TY - GEN A1 - Gerk, U. A1 - Franke, Ralf-Peter A1 - Jung, Ernst Michael A1 - Scheller, B. A1 - Krüger-Genge, Anne A1 - Jung, Friedrich T1 - Imaging of coronary arteries using ionic versus non-ionic radiographic contrast media: Intraindividual comparison study T2 - Clinical Hemorheology and Microcirculation Y1 - 2019 U6 - https://doi.org/10.3233/CH-199217 SN - 1875-8622 ER - TY - GEN A1 - Jung, Friedrich A1 - Krüger-Genge, Anne A1 - Waldeck, Peter A1 - Küpper, Jan-Heiner T1 - Spirulina platensis, a super food? T2 - Journal of Cellular Biotechnology N2 - Spirulina platensis, a multicelluar, photosynthetic prokaryote (algae) contains a high amount of proteins, vitamins and minerals superior to many foods as e.g. soybeans. Thus, Spirulina platensis was recognized as nutritious food by the United Nations World Food Conference. Due to the high amount of nutritive ingredients Spirulina has a long history as dietary supplement. In addition, spirulina platensis is also efficiently used as forage with known effects on flesh, egg and plumage color, milk yield and fertility. The versatile utilization of the alga can be explained on the one hand with the nutrient levels and on the other hand with recognized effects as anti-viral, anti-bacterial, anti-oxidant, anti-diabetic, anti-cancer and anti-inflammatory substance. Therefore, this alga is named as “superfood”. Beyond, these algae convert carbon dioxide into organic substances and produce oxygen during their growth in alkaline and saline water thereby not wasting fresh water allowing the production in barren areas. Despite this diverse use of Spirulina platensis due to its beneficial properties, many basic mechanisms on a molecular and cellular level are not well understood and should be explored in future studies. KW - Spirulina KW - health effects KW - dietary supplements KW - liver protection KW - virus infection KW - HIV KW - nutrition KW - animal feeding Y1 - 2019 UR - https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb189012 U6 - https://doi.org/10.3233/JCB-189012 SN - 2352-3689 SN - 2352-3697 VL - 5 IS - 1 SP - 43 EP - 54 ER - TY - GEN A1 - Jung, Ernst Michael A1 - Moran, Valentina Ocañaa A1 - Engel, Martin A1 - Krüger-Genge, Anne A1 - Stroszczynski, Christian A1 - Jung, Friedrich T1 - Modified contrast-enhanced ultrasonography with the new high-resolution examination technique of high frame rate contrast-enhanced ultrasound (HiFR-CEUS) for characterization of liver lesions: First results T2 - Clinical Hemorheology and Microcirculation Y1 - 2022 U6 - https://doi.org/10.3233/CH-221449 SN - 1875-8622 SP - 1 EP - 16 ER - TY - GEN A1 - Jung, Friedrich A1 - Braune, Steffen A1 - Jung, Conrad H. G. A1 - Krüger-Genge, Anne A1 - Waldeck, Peter A1 - Petrick, Ingolf A1 - Küpper, Jan-Heiner T1 - Lipophilic and Hydrophilic Compounds from Arthrospira platensis and Its Effects on Tissue and Blood Cells—An Overview T2 - Life Y1 - 2022 U6 - https://doi.org/10.3390/life12101497 SN - 2075-1729 VL - 12 IS - 10 ER - TY - GEN A1 - Küpper, Jan-Heiner A1 - Krüger-Genge, Anne A1 - Jung, Friedrich T1 - 40th conference of the German society for clinical microcirculation and hemorheology (DGKMH) T2 - Clinical Hemorheology and Microcirculation Y1 - 2021 U6 - https://doi.org/10.3233/ch-219100 SN - 1875-8622 SN - 1386-0291 VL - 79 IS - 1 SP - 1 EP - 2 ER - TY - GEN A1 - Schulz, Christian A1 - Krüger-Genge, Anne A1 - Jung, Friedrich A1 - Lendlein, Andreas T1 - Aptamer supported in vitro endothelialization of poly(ether imide) films T2 - Clinical Hemorheology and Microcirculation N2 - Implantation of synthetic small-diameter vascular bypass grafts is often associated with an increased risk of failure, due to thrombotic events or late intimal hyperplasia. As one of the causes an insufficient hemocompatibility of the artificial surface is discussed. Endothelialization of synthetic grafts is reported to be a promising strategy for creating a self-renewing and regulative anti-thrombotic graft surface. However, the establishment of a shear resistant cell monolayer is still challenging. In our study, cyto- and immuno-compatible poly(ether imide) (PEI) films were explored as potential biomaterial for cardiovascular applications. Recently, we reported that the initial adherence of primary human umbilical vein endothelial cells (HUVEC) was delayed on PEI-films and about 9 days were needed to establish a confluent and almost shear resistant HUVEC monolayer. To accelerate the initial adherence of HUVEC, the PEI-film surface was functionalized with an aptamer-cRGD peptide based endothelialization supporting system. With this functionalization the initial adherence as well as the shear resistance of HUVEC on PEI-films was considerable improved compared to the unmodified polymer surface. The in vitro results confirm the general applicability of aptamers for