TY - GEN A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Brumma, Therese A1 - Büttner, Thomas A1 - Großmann, Kai A1 - Cusini, Marco A1 - Pregnolato, Francesca A1 - Borghi, Maria Orietta A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk A1 - Csernok, Elena T1 - Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis T2 - Arthritis Research & Therapy KW - ANCA-associated vasculitis KW - serology KW - pattern recognition algorithms KW - discrimination and classification of cytoplasmic ANCA (C-ANCA) and perinuclear ANCA (P-ANCA) KW - automated interpretation of ANCA IIF Y1 - 2012 UR - http://arthritis-research.com/content/14/6/R271 U6 - https://doi.org/10.1186/ar4119 SN - 1465-9913 SN - 1478-6354 VL - 14 ER - TY - GEN A1 - Nalli, Cecilia A1 - Somma, Valentina A1 - Andreoli, Laura A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Mahler, Michael A1 - Roggenbuck, Dirk A1 - Tincani, Angela T1 - Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases T2 - Autoimmunity Highlights Y1 - 2018 U6 - https://doi.org/10.1007/s13317-018-0106-0 SN - 2038-3274 SN - 2038-0305 VL - 9 IS - 1 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Borghi, Maria Orietta A1 - Somma, Valentina A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Hanack, Katja A1 - Grossi, Claudia A1 - Bodio, Caterina A1 - Macor, Paolo A1 - Landenberg, Philipp von A1 - Boccellato, Francesco A1 - Mahler, Michael A1 - Meroni, Pier Luigi T1 - Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers T2 - Arthritis Research & Therapy Y1 - 2016 U6 - https://doi.org/10.1186/s13075-016-1018-x SN - 1465-9913 SN - 1478-6362 VL - 18 IS - 111 ER - TY - GEN A1 - Thaler, Markus A. A1 - Bietenbeck, Andreas A1 - Steigerwald, Udo A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Lindhoff-Last, Edelgard A1 - Roggenbuck, Dirk A1 - Luppa, Peter B. T1 - Evaluation of the sensitivityficity of a novel line immunoassay for the detection of criteria and non-criteria antiphospholipid antibodies in comparison to establishedELISAs T2 - PLoS One Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0220033 SN - 1932-6203 VL - 14 IS - 7 ER - TY - GEN A1 - Emmenegger, Marc A1 - Kumar, Sreedhar Saseendran A1 - Emmenegger, Vishalini A1 - Malinauskas, Tomas A1 - Büttner, Thomas A1 - Rose, Laura A1 - Schierack, Peter A1 - Sprinzl, Martin F. A1 - Sommer, Clemens J. A1 - Lackner, Karl J. A1 - Aguzzi, Adriano A1 - Roggenbuck, Dirk A1 - Frauenknecht, Katrin B. M. T1 - Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins T2 - PLoS pathogens Y1 - 2021 U6 - https://doi.org/10.1371/journal.ppat.1010118 SN - 1553-7374 VL - 17 IS - 12 ER - TY - GEN A1 - Lyu, Yu-Xuan A1 - Fu, Qiang A1 - Wilczok, Dominika A1 - Ying, Kejun A1 - King, Aaron A1 - Antebi, Adam A1 - Vojta, Aleksandar A1 - Stolzing, Alexandra A1 - Moskalev, Alexey A1 - Georgievskaya, Anastasia A1 - Maier, Andrea B. A1 - Olsen, Andrea A1 - Groth, Anja A1 - Simon, Anna Katharina A1 - Brunet, Anne A1 - Jamil, Aisyah A1 - Kulaga, Anton A1 - Bhatti, Asif A1 - Yaden, Benjamin A1 - Pedersen, Bente Klarlund A1 - Schumacher, Björn A1 - Djordjevic, Boris A1 - Kennedy, Brian A1 - Chen, Chieh A1 - Huang, Christine Yuan A1 - Correll, Christoph U. A1 - Murphy, Coleen T. A1 - Ewald, Collin Y. A1 - Chen, Danica A1 - Valenzano, Dario Riccardo A1 - Sołdacki, Dariusz A1 - Erritzoe, David A1 - Meyer, David A1 - Sinclair, David A. A1 - Chini, Eduardo Nunes A1 - Teeling, Emma C. A1 - Morgen, Eric A1 - Verdin, Eric A1 - Vernet, Erik A1 - Pinilla, Estefano A1 - Fang, Evandro F. A1 - Bischof, Evelyne A1 - Mercken, Evi M. A1 - Finger, Fabian A1 - Kuipers, Folkert A1 - Pun, Frank W. A1 - Gyülveszi, Gabor A1 - Civiletto, Gabriele A1 - Zmudze, Garri A1 - Blander, Gil A1 - Pincus, Harold A. A1 - McClure, Joshua A1 - Kirkland, James L. A1 - Peyer, James A1 - Justice, Jamie N. A1 - Vijg, Jan A1 - Gruhn, Jennifer R. A1 - McLaughlin, Jerry A1 - Mannick, Joan A1 - Passos, João A1 - Baur, Joseph A. A1 - Betts-LaCroix, Joe A1 - Sedivy, John M. A1 - Speakman, John R. A1 - Shlain, Jordan A1 - Maltzahn, Julia von A1 - Andreasson, Katrin I. A1 - Moody, Kelsey A1 - Palikaras, Konstantinos A1 - Fortney, Kristen A1 - Niedernhofer, Laura J. A1 - Rasmussen, Lene Juel A1 - Veenhoff, Liesbeth