TY - GEN A1 - Otto, Pascal A1 - Abendroth, Christian ED - Bockreis, Anke ED - Faulstich, Martin ED - Flamme, Sabine ED - Kranert, Martin ED - Mocker, Mario ED - Nelles, Michael ED - Quicker, Peter ED - Rettenberger, Gerhard ED - Rotter, Vera Susanne T1 - Untersuchung der mikrobiellen Profile von 70 verschiedenen Biogasanlagen und Identifizierung signifikanter technischer und chemischer Einflussgrößen auf das Mikrobiom T2 - 12. Wissenschaftskongress Abfall- und Ressourcenwirtschaft am 9. und 10. März 2023, Technischen Universität Hamburg, Tagungsband N2 - Das Mikrobiom umfasst die Gesamtheit aller Mikroorganismen, die ein Makroorganismus (Mensch, Tiere, Boden oder Biogasanlage) besiedelt. Aufgrund der Komplexität und Individualität der interagierenden Parameter ist das Zusammenwirken des Mikrobioms nicht ausreichend erforscht. Diese Studie fokussiert auf die Beeinflussung des Mikrobioms von Biogasanlagen durch chemische, technische und mikrobielle Faktoren. KW - Biogas KW - Mikrobiome Y1 - 2023 SN - 978-3-99106-095-6 U6 - https://doi.org/10.15203/99106-095-6 SP - 297 EP - 301 PB - Innsbruck university press CY - Innsbruck ER - TY - GEN A1 - Oviedo-Ocaña, Edgar Ricardo A1 - Abendroth, Christian A1 - Dominguez Rivera, Isabel Cristina A1 - Sánchez Ferrer, Antoni A1 - Dornack, Christina T1 - Life cycle assessment of biowaste and green waste composting systems: A review of applications and implementation challenges T2 - Waste Management N2 - Composting is one of the most widely applied methods for recycling organic waste. This process has been proposed as one option that facilitates the reincorporation of materials into the production cycle. However, composting also generates environmental impacts. Life Cycle Assessment (LCA) is the most common approach to evaluate the environmental impacts of a process at different system stages. Nevertheless, applying LCA in composting facilities is challenging due to the extensive information required, the lack of standardization on the initial assumptions, the definition of system boundaries, and the high diversity of existing composting technologies. This paper systematically reviews LCA studies in biowaste and/or green waste composting. The study highlights the challenges that should be met in order to improving the application of LCA to evaluate the environmental impacts of this type or waste treatment strategy. The review protocol used identified 456 papers published between 2010 and 2022. After the screening, 56 papers were selected, read, and thoroughly analyzed. The results show that: i) about 68% of the studies aimed to compare composting with other solid waste management options; ii) there was a wide diversity among the impact categories considered, which predominantly included climate change and ozone depletion; iii) there was no consensus on the functional unit or the system boundaries; iv) the main gaseous emissions studied were ammonia, methane, and nitrogen oxide, which were generally determined by emission factors; v) the avoided environmental impacts associated with the end-product quality and its application as an organic amendment or soil improver were ignored. This work demonstrates the complexity of conducting credible and valid composting LCA studies and proposes seven recommendations for improving the application of this assessment methodology to analyze this waste management alternative. KW - Biowaste KW - Life Cycle Assessment KW - Composting KW - Environmental impact KW - Green waste Y1 - 2023 UR - https://www.sciencedirect.com/science/article/pii/S0956053X23005688 U6 - https://doi.org/10.1016/j.wasman.2023.09.004 SN - 1879-2456 SN - 0956-053X VL - 171 SP - 350 EP - 364 ER - TY - GEN A1 - Markowski, Jens A1 - Abendroth, Christian A1 - Lapushynska, Alina A1 - Lohse, Anja T1 - Experiences from the commissioning of a bioleaching plant for the recovery of gold from printed circuit boards T2 - Biomining´23 - 11th International Symposium on Biomining N2 - Production waste from the manufacture of PCB often contains gold-containing contact-strips, whereby the gold content is < 1 %. Recycling of these gold coatings with conventional melting processes is technically hardly, since the carrier materials often contain mechanical reinforcements and flame retardants in