TY - CHAP A1 - Nixdorf, Brigitte A1 - Becker, Katja A1 - Grüneberg, Björn A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Stickstoffumsätze in unterschiedlichen Gewässertypen Berlin/Brandenburgs, Teil 2: Erste Ergebnisse zu einträgen und Umsätzen T2 - Erweiterte Zusammenfassungen der Jahrestagung 2011 der Deutschen Gesellschaft für Limnologie (DGL) und der deutschen und österreichischen Sektion der Societas Internationalis Limnologiae (SIL), Weihenstephan, 12. - 16. Sept. 2011 Y1 - 2012 SN - 978-3-9813095-2-2 SP - 239 EP - 244 PB - Eigenverl. der DGL CY - Hardegsen ER - TY - CHAP A1 - Böllmann, Jörg A1 - Martienssen, Marion A1 - Grüneberg, Björn T1 - Vergleich der mikrobiellen Nitratreduktion in Sediment und Pelagial eines eutrophen Flusssees und eines mesotrophen geschichteten Sees T2 - Erweiterte Zusammenfassungen der Jahrestagungder Deutschen Gesellschaft für Limnologie (DGL), Potsdam - Berlin, 09. - 13. September 2013 KW - Nitratreduktion Y1 - 2014 SN - 978-3-9813095-5-3 SP - 35 EP - 39 PB - Hardegsen CY - Eigenverlag der DGL ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - Preliminary studies on the spread of the rust fungus Puccinia glechomatis in North America T2 - Joint Meeting of the American Phytopathological Society, the Mycological Society of America, and the Society of Nematologists, Salt Lake City, Utah, USA, August 25-29, 2001 Y1 - 2001 N1 - Phytopathology 21(2001)6 SP - S. 104 ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - The Life Cycle of Puccinia glechomatis, a microcyclic rust on Glechoma hederacea T2 - 2002 MSA Annual Meeting Corvallis, OREGON Y1 - 2002 UR - http://msafungi.org/wp-content/uploads/Inoculum/53%283%29.pdf N1 - Inoculum : newsletter of the Mycological Society of America 53(2002)3 SP - S. 21 ER - TY - GEN A1 - Rathsack, Kristina A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Comparative Study of Different Methods for Analyzing Denitrifying Bacteria in Fresh Water Ecosystems T2 - Journal of Water Resource and Protection N2 - Bacteria capable of denitrification play a significant role in the nitrogen cycle of freshwater ecosystems. By metabolizing nitrogen compounds they e.g. counteract the eutrophication of natural waters. To get detailed insights into the in situ turnover rates of nitrogen a reliable tool of quantification for active microorganisms is essential. In the present investigation, quantification capabilities of a molecular tool (Polymerase Chain Reaction—PCR) and a cultivation based tool (Most probable number—MPN) were investigated and compared. The total bacterial concentration yielded by the molecular PCR approach was up to 6-fold higher compared to the results of the MPN approach. However, the portion of culturable denitrifying bacteria compared to the number of specific gene copies (nirS) was much lower. Depending on the environmental conditions, the difference between the PCR and the MPN approach was up to three orders of magnitude. From lab scale experiments with a pure P. aeroginosa strain it can be concludes, that these differences are not the result of inappropriate culture conditions but rather reflect the portion of so called viable but not culturable bacteria (VBNC). Low nitrate concentrations as found in many fresh water ecosystems induced a significant increase in the portion of non culturable denitrifying bacteria. Referred to the investigation of dynamic populations, the number of metabolic active bacteria is represented by the MPN rather than by the PCR approach. Y1 - 2014 SN - 1945-3094 SN - 1945-3108 VL - 6 IS - 6 SP - 609 EP - 617 ER - TY - GEN A1 - Böllmann, Jörg A1 - Schöller, Markus T1 - Life cycle and life strategy features of Puccinia glechomatis (Uredinales) favorable for extending the natural range of distribution T2 - Mycoscience Y1 - 2006 U6 - https://doi.org/10.1007/s10267-006-0282-z SN - 1340-3540 VL - 47 IS - 3 SP - 152 EP - 158 ER - TY - CHAP A1 - Böllmann, Jörg T1 - Die Bedeutung von Anthropochorie und die Verbreitung von infiziertem Wirtsgewebe am Beispiel des Rostpilzes Puccinia glechomatis T2 - Treffpunkt biologische Vielfalt, aktuelle Forschung im Rahmen des Übereinkommens über die biologische Vielfalt vorgestellt auf einer wissenschaftlichen Expertentagung an der Internationalen Naturschutzakademie Insel Vilm vom 21. - 25. August 2006 Y1 - 2007 UR - http://www.bfn.de/fileadmin/MDB/documents/service/skript207.pdf SP - 107 EP - 112 PB - Bundesamt für Naturschutz CY - Bonn [u.a.] ER - TY - CHAP A1 - Scholler, Markus A1 - Böllmann, Jörg T1 - Puccinia glechomatis and P. lagenophorae in North America: invasion patterns, life cycle and life strategies T2 - Mycological Society of America (MSA) annual meeting, Hilo (Hawaii), 2006 Y1 - 2006 UR - http://msafungi.org/wp-content/uploads/Inoculum/56%284%29.pdf N1 - Inoculum : newsletter