TY - GEN A1 - Mauß, Fabian A1 - Nakov, Galin A1 - Wenzel, Paul A1 - Steiner, Rüdiger A1 - Krüger, Christian A1 - Zhang, Yongzeh A1 - Rawat, Rajesh A1 - Borg, Andreas A1 - Perlman, Cathleen A1 - Fröjd, Karin A1 - Lehtiniemi, Harry T1 - Soot Simulation under Diesel Engine Conditions Using a Flamelet Approach T2 - SAE International Journal of Engines Y1 - 2010 SN - 1946-3936 VL - 2 IS - 2 SP - 89 EP - 104 ER - TY - CHAP A1 - Ulrich, Rainer G. A1 - Schlegel, Matthias A1 - Mertens, Marc A1 - Groschup, Martin H. A1 - Schmidt-Chanasit, Jonas A1 - Plenge-Bönig, Anita A1 - Jacob, Jens A1 - Pelz, Hans-Joachim A1 - Freise, Jona A1 - Wenk, Matthias A1 - Thiel, Jörg A1 - Triebenbacher, Cornelia A1 - Schmolz, Eric A1 - Kurth, Andreas A1 - Krüger, Frank A1 - Rühe, Ferdinand A1 - Kiffner, Christian A1 - Ansorge, Hermann A1 - Gerwin, Werner A1 - Wegener, Wolfgang A1 - Müller, Jörg A1 - Bemmann, Margit A1 - Wolf, Ronny A1 - Otto, Lutz-Florian A1 - Oehme, Rainer A1 - Pfeffer, Martin A1 - Heckel, Gerald A1 - Schex, Susanne A1 - Essbauer, Sandra S. T1 - Netzwerk Nagetier-übertragene Pathogene: Monitoring von Hantavirus-Infektionen in Deutschland T2 - Wildhygiene, Wildtierkrankheiten, Parasiten, Epidemiologie Y1 - 2009 SN - 978-378-88131-2-3 SN - 1436-3895 SP - 229 EP - 250 PB - Ges. für Wildtier- und Jagdforschung CY - Halle/Saale ER - TY - CHAP A1 - Fischer, Ulrich A1 - Krautz, Hans Joachim A1 - Wenske, Michael A1 - Tannert, Daniel A1 - Krüger, Perco A1 - Ziems, Christian A1 - Stolten, Detlef A1 - Emonts, Bernd T1 - Hydrogen Hybrid Power Plant in Prenzlau, Brandenburg T2 - Hydrogen Science and Engineering - Materials, Processes, Systems and Technology, Bd. 2 Y1 - 2016 SN - 978-3-5273323-8-0 SP - 1033 EP - 1052 PB - Wiley-VCH CY - Weinheim ER - TY - GEN A1 - Schulz, Christian A1 - Krüger-Genge, Anne A1 - Lendlein, Andreas A1 - Küpper, Jan-Heiner A1 - Jung, Friedrich T1 - Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture T2 - International Journal of Molecular Science N2 - The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used. KW - human venous endothelial cells KW - adherent KW - non-adherent KW - viability KW - mediator release Y1 - 2021 UR - https://www.mdpi.com/1422-0067/22/3/1493 U6 - https://doi.org/10.3390/ijms22031493 VL - 22 IS - 3 ER - TY - GEN A1 - Krüger-Genge, Anne A1 - Jung, Friedrich A1 - Küpper, Jan-Heiner A1 - Lehmann, Christian A1 - Franke, Ralf-Peter T1 - Actin type and distribution in erythrocytes T2 - Journal of Cellular Biotechnology N2 - Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation. KW - Erythrocytes KW - echinocytes KW - submembraneous cytoskeleton KW - actin Y1 - 2018 UR - https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb179014 U6 - https://doi.org/10.3233/JCB-179014 SN - 2352-3697 SN - 2352-3689 VL - 3 IS - 2 SP - 81 EP - 83 ER - TY - GEN A1 - Krüger-Genge, Anne A1 - Köhler, Susanne A1 - Laube, Markus A1 - Haileka, Vanessa A1 - Lemm, Sandy A1 - Majchrzak, Karolina A1 - Kammerer, Sarah A1 - Schulz, Christian A1 - Storsberg, Joachim A1 - Pietzsch, Jens A1 - Küpper, Jan-Heiner A1 - Jung, Friedrich T1 - Anti-Cancer Prodrug Cyclophosphamide Exerts Thrombogenic Effects on Human Venous Endothelial Cells Independent of CYP450 Activation—Relevance to Thrombosis T2 - Cells N2 - Cancer patients are at a very high risk of serious thrombotic events, often fatal. The causes discussed include the detachment of thrombogenic particles from tumor cells or the adverse effects of chemotherapeutic agents. Cytostatic agents can either act directly on their targets or, in the case of a prodrug approach, require metabolization for their action. Cyclophosphamide (CPA) is a widely used cytostatic drug that requires prodrug activation by cytochrome P450 enzymes (CYP) in the liver. We hypothesize that CPA could induce thrombosis in one of the following ways: (1) damage to endothelial cells (EC) after intra-endothelial metabolization; or (2) direct damage to EC without prior metabolization. In order to