TY - GEN A1 - Steinbrecht, Susanne A1 - Pfeifer, Nadine A1 - Herzog, Natalie A1 - Katzenberger, Nadine A1 - Schulz, Christian A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells T2 - Toxicology Letters N2 - Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min-1 x mg-1 protein analyzed by phenacetin conversion. Both clones showed dramatically increased sensitivity to the hepatotoxic compound aflatoxin B1 (EC50<100 nM) when compared to parental HepG2 cells (EC50 ∼5 μM). Thus, newly established cell lines are an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP1A2. KW - HepG2 KW - CYP1A2 KW - Cytochrome P450 KW - Liver metabolism Y1 - 2020 U6 - https://doi.org/10.1016/j.toxlet.2019.11.006 SN - 0378-4274 VL - 319 SP - 155 EP - 159 ER - TY - GEN A1 - Herzog, Natalie A1 - Katzenberger, Nadine A1 - Martin, Frank A1 - Schmidtke, Kai-Uwe A1 - Küpper, Jan-Heiner T1 - Generation of cytochrome P450 3A4-overexpressing HepG2 cell clones for standardization of hepatocellular testosterone 6β-hydroxylation activity T2 - Journal of Cellular Biotechnology Y1 - 2015 U6 - https://doi.org/10.3233/JCB-15002 SN - 2352-3697 SN - 2352-3689 VL - 1 IS - 1 SP - 15 EP - 26 ER -