TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Wimonć, Marcjanna A1 - Aleksandrowicz, Adrianna A1 - Ali, Aamir A1 - Szabo, Istvan A1 - Tedin, Karsten A1 - Bartholdson, Scott J. A1 - Pickhard, Derek A1 - Schierack, Peter T1 - Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens T2 - Applied and Environmental Microbiology Y1 - 2021 U6 - https://doi.org/10.1128/AEM.02177-20 SN - 1098-5336 VL - 87 IS - 2 ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Szabo, M. A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin T2 - 5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016 Y1 - 2016 UR - https://www.asm.org/images/1903%20asm%20salmonella%20program%20web3.pdf SP - S. 125 PB - American Society for Microbiology CY - Washington, DC ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Sidorczuk, Katarzyna A1 - Becker, Juliane A1 - Aleksandrowicz, Adrianna A1 - Baraniewicz, Karolina A1 - Ludwig, Christina A1 - Ali, Aamir A1 - Kingsley, Robert A. A1 - Schierack, Peter A1 - Kolenda, Rafał T1 - Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity T2 - Microbiology Spectrum N2 - Escherichia coli intestinal infection pathotypes are characterized by distinct adhesion patterns, including the recently described clumpy adhesion phenotype. Here, we identify and characterize the genetic factors contributing to the clumpy adhesion of E. coli strain 4972. In this strain, the transcriptome and proteome of adhered bacteria were found to be distinct from planktonic bacteria in the supernatant. A total of 622 genes in the transcriptome were differentially expressed in bacteria present in clumps relative to the planktonic bacteria. Seven genes targeted for disruption had variable distribution in different pathotypes and nonpathogenic E. coli, with the pilV and spnT genes being the least frequent or absent from most groups. Deletion (Δ) of five differentially expressed genes, flgH, ffp, pilV, spnT, and yggT, affected motility, adhesion, or antibiotic stress. ΔflgH exhibited 80% decrease and ΔyggT depicted 184% increase in adhesion, and upon complementation, adhesion was significantly reduced to 13%. ΔflgH lost motility and was regenerated when complemented, whereas Δffp had significantly increased motility, and reintroduction of the same gene reduced it to the wild-type level. The clumps produced by Δffp and ΔspnT were more resistant and protected the bacteria, with ΔspnT showing the best clump formation in terms of ampicillin stress protection. ΔyggT had the lowest tolerance to gentamicin, where the antibiotic stress completely eliminated the bacteria. Overall, we were able to investigate the influence of clump formation on cell surface adhesion and antimicrobial tolerance, with the contribution of several factors crucial to clump formation on susceptibility to the selected antibiotics. KW - Infectious Diseases KW - Cell Biology KW - Microbiology (medical) KW - Genetics KW - General Immunology and Microbiology KW - Ecology KW - Physiology Y1 - 2024 U6 - https://doi.org/10.1128/spectrum.02606-23 SN - 2165-0497 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kolenda, Rafał A1 - Hiemann, Rico A1 - Berger, Enrico A1 - Grzymajlo, Krzysztof A1 - Swidsinski, Alexander A1 - Juretzek, Thomas A1 - Meissner, Dirk A1 - Mydlak, Karsten A1 - Reinhold, Dirk A1 - Nolan, Lisa K. A1 - Roggenbuck, Dirk T1 - Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2) T2 - Gut Y1 - 2015 U6 - https://doi.org/10.1136/gutjnl-2014-307854 SN - 1468-3288 SN - 0017-5749 VL - 64 IS - 3 SP - 517 EP - 519 ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Kolenda, Rafał A1 - Schierack, Peter A1 - Weinreich, Jörg A1 - Rödiger, Stefan A1 - Schierack, Jakob A1 - Stubbe, Michael A1 - Lkhagvasuren, Davaa A1 - Guenther, Sebastian A1 - Schaufler, Katharina T1 - Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia T2 - Microorganisms Y1 - 2021 U6 - https://doi.org/10.3390/microorganisms9020372 SN - 2076-2607 VL - 9 IS - 2 ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation T2 - Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM Y1 - 2017 PB - Spektrumverlag CY - Heidelberg ER - TY - GEN A1 - Grzymajlo, Krzysztof A1 - Ugorski, Maciej A1 - Suchanski, Jaroslaw A1 - Kedzierska, Anna E. A1 - Kolenda, Rafał A1 - Jarzab, Anna A1 - Biernatowska, Agnieszka A1 - Schierack, Peter T1 - The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity T2 - Frontiers in Cellular and Infection Microbiology Y1 - 2017 U6 - https://doi.org/10.3389/fcimb.2017.00326 SN - 2235-2988 VL - 7 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schierack, Peter T1 - A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli T2 - Frontiers in Cellular and Infection Microbiology Y1 - 2015 U6 - https://doi.org/10.3389/fcimb.2015.00023 SN - 2235-2988 VL - 5 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Rödiger, Stefan A1 - Sauer, Lysann A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Weinreich, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Gerber, Ulrike A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation T2 - Frontiers in Microbiology Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01905 SN - 1664-302X VL - 9 IS - 1905 ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Ali, Aamir A1 - Kolenda, Rafał A1 - Olowe, Olugbenga Adekunle A1 - Weinreich, Jörg A1 - Li, Ganwu A1 - Schierack, Peter T1 - The role of AJB35136 and fdtA genes in biofilm formation by avian pathogenic Escherichia coli T2 - BMC Veterinary Research Y1 - 2023 U6 - https://doi.org/10.1186/s12917-023-03672-7 SN - 1746-6148 VL - 19 ER -