addition to thermosetting plastics. With mechanical recycling, gold losses of 50% are possible. Therefore, a bioleaching plant for the recovery of gold and copper as preliminary stage for the smelting process has been in operation on m&k´s site since May 2022. The bioleaching is realized with iron and sulfur oxidizing bacteria, e.g. Leptospirilum ferrooxidans. The apparatus consists of a 400 litres-leaching bioreactor and peripheral equipment (fermenter, cementation, filters). The plant can process up to 10 kg of gold-containing PCB waste per batch, while simultaneously regenerating a second batch of bioleaching fluid. The process can recover up to 450 mg goldtinsel and 25 g copperpowder per Kilogramm input. KW - Goldrecycling KW - Bioleaching KW - Biomining Y1 - 2023 UR - https://az659834.vo.msecnd.net/eventsairwesteuprod/production-mei-public/74ffa794ee6840e9879041ac3b34042f UR - https://mei.eventsair.com/biomining-23/programme PB - Mining Engineering CY - Falmouth (UK) ER - TY - GEN A1 - Otto, Pascal A1 - Witkabel, Philipp A1 - Barth, Maximilian A1 - Ammar, Aziz Ben A1 - Rocktaeschel, Benjamin A1 - Torrent, Daniel A1 - Latorre-Peréz, Adriel A1 - Krause, Maximilian A1 - Abendroth, Christian T1 - Adaptation of the anaerobic microbiome for in-situ power-to-CH4 processes through fuzzy logic control of H2 input T2 - Bioresource technology reports N2 - This study presents the first application of fuzzy logic control (FLC) for regulating in-situ biological methanation via H2 injection in a fixed-bed reactor. Given the role of CH4 as a storable and infrastructure-compatible energy carrier, the aim was to improve CH4 production by dynamically adapting the H2 supply to the metabolic capacity of the microbiome. This will pave the way for the biological utilisation and storage of renewable H2. Over a 226- day operational period, H2 input was gradually increased based on real-time headspace gas measurements. Coupling the gradual increase in H2 supply with real-time gas composition data allowed the microbiome to adapt dynamically to changing process conditions. This approach led to a 49 % increase in CH4 concentration, from 58.4 % to 87.0 %, and improved H2 conversion rates from 98.2 % to 99.0 %. Chemical analyses of COD, organic acids and nitrogen were performed, as well as taxonomic analyses using 16S rRNA sequencing, and multivariate methods were applied to confirm the adaptation of the microbiome to the FLC H2 injection. The adopted microbial community was dominated by strictly hydrogenotrophic methanogens, Methanoculleus and Methanobacterium, while increased abundances of Petrimonas, Rectinema, Syntrophomonas, and Geobacter indicate interspecies H2 transfer and syntrophic cooperation enhancing methanogenesis. These findings demonstrate that FLC-based H2 control enables dynamic adjustment of in-situ methanation, optimising the interaction between hydrogenogenic, fermentative, syntrophic acetate-oxidising, and hydrogenotrophic microorganisms. This work introduces an adaptive control strategy that supports stable and efficient bio-CH4 production and represents a significant advance in the field of power-to-gas technologies. KW - In-situ methanation KW - Biological hydrogen methanation KW - Power-to-Gas KW - Adaptively evolved microbiome KW - 16S rRNA sequencing KW - Fuzzy logic control KW - Multivariate analysis Y1 - 2025 U6 - https://doi.org/10.1016/j.biteb.2025.102194 SN - 2589-014X VL - 31 SP - 1 EP - 11 PB - Elsevier BV CY - Amsterdam ER - TY - GEN A1 - Hozad, Ahmad Shabir A1 - Abendroth, Christian T1 - Electro-composting : an emerging technology T2 - Fermentation N2 - This study focuses on electrical stimulation for composting. Using the PSALSAR method, a comprehensive systematic review analysis identified 22 relevant articles. The examined studies fall into four main systems: electric field-assisted aerobic composting (EAAC), electrolytic oxygen aerobic composting (EOAC), microbial fuel cells (MFCs), and thermoelectric generators (TEGs). Apart from the main systems highlighted above, bioelectrochemically assisted anaerobic composting (AnCBE, III) is discussed as an underexplored system with the potential to improve the efficiency of anaerobic degradation. Each system is described in terms of key materials, composter