TY - GEN A1 - Nowak, Elisabeth A1 - Martin, F. A1 - George, Sandra A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Küpper, Jan-Heiner T1 - Bacterial production and purification of immunoreactive paraneoplastic neurological syndrome autoantigen Ma2 T2 - Journal of Cellular Biotechnology N2 - Paraneoplastic neurological syndromes (PNS) are caused by an immune response against neuronal proteins upon their ectopical expression in tumor cells. Ma2 belongs to the protein family of paraneoplastic Ma antigens (PNMA). Detection of Ma2-specific autoantibodies is relevant for diagnostics of anti-Ma2 PNS and an underlying tumor such as germ testicular cancer, small cell lung cancer or breast cancer. Thus, early tumor treatment should improve the outcome for PNS therapy either. Dot blot immunoassay based on recombinantly expressed and purified autoantigens could offer a sensitive method for identification of paraneoplastic autoantibodies from sera of PNS patients. Here we present purification with IMAC and FPLC of human Ma2 autoantigen upon its recombinant expression in E.coli. Furthermore, we provide evidence that dot blot immunoassays with purified Ma2 autoantigen can be used for detection of Ma2-specific autoantibodies from sera of PNS patients. KW - Autoimmune disease KW - autoantibodies KW - dot blot immunoassay KW - FPLC KW - IMAC KW - Ma2 KW - PNMA2 KW - PNS KW - T7 expression system Y1 - 2017 UR - https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb15029 U6 - https://doi.org/10.3233/JCB-15029 SN - 2352-3697 VL - 2 IS - 2 SP - 85 EP - 91 ER - TY - BOOK A1 - Paumen, Anja A1 - Küpper, Jan-Heiner T1 - It’s the planet, stupid! Sieben Perspektiven zum Klimawandel : mit Interviewbeiträgen von Hartmut Graßl, Hans-Werner Sinn, Ernst-Ulrich von Weizsäcker, Meinhard Miegel, Mathias Binswanger, Enoch zu Guttenberg und Wulf Schiefenhövel Y1 - 2015 SN - 978-3-86581-739-6 SN - 3-86581-739-4 PB - oekom Verlag CY - München ER - TY - PAT A1 - Küpper, Jan-Heiner T1 - Vorrichtung und Verfahren zur Sequestrierung von atmosphärischen Kohlendioxid Y1 - 2017 N1 - EU-Patentanmeldung ER - TY - CHAP A1 - George, Sandra A1 - Noack, Monika A1 - Vanek, Monika A1 - Rentzsch, Juliane A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Küpper, Jan-Heiner T1 - Expression of nicotinic acetylcholine receptor subunits in HEp-2 cells for immunodetection of autoantibody specificities in sera from Myasthenia gravis patients T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - 195 EP - 196 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Krüger-Genge, Anne A1 - Steinbrecht, Susanne A1 - Küpper, Jan-Heiner A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Evidence for cytostatic effect of cyclophosphamide on human vein endothelial cells in cancer therapy: preliminary in vitro results. T2 - Clinical Hemorheology and Microcirculation Y1 - 2018 U6 - https://doi.org/10.3233/CH-189125 SN - 1875-8622 SN - 1386-0291 VL - 69 IS - 1-2 SP - 267 EP - 276 ER - TY - GEN A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - Human Hepatocyte Systems for in vitro Toxicology Analysis. T2 - Journal of Cellular Biotechnology Y1 - 2018 U6 - https://doi.org/10.3233/JCB-179012 SN - 2352-3697 SN - 2352-3689 VL - 3 IS - 2 SP - 85 EP - 93 ER - TY - GEN A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - Optimized protocol for induction of cytochrome P450 enzymes 1A2 and 3A4 in human primary-like hepatocyte cell strain HepaFH3 to study in vitro toxicology T2 - Clinical Hemorheology and Microcirculation Y1 - 2018 U6 - https://doi.org/10.3233/CH-189321 SN - 1386-0291 SN - 1875-8622 VL - 70 IS - 4 SP - 563 EP - 571 ER - TY - GEN A1 - Steinbrecht, Susanne A1 - König, Rosalie A1 - Schmidtke, Kai-Uwe A1 - Herzog, Natalie A1 - Scheibner, Katrin A1 - Krüger-Genge, Anne A1 - Jung, Friedrich A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - Metabolic activity testing can underestimate acute drug cytotoxicity as revealed by HepG2 cell clones overexpressing cytochrome P450 2C19 and 3A4 T2 - Toxicology Y1 - 2018 U6 - https://doi.org/10.1016/j.tox.2018.11.008 SN - 0300-483X VL - 412 SP - 37 EP - 47 ER - TY - GEN A1 - Krüger-Genge, Anne A1 - Braune, Steffen A1 - Walter, Maria A1 - Kratz, Karl A1 - Küpper, Jan-Heiner A1 - Krengel, M. A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Influence of surface treatments of poly(n-butyl acrylate) networks on fibroblasts adhesion, morphology and viability T2 - Clinical Hemorheology and Microcirculation Y1 - 2018 U6 - https://doi.org/10.3233/CH-189130 SN - 1875-8622 SN - 1386-0291 VL - 69 IS - 1-2 SP - 305 EP - 316 ER - TY - GEN A1 - Nowak, Elisabeth A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - ATP-based cell viability assay is superior to trypan blue exclusion and XTT assay in measuring cytotoxicity of anticancer drugs Taxol and Imatinib, and proteasome inhibitor MG-132 on human hepatoma cell line HepG2. T2 - Clinical Hemorheology and Microcirculation Y1 - 2018 U6 - https://doi.org/10.3233/CH-189120 SN - 1386-0291 SN - 1875-8622 VL - 69 IS - 1-2 SP - 327 EP - 336 ER - TY - GEN A1 - Hofman, Jakub A1 - Sorf, Ales A1 - Vagiannis, Dimitrios A1 - Sucha, Simona A1 - Novotna, Eva A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner A1 - Ceckova, Martina A1 - Staud, Frantisek T1 - Interactions of Alectinib with Human ATP-Binding Cassette Drug Efflux Transporters and Cytochrome P450 Biotransformation Enzymes: Effect on Pharmacokinetic Multidrug Resistance T2 - Drug Metabolism and Disposition Y1 - 2019 U6 - https://doi.org/10.1124/dmd.119.086975 SN - 1521-009X SN - 0090-9556 VL - 47 IS - 7 SP - 699 EP - 709 ER - TY - GEN A1 - Steinbrecht, Susanne A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - HepG2 cells with recombinant cytochrome P450 enzyme overexpression: Their use and limitation as in vitro liver model T2 - Journal of Cellular Biotechnology Y1 - 2019 U6 - https://doi.org/10.3233/JCB-189013 SN - 2352-3697 SN - 2352-3689 VL - 5 IS - 1 SP - 55 EP - 64 ER - TY - GEN A1 - Hofman, Jakub A1 - Sorf, Ales A1 - Vagiannis, Dimitrios A1 - Sucha, Simona A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner A1 - Chen, Si A1 - Guo, Lei A1 - Ceckova, Martina A1 - Staud, Frantisek T1 - Brivanib Exhibits Potential for Pharmacokinetic Drug-Drug Interactions and the Modulation of Multidrug Resistance through the Inhibition of Human ABCG2 Drug Efflux Transporter and CYP450 Biotransformation Enzymes T2 - Molecular Pharmaceutics Y1 - 2019 U6 - https://doi.org/10.1021/acs.molpharmaceut.9b00361 VL - 16 IS - 11 SP - 4436 EP - 4450 ER - TY - GEN A1 - Haileka, Vanessa A1 - George, Sandra A1 - Steinbrecht, Susanne A1 - Jung, Friedrich A1 - Reinehr, R. A1 - Küpper, Jan-Heiner T1 - Colon cancer cells cultured under hyperosmotic conditions as in vitro model to investigate dehydration effects on cancer drug susceptibility T2 - Clinical Hemorheology and Microcirculation Y1 - 2019 U6 - https://doi.org/10.3233/CH-199210 SN - 1875-8622 VL - 73 IS - 1 SP - 169 EP - 176 ER - TY - GEN A1 - Schulz, Christian A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - NADPH-cytochrome P450 reductase expression and enzymatic activity in primary-like human hepatocytes and HepG2 cells for in vitro biotransformation studies T2 - Clinical Hemorheology and Microcirculation Y1 - 2019 U6 - https://doi.org/10.3233/CH-199226 SN - 1386-0291 VL - 73 IS - 1 SP - 249 EP - 260 ER - TY - GEN A1 - Uhlig, Katja A1 - Gehre, Christian P. A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner A1 - Coleman, D. A1 - Püschel, G. A1 - Duschl, Claus T1 - Real-time monitoring of oxygen consumption of hepatocytes in a microbioreactor T2 - Toxicology Letters Y1 - 2018 U6 - https://doi.org/10.1016/j.toxlet.2018.06.652 SN - 0378-4274 VL - 295 IS - Supplement 1 SP - S115 ER - TY - GEN A1 - Steinbrecht, Susanne A1 - Pfeifer, Nadine A1 - Herzog, Natalie A1 - Katzenberger, Nadine A1 - Schulz, Christian A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner T1 - HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells T2 - Toxicology Letters N2 - Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min-1 x mg-1 protein analyzed by phenacetin conversion. Both clones showed dramatically increased sensitivity to the hepatotoxic compound aflatoxin B1 (EC50<100 nM) when compared to parental HepG2 cells (EC50 ∼5 μM). Thus, newly established cell lines are an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP1A2. KW - HepG2 KW - CYP1A2 KW - Cytochrome P450 KW - Liver metabolism Y1 - 2020 U6 - https://doi.org/10.1016/j.toxlet.2019.11.006 