TY - GEN A1 - Azam, Hafiz Muhammad Husnain A1 - Rößling, Rosa Ilse A1 - Geithe, Christiane A1 - Khan, Muhammad Moman A1 - Dinter, Franziska A1 - Hanack, Katja A1 - Prüß, Harald A1 - Husse, Britta A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - MicroRNA biomarkers as next-generation diagnostic tools for neurodegenerative diseases: a comprehensive review T2 - Frontiers in Molecular Neuroscience N2 - Neurodegenerative diseases (NDs) are characterized by abnormalities within neurons of the brain or spinal cord that gradually lose function, eventually leading to cell death. Upon examination of affected tissue, pathological changes reveal a loss of synapses, misfolded proteins, and activation of immune cells—all indicative of disease progression—before severe clinical symptoms become apparent. Early detection of NDs is crucial for potentially administering targeted medications that may delay disease advancement. Given their complex pathophysiological features and diverse clinical symptoms, there is a pressing need for sensitive and effective diagnostic methods for NDs. Biomarkers such as microRNAs (miRNAs) have been identified as potential tools for detecting these diseases. We explore the pivotal role of miRNAs in the context of NDs, focusing on Alzheimer’s disease, Parkinson’s disease, Multiple sclerosis, Huntington’s disease, and Amyotrophic Lateral Sclerosis. The review delves into the intricate relationship between aging and NDs, highlighting structural and functional alterations in the aging brain and their implications for disease development. It elucidates how miRNAs and RNA-binding proteins are implicated in the pathogenesis of NDs and underscores the importance of investigating their expression and function in aging. Significantly, miRNAs exert substantial influence on post-translational modifications (PTMs), impacting not just the nervous system but a wide array of tissues and cell types as well. Specific miRNAs have been found to target proteins involved in ubiquitination or de-ubiquitination processes, which play a significant role in regulating protein function and stability. We discuss the link between miRNA, PTM, and NDs. Additionally, the review discusses the significance of miRNAs as biomarkers for early disease detection, offering insights into diagnostic strategies. KW - neurodegenerative diseases KW - microRNA KW - biomarkers KW - nervous system KW - diagnostic tools KW - therapeutic tools KW - protein post-translational modifications KW - limitations Y1 - 2024 U6 - https://doi.org/10.3389/fnmol.2024.1386735 SN - 1662-5099 VL - 17 PB - Frontiers Media S.A. ER - TY - GEN A1 - Geithe, Christiane A1 - Zeng, Bo A1 - Schmidt, Carsten A1 - Dinter, Franziska A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Dame, Gregory A1 - Schierack, Peter A1 - Hanack, Katja A1 - Rödiger, Stefan T1 - A multiplex microchamber diffusion assay for the antibody-based detection of microRNAs on randomly ordered microbeads T2 - Biosensors and Bioelectronics: X Y1 - 2024 U6 - https://doi.org/10.1016/j.biosx.2024.100484 SN - 2590-1370 VL - 18 (2024) SP - 1 EP - 7 PB - Elsevier BV ER -