TY - GEN A1 - Ecke, Annemarie A1 - Scholka, Jenny A1 - Hansch, Anna A1 - Becker, Roland A1 - Anderer, Ursula T1 - Agar overlay technique vs. v-bottom plate: differentiation of human cartilage-like microtissues depend on plate format. T2 - Osteologie KW - microtissue KW - cartilage Y1 - 2019 U6 - https://doi.org/10.1055/s-0039-1680058 SN - 2567-5818 VL - 28 IS - 4 PB - Georg Thieme Verlag KG CY - Stuttgart ; New York ER - TY - GEN A1 - Ecke, Annemarie A1 - Scholka, Jenny A1 - Hansch, Anna A1 - Becker, Roland A1 - Anderer, Ursula T1 - Differentiation of human articular chondrocytes in microtissues depends on the cell aggregation technique KW - chondrogenic microtissues KW - differentiation KW - aggregation techniques Y1 - 2020 UR - https://www.ariabstracts.org/abstracts/2020/Collection1/c1.html ER - TY - GEN A1 - Anderer, Ursula A1 - Scholka, Jenny A1 - Hansch, Anna A1 - Becker, Roland A1 - Ecke, Annemarie T1 - Influence of the cell aggregation technique on the differentiation of human articular chondrocytes in microtissues T2 - Osteologie / Osteology KW - microtissue generation KW - chondrogenic differentiation KW - cell therapy KW - cartilage regeneration Y1 - 2022 U6 - https://doi.org/10.1055/s-0042-1755855 SN - 1019-1291 VL - 31 IS - 03 SP - 201 EP - 202 PB - Georg Thieme Verlag CY - Stuttgart ER - TY - GEN A1 - Ecke, Annemarie A1 - Lutter, Anne-Helen A1 - Scholka, Jenny A1 - Hansch, Anna A1 - Becker, Roland A1 - Anderer, Ursula T1 - Tissue Specific Differentiation of Human Chondrocytes Depends on Cell Microenvironment and Serum Selection T2 - Cells KW - Chondrocytes KW - Cartilage Y1 - 2019 U6 - https://doi.org/10.3390/cells8080934 SN - 2073-4409 VL - 8 IS - 8 ER - TY - GEN A1 - Ecke, Annemarie A1 - Scholka, Jenny A1 - Hansch, Anna A1 - Becker, Roland A1 - Anderer, Ursula T1 - Human serum or FCS: Differentiation of human cartilage-like microtissues depends on serum selection T2 - DGZ 2018 International Meeting of the German Society for Cell Biology: “Concepts of Cell Organisation and Dynamics” N2 - Introduction: Regeneration of articular cartilage defects is limited. Hence, cell-based therapies for the treatment were developed using e.g. chondrocytes isolated from patient biopsies. For cell culture fetal calf serum (FCS) is often used as media supplement to establish engineering processes for transplants in regenerative medicine. However, the usage of xenogeneic serum could mask the intrinsic behavior of human cells in vitro. This could be particularly important for the dedifferentiation process during cell expansion and redifferentiation in 3D-culture. The aim of this study was to compare the development of microtissues derived from chondrocytes cultivated in presence of FCS with those cultivated in human serum (HS). Material and Methods: Human articular chondrocytes isolated from knee joints were expanded in medium with HS or FCS. Scaffold-free microtissues were generated using the agar overlay technique. After four and eight weeks the macroscopic appearance (reflected-light microscopy) was documented and the size of the microtissues was determined. The differentiation degree was evaluated via histology to visualize typical glycosamino¬glycans (Safranin O, Alcian blue) and immunohisto¬chemistry (IH) to detect cartilage-specific markers (collagen type II, proteoglycans, COMP, Sox9) on cryosections. Results and Conclusion: Cells from all donors in both medium compositions generated microtissues – but with distinct differences in the differentiation levels. Microtissues cultured in medium with FCS showed a higher differentiation level compared to microtissues in HS. This is evidenced by a higher amount of matrix resulting in bigger microtissues (diameter in FCS is 42% larger compared to HS) and a more cartilage-like composition of the matrix (not any/little colouring in HS versus intense staining in FCS). IH analysis showed the same pattern. Not any/less positivity for collagen type II, proteoglycan, COMP, and Sox9 in HS versus moderate-to-high positivity in FCS-cultured microtissues. Absolute values vary from donor to donor whereas an individualized expression profile only became visible in microtissues cultured in HS medium. HS should be preferred for the engineering of cartilage-like microtissues, as it rather enables a "human-based" situation in vitro. Supported by „Gesundheitscampus Brandenburg“ and „Ministerium für Wissenschaft, Forschung und Kultur“, state of Brandenburg, Germany. KW - cartilage-like microtissues KW - serum type Y1 - 2018 UR - https://www.researchgate.net/publication/338863739_HUMAN_SERUM_OR_FCS_DIFFERENTIATION_OF_HUMAN_CARTILAGE-LIKE_MICROTISSUES_DEPENDS_ON_SERUM_SELECTION ER -