M. A1 - Melton, Lisa A1 - Ferrucci, Luigi A1 - Quarta, Marco A1 - Koval, Maria A1 - Marinova, Maria A1 - Hamalainen, Mark A1 - Unfried, Maximilian A1 - Ringel, Michael S. A1 - Filipovic, Milos A1 - Topors, Mourad A1 - Mitin, Natalia A1 - Roy, Nawal A1 - Pintar, Nika A1 - Barzilai, Nir A1 - Binetti, Paolo A1 - Singh, Parminder A1 - Kohlhaas, Paul A1 - Robbins, Paul D. A1 - Rubin, Paul A1 - Fedichev, Peter O. A1 - Kamya, Petrina A1 - Muñoz-Canoves, Pura A1 - de Cabo, Rafael A1 - Faragher, Richard G. A. A1 - Konrad, Rob A1 - Ripa, Roberto A1 - Mansukhani, Robin A1 - Büttner, Sabrina A1 - Wickström, Sara A. A1 - Brunemeier, Sebastian A1 - Jakimov, Sergey A1 - Luo, Shan A1 - Rosenzweig-Lipson, Sharon A1 - Tsai, Shih-Yin A1 - Dimmeler, Stefanie A1 - Rando, Thomas A. A1 - Peterson, Tim R. A1 - Woods, Tina A1 - Wyss-Coray, Tony A1 - Finkel, Toren A1 - Strauss, Tzipora A1 - Gladyshev, Vadim N. A1 - Longo, Valter D. A1 - Dwaraka, Varun B. A1 - Gorbunova, Vera A1 - Acosta-Rodríguez, Victoria A. A1 - Sorrentino, Vincenzo A1 - Sebastiano, Vittorio A1 - Li, Wenbin A1 - Suh, Yousin A1 - Zhavoronkov, Alex A1 - Scheibye-Knudsen, Morten A1 - Bakula, Daniela T1 - Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity T2 - Aging Y1 - 2024 U6 - https://doi.org/10.18632/aging.206135 SN - 1945-4589 VL - 16 IS - 20 SP - 12955 EP - 12976 PB - Impact Journals, LLC ER - TY - GEN A1 - Roei, Tulchinsky A1 - Boris, Gilburd A1 - Yehuda, Shovman A1 - Milena, Tocut A1 - Eleanor, Zeruya A1 - Ariel, Binyaminov A1 - Tima, Davidson A1 - Büttner, Thomas A1 - Nasser, Abdullah A1 - Michel, Juliane A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Howard, Amital A1 - Roggenbuck, Dirk A1 - Yehuda, Shoenfeld A1 - Ora, Shovman T1 - CytoBead ANA 2 assay : a novel method for the detection of antinuclear antibodies T2 - Scientific reports N2 - Detection of anti-nuclear autoantibodies (ANA) is based on a two-step algorithm including indirect immunofluorescence (IIF) on HEp2 cells and subsequent reflex/confirmatory testing for specific autoantibodies. Simultaneous cell- and microbead-based autoantibody detection by IIF may be utilized for the evaluation of systemic autoimmune rheumatic diseases (SARDs). In the present study, we assessed the performance of CytoBead ANA 2 in the detection of ANA and ANA-specific autoantibodies, compared to ANA IIF and BioPlex™ 2200. We also tested the ability of CytoBead ANA DFS-70 to identify dense-fine speckled (DFS) pattern associated with anti-DFS70 antibodies in non-SARDs patients. Hundred-twelve routine sera samples were assessed by manual CytoBead ANA 2 for the presence of ANA and specific autoantibodies. In parallel, these samples were analyzed by HEp2 ANA IIF test and a subsequent multiplexed assay BioPlex™ 2200 ANA. Twenty-nine ANA-positive samples obtained from non-SARDs patients and exhibiting DFS pattern by ANA IIF were further tested by CytoBead ANA DFS-70. A substantial agreement was observed between classical ANA IIF and manual CytoBead ANA 2 for the detection of ANA (k = 0.74). Discordant results were mainly associated with the presence of anti-SSA/Ro antibodies detected by CytoBead ANA 2 in ANA IIF negative patients. A good to almost perfect agreement was found between CytoBead ANA 2 and BioPlex™ 2200 for detection of specific antibodies with kappa values ranging from 0.70 to 0.90. Twenty samples (68.9%) obtained from 29 ANA IIF positive without SARDs patients exhibited DFS pattern in CytoBead ANA DFS-70, which confirmed the presence of anti-DFS70 antibodies. The diagnostic performance of manual CytoBead ANA 2 for ANA screening and detection of ANA specific antibodies is comparable to the diagnostic performance of ANA IIF followed by BioPlex™ 2200. This novel one-step assay enables simultaneous ANA screening and confirmation and represents a promising alternative approach to the time-consuming and costly two-tier ANA analysis. KW - Anti-nuclear antibodies KW - Anti-DFS70 antibodies KW - Indirect immunofluorescence KW - Multiplexed assay KW - CytoBead technology Y1 - 2025 U6 - https://doi.org/10.1038/s41598-025-04583-3 SN - 2045-2322 VL - 15 IS - 1 SP - 1 EP - 11 PB - Nature Publishing Group UK CY - London ER -