TY - GEN A1 - Volkov, Ilan A1 - Seguro, Luciana A1 - Leion, Elaine P. A1 - Kovács, László A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Gilburd, Boris A1 - Doria, Andrea A1 - Tektonidou, Maria G. A1 - Agmon-Levin, Nancy T1 - Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00131-3 SN - 2038-3274 SN - 2038-0305 VL - 11 SP - 1 EP - 8 ER - TY - GEN A1 - Ali, Aamir A1 - Kolenda, Rafał A1 - Khan, Muhammad Moman A1 - Weinreich, Jörg A1 - Li, Ganwu A1 - Wieler, Lothar H. A1 - Tedin, Karsten A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines T2 - Applied and Environmental Microbiology Y1 - 2020 U6 - https://doi.org/10.1128/AEM.01068-20 SN - 1098-5336 VL - 86 IS - 20 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure T2 - Heliyon Y1 - 2020 U6 - https://doi.org/10.1016/j.heliyon.2020.e03270 SN - 2405-8440 VL - 6 IS - 1 ER - TY - GEN A1 - Schiebel, Juliane A1 - Noack, Jonas A1 - Rödiger, Stefan A1 - Kammel, Anne A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Weise, Matthias A1 - Weiss, Romano A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Elimport, Alexey A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Analysis of three-dimensional biofilms on different material surfaces T2 - Biomaterials Science Y1 - 2020 U6 - https://doi.org/10.1039/D0BM00455C SN - 2047-4849 VL - 8 IS - 12 SP - 3500 EP - 3510 ER - TY - GEN A1 - Liedtke, Victoria A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Weiss, Romano A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Schenk, Lysann T1 - LEDGF/p75 is required for an efficient DNA damage response T2 - International Journal of Molecular Sciences KW - LEDGF KW - CRISPR/Cas9 KW - DNA damage signaling KW - gH2AX KW - ubiquitination Y1 - 2021 U6 - https://doi.org/10.3390/ijms22115866 VL - 22 IS - 11 SP - 1 EP - 16 ER - TY - GEN A1 - Comabella, Manual A1 - Deutschmann, Claudia A1 - Midaglia, Luciana A1 - Schierack, Peter A1 - Martínez, Júlia A1 - Roggenbuck, Dirk A1 - Montalban, Xavier T1 - Chitinase 3-like 1 is not a target antigen in patients with multiple sclerosis T2 - Multiple Sclerosis Journal Y1 - 2021 U6 - https://doi.org/10.1177/1352458520980141 SN - 1477-0970 SN - 1352-4585 VL - 27 IS - 9 SP - 1455 EP - 1457 ER - TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics T2 - GeroScience Y1 - 2021 U6 - https://doi.org/10.1007/s11357-021-00443-w SN - 2509-2723 SN - 2509-2715 VL - 43 IS - 5 SP - 2249 EP - 2264 ER - TY - GEN A1 - Schmidt, Carsten A1 - Borcherding, Heike A1 - Thiele, Thomas A1 - Schedler, Uwe A1 - Werner, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Fluorescence-encoded poly (methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis T2 - Analytical biochemistry Y1 - 2021 U6 - https://doi.org/10.1016/journal.ppat.1010118 SN - 1096-0309 VL - Vol. 633 ER - TY - GEN A1 - Emmenegger, Marc A1 - Kumar, Sreedhar Saseendran A1 - Emmenegger, Vishalini A1 - Malinauskas, Tomas A1 - Büttner, Thomas A1 - Rose, Laura A1 - Schierack, Peter A1 - Sprinzl, Martin F. A1 - Sommer, Clemens J. A1 - Lackner, Karl J. A1 - Aguzzi, Adriano A1 - Roggenbuck, Dirk A1 - Frauenknecht, Katrin B. M. T1 - Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins T2 - PLoS pathogens Y1 - 2021 U6 - https://doi.org/10.1371/journal.ppat.1010118 SN - 1553-7374 VL - 17 IS - 12 ER - TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test T2 - Scientific reports Y1 - 2021 U6 - https://doi.org/10.1038/s41598-021-00827-0 SN - 2045-2322 VL - 11 IS - 1 ER - TY - GEN A1 - Bartlitz, Christin A1 - Kolenda, Rafał A1 - Chilimoniuk, Jarosław A1 - Grzymajlo, Krzysztof A1 - Rödiger, Stefan A1 - Bauerfeind, Rolf A1 - Ali, Aamir A1 - Tchesnokovag, Veronika A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glyoprotein 2 T2 - Applied abd Environmental Microbiology Y1 - 2022 U6 - https://doi.org/10.1128/aem.02279-21 SN - 1098-5536 VL - 88 IS - 5 ER - TY - GEN A1 - Lopens, Steffi A1 - Wunsch, Ewa A1 - Milkiewicz, Malgorzata A1 - Röber, Nadja A1 - Zarske, Grit A1 - Nasser, Abdullah A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Rödiger, Stefan A1 - Krawczyk, Marcin A1 - Roggenbuck, Dirk