TY - GEN A1 - Buchanan, Erin M. A1 - Lewis, Savannah C. A1 - Paris, Bastien A1 - Forscher, Patrick S. A1 - Pavlacic, Jeffrey M. A1 - Beshears, Julie E. A1 - Drexler, Shira Meir A1 - Gourdon-Kanhukamwe, Amélie A1 - Mallik, Peter R A1 - Silan, Miguel Alejandro A. A1 - Miller, Jeremy K. A1 - IJzerman, Hans A1 - Moshontz, Hannah A1 - Beaudry, Jennifer L. A1 - Suchow, Jordan W. A1 - Chartier, Christopher R. A1 - Coles, Nicholas A. A1 - Sharifian, MohammadHasan A1 - Todsen, Anna Louise A1 - Levitan, Carmel A. A1 - Azevedo, Flávio A1 - Legate, Nicole A1 - Heller, Blake A1 - Rothman, Alexander J. A1 - Dorison, Charles A. A1 - Gill, Brian P. A1 - Wang, Ke A1 - Rees, Vaughan W. A1 - Gibbs, Nancy A1 - Goldenberg, Amit A1 - Thi Nguyen, Thuy-vy A1 - Gross, James J. A1 - Kaminski, Gwenaêl A1 - von Bastian, Claudia C. A1 - Paruzel-Czachura, Mariola A1 - Mosannenzadeh, Farnaz A1 - Azouaghe, Soufian A1 - Bran, Alexandre A1 - Ruiz-Fernandez, Susana A1 - Santos, Anabela Caetano A1 - Reggev, Niv A1 - Zickfeld, Janis H. A1 - Akkas, Handan A1 - Pantazi, Myrto A1 - Ropovik, Ivan A1 - Korbmacher, Max A1 - Arriaga, Patrícia A1 - Gjoneska, Biljana A1 - Warmelink, Lara A1 - Alves, Sara G. A1 - de Holanda Coelho, Gabriel Lins A1 - Stieger, Stefan A1 - Schei, Vidar A1 - Hanel, Paul H. P. A1 - Szaszi, Barnabas A1 - Fedotov, Maksim A1 - Antfolk, Jan A1 - Marcu, Gabriela-Mariana A1 - Schrötter, Jana A1 - Kunst, Jonas R. A1 - Geiger, Sandra J. A1 - Adetula, Adeyemi A1 - Kocalar, Halil Emre A1 - Kielińska, Julita A1 - Kačmár, Pavol A1 - Bokkour, Ahmed A1 - Galindo-Caballero, Oscar J. A1 - Djamai, Ikhlas A1 - Pöntinen, Sara Johanna A1 - AGESIN, Bamikole Emmanuel A1 - Jernsäther, Teodor A1 - Urooj, Anum A1 - Rachev, Nikolay R. A1 - Koptjevskaja-Tamm, Maria A1 - Kurfalı, Murathan A1 - Pit, Ilse L. A1 - Li, Ranran A1 - Çoksan, Sami A1 - Dubrov, Dmitrii A1 - Paltrow, Tamar Elise A1 - Baník, Gabriel A1 - Korobova, Tatiana A1 - Studzinska, Anna A1 - Jiang, Xiaoming A1 - Aruta, John Jamir Benzon R. A1 - Vintr, Jáchym A1 - Chiu, Faith A1 - Kaliska, Lada A1 - Berkessel, Jana B. A1 - Tümer, Murat A1 - Morales-Izquierdo, Sara A1 - Chuan-Peng, Hu A1 - Vezirian, Kevin A1 - Rosa, Anna Dalla A1 - Bialobrzeska, Olga A1 - Vasilev, Martin R. A1 - Beitner, Julia A1 - Kácha, Ondřej A1 - Žuro, Barbara A1 - Westerlund, Minja A1 - Nedelcheva-Datsova, Mina A1 - Findor, Andrej A1 - Krupić, Dajana A1 - Kowal, Marta A1 - Askelund, Adrian Dahl A1 - Pourafshari, Razieh A1 - Đorđević, Jasna Milošević A1 - Schmidt, Nadya-Daniela A1 - Baklanova, Ekaterina A1 - Szala, Anna A1 - Zakharov, Ilya A1 - Vranka, Marek A. A1 - Ihaya, Keiko A1 - Grano, Caterina A1 - Cellini, Nicola A1 - Białek, Michał A1 - Anton-Boicuk, Lisa A1 - Dalgar, Ilker A1 - Adıgüzel, Arca A1 - Verharen, Jeroen P. H. A1 - Maturan, Princess Lovella G. A1 - Kassianos, Angelos P. A1 - Oliveira, Raquel A1 - Čadek, Martin A1 - Adoric, Vera Cubela A1 - Özdoğru, Asil Ali A1 - Sverdrup, Therese E. A1 - Aczel, Balazs A1 - Zambrano, Danilo A1 - Ahmed, Afroja A1 - Tamnes, Christian K. A1 - Yamada, Yuki A1 - Volz, Leonhard A1 - Sunami, Naoyuki A1 - Suter, Lilian A1 - Vieira, Luc A1 - Groyecka-Bernard, Agata A1 - Kamburidis, Julia Arhondis A1 - Reips, Ulf-Dietrich A1 - Harutyunyan, Mikayel A1 - Adetula, Gabriel Agboola A1 - Allred, Tara Bulut A1 - Barzykowski, Krystian A1 - Antazo, Benedict G A1 - Zsido, Andras N. A1 - Šakan, Dušana Dušan A1 - Cyrus-Lai, Wilson A1 - Ahlgren, Lina Pernilla A1 - Hruška, Matej A1 - Vega, Diego A1 - Manunta, Efisio A1 - Mokady, Aviv A1 - Capizzi, Mariagrazia A1 - Martončik, Marcel A1 - Say, Nicolas A1 - Filip, Katarzyna A1 - Vilar, Roosevelt A1 - Staniaszek, Karolina A1 - Vdovic, Milica A1 - Adamkovic, Matus A1 - Johannes, Niklas A1 - Hajdu, Nandor A1 - Cohen, Noga A1 - Overkott, Clara A1 - Krupić, Dino A1 - Hubena, Barbora A1 - Nilsonne, Gustav A1 - Mioni, Giovanna A1 - Solorzano, Claudio Singh A1 - Ishii, Tatsunori A1 - Chen, Zhang A1 - Kushnir, Elizaveta A1 - Karaarslan, Cemre A1 - Ribeiro, Rafael R. A1 - Khaoudi, Ahmed A1 - Kossowska, Małgorzata A1 - Bavolar, Jozef A1 - Hoyer, Karlijn A1 - Roczniewska, Marta A1 - Karababa, Alper A1 - Becker, Maja A1 - Monteiro, Renan P. A1 - Kunisato, Yoshihiko A1 - Metin-Orta, Irem A1 - Adamus, Sylwia A1 - Kozma, Luca A1 - Czarnek, Gabriela A1 - Domurat, Artur A1 - Štrukelj, Eva A1 - Alvarez, Daniela Serrato A1 - Parzuchowski, Michal A1 - Massoni, Sébastien A1 - Czamanski-Cohen, Johanna A1 - Pronizius, Ekaterina A1 - Muchembled, Fany A1 - van Schie, Kevin A1 - Saçaklı, Aslı A1 - Hristova, Evgeniya A1 - Kuzminska, Anna O. A1 - Charyate, Abdelilah A1 - Bijlstra, Gijsbert A1 - Afhami, Reza A1 - Majeed, Nadyanna M. A1 - Musser, Erica D. A1 - Sirota, Miroslav A1 - Ross, Robert M. A1 - Yeung, Siu Kit A1 - Papadatou-Pastou, Marietta A1 - Foroni, Francesco A1 - Almeida, Inês A. T. A1 - Grigoryev, Dmitry A1 - Lewis, David M. G. A1 - Holford, Dawn L. A1 - Janssen, Steve M. J. A1 - Tatachari, Srinivasan A1 - Batres, Carlota A1 - Olofsson, Jonas K. A1 - Daches, Shimrit A1 - Belaus, Anabel A1 - Pfuhl, Gerit A1 - Corral-Frias, Nadia Sarai A1 - Sousa, Daniela A1 - Röer, Jan Philipp A1 - Isager, Peder Mortvedt A1 - Godbersen, Hendrik A1 - Walczak, Radoslaw B. A1 - Van Doren, Natalia A1 - Ren, Dongning A1 - Gill, Tripat A1 - Voracek, Martin A1 - DeBruine, Lisa M. A1 - Anne, Michele A1 - Očovaj, Sanja Batić A1 - Thomas, Andrew G. A1 - Arvanitis, Alexios A1 - Ostermann, Thomas A1 - Wolfe, Kelly A1 - Arinze, Nwadiogo Chisom A1 - Bundt, Carsten A1 - Lamm, Claus A1 - Calin-Jageman, Robert J A1 - Davis, William E. A1 - Karekla, Maria A1 - Zorjan, Saša A1 - Jaremka, Lisa M. A1 - Uttley, Jim