design, operating conditions, temperature evolution, compost maturity, microbial community, and environmental outcomes. EAAC and EOAC systems accelerate organic matter decomposition by improving oxygen distribution and microbial activity, whereas MFC and TEG systems have dual functioning due to the energy generated alongside waste degradation. These innovative systems not only significantly improve composting efficiency by speeding up organic matter breakdown and increasing oxygen supply but also support sustainable waste management by reducing greenhouse gas emissions and generating bioelectricity or heat. Together, these systems overcome the drawbacks of conventional composting systems and promote future environmental sustainability solutions. Y1 - 2025 U6 - https://doi.org/10.3390/fermentation11070401 SN - 2311-5637 VL - 11 IS - 7 SP - 1 EP - 23 PB - MDPI AG CY - Basel ER - TY - GEN A1 - Puchol‐Royo, Roser A1 - Pascual, Javier A1 - Ortega‐Legarreta, Asier A1 - Otto, Pascal A1 - Tideman, Jeroen A1 - de Vries, Sjoerd‐Jan A1 - Abendroth, Christian A1 - Tanner, Kristie A1 - Porcar, Manuel A1 - Latorre‐Perez, Adriel T1 - Metagenomic insights into the ecology, taxonomy and metabolic capabilities of ‘Candidatus Darwinibacteriales’ Ord. Nov. (formerly MBA03), a potential key player in anaerobic digestionstion T2 - Microbial biotechnology N2 - Biogas, a mix of CO2, CH4 and small proportions of other gases, is a biofuel obtained by anaerobic digestion (AD). Biogas production is often considered a black box process, as the role and dynamics of some of the microorganisms involved remain undisclosed. Previous metataxonomic studies in the frame of the MICRO4BIOGAS project (www. micro 4biog as. eu) revealed that MBA03, an uncharacterised and uncultured bacterial taxon belonging to phylum Bacillota, was very prevalent and abundant in industrial full-scale AD plants. Despite the efforts, this taxon has not yet been cultivated, which makes the analysis of its taxonomy, ecology and metabolism even more challenging. In the present work, 30 samples derived from anaerobic digesters were sequenced, allowing the reconstruction of 108 metagenome-assembled genomes (MAGs) potentially belonging to MBA03. According to phylogenetic analyses and genomic similarity indices, MBA03 was classified as a new bacterial order, proposed as ‘Candidatus Darwinibacteriales’ ord. nov., which includes ‘Candidatus Darwinibacter acetoxidans’ gen. nov., sp. nov. of ‘Candidatus Darwinibacteriaceae’ fam. nov., along with ‘Candidatus Wallacebacter cryptica’ gen. nov., sp. nov. of the ‘Candidatus Wallacebacteriaceae’ fam. nov. Ecotaxonomic studies determined that AD processes are the main ecological niche of ‘Candidatus Darwinibacteriales’. Moreover, metabolic predictions identified Darwinibacteraceae members as putative syntrophic acetate-oxidising bacteria (SAOB), as they encode for the reversed Wood–Ljungdahl (W–L) pathway coupled to the glycine cleavage system. This suggests that Darwinibacteraceae members could work in collaboration with hydrogenotrophic methanogenic archaea to produce methane in industrial biogas plants. Overall, our findings present ‘Candidatus Darwinibacteriales’ as a potential key player in anaerobic digestion and pave the way towards the complete characterisation of this newly described bacterial taxon, which has not yet been cultured. Y1 - 2025 U6 - https://doi.org/10.1111/1751-7915.70258 SN - 1751-7915 VL - 18 IS - 12 SP - 1 EP - 16 PB - Wiley CY - Hoboken, NJ ER - TY - GEN A1 - Jansen, Nils A1 - Kaufman, Daniel A1 - Hozad, Ahmad Shabir A1 - Alipoursarbani, Mozhdeh A1 - Kochlamazashvili, Rati A1 - Kunchulia, Ilia A1 - Besalatpour, Aliasghar A1 - Abendroth, Christian T1 - From Waste to Wealth : Georgiens Bio-Fabrik für landwirtschaftliche Reststoffe T2 - Müll und Abfall : Fachzeitschrift für Kreislauf- und Ressourcenwirtschaft N2 - Die Landwirtschaft spielt in Georgien trotz zunehmender Urbanisierung eine zentrale wirtschaftliche Rolle, insbesondere in ländlichen und gebirgigen Regionen, wobei 16 % der Erwerbstätigen rund 6,2 % des BIP erwirtschaften. Herausforderungen wie geringe Wettbewerbsfähigkeit, unzureichende Landverteilung, fehlende moderne Technologien, geringe Abfallverwertung und Umweltbelastungen durch unsachgemäße Entsorgung