TY - GEN A1 - Kuhn, Ramona A1 - Böllmann, Jörg A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Data on DNA gel sample load, gel electrophoresis, PCR and cost analysis T2 - Data in Brief N2 - The data presented in this article provide supporting information to the related research article “Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples” (revised manuscript submitted to J. Microbiol. Methods). In that article, we compared the suitability of ten selected DNA extraction methods based on DNA quality, purity, quantity and applicability to universal PCR. Here we provide the data on the specific DNA gel sample load, all unreported gel images of crude DNA and PCR results, and the complete cost analysis for all tested extraction procedures and in addition two commercial DNA extraction kits for soil and water. KW - Cost analysis KW - DNA sample load KW - Gel electrophoresis Y1 - 2018 U6 - https://doi.org/10.1016/j.dib.2017.11.082 SN - 2352-3409 VL - Vol. 16 SP - 732 EP - 751 ER - TY - GEN A1 - Engelbrecht, Steffen A1 - Mozooni, Mohammad A1 - Rathsack, Kristina A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Effect of increasing salinity to adapted and non-adapted Anammox biofilms T2 - Environmental Technology N2 - The Anammox process is an efficient low energy alternative for the elimination of nitrogen from wastewater. The process is already in use for side stream applications. However, some industrial wastewaters, e.g. from textile industry are highly saline. This may be a limit for the application of the Anammox process. The aim of this study was to evaluate the effects of different NaCl concentrations on the efficiency of adapted and non-adapted Anammox biofilms. The tested NaCl concentrations ranged from 0 to 50 g NaCl*L⁻¹. Concentrations below 30 g NaCl*L⁻¹did not significantly result in different nitrogen removal rates between adapted and non-adapted bacteria. However, adapted bacteria were significantly more resilient to salt at higher concentrations (40 and 50g NaCl*L⁻¹). The IC50 for adapted and non-adapted Anammox bacteria were 19.99 and 20.30 g NaCl*L⁻¹, respectively. Whereas adapted biomass depletes the nitrogen in ratios of NO-₂/NH+₄ around 1.20 indicating a mainly Anammox-driven consumption of the nitrogen, the ratio increases to 2.21 at 40 g NaCl*L⁻¹ for non-adapted biomass. This indicates an increase of other processes like denitrification. At lower NaCL concentrations up to 10 g NaCl*L⁻¹, a stimulating effect of NaCl to the Anammox process has been observed. KW - Adapted KW - Anammox KW - Biofilm KW - Non-adapted KW - Salinity Y1 - 2019 U6 - https://doi.org/10.1080/09593330.2018.1455748 SN - 0959-3330 SN - 1479-487X VL - 40 IS - 22 SP - 2880 EP - 2888 ER - TY - GEN A1 - Böllmann, Jörg A1 - Engelbrecht, Steffen A1 - Martienssen, Marion T1 - Autofluorescent characteristics of Candidatus Brocadia fulgida and the consequences for FISH and microscopic detection T2 - Systematic and Applied Microbiology N2 - An enrichment culture of Candidatus Brocadia fulgida was identified by three independent methods: analysis of autofluorescence using different microscope filter blocks and a fluorescence spectrometer, fluorescence in situ hybridization (FISH) with anammox-specific probes and partial sequencing of the 16S rDNA, hydrazine synthase hzsA and hydrazine oxidoreductase hzo. The filter block BV-2A (400–440, 470 LP, Nikon) was suitable for preliminary detection of Ca. B. fulgida. An excitation-emission matrix revealed three pairs of excitation-emission maxima: 288–330 nm, 288–478 nm and 417–478 nm. Several autofluorescent cell clusters could not be stained with DAPI or by FISH, suggesting empty but intact cells (ghost cells) or inhibited permeability. Successful staining of autofluorescent cells with the FISH probes Ban162 and Bfu613, even at higher formamide concentrations, suggested insufficient specificity of Ban162. Under certain conditions, Ca. B. fulgida lost its autofluorescence, which reduced the reliability of autofluorescence for identification and detection. Non-fluorescent Ca. Brocadia cells could not be stained with Ban162, but with Bfu613 at higher formamide concentrations, suggesting a dependency between both parameters. The phylogenetic analysis showed only good taxonomical clustering of the 16S rDNA and hzsA. In conclusion, careful consideration of autofluorescent