TY - GEN A1 - Boecker, Daniel A1 - Zybin, Alexander A1 - Niemax, Kay A1 - Grunwald, Christian A1 - Mirsky, Vladimir M. T1 - Noise reduction by multiple referencing in surface plasmon resonance imaging T2 - Review of Scientific Instruments N2 - The analytical performance of surface plasmon resonance imaging with charge coupled device detection can be improved significantly by splitting a macroscopic sensing surface into multiple microscopic neighboring sensing and referencing subareas. It is shown that such a multiple referencing reduces intensity fluctuations across the total sensing area and, therefore, improves the signal/noise (S/N)ratio proportional to the splitting factor. The approach is demonstrated by detection of biotin binding to a monolayer of streptavidin. An effective variation of the reflected intensity of about 10⁻⁴, which corresponds to the refraction index variation of 3 x 10⁻⁶, was detected with a S/N ratio about 10 without any temperature stabilization of the sensing area. Y1 - 2008 U6 - https://doi.org/10.1063/1.2888527 SN - 1527-2400 VL - 79 IS - 2 SP - 023110 ER - TY - GEN A1 - Zybin, Alexander A1 - Boecker, Daniel A1 - Mirsky, Vladimir M. A1 - Niemax, Kay T1 - Enhancement of the detection power of surface plasmon resonance measurements by optimization of the reflection angle T2 - Analytical Chemistry N2 - The detection limit of surface plasmon resonance (SPR) measurements has been improved by a factor of approximately 2-3.5 if the angle of incidence was near to the reflection minimum of the SPR resonance curve instead at the position of the steepest slope, the standard alignment in SPR imaging. The enhancement of the detection power, a result of signal-to-noise optimization, is demonstrated by applying a photodiode and a CCD camera for SPR detection. The experimental data are compared with data expected from theory. Y1 - 2007 U6 - https://doi.org/10.1021/ac070074u SN - 0003-2700 SN - 1520-6882 VL - 79 IS - 11 SP - 4233 EP - 4236 ER -