TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kuhl, Christoph A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Li, Ganwu A1 - Weinreich, Jörg A1 - Berger, Enrico A1 - Nolan, Lisa K. A1 - Nicholson, Bryon A1 - Römer, Antje A1 - Frömmel, Ulrike A1 - Wieler, Lothar H. A1 - Schröder, Christian T1 - Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method T2 - PLoS One N2 - We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars. Y1 - 2013 U6 - https://doi.org/10.1371/journal.pone.0059242 VL - 8 IS - 4 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Rödiger, Stefan A1 - Wex, Thomas A1 - Ansorge, Hermann A1 - Zinke, Olaf A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines T2 - Gut Pathogens N2 - Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli. Y1 - 2013 U6 - https://doi.org/10.1186/1757-4749-5-31 SN - 1757-4749 VL - 5 IS - 31 ER - TY - GEN A1 - Großmann, Kai A1 - Roggenbuck, Dirk A1 - Schröder, Christian A1 - Conrad, Karsten A1 - Schierack, Peter A1 - Sack, Ulrich T1 - Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay T2 - Cytometry Part A Y1 - 2011 U6 - https://doi.org/10.1002/cyto.a.21009 SN - 1552-4922 SN - 1552-4930 VL - 79 IS - 2 SP - 118 EP - 125 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Frömmel, Ulrike A1 - Schmidt, Carsten A1 - Ruhland, Mirko A1 - Schimke, Ingolf A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schröder, Christian ED - Seitz, Harald ED - Berger, Ingo T1 - A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies T2 - Molecular diagnostics Y1 - 2013 SN - 978-3-642-37690-0 SN - 978-3-642-37691-7 SP - 35 EP - 74 PB - Springer CY - Berlin [u.a.] ER - TY - GEN A1 - Nalli, Cecilia A1 - Somma, Valentina A1 - Andreoli, Laura A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Mahler, Michael A1 - Roggenbuck, Dirk A1 - Tincani, Angela T1 - Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases T2 - Autoimmunity Highlights Y1 - 2018 U6 - https://doi.org/10.1007/s13317-018-0106-0 SN - 2038-3274 SN - 2038-0305 VL - 9 IS - 1 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Rödiger, Stefan A1 - Sauer, Lysann A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Weinreich, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Gerber, Ulrike A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation T2 - Frontiers in Microbiology Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01905 SN - 1664-302X VL - 9 IS - 1905 ER - TY - GEN A1 - Sowa, Mandy A1 - Kolenda, Rafał A1 - Baumgart, Daniel C. A1 - Pratschke, Johann A1 - Papp, Maria A1 - Tornai, Tamas A1 - Suchanski, Jaroslaw A1 - Bogdanos, Dimitrios Petrou A1 - Mytilinaiou, Maria G. A1 - Hammermann, Jutta A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Schramm, Christoph A1 - Franke, Andre A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype T2 - Frontiers in Immunology Y1 - 2018 U6 - https://doi.org/10.3389/fimmu.2018.01959 SN - 1664-3224 VL - 9 IS - 1959 ER - TY - GEN A1 - Maslyanskiy, Aleksey A1 - Lazareva, Natalya A1 - Olinek, Polina A1 - Schierack, Peter A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Bogdanos, Dimitrios Petrou A1 - Lapin, Sergey V. A1 - Roggenbuck, Dirk T1 - Anti-hnRNP B1 (RA33) Autoantibodies Are Associated with the Clinical Phenotype in Russian Patients with Rheumatoid Arthritis and Systemic Sclerosis T2 - Journal of Immunology Research Y1 - 2014 U6 - https://doi.org/10.1155/2014/516593 VL - 2014 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Preface to the Special Issue on PCR on chip and related technologies T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1246-1 VL - 181 IS - 13/14 SP - 1609 EP - 1610 ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Hiemann, Rico T1 - Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen T2 - Journal of Laboratory Medicine Y1 - 2014 U6 - https://doi.org/10.1515/labmed-2014-0041 VL - 38 IS - 6 SP - 309 EP - 317 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Somma, Valentina A1 - Schierack, Peter A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi T1 - Autoantibody profiling in APS T2 - Lupus Y1 - 2014 U6 - https://doi.org/10.1177/0961203314534305 SN - 0961-2033 SN - 1477-0962 VL - 23 IS - 12 SP - 1262 EP - 1264 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Borghi, Maria Orietta A1 - Somma, Valentina A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Hanack, Katja A1 - Grossi, Claudia A1 - Bodio, Caterina A1 - Macor, Paolo A1 - Landenberg, Philipp von A1 - Boccellato, Francesco A1 - Mahler, Michael A1 - Meroni, Pier Luigi T1 - Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers T2 - Arthritis Research & Therapy Y1 - 2016 U6 - https://doi.org/10.1186/s13075-016-1018-x SN - 1465-9913 SN - 1478-6362 VL - 18 IS - 111 ER - TY - GEN A1 - Sowa, Mandy A1 - Trezzi, Barbara A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Somma, Valentina A1 - Sinico, Renato Alberto A1 - Roggenbuck, Dirk A1 - Radice, Antonella T1 - Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis T2 - Medicine Y1 - 2016 U6 - https://doi.org/10.1097/MD.0000000000005225 SN - 0025-7974 VL - 95 IS - 44 SP - 5225 ER - TY - GEN A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Frömmel, Ulrike A1 - Weinreich, Jörg A1 - Roggenbuck, Dirk A1 - Guenther, Sebastian A1 - Schaufler, Katharina A1 - Schröder, Christian A1 - Schierack, Peter T1 - Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season T2 - Environmental Microbiology Y1 - 2015 U6 - https://doi.org/10.1111/1462-2920.12807 SN - 1462-2920 SN - 1462-2912 VL - 17 IS - 9 SP - 3352 EP - 3361 ER - TY - GEN A1 - Villalta, Danilo A1 - Mytilinaiou, Maria G. A1 - Elsner, Martina A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Somma, Valentina A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Bogdanos, Dimitrios Petrou T1 - Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases T2 - Clinica Chimica Acta Y1 - 2015 U6 - https://doi.org/10.1016/j.cca.2015.07.021 SN - 0009-8981 VL - 450 SP - 5 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip T2 - Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 275 EP - 280 PB - Pabst Science Publ. CY - Lengerich [u.a.] ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Heiserich, Lisa A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - S. 413 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Sauer, Lysann A1 - Reddig, Annika A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, Österreich Y1 - 2016 UR - https://f1000research.com/posters/5-350 ER - TY - GEN A1 - Sauer, Lysann A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.–8.04.2016, Oxford, United Kingdom Y1 - 2016 UR - https://f1000research.com/posters/5-706 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Dinter, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Comparison of Lab and Point of Care (POC) technologies – case study for CHI3L1, Potsdam Days on Bioanalysis Y1 - 2017 UR - https://www.researchgate.net/publication/321419307 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - GEN A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Roggenbuck, Dirk A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates T2 - Applied and Environmental Microbiology Y1 - 2017 U6 - https://doi.org/10.1128/AEM.01660-17 SN - 1098-5336 SN - 0099-2240 VL - 83 IS - 24 ER - TY - GEN A1 - Liebsch, Claudia A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Fruth, Angelika A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schedler, Uwe A1 - Juretzek, Thomas A1 - Schierack, Peter T1 - Solid-phase microbead array for multiplex O-serotyping of Escherichia coli T2 - Microchimica Acta Y1 - 2017 U6 - https://doi.org/10.1007/s00604-017-2088-4 SN - 0026-3672 SN - 1436-5073 VL - 184 IS - 5 SP - 1405 EP - 1415 ER -