A1 - Milkiewicz, Piotr T1 - PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis T2 - Diagnostics N2 - A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease. KW - primary sclerosing cholangitis KW - antibodies against serine protease proteinase-3 KW - survival KW - health-related quality of life KW - liver biochemistry KW - disease severity Y1 - 2022 U6 - https://doi.org/10.3390/diagnostics12112682 SN - 2075-4418 VL - 12 IS - 11 ER - TY - GEN A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Sack, Ulrich A1 - Anderer, Ursula A1 - Conrad, Karsten T1 - Die HEp-2-Zelle als Target für multiparametrische Autoantikörperanalytik – Automatisierung und Standardisierung/The HEp-2 cell as target for multiparametric autoantibody analyses: automation and standardisation T2 - Journal of Laboratory Medicine N2 - Trotz Einführung von Multiplex-Assays ist die Bestimmung von Autoantikörpern (AAK) mittels indirekter Immunfluoreszenz (IIF) nach wie vor ein wesentlicher Bestandteil der Autoimmundiagnostik. Das Screening auf nichtorganspezifische AAK wird in der Routinediagnostik von autoimmunen rheumatischen Erkrankungen mittels indirekter Immunfluoreszenz an HEp-2-Zellen durchgeführt. Leider differieren die mit diesem Test erhaltenen Befunde z. T. recht erheblich auf Grund von objektiven (z. B. HEp-2-Zellfixierung) und subjektiven (z. B. Erfahrungswerte) Faktoren. Die Intra- und Interlaborvarianzen sind daher relativ hoch. Erste Ergebnisse von Evaluierungsstudien eines neu entwickelten Systems zur automatischen Bildanalyse (AKLIDES®) zeigen einen Weg in Richtung Standardisierung (bzw. Harmonisierung) des HEp-2-Zell-Assays auf, in dem zunächst subjektive Fehlinterpretationen vermieden werden können. Eine weitere Harmonisierung erfordert die Weiterentwicklung der Software-Algorithmen zur Mustererkennung sowie neue Kalibrationssysteme. KW - Autoantikörper KW - Automatisierung KW - HEp-2 KW - Immunfluoreszenz Y1 - 2011 U6 - https://doi.org/10.1515/JLM.2011.053 SN - 1439-0477 VL - 35 IS - 6 SP - 351 EP - 361 ER - TY - GEN A1 - Azam, Hafiz Muhammad Husnain A1 - Rößling, Rosa Ilse A1 - Geithe, Christiane A1 - Khan, Muhammad Moman A1 - Dinter, Franziska A1 - Hanack, Katja A1 - Prüß, Harald A1 - Husse, Britta A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - MicroRNA biomarkers as next-generation diagnostic tools for neurodegenerative diseases: a comprehensive review T2 - Frontiers in Molecular Neuroscience N2 - Neurodegenerative diseases (NDs) are characterized by abnormalities within neurons of the brain or spinal cord that gradually lose function, eventually leading to cell death. Upon examination of affected tissue, pathological changes reveal a loss of synapses, misfolded proteins, and activation of immune cells—all indicative of disease progression—before severe clinical symptoms become apparent. Early detection of NDs is crucial for potentially administering targeted medications that may delay disease advancement. Given their complex pathophysiological features and diverse clinical symptoms, there is a pressing need for sensitive and effective diagnostic methods for NDs. Biomarkers such as microRNAs (miRNAs) have been identified as potential tools for detecting these diseases. We explore the pivotal role of miRNAs in the context of NDs, focusing on Alzheimer’s disease, Parkinson’s disease, Multiple sclerosis, Huntington’s disease, and Amyotrophic Lateral Sclerosis. The review delves into the intricate relationship between aging and NDs, highlighting structural and functional alterations in the aging brain and their implications for disease development. It elucidates how miRNAs and RNA-binding proteins are implicated in the pathogenesis of NDs and underscores the importance of investigating their expression and function in aging. Significantly, miRNAs exert substantial influence on post-translational modifications (PTMs), impacting not just the nervous system but a wide array of tissues and cell types as well. Specific miRNAs have been found to target proteins involved in ubiquitination or de-ubiquitination processes, which play a