SN - 0378-4274 VL - 319 SP - 155 EP - 159 ER - TY - GEN A1 - Lau, S. A1 - Rangarajan, R. A1 - Krüger-Genge, Anne A1 - Braune, Steffen A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner A1 - Lendlein, Andreas A1 - Jung, Friedrich T1 - Effects of acrolein in comparison to its prodrug cyclophosphamide on human primary endothelial cells in vitro T2 - Toxicology in Vitro N2 - Cyclophosphamide (CPA) is one of the most successful anticancer prodrugs that becomes effective after biotransformation in the liver resulting in the toxic metabolite acrolein. Cancer is often accompanied by thromboembolic events, which might be a result of dysfunctional endothelial cells due to CPA treatment. Here, the effect of 1 mM CPA or acrolein (10/50/100/500 μM) on human umbilical vein endothelial cells (HUVECs) was analyzed after two days of treatment. The addition of CPA or 10 μM acrolein did not affect HUVECs. However, concentrations of 100 μM and 500 μM acrolein significantly reduced the number of adherent cells by 86 ± 13% and 99 ± 1% and cell viability by 51 ± 29% and 93 ± 8% compared to the control. Moreover, pronounced stress fibers as well as multiple nuclei were observed and von Willebrand factor (vWF) was completely released. Lactate dehydrogenase was 8.5 ± 7.0-fold and 252.9 ± 42.9-fold increased showing a loss of cell membrane integrity. The prostacyclin and thromboxane secretion was significantly increased by the addition of 500 μM acrolein (43.1 ± 17.6-fold and 246.4 ± 106.3-fold) indicating cell activation/pertubation. High doses of acrolein led to HUVEC death and loss of vWF production. This effect might be associated with the increased incidence of thromboembolic events in cancer patients treated with high doses of CPA. KW - Human umbilical venous endothelial cells KW - Acrolein KW - Aneuploidy KW - von Willebrand factor KW - in vitro study Y1 - 2020 U6 - https://doi.org/10.1016/j.tiv.2019.104685 SN - 0887-2333 VL - 62 ER - TY - GEN A1 - Jung, Friedrich A1 - Krüger-Genge, Anne A1 - Waldeck, Peter A1 - Küpper, Jan-Heiner T1 - Spirulina platensis, a super food? T2 - Journal of Cellular Biotechnology N2 - Spirulina platensis, a multicelluar, photosynthetic prokaryote (algae) contains a high amount of proteins, vitamins and minerals superior to many foods as e.g. soybeans. Thus, Spirulina platensis was recognized as nutritious food by the United Nations World Food Conference. Due to the high amount of nutritive ingredients Spirulina has a long history as dietary supplement. In addition, spirulina platensis is also efficiently used as forage with known effects on flesh, egg and plumage color, milk yield and fertility. The versatile utilization of the alga can be explained on the one hand with the nutrient levels and on the other hand with recognized effects as anti-viral, anti-bacterial, anti-oxidant, anti-diabetic, anti-cancer and anti-inflammatory substance. Therefore, this alga is named as “superfood”. Beyond, these algae convert carbon dioxide into organic substances and produce oxygen during their growth in alkaline and saline water thereby not wasting fresh water allowing the production in barren areas. Despite this diverse use of Spirulina platensis due to its beneficial properties, many basic mechanisms on a molecular and cellular level are not well understood and should be explored in future studies. KW - Spirulina KW - health effects KW - dietary supplements KW - liver protection KW - virus infection KW - HIV KW - nutrition KW - animal feeding Y1 - 2019 UR - https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb189012 U6 - https://doi.org/10.3233/JCB-189012 SN - 2352-3689 SN - 2352-3697 VL - 5 IS - 1 SP - 43 EP - 54 ER - TY - GEN A1 - Krüger-Genge, Anne A1 - Jung, Friedrich A1 - Küpper, Jan-Heiner A1 - Lehmann, Christian A1 - Franke, Ralf-Peter T1 - Actin type and distribution in erythrocytes T2 - Journal of Cellular Biotechnology N2 - Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation. KW - Erythrocytes KW - echinocytes KW - submembraneous cytoskeleton KW - actin Y1 - 2018 UR - https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb179014 U6 - https://doi.org/10.3233/JCB-179014 SN - 2352-3697 SN - 2352-3689 VL - 3 IS - 2 SP - 81 EP - 83 ER -