TY - GEN A1 - Otto, Pascal A1 - Abendroth, Christian A1 - Dornack, Christina T1 - Designer-Mikrobiome für den Biogassektor T2 - Müll und Abfall N2 - Das Institut für Abfall- und Kreislaufwirtschaft (IAK) der TU Dresden ist am von der Europäischen Union im Rahmen des H2020-Programms geförderten Projekts "Micro4Biogas" beteiligt. Ziel des Projektes ist es, zur Unterstützung und Modernisierung des Biogassektors in den EU-Ländern beizutragen. Im Mittelpunkt stehen die Untersuchung der anaeroben Vergärung und des Mikrobioms, welche trotz jahrzehntelanger Forschung nicht ausreichend entschlüsselt sind. Obwohl das Wissen über die beteiligten Mikroorganismen zunimmt, wird das Mikrobiom nach wie vor als "Black Box" betrachtet. Im Rahmen von Micro4Biogas erfolgt die Charakterisierung natürlicher mikrobieller Gemeinschaften sowie die Erforschung neuer synthetischer mikrobieller Gemeinschaften. Darauf aufbauend erfolgt die Entwicklung von Strategien zur gezielten Beeinflussung des Mikrobioms (Bioaugmentation), die dazu dienen, den Prozess robuster, schneller, produktiver und standardisierter zu gestalten. Das bestehende und generierte Fachwissen wird in einer frei verfügbaren Roadmap zusammengefasst. Die Roadmap soll zudem Leitlinien und Handlungsempfehlungen für Industrie und Politik zur nachhaltigen und optimierten Produktion von Biogas enthalten. N2 - The institute of Waste Management and Circular Economy (IAK) is involved in the project "Micro4Biogas". This project is funded by the European Union under the H2020 programme and aims to contribute to the strengthening and modernisation of the biogas sector in the European Union. The focus is on the study of anaerobic digestion and the microbiome, which have not been sufficiently understood despite decades of research. Although knowledge about the involved microorganisms is increasing, the microbiome is still considered a "black box". Within the framework of Micro4Biogas, the characterization of natural microbial communities as well as the exploration of new synthetic microbial communities takes place. Based on this, strategies will be developed to specifically influence the microbiome (bioaugmentation), which will enable the process to become more robust, faster, more productive and more standardised. The existing and generated expertise will be summarised in a freely accessible roadmap. This also includes recommendations on how industry and politics can act to produce biogas in a sustainable and optimised way. KW - Biogas Y1 - 2022 UR - https://muellundabfall.de/ce/designer-mikrobiome-fuer-den-biogassektor/detail.html U6 - https://doi.org/10.37307/j.1863-9763.2022.05.05 SN - 1863-9763 VL - 54 IS - 05 SP - 243 EP - 247 ER - TY - GEN A1 - Otto, Pascal A1 - Abendroth, Christian T1 - The European Biogas Landscape: One of the most comprehensive studies on the biogas sector N2 - At the BIOENERGY DOC2022 (5th doctoral colloquium bioenergy), results from the currently running EU project Micro4Biogas were presented (https://micro4biogas.eu/). The presented poster describes the sampling of more than 70 samples from a large number of biogas plants in Germany and the Netherlands. Samples were analysed chemically and taxonomically. A comprehensive dataset was developed for the technical description of the respective plants. In the next step, a holistic, multivariate analysis of the collected data sets is planned. KW - Anaerobic digestions KW - microbiomes Y1 - 2022 UR - https://www.dbfz.de/en/5th-doctoral-colloquium-bioenergy CY - Leipzig ER - TY - GEN A1 - Abendroth, Christian A1 - Dornack, Christina A1 - Nefigmann, Sven A1 - Otto, Pascal ED - Nelles, Michael T1 - Mikroskopische Helden der Biogasbranche T2 - Tagungsband zum 20. Dialog Abfallwirtschaft und zum 16. Rostocker Bioenergieforum N2 - Das Streben nach umweltfreundlicher Energie und Wertstoffen hat in den letzten Jahren, gestärkt durch Wirtschaft und Politik, den Drang nach Innovation erheblich angeregt. Dieser Entwicklungsdrang findet sich in vielen Bereichen wieder, unter anderem in der Bioökonomie und hier auch bei Biogasanlagen. Das Schlagwort „Mikrobiom“ ist im medizinischen Bereich bereits weit verbreitet und wird im populärwissenschaftlichen Bereich in zahlreichen Büchern diskutiert. Dies unterstreicht das große gesellschaftliche Interesse an diesem Thema. Neben diesen Erkenntnissen gewinnt das