characteristics is recommended when analysing and presenting FISH observations of Ca. B. fulgida to avoid misinterpretations and misidentifications. KW - 16S rDNA KW - Hydrazine synthase hzsA KW - Hydrazine oxidase hzo KW - FISH KW - Candidatus Kuenenia stuttgartiensi KW - Candidatus Brocadia fulgida Y1 - 2018 U6 - https://doi.org/10.1016/j.syapm.2018.09.002 SN - 1618-0984 SN - 0723-2020 VL - 42 IS - 2 SP - 135 EP - 144 ER - TY - GEN A1 - Kuhn, Ramona A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion A1 - Böllmann, Jörg T1 - DNA-Extraktion aus mikrobiellen Umweltproben T2 - BioSpektrum Y1 - 2018 U6 - https://doi.org/10.1007/s12268-018-0963-0 SN - 1868-6249 SN - 0947-0867 VL - 24 IS - 6 SP - 596 EP - 598 ER - TY - GEN A1 - Martienssen, Marion A1 - Böllmann, Jörg A1 - Nixdorf, Brigitte A1 - Rathsack, Kristina T1 - Calculation of hypolimnic denitrification in a dimictic freshwater lake during summer stratification T2 - Aquatic Microbial Ecology KW - Denitrification KW - freshwater Y1 - 2019 U6 - https://doi.org/10.3354/ame01911 SN - 0948-3055 SN - 1616-1564 VL - 83 IS - 2 SP - 189 EP - 201 ER - TY - GEN A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Comparison of different media for the detection of denitrifying and nitrate reducing bacteria in mesotrophic aquatic environments by the most probable number method T2 - Journal of Microbiological Methods N2 - The cultivation based characterization of microbial communities and the quantification of certain functional bacterial groups is still an essential part of microbiology and microbial ecology. For plate count methods meanwhile low strength media are recommended, since they cover a broader range of different species and result in higher counts compared to established high strength media. For liquid media, as they are used for most probable number (MPN) quantifications, comparisons between high and low strength media are rare. In this study we compare the performance of different high and low strength media for the MPN quantification of nitrate reducing and denitrifying bacteria in two different fresh water environments. We also calculated the cell specific turnover rates of several denitrifying cultures previously enriched in high and low strength media from three different fresh water environments and a waste water treatment plant. For fresh water samples, our results indicate that high strength media detect higher MPN of denitrifying bacteria and in equal MPN of nitrate reducing bacteria compared to low strength media, which is in contrary to plate count techniques. For sediment samples, high and low strength media performed equal. The cell specific turnover rate was independent from the enrichment media and the media of the performance test. The cause of the lower denitrifyer MPN in low strength media remains, however, unclear. The results are important for further MPN quantifications of bacteria in nutrient poor environments and for calculations of nitrogen turnover rates by kinetical models using the number of metabolic active cells as one parameter. Y1 - 2020 U6 - https://doi.org/10.1016/j.mimet.2019.105808 SN - 1872-8359 VL - 168 ER - TY - GEN A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions T2 - Biofouling N2 - The reuse of treated wastewater for irrigation is of increasing importance. The Ecosave farming project developed a new photocatalytic electrodialysis process for desalination and hygienization. However, membrane scaling significantly reduces filtration efficiency. This study investigated biofilm development on anion and cation exchange membranes at a wide pH range in pre-treated wastewater. Epifluorescence microscopic quantification of the biofilm by cell counts and surface coverage together with 16S rDNA gene copy numbers showed stronger biofilm development on the anion exchange membrane (AEM) compared with the cation exchange membrane (CEM) with up to 105 cells mm−2 and 20% surface coverage after three weeks. As the AEM biofilm developed best in neutral and a slightly alkaline pH, the CEM was colonized preferably at alkaline conditions. Extreme pH conditions strongly inhibited biofilm growth, which might help to minimize the maintenance effort by creating those conditions during the operation of the dialysis cell itself. Y1 - 2021 U6 - https://doi.org/10.1080/08927014.2021.1999424 SN - 1029-2454 VL - 37 IS - 