TY - GEN A1 - Preußer, Daniel A1 - Bröring, Udo A1 - Fischer, Thomas A1 - Juretzek, Thomas T1 - Effects of antibiotics ceftriaxone and levofloxacin on the growth of Calliphora vomitoria L. (Diptera: Calliphoridae) and effects on the determination of the post-mortem interval T2 - Journal of Forensic and Legal Medicine N2 - The determination of the post-mortem interval (PMI) is one of the main tasks of forensic entomology, where growth and stages of development of arthropods are used for PMI determination. It is well acknowledged that maggot development is significantly influenced by temperature. Attention has also been paid to the microbial populations of the cadaver, because toxic substances contained in the substrate can influence the microorganisms and affect arthropods growth and development. However, little is known about the influence of antibiotics taken during lifetime of a person on insect development after that persons death. The aim of this study was to test the hypothesis that the antibiotics ceftriaxone and levofloxacin cause inhibition of growth and delay of pupation of the blow fly Calliphora vomitoria, which would then lead to an incorrect determination of the post-mortem interval in forensic cases. It was found that maggot development was delayed by levofloxacin mixed in minced pork, where a mixture of both antibiotics increased this effect. The maggot growth in the samples with ceftriaxone was not delayed. Pupation was delayed in treatments with a mixture of both antibiotics. The mortality was reduced by separate or combined application of ceftriaxone and levofloxacin, which we attribute to a bactericidal effect of the antibiotics on maggot pathogens. Depending on the concentration of the antibiotics, an underestimation of the post-mortem interval between 24 and 48 h could be suspected. We conclude that antibiotics need to be considered if instar stages are to be used to determine the PMI and that some antibiotics may improve the breeding conditions of maggots. KW - Antibiotics KW - Blowflies KW - Entomotoxicology KW - Post-mortem interval Y1 - 2021 U6 - https://doi.org/10.1016/j.jflm.2021.102207 SN - 1878-7487 SN - 1752-928X VL - 81 IS - 5 ER - TY - GEN A1 - Boch, Steffen A1 - Allan, Eric A1 - Birkhofer, Klaus A1 - Bossdorf, Oliver A1 - Blüthgen, Nico A1 - Christ-Breulmann, Sabina A1 - Diekötter, Tim A1 - Dormann, Carsten F. A1 - Gossner, Martin M. A1 - Hallmann, Christine A1 - Hemp, Andreas A1 - Hölzel, Norbert A1 - Jung, Kirsten A1 - Klaus, Valentin H. A1 - Klein, Alexandra M. A1 - Kleinebecker, Till A1 - Lange, Markus A1 - Müller, Jörg A1 - Nacke, Heiko A1 - Prati, Daniel A1 - Renner, Swen C. A1 - Rothenwöhrer, Christoph A1 - Schall, Peter A1 - Schulze, Ernst-Detlef A1 - Socher, Stephanie A. A1 - Tscharntke, Teja A1 - Türke, Manfred A1 - Weiner, Christiane N. A1 - Weisser, Wolfgang W. A1 - Westphal, Catrin A1 - Wolters, Volkmar A1 - Wubet, Tesfaye A1 - Fischer, Markus T1 - Extensive und jährlich wechselnde Nutzungsintensität fördert den Artenreichtum im Grünland T2 - ANLIEGEN NATUR Y1 - 2016 UR - https://www.biom.uni-freiburg.de/mitarbeiter/dormann/boch2016anliegennatur.pdf SN - 1864-0729 VL - 38 IS - 1 SP - 86 EP - 95 ER - TY - GEN A1 - Berkmann, F. A1 - Steuer, Oliver A1 - Ganss, Fabian A1 - Prucnal, Slawomir A1 - Schwarz, Daniel A1 - Fischer, Inga Anita A1 - Schulze, Jörg T1 - Sharp MIR plasmonic modes in gratings made of heavily doped pulsed laser-melted Ge1-xSnx T2 - Optical Materials Express Y1 - 2023 U6 - https://doi.org/10.1364/OME.479637 SN - 2159-3930 VL - 13 IS - 3 SP - 752 EP - 763 ER - TY - GEN A1 - Preußer, Daniel A1 - Fischer, Thomas A1 - Juretzek, Thomas T1 - Effects of antibiotics ceftriaxone and levofloxacin on the growth of Lucilia sericata (Diptera: Calliphoridae) T2 - Medical and Veterinary Entomology N2 - Lucilia sericata is one of the most studied species in forensic entomology due to its widespread distribution, forensic importance as well as medical use. The growth and development stage of maggots is often used to determine the post-mortem interval in forensic cases. L. sericata can cause myiasis in humans who are not able to maintain personal hygiene due to age or medical condition and can therefore be used to determine the time period of neglect. The influence of the temperature on the maggot development has been examined in various studies. Different examinations on the effects of toxic substances on the maggot development and survival have been conducted in order to test the influence and resulting deviations. In this study, the effects of different therapeutical doses of the antibiotics ceftriaxone and levofloxacin were examined on L. sericata in order to determine and compare deviations in maggot development and survival. The used antibiotics did not significantly influence the maggot weight and length. A significant delay in the time of pupation has been determined in the treatments with high concentrations of levofloxacin. The mortality was significantly increased in all treatments with antibiotics compared to the control, whereby the survivability of all treatments remained over 80%. Because of the high doses needed to produce an effect, we concluded that an overestimation of the post-mortem interval is unlikely but higher antibiotic concentrations or possible interactions with other medications could increase the maggot development time. KW - antibiotics KW - blow flies KW - entomotoxicology KW - myiasis KW - post‐mortem interval Y1 - 2023 U6 - https://doi.org/10.1111/mve.12685 SN - 0269-283X SN - 1365-2915 VL - 37 IS - 4 SP - 805 EP - 815 ER - TY - GEN A1 - Preußer, Daniel A1 - Fischer, Thomas A1 - Juretzek, Thomas T1 - Effects of antibiotics ceftriaxone and levofloxacin on the growth of Protophormia terraenovae (Diptera: Calliphoridae) T2 - Forensic Science, Medicine and Pathology N2 - AbstractProtophormia terraenovae is a colonizer of decomposing bodies and is known to cause pre-mortem myiasis as the female flies lay eggs in uncleaned wounds. In this study the effects of different concentrations of antibiotics levofloxacin and ceftriaxone on maggot development, weight, length, and mortality were examined. The maggot length and weight were significantly increased by therapeutical doses of levofloxacin and ceftriaxone. The maggot development time was significantly decreased in every levofloxacin treatment compared to the control. The time to start pupation was significantly increased in the control compared to the antibiotic treatments. Levofloxacin significantly increased the survivability of the maggots. Every levofloxacin treatment significantly improved the rearing conditions for the maggots. Reaching the third instar was delayed by 24 h in the control compared to the Levo 3.57 treatment. The Pupation in the control was delayed by an average of 48 h compared to the Levo 3.57 treatment. The significantly reduced development time of the maggots in the antibiotic treatments might lead to an overestimation of the post-mortem interval and therefore an incorrect time of death determination. The improved rearing conditions may be an indication of the potential of a combined application of antibiotics and maggot therapy. KW - General Medicine KW - Pathology and Forensic Medicine Y1 - 2024 U6 - https://doi.org/10.1007/s12024-024-00804-9 SN - 1556-2891 SP - 1 EP - 13 ER -