TY - GEN A1 - Cígler, Petr A1 - Král, Vladimír A1 - Kožíšek, Milan A1 - Konvalinka, Jan A1 - Mirsky, Vladimir M. T1 - Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization? T2 - Colloids and Surfaces B: Biointerfaces N2 - Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices. KW - HIV protease KW - Protein adsorption KW - Protein-resistant surfaces KW - Self-assembled monolayer KW - Surface plasmon resonance (SPR) Y1 - 2008 U6 - https://doi.org/10.1016/j.colsurfb.2008.01.011 SN - 0927-7765 VL - 64 IS - 1 SP - 145 EP - 149 ER -