TY - GEN A1 - Esperschütz, Jürgen A1 - Zimmermann, Claudia A1 - Dümig, Alexander A1 - Welzl, G. A1 - Buegger, Franz A1 - Elmer, Michael A1 - Munch, Jean Charles A1 - Schloter, Michael T1 - Dynamics of microbial communities during decomposition of litter from pioneering plants in initial soil ecosystems T2 - Biogeosciences Discussion Y1 - 2012 SN - 1810-6285 VL - 9 IS - 10 SP - 14981 EP - 15010 ER - TY - GEN A1 - Schulz, Stefanie A1 - Engel, Marion A1 - Fischer, Doreen A1 - Buegger, Franz A1 - Elmer, Michael A1 - Welzl, Gerhard A1 - Schloter, Michael T1 - Diversity pattern of nitrogen fixing microbes in nodules of Trifolium arvense (L.) at different initial stages of ecosystem development T2 - Biogeosciences KW - nitrogen fixing microbes Y1 - 2013 SN - 1726-4189 VL - 10 IS - 3 SP - 1183 EP - 1192 ER - TY - GEN A1 - Schulz, Stefanie A1 - Engel, Marion A1 - Fischer, Doreen A1 - Buegger, Franz A1 - Elmer, Michael A1 - Welzl, Gerhard A1 - Schloter, Michael T1 - Diversity patterns of nitrogen fixing microbes in nodules of Trifolium arvense (L.) at different initial stages of ecosystem development T2 - Biogeosciences Discussion Y1 - 2012 SN - 1810-6285 VL - 9 IS - 9 SP - 13135 EP - 13160 ER - TY - GEN A1 - Zyakun, Anatoly A1 - Nii-Annang, Seth A1 - Franke, Gabriele A1 - Fischer, Thomas A1 - Buegger, Franz A1 - Dilly, Oliver T1 - Microbial Actvity and 13C/12C Ratio as Evidence of N-Hexadecane and N-Hexadecanoic Acid Biodegradation in Agricultural and Forest Soils T2 - Geomicrobiology Journal N2 - The dynamics of microbial degradation of exogenous contaminants, n-hexadecane and its primary microbial oxidized metabolite, n-hexadecanoic (palmitic) acid, was studied for topsoils, under agricultural management and beech forest on the basis the changes in O2 uptake, CO2 evolution and its associated microbial and non-microbial carbon isotopic signature, the respiratory quotient (RQ) and the priming effect (PE) of substrates. Soil microbial communities in agricultural soil responded to the n-hexadecane addition more rapidly compared to those of forest soil, with lag-periods of about 23 ± 10 and 68 ± 13 hours, respectively. Insignificant difference in the lag-period duration was detected for agricultural (tlag = 30 ± 13 h) and forest (tlag = 30 ± 14 h) soils treated with n-hexadecanoic (palmitic) acid. These results demonstrate that the soil microbiota differed in metabolic activities for using n-hexadecane as a reductive hydrocarbon and n-hexadecanoic acid as a partly oxidized hydrocarbon. The corresponding δ13C of respired CO2 after the addition of the hydrocarbon contaminants to soils indicates a shift in microbial activity towards the consumption of exogenous substrates with a more complete degradation of n-hexadecane in the agricultural soil, for which some initial contents of hydrocarbons are inherent. It was reflected in the carbon isotope signature of microbial biomass. It is supposed that the observed deviation of RQ from theoretically calculated value under microbial substrate mineralization is determined by difference in the time (Δti) of registration of CO2 production and O2 consumption. Positive priming effect (PE) of n-hexadecane and negative PE of n-hexadecanoic (palmitic) acid were detected in agricultural and forest soils. It is suggested that positive PE of n-hexadecane is conditioned by the induction of microbial enzymes that perform hydroxylation/oxygenation of stable SOM compounds mineralized by soil microbiota to CO2. The microbial metabolism coupled with oxidative decarboxylation of n-hexadecanoic acid is considered as one of the most probable causes of the revealed negative PE value. Y1 - 2011 U6 - https://doi.org/10.1080/01490451.2010.489922 SN - 1521-0529 VL - 28 IS - 7 SP - 632 EP - 647 ER - TY - GEN A1 - Dilly, Oliver A1 - Nii-Annang, Seth A1 - Franke, Gabriele A1 - Fischer, Thomas A1 - Buegger, Franz A1 - Zyakun, Anatoly T1 - Resilience of microbial respiration, respiratory quotient and stable isotope characteristics to soil hydrocarbon addition T2 - Soil Biology and Biochemistry N2 - On the basis of CO2 evolution rate, O2 uptake rate, and 13C isotopic signature of respired CO2, the metabolic response to the addition of 13C labelled n-hexadecane and palmitic acid each with supplementary nitrogen was studied for two topsoils, one under continuous agricultural management and the other under beech forest. The CO2 evolution rate was immediately stimulated in the agricultural soil and the respiratory quotient (RQ) decreased from 0.8 to 0.4 mol CO2 evolution rate per mol O2 uptake rate, which was below the theoretically expected value of 0.65 and 0.70 for the degradation of n-hexadecane and palmitic acid, respectively. The microbial response was delayed in the forest soil, but developed better than in the agricultural soil throughout the subsequent 2–4 weeks. Consequently, the respiration rate returned earlier to the initial level for the beech forest soil and the δ13C of respired CO2 and RQ approached values before hydrocarbon addition. Based on the link among respiration rates, RQ and 13C–CO2 value, the added oil-analogue compounds induced a more rapid response in the agricultural soil and were degraded more completely in the forest soil. We concluded that the resilience, which we defined here as the capacity of the soil microbiota to buffer perturbance and to reorganise in response to change resulting in a more desirable system, was higher in our forest soil than for the agricultural soil. Y1 - 2011 U6 - https://doi.org/10.1016/j.soilbio.2010.09.026 SN - 0038-0717 VL - 43 IS - 9 SP - 1808 EP - 1811 ER - TY - GEN A1 - Esperschütz, Jürgen A1 - Zimmermann, Claudia A1 - Dümig, Alexander A1 - Welzl, G. A1 - Buegger, Franz A1 - Elmer, Michael A1 - Munch, Jean Charles A1 - Schloter, Michael T1 - Dynamics of microbial communities during decomposition of litter from pioneering plants in initial soil ecosystems T2 - Biogeosciences N2 - In initial ecosystems, concentrations of all macro- and micronutrients can be considered as extremely low. Plant litter therefore strongly influences the development of a degrader’s food web and is an important source for C and N input into soil in such ecosystems. In the present study, a 13C litter decomposition field experiment was performed for 30 weeks in initial soils from a post-mining area near the city of Cottbus (Germany). Two of this region’s dominant but contrasting pioneering plant species (Lotus corniculatus L. and Calamagrostis epigejos L.) were chosen to investigate the effects of litter quality on the litter decomposing microbial food web in initially nutrient-poor substrates. The results clearly indicate the importance of litter quality, as indicated by its N content, its bioavailability for the degradation process and the development of microbial communities in the detritusphere and soil. The degradation of the L. corniculatus litter, which had a low C / N ratio, was fast and showed pronounced changes in the microbial community structure 1–4 weeks after litter addition. The degradation of the C. epigejos litter material was slow and microbial community changes mainly occurred between 4 and 30 weeks after litter addition to the soil. However, for both litter materials clear indication of the importance of fungi for the degradation process was observed both in terms of fungal abundance and activity (13C incorporation activity). Y1 - 2013 U6 - https://doi.org/10.5194/bg-10-5115-2013 SN - 1726-4189 VL - 10 SP - 5115 EP - 5124 ER -