9-10 SP - 998 EP - 1005 ER - TY - GEN A1 - Kuhn, Ramona A1 - Bryant, Isaac Mbir A1 - Jensch, Robert A1 - Böllmann, Jörg T1 - Applications of Environmental Nanotechnologies in Remediation, Wastewater Treatment, Drinking Water Treatment, and Agriculture T2 - Applied Nano N2 - Today, nanotechnologies (NTs) are well established in both private households and commercial markets. NTs are fully accepted in several sectors, such as medicine and pharmacy, and in industries, such as chemistry, electricity, food production, military, and other commercial branches, due to their unique properties. With regard to the growing demands for environmental resources caused by the still-growing global population, the application of NTs is an extremely important new branch in the environmental sector, delivering several advantages. Our review provides a comprehensive overview of the current developments in environmental remediation, wastewater treatment, drinking water treatment, and agriculture. More specifically, in the section on environmental remediation, we review the application of NTs towards enhanced reductive dechlorination, removal of heavy metals and remediation of oil spills. In the section on wastewater treatment, we highlight developments in the adsorption of heavy metals and persistent substances, advanced photocatalytic degradation of common wastewater pollutants, and improvements in membrane filtration processes. In the section on drinking water treatment, we discuss applications for the enhanced disinfection of pathogens, removal of heavy metals, point-of-use treatments, and the removal of organic matter. In the final section, on agriculture, we provide an overview of precision farming and the current state of the art concerning nanofertilisers, nanopesticides, nanoherbicides, and nano(bio)sensors. Y1 - 2022 UR - https://www.mdpi.com/2673-3501/3/1/5 U6 - https://doi.org/10.3390/applnano3010005 SN - 2673-3501 VL - 3 IS - 1 SP - 54 EP - 90 ER - TY - GEN A1 - Riedel, Ramona A1 - Krahl, Kathrin A1 - Buder, Kai A1 - Böllmann, Jörg A1 - Braun, Burga A1 - Martienssen, Marion T1 - Novel standard biodegradation test for synthetic phosphonates T2 - Journal of Microbiological Methods N2 - Determination of biodegradation of synthetic phosphonates such as aminotris(methylenephosphonic acid) (ATMP), ethylenediamine tetra(methylenephosphonic acid) (EDTMP), or diethylenetriamine penta(methylenephosphonic acid) (DTPMP) is a great challenge. Commonly, ready biodegradability of organic substances is assessed by OECD 301 standard tests. However, due to the chemical imbalance of carbon to phosphorus synthetic phosphonates do not promote microbial growth and, thus, limiting its biodegradation. Therefore, standard OECD test methods are not always reliable to predict the real biodegradability of phosphonates. In the presented study, we report the development of a standardized batch system suitable to synthetic phosphonates such as ATMP, EDTMP, DTPMP and others. The novel standard batch test is applicable with pure strains, activated sludge from different wastewater treatment plants (i.e., municipal and industrial), and with tap water as inoculum. We optimized the required calcium and magnesium exposure levels as well as the amount of the start inoculum biomass. We demonstrated that our test also allows to determine several parameters including ortho-phosphate (o-PO43􀀀 ), total phosphorus (TP), ammonium (NH4+) and total organic carbon (TOC). In addition, also LC/MS analyses of cell-free medium is applicable for determining the mother compounds and metabolites. We applied our optimized standardized batch with selected phosphonates and evidenced that the chemical structure has a major influence of the microbial growth rates. Thus, our novel batch test overcomes drawbacks of the OECD 301 test series for determination of easy biodegradability for stoichiometric imbalanced organic compounds such as phosphonates. Y1 - 2023 U6 - https://doi.org/10.1016/j.mimet.2023.106793 SN - 1872-8359 SN - 0167-7012 VL - Vol. 212 SP - 1 EP - 13 ER - TY - GEN A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - Glechoma hederacea (Lamiaceae) in North America: invasion history and current distribution T2 - Feddes Repertorium Y1 - 2004 U6 - https://doi.org/10.1002/fedr.200311035 SN - 0014-8962 SN - 1522-239X VL - 115 IS - 1-2 SP - 178 EP - 188 ER -