A1 - Hricova, Monika A1 - Koehn, Monica A A1 - Kiselnikova, Natalia A1 - Bai, Hui A1 - Krafnick, Anthony J. A1 - Balci, Busra Bahar A1 - Ballantyne, Tonia A1 - Lins, Samuel A1 - Vally, Zahir A1 - Esteban-Serna, Celia A1 - Schmidt, Kathleen A1 - Macapagal, Paulo Manuel L. A1 - Szwed, Paulina A1 - Zdybek, Przemysław Marcin A1 - Moreau, David A1 - Collins, W. Matthew A1 - Joy-Gaba, Jennifer A. A1 - Vilares, Iris A1 - Tran, Ulrich S. A1 - Boudesseul, Jordane A1 - Albayrak-Aydemir, Nihan A1 - Dixson, Barnaby James Wyld A1 - Perillo, Jennifer T A1 - Ferreira, Ana A1 - Westgate, Erin C. A1 - Aberson, Christopher L. A1 - Arinze, Azuka Ikechukwu A1 - Jaeger, Bastian A1 - Butt, Muhammad Mussaffa A1 - Silva, Jaime R. A1 - Storage, Daniel Shafik A1 - Janak, Allison P A1 - Jiménez-Leal, William A1 - Soto, Jose A. A1 - Sorokowska, Agnieszka A1 - McCarthy, Randy A1 - Tullett, Alexa M A1 - Frias-Armenta, Martha A1 - Ribeiro, Matheus Fernando Felix A1 - Hartanto, Andree A1 - Forbes, Paul A. G. A1 - Willis, Megan L. A1 - del Carmen Tejada R, María A1 - Torres, Adriana Julieth Olaya A1 - Stephen, Ian D A1 - Vaidis, David C. A1 - de la Rosa-Gómez, Anabel A1 - Yu, Karen A1 - Sutherland, Clare A. M. A1 - Manavalan, Mathi A1 - Behzadnia, Behzad A1 - Urban, Jan A1 - Baskin, Ernest A1 - McFall, Joseph P. A1 - Ogbonnaya, Chisom Esther A1 - Fu, Cynthia H. Y. A1 - Rahal, Rima-Maria A1 - Ndukaihe, Izuchukwu L. G. A1 - Hostler, Thomas J. A1 - Kappes, Heather Barry A1 - Sorokowski, Piotr A1 - Khosla, Meetu A1 - Lazarevic, Ljiljana B. A1 - Eudave, Luis A1 - Vilsmeier, Johannes K. A1 - Luis, Elkin O. A1 - Muda, Rafał A1 - Agadullina, Elena A1 - Cárcamo, Rodrigo A. A1 - Reeck, Crystal A1 - Anjum, Gulnaz A1 - Venegas, Mónica Camila Toro A1 - Misiak, Michal A1 - Ryan, Richard M. A1 - Nock, Nora L. A1 - Travaglino, Giovanni A. A1 - Mensink, Michael C. A1 - Feldman, Gilad A1 - Wichman, Aaron L. A1 - Chou, Weilun A1 - Ziano, Ignazio A1 - Seehuus, Martin A1 - Chopik, William J. A1 - Kung, Franki Y. H. A1 - Carpentier, Joelle A1 - Vaughn, Leigh Ann A1 - Du, Hongfei A1 - Xiao, Qinyu A1 - Lima, Tiago J. S. A1 - Noone, Chris A1 - Onie, Sandersan A1 - Verbruggen, Frederick A1 - Radtke, Theda A1 - Primbs, Maximilian A. T1 - The psychological science accelerator’s COVID-19 rapid-response dataset T2 - Scientific Data Y1 - 2023 U6 - https://doi.org/10.1038/s41597-022-01811-7 SN - 2052-4463 VL - 10 IS - 1 SP - 1 EP - 15 PB - Springer Science and Business Media LLC ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Rödiger, Stefan A1 - Wex, Thomas A1 - Ansorge, Hermann A1 - Zinke, Olaf A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines T2 - Gut Pathogens N2 - Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli. Y1 - 2013 U6 - https://doi.org/10.1186/1757-4749-5-31 SN - 1757-4749 VL - 5 IS - 31 ER -