Mikrobiom im Bereich der Bioenergien zunehmend an Bedeutung. In den letzten Jahren sind in hochrangigen, internationalen Fachjournalen zahlreichen Artikel erschienen, welche aufzeigen, dass Biogasanlagen wahre Superhelden unter den Mikroorganismen beherbergen. Die hohe Diversität wurde bereits in zahlreichen Publikationen diskutiert. Trotz dessen wird das Mikrobiom als „Blackbox“ betrachtet. Wissenschaftler weltweit versuchen diese Blackbox aufzulösen und hierbei traten einige Überraschungen auf. Dieses neue Wissen könnte den Grundstein für neue Konzepte der Prozesssteuerung legen. Ziel der vorliegenden Arbeit ist in diesem Zusammenhang eine Kurzübersicht über mikrobielle Innovation im Zusammenhang mit anaeroben Mikrobiomen. KW - Biogas, Mikrobiome Y1 - 2022 UR - https://bioenergieforum.auf.uni-rostock.de SN - 978-3-86009-535-5 U6 - https://doi.org/10.18453/rosdok_id00003615 SP - 233 EP - 241 CY - Rostock ER - TY - GEN A1 - Abendroth, Christian T1 - Zirkuläre Mikrobiome für Recycling- und Rückgewinnungsanwendungen T2 - Werkstoffe in der Fertigung N2 - Der Artikel stellt das Fachgebiet der Kreislaufwirtschaft an der BTU vor. Forschungsthemen des Fachgebietes werden kurz erläutert. Diese umfassen Biolaugung zur Behandlung von Leiterplatten, anaerobe Fermentation als Multiproduktplattform, Power2Gas Prozesse sowie das Verständnis mikrobieller Gemeinschaften mit Bedeutung für die Kreislaufwirtschaft. KW - Biogas KW - Mikrobiome KW - Power2Gas KW - Biolaugung Y1 - 2024 UR - https://werkstoffzeitschrift.de/werkstoffzeitschrift/ SN - 0939-2629 IS - 1 SP - 27 EP - 28 ER - TY - GEN A1 - Jansen, Nils A1 - Abendroth, Christian ED - Bockreis, Anke ED - Faulstich, Martin ED - Flamme, Sabine ED - Kranert, Martin ED - Mocker, Mario ED - Nelles, Michael ED - Quicker, Peter ED - Rettenberger, Gerhard ED - Rotter, Vera Susanne T1 - Unveiling the Biochar Potential: Lowering Greenhouse Gas Emissions across the Biogas Supply Chain T2 - Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universität Wien, Tagungsband N2 - The carbon-rich material known as biochar is the product of pyrolysis – a thermochemical conversion of biomass in the absence of oxygen. Given its characteristics, including long-term stability and sorption capacity, biochar has received increasing attention as a promising tool for addressing climate change and enhancing the production of biogas. This work combines both functions by examining, through different application scenarios, the potential role of biochar in mitigating greenhouse gas (GHG) emissions across the biogas supply chain. The wood-derived and high pyrolyzed biochar CarboFerm® was used as an example biochar for application in four different scenarios: (1) Application to soil without fertilizer, (2) application to soil with fertilizer, (3) application to biogas fermenter, (4) application to digestate treatment. The GHG mitigation was calculated by applying the methodology of the Renewable Energy Directive II (RED II). The scenario comparison resulted in GHG mitigation effectiveness of 0.03, 0.01, 0.06, and 0.03 g CO2eq / MJ biogas / t biochar, respectively. Thus, this study recommends using biochar in biogas fermenters and subsequently applying the biochar-enriched digestate to agricultural soil to reduce GHG emissions from biogas production. Furthermore, it is suggested that the mitigation potential of biochar may not be fully recognized in the RED, potentially resulting in an underestimation of its effectiveness. Y1 - 2024 SN - 978-3-99106-120-5 U6 - https://doi.org/10.15203/99106-120-5 SP - 279 EP - 286 PB - Innsbruck university press CY - Innsbruck ER - TY - GEN A1 - Alipoursarbani, Mozhdeh A1 - Abendroth, Christian ED - Bockreis, Anke ED - Faulstich, Martin ED - Flamme, Sabine ED - Kranert, Martin ED - Mocker, Mario ED - Nelles, Michael ED - Quicker, Peter ED - Rettenberger, Gerhard ED - Rotter, Vera Susanne T1 - Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge – A Micro Review T2 - Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universität Wien N2 - The authors of the present work summarize their recently published article “Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge” from Otto et al. (2023). This article presents the outcomes of a municipal-scale demonstrator plant implementing a recently patented process for improved sludge degradation within a municipal