significant role in regulating protein function and stability. We discuss the link between miRNA, PTM, and NDs. Additionally, the review discusses the significance of miRNAs as biomarkers for early disease detection, offering insights into diagnostic strategies. KW - neurodegenerative diseases KW - microRNA KW - biomarkers KW - nervous system KW - diagnostic tools KW - therapeutic tools KW - protein post-translational modifications KW - limitations Y1 - 2024 U6 - https://doi.org/10.3389/fnmol.2024.1386735 SN - 1662-5099 VL - 17 PB - Frontiers Media S.A. ER - TY - GEN A1 - Frost, Fabian A1 - Weiss, Stefan A1 - Hertel, Johannes A1 - Rühlemann, Malte A1 - Bang, Corinna A1 - Franke, Andre A1 - Nauck, Matthias A1 - Dörr, Marcus A1 - Völzke, Henry A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Völker, Uwe A1 - Homuth, Georg A1 - Aghdassi, Ali A. A1 - Sendler, Matthias A1 - Lerch, Markus M. A1 - Weiss, Frank U. T1 - Fecal glycoprotein 2 is a marker of gut microbiota dysbiosis and systemic inflammation T2 - Gut Pathogens N2 - Background autoantigenic glycoprotein 2 (GP2) is an important component of the innate immune system which originates from the exocrine pancreas as well as from the small intestines. The relationship of GP2 with the intestinal microbiome as well as the systemic implications of increased fecal GP2 levels are, however, still unclear. Therefore, fecal samples from 2,812 individuals of the Study of Health in Pomerania (SHIP) were collected to determine GP2 levels (enzyme-linked immunosorbent assay) and gut microbiota profiles (16 S rRNA gene sequencing). These data were correlated and associated with highly standardised and comprehensive phenotypic data of the study participants. Results Fecal GP2 levels were increased in individuals with higher body mass index and smokers, whereas lower levels were found in case of preserved exocrine pancreatic function, female sex or a healthier diet. Moreover, higher GP2 levels were associated with increased serum levels of high-sensitivity C-reactive protein, loss of gut microbial diversity and an increase of potentially detrimental bacteria (Streptococcus, Haemophilus, Clostridium XIVa, or Collinsella). At the same time, predicted microbial pathways for the biosynthesis of beneficial short-chain fatty acids or lactic acid were depleted in individuals with high fecal GP2. Of note, GP2 exhibited a stronger association to overall microbiome variation than calprotectin. Conclusion Fecal GP2 is a biomarker of gut microbiota dysbiosis and associated with increased systemic inflammation. The intestines may be more important as origin for GP2 than pancreatic acinar cells. Future studies need to investigate the potential clinical value in disease specific patient cohorts. Y1 - 2024 U6 - https://doi.org/10.1186/s13099-024-00657-1 SN - 1757-4749 VL - 16 IS - 1 SP - 1 EP - 11 PB - Springer Science and Business Media LLC ER - TY - GEN A1 - Lopens, Steffi A1 - Schierack, Peter A1 - Krause, Jenny A1 - Piaszczyński, Michał A1 - Król, Robert A1 - Staroń, Robert A1 - Krupa, Łukasz A1 - Gutkowski, Krzysztof A1 - Kruk, Beata A1 - Grąt, Michał A1 - Krawczyk, Marek A1 - Patkowski, Waldemar A1 - Glaser, Fabian A1 - Rödiger, Stefan A1 - Grossmann, Kai A1 - Pająk, Jacek A1 - Milkiewicz, Piotr A1 - Lammert, Frank A1 - Zieniewicz, Krzysztof A1 - Schramm, Christoph A1 - Roggenbuck, Dirk A1 - Krawczyk, Marcin T1 - Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis T2 - Clinica Chimica Acta Y1 - 2024 U6 - https://doi.org/10.1016/j.cca.2024.119841 SN - 0009-8981 VL - 562 SP - 1 EP - 8 PB - Elsevier BV ER - TY - GEN A1 - Geithe, Christiane A1 - Zeng, Bo A1 - Schmidt, Carsten A1 - Dinter, Franziska A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Dame, Gregory A1 - Schierack, Peter A1 - Hanack, Katja A1 - Rödiger, Stefan T1 - A multiplex microchamber diffusion assay for the antibody-based detection of microRNAs on randomly ordered microbeads T2 - Biosensors and Bioelectronics: X Y1 - 2024 U6 - https://doi.org/10.1016/j.biosx.2024.100484 SN - 2590-1370 VL - 18 (2024) SP - 1 EP - 7 PB - Elsevier BV ER -