TY - GEN A1 - Fless, Friederike A1 - Graf, Bernhard A1 - Dally, Ortwin A1 - Franke, Ute A1 - Gerbich, Christine A1 - Lengyel, Dominik A1 - Knaut, Matthias A1 - Näser, Claudia A1 - Savoy, Bénédicte A1 - Steinmüller, Laura Katharina A1 - Steudtner, Katharina A1 - Taschner, Moritz A1 - Toulouse, Catherine A1 - Weber, Stefan T1 - Authenticity and Communication T2 - eTOPOI Journal for Ancient Studies N2 - Authenticity is not an absolute and constant quality inherent in an object or an experience; it is constructed in the process of research. Actors inscribe and attribute it to both material objects and subjective processes like communication and consumption. This article from the research group seeks on the one hand to reflect on the historical scope of action and action patterns among actors from various disciplines between the conflicting priorities of authentication and communication, and on the other to find ways to visualize and operationalize attribution processes through joint reflection. When we look at both history and the discussions fifty years after the Venice Charter, its idea to hand on historic monuments “in the full richness of their authenticity” has turned into an abundance of vibrant action and decision-making. KW - Visualisation Y1 - 2016 UR - http://journal.topoi.org/index.php/etopoi/article/view/263 SN - 2192-2608 VL - 6 SP - 479 EP - 524 ER - TY - GEN A1 - Lamby, Philipp A1 - Minkow, Alexander A1 - Handt, Stefan A1 - Falter, Johannes A1 - Schellenberg, Eva-Lotte A1 - Graf, Stefanie A1 - Hiebl, Bernhard A1 - Haerteis, Silke A1 - Gemeinhardt, Ole A1 - Krüger-Genge, Anne A1 - Klosterhalfen, Bernd A1 - Jung, Ernst Michael A1 - Franke, Ralf-Peter A1 - Momeni, Arash A1 - Prantl, Lukas A1 - Jung, Friedrich T1 - Histological and SEM assessment of blood stasis in kidney blood vessels after repeated intra-arterial application of radiographic contrast media T2 - Life N2 - Background: After application of iodinated contrast media (CM), a pronounced deterioration of the microcirculation in skin and myocardium was reported. Clinically, the repeated application of CM, especially, led to an increase of the renal resistance index (RRI). With respect to the transiency of the RRI increase, it is reasonable to assume that the deterioration of blood flow could be due to transient blood stasis caused by reversible morphologic cell alterations due to osmotic discrepancies between CM and human blood. Therefore, the hypothesis was investigated whether CM are able to induce in vivo such blood stasis and cell deformations in the renal vasculature of well-hydrated pigs. Methods: The in vivo study was performed as a prospective randomized examination to compare the effects of two different CM in 16 pigs (German Landrace). Pigs were randomized to receive either Iodixanol (n = 8), or Iopromide (n = 8). Each animal received 10 injections separated by 5-min intervals via the suprarenal aorta at a rate of 10 mL/s according to the usual procedure during a cardiac catheter examination. Finally, the kidneys were explanted and processed for histology (H & E staining and fibrin staining according to Weigert) as well as for scanning electron microscopy (SEM) with regards to morphologic correlates explaining the changes in the microcirculation. Results: In each of the predefined four categories of vascular diameters, blood stasis were found, but clearly more often after application of Iopromide than after application of Iodixanol (p < 0.001). In addition, Iopromide induced more blood stasis in all of the examined kidney regions compared to Iodixanol (p = 0.0001). There were no obstructive events in the middle cortex following the application of Iodixanol. Except for the region around a puncture channel of a placed-in catheter probe, no fibrin was detected in Weigert’s fibrin-stained samples, neither around the histologically assessed thrombi nor in vessels with blood stasis. Complementary SEM analyses revealed in a few cases only a slight generation of fibrin and thrombi and deformations, such as echinocyte and “box-like” deformations. Conclusions: According to previous in vitro studies, pathological erythrocyte deformations, such as echinocyte and box-like formation of erythrocytes, were observed also in vivo. In addition, blood stasis and/or thrombi could be detected in histological samples from explanted kidneys from young pigs after repeated in vivo administration of CM. In only a few cases, mural platelet aggregates within minimal fibrin meshes occurred only after the application of Iopromide. KW - acute kidney injury KW - nephrotoxicity KW - nephropathy KW - renal pathology KW - iodinated contrast media KW - electron microscopy KW - histopathology Y1 - 2020 UR - https://www.mdpi.com/2075-1729/10/9/167 U6 - https://doi.org/10.3390/life10090167 SN - 2075-1729 VL - 10 IS - 9 ER -