TY - GEN A1 - Wetzel, Stefanie A1 - Allertz, Peter J. A1 - Koschnicke, Sören A1 - Tasso, Mariana A1 - Salchert, Katrin T1 - Immobilized enzymes – valuable tools for the indication of temperature events T2 - Biocatalysis and Biotransformation N2 - The enzymes trypsin and urease were covalently tethered to cellulose to utilize their ability to produce colored products as a consequence of enzymatic activity. Therefore, cellulose had to be chemically modified first in order to generate appropriate chemical functionalities. Different approaches including periodate supported oxidation followed by immobilization via reductive amination, insertion of a reactive polymer interface, and cross-linking inside the cellulose matrix were utilized for the immobilization. The success of immobilization was assessed by the quantification of surface-bound protein as well as by recording of enzymatic activities under different conditions. The enzymatic activity of trypsin and urease was maintained best when a hydrophilic intermediate polymer layer was used for immobilization. The applicability of immobilized enzymes as temperature indicators was demonstrated using cross-linked urease. KW - Cellulose KW - immobilization KW - trypsin KW - urease KW - reactive polymer KW - temperature label Y1 - 2013 U6 - https://doi.org/10.3109/10242422.2013.777433 SN - 1024-2422 SN - 1029-2446 VL - 31 IS - 2 SP - 100 EP - 113 ER -