of the Mycological Society of America 56(2006)4 ER - TY - GEN A1 - Böllmann, Jörg A1 - Rathsack, Kristina A1 - Martienssen, Marion T1 - The precision of bacterial quantification techniques on different kinds of environmental samples and the effect of ultrasonic treatment T2 - Journal of Microbiological Methods N2 - The precision of cell number quantification in environmental samples depends on the complexity of the sample and on the applied technique. We compared fluorescence microscopy after filtration, quantification of gene copies and the cultivation based most probable number technique for their precision. We further analyzed the effect of increasing complexity of the sample material on the precision of the different methods by using pure cultures of Pseudomonas aeruginosa, fresh water samples and sediment slurries with and without ultrasonic treatment for analyses. Microscopy reached the highest precision, which was similar between pure cultures and water samples, but lower for sediment samples due to a higher percentage of cells in clusters and flocks. The PCR based quantification was most precise for pure cultures. Water and sediment samples were similar but less precise, which might be caused by the applied DNA extraction techniques. MPN measurements were equally precise for pure cultures and water samples. For sediment slurries the precision was slightly lower. The applied ultrasonic treatment of the slurries dispersed the cell clusters and flocks, increased the precision of microscopical and MPN measurements and also increased the number of potential colony forming units. However, the culturable cell number decreased by half. For MPN quantification of viable cells in samples with a high proportion of clustered cells we therefore recommend an optimization of ultrasonic treatment and a confirmation by microscopy and cultivation to reach highest possible dispersion of the cells with a minimum of inactivation. As a result of these observations we suggest a correction factor for MPN measurements to consider the effect of sonication on complex samples. The results are most likely applicable to other complex samples such as soil or biofilms. Y1 - 2016 U6 - https://doi.org/10.1016/j.mimet.2016.05.006 SN - 0167-7012 VL - 126 SP - 42 EP - 47 ER - TY - GEN A1 - Kuhn, Ramona A1 - Böllmann, Jörg A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples T2 - Journal of Microbiological Methods N2 - DNA extraction for molecular biological applications usually requires target optimized extraction procedures depending on the origin of the samples. For environmental samples, a range of different procedures has been developed. We compared the applicability and efficiency of ten selected DNA extraction methods published in recent literature using four different environmental samples namely: activated sludge from a domestic wastewater treatment plant, river sediment, anaerobic digestion sludge and nitrifying enrichment culture. We assessed the suitability of the extraction procedures based on both DNA yield and quality. DNA quantification was performed by both ultra violet (UV) spectrophotometry and fluorescence spectrophotometry after staining with PicoGreen. In our study, DNA yields based on UV measurement were overestimated in most cases while DNA yields from fluorescence measurements correlated well with the sample load on agarose gels of crude DNA. The quality of the DNA extracts was determined by gel electrophoresis of crude DNA and PCR products from 16S rDNA with the universal primer set 27f/1525r. It was observed that gel electrophoresis of crude DNA was not always suitable to evaluate DNA integrity and purity since interfering background substances (e.g. humic substances) were not visible. Therefore, we strongly recommend examining the DNA quality of both crude DNA and 16S rDNA PCR products by gel electrophoresis when a new extraction method is established. Summarizing, we found four out of ten extraction procedures being applicable to all tested samples without noticeable restrictions. The procedure G (according to the standard method 432_10401 of the Lower Saxony State Office for Consumer Protection and Food Safety) had the broadest application range over procedure J (published by Wilson, 2001). These were followed by procedures F (Singka et al., 2012) and A (Bourrain et al., 1999). All four extraction procedures delivered reliable and reproducible crude DNA and PCR products. From an economical point of view, all procedures tested during this study were cheaper compared to commercial DNA extraction kits. KW - DNA extraction KW - DNA quantification KW - DNA yield KW - Environmental samples KW - Gel electrophoresis KW - PicoGreen Y1 - 2017 U6 - https://doi.org/10.1016/j.mimet.2017.10.007 SN - 0167-7012 VL - 143 SP - 78 EP - 86 ER -