sewage treatment system. The study focused on a 1500 m³ sewage sludge digester, where an intermediate aerobic sewage sludge reactivation stage was introduced. This reactivation resulted in a 55% increase in biogas production and a 25% reduction in residual solids. For the residual NH4 in the liquid phase of the sewage sludge, the process achieved NH4-N removal rates exceeding 90%. To gain insights into the microbial community dynamics, 16S rRNA gene amplicon high-throughput sequencing was employed on the reactivated digestate. The analysis revealed a reduced population of methane-forming archaea compared to the primary digester, along with the detection of multiple ammonium-oxidizing bacteria. Notably, genera within the Chitinophagaceae family, comprising up to 18.8% of DNA sequences, were prevalent, alongside a small presence of Candidatus nitrosoglobus (<0.3%). The study highlights an economically viable approach for efficient nitrogen removal from sewage sludge while simultaneously increasing biogas yields and reducing potential pathogens in the residual material. KW - Biogas KW - Mikrobiome KW - Sewage Sludge Y1 - 2024 SN - 978-3-99106-120-5 U6 - https://doi.org/10.15203/99106-120-5 SP - 177 EP - 180 PB - Innsbruck university press CY - Innsbruck ER - TY - GEN A1 - Witkabel, Philipp A1 - Abendroth, Christian T1 - A systematic literature review of microbial anammox consortia in UASB/ EGSB-reactors T2 - Chemosphere N2 - Anaerobic ammonium oxidation (anammox) poses an emerging research field as it can outstand previous processes of biological wastewater treatment in terms of efficiency and costs. Anammox bacteria have the ability to metabolise NH4+ and NO2- to produce N2 under anaerobic conditions. Despite numerous studies, there is a lack of research on the co-occurrence and interrelationship of the predominant microbes that inhabit anammox-related processes. This systematic literature review follows the PSALSAR approach to assess metagenomic data on anammox bacteria and functional microbes in upstream reactors. Essential information on the physiology, metabolic pathways and inhibitory effects of anammox bacteria are reviewed and functional bacteria such as ammonia-oxidising bacteria (AOB), nitrite-oxidising bacteria (NOB), ammonia-oxidising Archaea (AOA) and denitrifying bacteria are identified. Candidatus Kuenenia and Candidatus Brocadia were the most frequently sequenced genera in the observed literature. Pseudomonadota, Chloroflexota and Bacteroidota were prevalent regardless of crucial operational parameters and configurations that affect the microbial community. Interrelationship analysis revealed a positive association between the versatility of a phylum's metabolism and its presence in the observed wastewater treatment literature. Several groups, such as Calditrichota, Myxococcota and Deinococcota are highly underrepresented, a finding that should be investigated in more detail. No evidence was found to suggest that high anammox ratios are correlated with high nitrogen removal efficiencies, as some studies found high efficiency despite low anammox abundance (<1%). Y1 - 2024 U6 - https://doi.org/10.1016/j.chemosphere.2024.143630 SN - 0045-6535 VL - 367 PB - Elsevier BV ER - TY - GEN A1 - Otto, Pascal A1 - Puchol-Royo, Roser A1 - Ortega-Legarreta, Asier A1 - Tanner, Kristie A1 - Tideman, Jeroen A1 - De Vries, Sjoerd-Jan A1 - Pascual, Javier A1 - Porcar, Manuel A1 - Latorre-Pérez, Adriel A1 - Abendroth, Christian T1 - Multivariate comparison of taxonomic, chemical and operational data from 80 different full-scale anaerobic digester-related systems T2 - Biotechnology for Biofuels and Bioproducts N2 - Background: The holistic characterization of different microbiomes in anaerobic digestion (AD) systems can contribute to a better understanding of these systems and provide starting points for bioengineering. The present study investigates the microbiome of 80 European full-scale AD systems. Operational, chemical and taxonomic data were thoroughly collected, analysed and correlated to identify the main drivers of AD processes. Results: The present study describes chemical and operational parameters for a broad spectrum of different AD systems. With this data, Spearman correlation and differential abundance analyses were applied to narrow down the role of the individual microorganisms