investigate this hypothesis, endothelial cells (HUVEC) were treated with CPA in clinically relevant concentrations for up to 8 days. HUVECs were chosen as a model representing the first place of action after intravenous CPA administration. No expression of CYP2B6, CYP3A4, CYP2C9 and CYP2C19 was found in HUVEC, but a weak expression of CYP2C18 was observed. CPA treatment of HUVEC induced DNA damage and a reduced formation of an EC monolayer and caused an increased release of prostacyclin (PGI2) and thromboxane (TXA) associated with a shift of the PGI2/TXA balance to a prothrombotic state. In an in vivo scenario, such processes would promote the risk of thrombus formation. KW - cancer KW - cyclophosphamide KW - human umbilical vein endothelial cells KW - HUVEC KW - liver KW - cytochrome P450 enzymes (CYP) KW - thrombosis Y1 - 2023 UR - https://www.mdpi.com/2073-4409/12/15/1965 U6 - https://doi.org/10.3390/cells12151965 SN - 2073-4409 VL - 12 IS - 15 ER - TY - GEN A1 - Jung, Ernst Michael A1 - Moran, Valentina Ocañaa A1 - Engel, Martin A1 - Krüger-Genge, Anne A1 - Stroszczynski, Christian A1 - Jung, Friedrich T1 - Modified contrast-enhanced ultrasonography with the new high-resolution examination technique of high frame rate contrast-enhanced ultrasound (HiFR-CEUS) for characterization of liver lesions: First results T2 - Clinical Hemorheology and Microcirculation Y1 - 2022 U6 - https://doi.org/10.3233/CH-221449 SN - 1875-8622 SP - 1 EP - 16 ER - TY - GEN A1 - Schulz, Christian A1 - Krüger-Genge, Anne A1 - Jung, Friedrich A1 - Lendlein, Andreas T1 - Aptamer supported in vitro endothelialization of poly(ether imide) films T2 - Clinical Hemorheology and Microcirculation N2 - Implantation of synthetic small-diameter vascular bypass grafts is often associated with an increased risk of failure, due to thrombotic events or late intimal hyperplasia. As one of the causes an insufficient hemocompatibility of the artificial surface is discussed. Endothelialization of synthetic grafts is reported to be a promising strategy for creating a self-renewing and regulative anti-thrombotic graft surface. However, the establishment of a shear resistant cell monolayer is still challenging. In our study, cyto- and immuno-compatible poly(ether imide) (PEI) films were explored as potential biomaterial for cardiovascular applications. Recently, we reported that the initial adherence of primary human umbilical vein endothelial cells (HUVEC) was delayed on PEI-films and about 9 days were needed to establish a confluent and almost shear resistant HUVEC monolayer. To accelerate the initial adherence of HUVEC, the PEI-film surface was functionalized with an aptamer-cRGD peptide based endothelialization supporting system. With this functionalization the initial adherence as well as the shear resistance of HUVEC on PEI-films was considerable improved compared to the unmodified polymer surface. The in vitro results confirm the general applicability of aptamers for an efficient functionalization of substrate surfaces. KW - aptamer KW - endothelialization KW - physical functionalization KW - poly(ether imide) films KW - shear resistance Y1 - 2020 U6 - https://doi.org/10.3233/CH-190775 SN - 1875-8622 SN - 1386-0291 VL - 75 IS - 2 SP - 201 EP - 217 ER - TY - GEN A1 - Wenzel, Paul A1 - Gezgin, A. A1 - Steiner, Rüdiger A1 - Krüger, Christian A1 - Netzell, Karl A1 - Lehtiniemi, Harry A1 - Mauß, Fabian T1 - Modeling of the soot particle size distribution in diesel engines T2 - Conference proceedings, Conference on Thermo- and Fluid Dynamic Processes in Diesel Engines, September 12th - 15th 2006, Valencia, Spain Y1 - 2006 SN - 84-9705-982-4 SP - 397 EP - 410 PB - Univ. Politécnica CY - Valencia ER - TY - CHAP A1 - Ziems, Christian A1 - Tannert, Daniel A1 - Tillmann, Christine A1 - Krüger, Perco A1 - Krause, S. A1 - Krautz, Hans Joachim T1 - Untersuchungen zur fortschrittlichen alkalischen Druckelektrolyse am Wasserstoff-Forschungszentrum Cottbus Y1 - 2011 ER -