TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Lopens, Steffi A1 - Krawczyk, Marcin A1 - Papp, Maria A1 - Milkiewicz, Piotr A1 - Schierack, Peter A1 - Liu, Yudong A1 - Wunsch, Ewa A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00129-x SN - 2038-3274 SN - 2038-0305 VL - 11 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Elmont, Emilien A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Boucraut, Joseph T1 - Autoimmune Perpheral Neuropathies and Contribution of Antiganglioside/Sulphatide Autoantibody Testing T2 - Mediterranean Jounal of Rheumatology Y1 - 2020 U6 - https://doi.org/10.31138/mjr.31.1.10 SN - 2529-198X VL - 31 IS - 1 SP - 10 EP - 18 ER - TY - GEN A1 - Roggenbuck, Johannes J. A1 - Zarske, Grit A1 - Schierack, Peter A1 - Wunderlich, Gerd A1 - Conrad, Karsten A1 - Kotzerke, Joerg A1 - Roggenbuck, Dirk A1 - Zöphel, Klaus T1 - Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) - one step less, similar results? T2 - Nuklearmedizin Y1 - 2021 U6 - https://doi.org/10.1055/a-1277-5972 SN - 0029-5566 VL - 60 IS - 1 SP - 38 EP - 46 ER - TY - GEN A1 - Lopens, Steffi A1 - Wunsch, Ewa A1 - Milkiewicz, Malgorzata A1 - Röber, Nadja A1 - Zarske, Grit A1 - Nasser, Abdullah A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Rödiger, Stefan A1 - Krawczyk, Marcin A1 - Roggenbuck, Dirk A1 - Milkiewicz, Piotr T1 - PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis T2 - Diagnostics N2 - A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease. KW - primary sclerosing cholangitis KW - antibodies against serine protease proteinase-3 KW - survival KW - health-related quality of life KW - liver biochemistry KW - disease severity Y1 - 2022 U6 - https://doi.org/10.3390/diagnostics12112682 SN - 2075-4418 VL - 12 IS - 11 ER - TY - GEN A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Sack, Ulrich A1 - Anderer, Ursula A1 - Conrad, Karsten T1 - Die HEp-2-Zelle als Target für multiparametrische Autoantikörperanalytik – Automatisierung und Standardisierung/The HEp-2 cell as target for multiparametric autoantibody analyses: automation and standardisation T2 - Journal of Laboratory Medicine N2 - Trotz Einführung von Multiplex-Assays ist die Bestimmung von Autoantikörpern (AAK) mittels indirekter Immunfluoreszenz (IIF) nach wie vor ein wesentlicher Bestandteil der Autoimmundiagnostik. Das Screening auf nichtorganspezifische AAK wird in der Routinediagnostik von autoimmunen rheumatischen Erkrankungen mittels indirekter Immunfluoreszenz an HEp-2-Zellen durchgeführt. Leider differieren die mit diesem Test erhaltenen Befunde z. T. recht erheblich auf Grund von objektiven (z. B. HEp-2-Zellfixierung) und subjektiven (z. B. Erfahrungswerte) Faktoren. Die Intra- und Interlaborvarianzen sind daher relativ hoch. Erste Ergebnisse von Evaluierungsstudien eines neu entwickelten Systems zur automatischen Bildanalyse (AKLIDES®) zeigen einen Weg in Richtung Standardisierung (bzw. Harmonisierung) des HEp-2-Zell-Assays auf, in dem zunächst subjektive Fehlinterpretationen vermieden werden können. Eine weitere Harmonisierung erfordert die Weiterentwicklung der Software-Algorithmen zur Mustererkennung sowie neue Kalibrationssysteme. KW - Autoantikörper KW - Automatisierung KW - HEp-2 KW - Immunfluoreszenz Y1 - 2011 U6 - https://doi.org/10.1515/JLM.2011.053 SN - 1439-0477 VL - 35 IS - 6 SP - 351 EP - 361 ER -