnon, Rémy A1 - Liebal, Katja A1 - Mahecha, Miguel A1 - Quaas, Martin A1 - Al‐Halbouni, Djamil A1 - Bastos, Ana A1 - Bohn, Friedrich J. A1 - Brito, Mariana Madruga de A1 - Denzler, Joachim A1 - Feilhauer, Hannes A1 - Fischer, Rico A1 - Fritsche, Immo A1 - Guimaraes‐Steinicke, Claudia A1 - Hänsel, Martin A1 - Haun, Daniel B. M. A1 - Herrmann, Hartmut A1 - Huth, Andreas A1 - Kalesse‐Los, Heike A1 - Koetter, Michael A1 - Kolleck, Nina A1 - Krause, Melanie A1 - Kretschmer, Marlene A1 - Leitão, Pedro J. A1 - Masson, Torsten A1 - Mora, Karin A1 - Müller, Birgit A1 - Peng, Jian A1 - Pöhlker, Mira L. A1 - Ratzke, Leonie A1 - Reichstein, Markus A1 - Richter, Solveig A1 - Rüger, Nadja A1 - Sánchez‐Parra, Beatriz A1 - Shadaydeh, Maha A1 - Sippel, Sebastian A1 - Tegen, Ina A1 - Thrän, Daniela A1 - Umlauft, Josefine A1 - Wendisch, Manfred A1 - Wolf, Kevin A1 - Wirth, Christian A1 - Zacher, Hannes A1 - Zaehle, Sönke A1 - Quaas, Johannes T1 - A belowground perspective on the nexus between biodiversity change, climate change, and human well‐being T2 - Journal of Sustainable Agriculture and Environment N2 - Soil is central to the complex interplay among biodiversity, climate, and society. This paper examines the interconnectedness of soil biodiversity, climate change, and societal impacts, emphasizing the urgent need for integrated solutions. Human‐induced biodiversity loss and climate change intensify environmental degradation, threatening human well‐being. Soils, rich in biodiversity and vital for ecosystem function regulation, are highly vulnerable to these pressures, affecting nutrient cycling, soil fertility, and resilience. Soil also crucially regulates climate, influencing energy, water cycles, and carbon storage. Yet, climate change poses significant challenges to soil health and carbon dynamics, amplifying global warming. Integrated approaches are essential, including sustainable land management, policy interventions, technological innovations, and societal engagement. Practices like agroforestry and organic farming improve soil health and mitigate climate impacts. Effective policies and governance are crucial for promoting sustainable practices and soil conservation. Recent technologies aid in monitoring soil biodiversity and implementing sustainable land management. Societal engagement, through education and collective action, is vital for environmental stewardship. By prioritizing interdisciplinary research and addressing key frontiers, scientists can advance understanding of the soil biodiversity–climate change–society nexus, informing strategies for environmental sustainability and social equity. Y1 - 2024 U6 - https://doi.org/10.1002/sae2.12108 SN - 2767-035X VL - 3 IS - 2 PB - Wiley ER - TY - GEN A1 - Otto, Pascal A1 - Witkabel, Philipp A1 - Barth, Maximilian A1 - Ammar, Aziz Ben A1 - Rocktaeschel, Benjamin A1 - Torrent, Daniel A1 - Latorre-Peréz, Adriel A1 - Krause, Maximilian A1 - Abendroth, Christian T1 - Adaptation of the anaerobic microbiome for in-situ power-to-CH4 processes through fuzzy logic control of H2 input T2 - Bioresource technology reports N2 - This study presents the first application of fuzzy logic control (FLC) for regulating in-situ biological methanation via H2 injection in a fixed-bed reactor. Given the role of CH4 as a storable and infrastructure-compatible energy carrier, the aim was to improve CH4 production by dynamically adapting the H2 supply to the metabolic capacity of the microbiome. This will pave the way for the biological utilisation and storage of renewable H2. Over a 226- day operational period, H2 input was gradually increased based on real-time headspace gas measurements. Coupling the gradual increase in H2 supply with real-time gas composition data allowed the microbiome to adapt dynamically to changing process conditions. This approach led to a 49 % increase in CH4 concentration, from 58.4 % to 87.0 %, and improved H2 conversion rates from 98.2 % to 99.0 %. Chemical analyses of COD, organic acids and nitrogen were performed, as well as taxonomic analyses using 16S rRNA sequencing, and multivariate methods were applied to confirm the adaptation of the microbiome to the FLC H2 injection. The adopted microbial community was dominated by strictly hydrogenotrophic methanogens, Methanoculleus and Methanobacterium, while increased abundances of Petrimonas, Rectinema, Syntrophomonas, and Geobacter indicate interspecies H2 transfer and syntrophic cooperation enhancing methanogenesis. These findings demonstrate that FLC-based H2 control enables dynamic adjustment of in-situ methanation, optimising the interaction between hydrogenogenic, fermentative, syntrophic acetate-oxidising, and hydrogenotrophic microorganisms. This work introduces an adaptive control strategy that supports stable and efficient bio-CH4 production and represents a significant advance in the field of power-to-gas technologies. KW - In-situ methanation KW - Biological hydrogen methanation KW - Power-to-Gas KW - Adaptively evolved microbiome KW - 16S rRNA sequencing KW - Fuzzy logic control KW - Multivariate analysis Y1 - 2025 U6 - https://doi.org/10.1016/j.biteb.2025.102194 SN - 2589-014X VL - 31 SP - 1 EP - 11 PB - Elsevier BV CY - Amsterdam ER -