TY - GEN A1 - Hundt, Martin A1 - Engel, Norman A1 - Schnitzlein, Michael G. A1 - Schnitzlein, Klaus T1 - Alkalischer Polyolaufschluss als Basis für eine Lignocellulose-Bioraffinerie T2 - Chemie Ingenieur Technik KW - Aufschluss KW - Bioraffinerie KW - Cellulose KW - Delignifizierung KW - Enzymatische Hydrolyse KW - Fraktionierung KW - Lignin KW - Lignocellulose Y1 - 2013 U6 - https://doi.org/10.1002/cite.201200116 SN - 0009-286X VL - 85 IS - 5 SP - 758 EP - 763 ER - TY - GEN A1 - Engel, Norman A1 - Hundt, Martin A1 - Schapals, Tino T1 - Increasing the lignin yield of the Alkaline Polyol Pulping process by treating black liquor with laccases of Myceliophthora thermophila T2 - Bioresource Technology N2 - The Alkaline Polyol Pulping process separates cellulose from lignocellulosic biomass by dissolving lignin to a great extent. Due to the pulping conditions the dissolved lignin depolymerises and only 75% can be precipitated. To increase this amount, a 24 h reaction of laccases of Myceliophthora thermophila with lignin dissolved in black liquor of the AlkaPolP process was investigated. The influence of pH, temperature, enzyme concentration and partial oxygen pressure was examined in a batch stirred tank reactor using a Box–Behnken factorial design. Due to the enzymatic reaction the lignin polymerises which results in an enhanced lignin precipitation. The addition of a mediator improves the polymerisation but decreases the amount of precipitable lignin. The influence of the parameters on precipitation yield and molecular mass can sufficiently be described with a second-order model and optimum conditions can be assessed. FT-IR spectra of the obtained lignins revealed that its typical phenolic structure is preserved. KW - Biorefinery KW - Lignocellulose KW - Laccase KW - Polymerisation KW - Lignin Y1 - 2016 UR - http://www.sciencedirect.com/science/article/pii/S0960852415016570 U6 - https://doi.org/10.1016/j.biortech.2015.12.027 SN - 0960-8524 IS - 203 SP - 96 EP - 102 ER -