von landwirtschaftlichen Rückständen erfordern innovative Ansätze. Das Waste2Wealth- Projekt (W2W), gefördert vom BMUKN, zielt darauf ab, diese Rückstände systematisch zu nutzen und Wissen, Technologie und Praxis miteinander zu verbinden. Hierzu werden Potenzialanalysen für landwirtschaftliche Reststoffe durchgeführt, Verwertungswege wie Biogasproduktion und Biokohleherstellung geprüft, und praxisnahe Instrumente wie das W2W-Whitebook, digitale Lernplattformen („Digitales LERNHAUS“), Train-of-Trainer-Module und ein mobiler Demonstrationscontainer implementiert. Das Projekt fördert Kapazitätsaufbau, Stakeholder-Engagement und den Technologietransfer zwischen Deutschland und Georgien, um eine zirkuläre Bioökonomie zu etablieren, die ökologische Nachhaltigkeit, wirtschaftliche Entwicklung und die Nutzung lokaler Ressourcen verbindet. Despite increasing urbanisation, agriculture plays a central economic role in Georgia, especially in rural and mountainous regions, with 16 % of the working population generating around 6.2 % of GDP. Challenges such as low competitiveness, inadequate land distribution, a lack of modern technologies, low waste recycling rates and environmental pollution caused by the improper disposal of agricultural residues require innovative approaches. The Waste2Wealth (W2W) project, funded by the BMUKN, aims to systematically utilise these residues and combine knowledge, technology and practice. To this end, potential analyses for agricultural residues are being carried out, recycling methods such as biogas production and biochar production are being examined, and practical tools such as the W2W White Paper, digital learning platforms (‚Digitales LERNHAUS‘), train-of-trainer modules and a mobile demonstration container are being implemented. The project promotes capacity building, stakeholder engagement and technology transfer between Germany and Georgia in order to establish a circular bioeconomy that combines ecological sustainability, economic development and the use of local resources. KW - Reststoffe KW - Landwirtschaft Y1 - 2026 U6 - https://doi.org/10.37307/j.1863-9763.2026.01.06 SN - 1863-9763 VL - 58 IS - 1 SP - 19 EP - 24 PB - Erich Schmidt Verlag GmbH & Co. KG CY - Berlin ER - TY - GEN A1 - Alipoursarbani, Mozhdeh A1 - Tideman, Jeroen A1 - López, Mitzy A1 - Abendroth, Christian T1 - Bioaugmentation in anaerobic digesters : a systematic review T2 - Biotechnology for biofuels and bioproducts N2 - Bioaugmentation, the intentional introduction of specific microorganisms into anaerobic digestion (AD) systems, has shown promise in enhancing methane production and in mitigating stressful conditions, particularly in systems operating below optimal performance. This review presents a systematic literature review (SLR) of research on bioaugmentation in AD. This review identified and analysed studies meeting predefined eligibility criteria through a structured methodology involving research protocol, search, appraisal, synthesis, analysis, and reporting. A notable innovation of this review is its comprehensive critical comparison of different controls used in bioaugmentation studies, which has been inadequately addressed in previous literature. To facilitate the functional understanding, strains for bioaugmentation were grouped into the four phases of anaerobic digestion (hydrolysis, acidogenesis, acetogenesis and methanogenesis). A highly diverse set of microbes has been described for bioaugmentation, especially from the families Clostridiaceae, Pseudomonadaceae and Syntrophomonadaceae. Most works are related to hydrolysis. The few works that address acidogenesis are mostly related to dark fermentation. Several studies used methanogenic archaea as well as syntrophic acetate oxidising bacteria, despite the difficulties in culturing them. On the other hand, studies applying strains for acetogenesis were largely underrepresented. Especially works on syntrophic propionate and butyrate oxidation (SPO and SBO) were missing. KW - Bioaugmentation KW - Anaerobic digestion KW - Biogas plants KW - Microorganisms KW - Microbiomes KW - Defined cultures Y1 - 2026 U6 - https://doi.org/10.1186/s13068-026-02746-6 SN - 2731-3654 VL - 19 IS - 1 SP - 1 EP - 26 PB - Springer Science and Business Media LLC CY - London ER -