an efficient functionalization of substrate surfaces. KW - aptamer KW - endothelialization KW - physical functionalization KW - poly(ether imide) films KW - shear resistance Y1 - 2020 U6 - https://doi.org/10.3233/CH-190775 SN - 1875-8622 SN - 1386-0291 VL - 75 IS - 2 SP - 201 EP - 217 ER - TY - GEN A1 - Lau, S. A1 - Rangarajan, R. A1 - Krüger-Genge, Anne A1 - Braune, Steffen A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Effects of acrolein in comparison to its prodrug cyclophosphamide on human primary endothelial cells in vitro T2 - Toxicology in Vitro N2 - Cyclophosphamide (CPA) is one of the most successful anticancer prodrugs that becomes effective after biotransformation in the liver resulting in the toxic metabolite acrolein. Cancer is often accompanied by thromboembolic events, which might be a result of dysfunctional endothelial cells due to CPA treatment. Here, the effect of 1 mM CPA or acrolein (10/50/100/500 μM) on human umbilical vein endothelial cells (HUVECs) was analyzed after two days of treatment. The addition of CPA or 10 μM acrolein did not affect HUVECs. However, concentrations of 100 μM and 500 μM acrolein significantly reduced the number of adherent cells by 86 ± 13% and 99 ± 1% and cell viability by 51 ± 29% and 93 ± 8% compared to the control. Moreover, pronounced stress fibers as well as multiple nuclei were observed and von Willebrand factor (vWF) was completely released. Lactate dehydrogenase was 8.5 ± 7.0-fold and 252.9 ± 42.9-fold increased showing a loss of cell membrane integrity. The prostacyclin and thromboxane secretion was significantly increased by the addition of 500 μM acrolein (43.1 ± 17.6-fold and 246.4 ± 106.3-fold) indicating cell activation/pertubation. High doses of acrolein led to HUVEC death and loss of vWF production. This effect might be associated with the increased incidence of thromboembolic events in cancer patients treated with high doses of CPA. KW - Human umbilical venous endothelial cells KW - Acrolein KW - Aneuploidy KW - von Willebrand factor KW - in vitro study Y1 - 2020 U6 - https://doi.org/10.1016/j.tiv.2019.104685 SN - 0887-2333 VL - 62 ER - TY - GEN A1 - Lee, Seahyoung A1 - Ganesan, Ramakrishnan A1 - Krüger-Genge, Anne A1 - Kratz, Karl A1 - Franke, Ralf-Peter A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Substrate-enzyme affinity-based surface modification strategy for endothelial cell-specific binding under shear stress T2 - Clinical Hemorheology and Microcirculation N2 - Establishing an endothelial cell (EC) monolayer on top of the blood contacting surface of grafts is considered to be a promising approach for creating a hemocompatible surface. Here we utilized the high affinity interactions between the EC plasma membrane expressed enzyme called endothelin converting enzyme-1 (ECE-1) and its corresponding substrate big Endothelin-1 (bigET-1) to engineer an EC-specific binding surface. Since enzymatic cleavage of substrates require physical interaction between the enzyme and its corresponding substrate, it was hypothesized that a surface with chemically immobilized synthetic bigET-1 will preferentially attract ECs over other types of cells found in vascular system such as vascular smooth muscle cells (VSMCs). First, the expression of ECE-1 was significantly higher in ECs, and ECs processed synthetic bigET-1 to produce ET-1 in a cell number-dependent manner. Such interaction between ECs and synthetic bigET-1 was also detectible in blood. Next, vinyl-terminated self-assembled monolayers (SAMs) were established, oxidized and activated on a glass substrate as a model to immobilize synthetic bigET-1 via amide bonds. The ECs cultured on the synthetic bigET-1-immobilized surface processed larger amount of synthetic bigET-1 to produce ET-1 compared to VSMCs (102.9±5.13 vs. 9.75±0.74 pg/ml). The number of ECs bound to the synthetic bigET-1-immobilized surface during 1 h of shearing (5dyne/cm2) was approximately 3-fold higher than that of VSMCs (46.25±12.61 vs. 15.25±3.69 cells/100×HPF). EC-specific binding of synthetic bigET-1-immobilized surface over a surface modified with collagen, a common substance for cell adhesion, was also observed. The present study demonstrated that using the substrate-enzyme affinity (SEA) of cell type-specific enzyme and its corresponding substrate can be an effective method to engineer a surface preferentially binds specific type of cells. This novel strategy might open a new route toward rapid endothelialization under dynamic conditions supporting the long-term patency of cardiovascular implants. KW - endothelin converting enzyme-1 KW - big endothelin-1 KW - endothelialization KW - shear resistance Y1 - 2020 UR - https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch190736 U6 - https://doi.org/10.3233/CH-190736 SN - 1875-8622 VL - 75 IS - 1 SP - 85 EP - 98 ER -