detected. The authors succeeded in further limiting the number of microorganisms in the core microbiome for a broad range of AD systems. Based on 16S rRNA gene amplicon sequencing, MBA03, Proteiniphilum, a member of the family Dethiobacteraceae, the genus Caldicoprobacter and the methanogen Methanosarcina were the most prevalent and abundant organisms identified in all digesters analysed. High ratios for Methanoculleus are often described for agricultural co-digesters. Therefore, it is remarkable that Methanosarcina was surprisingly high in several digesters reaching ratios up to 47.2%. The various statistical analyses revealed that the microorganisms grouped according to different patterns. A purely taxonomic correlation enabled a distinction between an acetoclastic cluster and a hydrogenotrophic one. However, in the multivariate analysis with chemical parameters, the main clusters corresponded to hydrolytic and acidogenic microorganisms, with SAOB bacteria being particularly important in the second group. Including operational parameters resulted in digester-type specific grouping of microbes. Those with separate acidification stood out among the many reactor types due to their unexpected behaviour. Despite maximizing the organic loading rate in the hydrolytic pretreatments, these stages turned into extremely robust methane production units. Conclusions: From 80 different AD systems, one of the most holistic data sets is provided. A very distinct formation of microbial clusters was discovered, depending on whether taxonomic, chemical or operational parameters were combined. The microorganisms in the individual clusters were strongly dependent on the respective reference parameters. KW - anaerobic digestion, 16S rRNA gene amplicon sequencing, biogas plants Y1 - 2024 U6 - https://doi.org/10.1186/s13068-024-02525-1 VL - 17 SP - 1 EP - 17 ER - TY - GEN A1 - Otto, Pascal A1 - Abendroth, Christian T1 - Neue Bakterienordnung entdeckt T2 - Biogas Journal N2 - Wissenschaftler*innen des europäischen Forschungsprojektes Micro4Biogas haben eine neue taxonomische Ordnung von Bakterien entdeckt und klassifiziert, die auf die Zersetzung von organischem Material spezialisiert sind und der Schlüssel zu einer optimierten Biogasproduktion sein könnten. Die von ihnen als Darwinibacteriales bezeichnete Ordnung ist eine der am häufigsten vorkommenden Bakterien-Ordnungen in Biogasanlagen, wurde aber bisher noch nie wissenschaftlich klassifiziert. KW - Biogas, Mikrobiome, Darwinibacteriales Y1 - 2024 UR - https://www.biogas.org/edcom/webfvb.nsf/id/de-biogas-journal UR - https://ezb.ur.de/?2848895-7 SN - 1619-8913 VL - 2024 IS - 2 SP - 96 EP - 99 ER - TY - GEN A1 - Vidal-Verdú, Àngela A1 - Torrent, Daniel A1 - Iglesias, Alba A1 - Latorre-Pérez, Adriel A1 - Abendroth, Christian A1 - Corbín-Agustí, Paola A1 - Peretó, Juli A1 - Porcar, Manuel T1 - The highly differentiated gut of Pachnoda marginata hosts sequential microbiomes: microbial ecology and potential applications T2 - npj Biofilms and Microbiomes N2 - Insect gut microbiomes play a crucial role in the insect development and are shaped, among other factors, by the specialized insect diet habits as well as the morphological structure of the gut. Rose chafers (Pachnoda spp.; Coleoptera: Scarabaeidae) have a highly differentiated gut characterized by a pronounced hindgut dilation which resembles a miniaturized rumen. Specifically, the species Pachnoda marginata has not been previously studied in detail in terms of microbial ecology. Here, we show a fine scale study of the highly compartmentalized gut of P. marginata by using amplicon and metagenomic sequencing to shed light on the bacterial, archaeal and fungal communities thriving in each section of the gut. We found a microbial gradient along the gut from aerobic (foregut) to strictly anaerobic communities (hindgut). In addition, we have characterized interesting biological activities and metabolic pathways of gut microbial communities related to cellulose degradation, methane production and sulfate reduction. Taken together, our results reveal the highly diverse microbial community and the potential of P. marginata gut as a source of industrially relevant microbial diversity. Y1 - 2024 U6 - https://doi.org/10.1038/s41522-024-00531-7 SN - 2055-5008 VL - 10 IS - 1 PB - Springer Science and Business Media LLC ER -