TY - CHAP A1 - Böhm, Birgit A1 - Böhm, Christian A1 - Huusmann, Peter A1 - Liesenfeld, Joachim A1 - Preising, Tobias A1 - Schindele, Stephan A1 - Streit, Anne von A1 - Strothe, Lena A1 - Terhorst, Alexandra ED - Schön, Susanne ED - Eismann, Christian ED - Wendt-Schwarzburg, Helke ED - Ansmann, Till T1 - Politik und Verwaltung konstruktiv stören T2 - Nachhaltige Landnutzung managen : Akteure beteiligen – Ideen entwickeln – Konflikte lösen Y1 - 2019 SN - 978-3-7639-6027-9 SP - 81 EP - 92 PB - wbv Media GmbH & Co KG CY - Bielefeld ER - TY - GEN A1 - Kanzler, Michael A1 - Böhm, Christian A1 - Quinkenstein, Ansgar A1 - Steinke, Christian A1 - Landgraf, Dirk T1 - Wuchsleistung der Robinie auf Lausitzer Rekultivierungsstandorten T2 - AFZ - der Wald Y1 - 2014 IS - 5 SP - 35 EP - 37 ER - TY - GEN A1 - Bärwolff, Manuela A1 - Vetter, Armin A1 - Böhm, Christian A1 - Hoffmann, Jörg A1 - Schmidt, Christian T1 - Projekt AgroForstEnergie ? Was bringen Streifen-Kup? T2 - energie pflanzen Y1 - 2011 SN - 1866-3036 IS - 2 SP - 10 EP - 12 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Rödiger, Stefan A1 - Wex, Thomas A1 - Ansorge, Hermann A1 - Zinke, Olaf A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines T2 - Gut Pathogens N2 - Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli. Y1 - 2013 U6 - https://doi.org/10.1186/1757-4749-5-31 SN - 1757-4749 VL - 5 IS - 31 ER - TY - GEN A1 - Rödiger, Stefan A1 - Ruhland, Mirko A1 - Schröder, Christian A1 - Großmann, Kai A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Schimke, Ingolf A1 - Schierack, Peter T1 - Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads T2 - Analytical Chemistry Y1 - 2011 U6 - https://doi.org/10.1021/ac103277s SN - 0003-2700 SN - 1520-6882 VL - 83 IS - 9 SP - 3379 EP - 3385 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Frömmel, Ulrike A1 - Schmidt, Carsten A1 - Ruhland, Mirko A1 - Schimke, Ingolf A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schröder, Christian ED - Seitz, Harald ED - Berger, Ingo T1 - A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies T2 - Molecular diagnostics Y1 - 2013 SN - 978-3-642-37690-0 SN - 978-3-642-37691-7 SP - 35 EP - 74 PB - Springer CY - Berlin [u.a.] ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip T2 - Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 275 EP - 280 PB - Pabst Science Publ. CY - Lengerich [u.a.] ER - TY - GEN A1 - Rödiger, Stefan A1 - Lehmann, Werner A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Schröder, Christian A1 - Dangla, Rémi A1 - Droniou, Magali A1 - Schierack, Peter T1 - The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 & 17. Leibniz Conference of Advanced Science, Freie Universität Berlin, 24.10.2013 Y1 - 2013 UR - https://leibniz-institut.de/Konferenzen/lsd2013/roediger_the_modi_operandi_of_the_videoscan_platform.pdf ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - CHAP A1 - Tsonkova, Penka A1 - Quinkenstein, Ansgar A1 - Böhm, Christian A1 - Freese, Dirk T1 - Assessment of Carbon Sequestration in German Alley Cropping Systems T2 - EGU General Assembly 2012, held 22-27 April, 2012 in Vienna N2 - Alley cropping systems (ACS) are agroforestry practices in which perennial trees or shrubs are grown in wide rows and arable crops are cultivated in the alleys between the tree rows. Recently, ACS which integrate stripes of short rotation coppices into conventional agricultural sites have gained interest in Germany. These systems can be used for simultaneous production of crops and woody biomass which enables farmers to diversify the provision of market goods. Adding trees into the agricultural landscape creates additional benefits for the farmer and society also known as ecosystem services. An ecosystem service provided by land use systems is carbon sequestration. The literature indicates that ACS are able to store more carbon compared to agriculture and their implementation may lead to greater benefits for the environment and society. Moreover, carbon sequestration in ACS could be included in carbon trading schemes and farmers rewarded additionally for the provision of this ecosystem service. However, methods are required which are easy to use and provide reliable information regarding change in carbon sequestration with change of the land use practice. In this context, our aim was to develop a methodology to assess carbon sequestration benefit provided by ACS in Germany. Therefore, the change of carbon in both soil and biomass had to be considered. To predict the change in soil carbon our methodology combined the 2006 IPCC Guidelines for National Greenhouse Gas Inventories and the soil organic carbon balance recommended by the Association of German Agricultural Investigation and Research Centers (VDLUFA). To reflect the change in biomass carbon average annual yields were adopted. The results showed that ACS established on agricultural sites can increase the carbon stored because in the new soil-plant system carbon content is higher compared to agriculture. ACS have been recommended as suitable land use systems for marginal sites, such as post-mining areas. In such areas soil carbon usually increases at a faster initial rate compared to agricultural land which means that these areas could provide high level of carbon sequestration service in the short term. The approach will be broadened to include assessment of other ecosystem services provided by ACS in Germany which would increase the possibility to adequately compensate farmers for the supply of environmental benefits. Y1 - 2012 UR - http://meetingorganizer.copernicus.org/EGU2012/EGU2012-12477.pdf N1 - EGU2012-12477 CY - European Geophysical Society ER - TY - CHAP A1 - Veste, Maik A1 - Böhm, Christian ED - Veste, Maik ED - Böhm, Christian T1 - Nachhaltige Holzproduktion in der Agrarlandschaft T2 - Agrarholz – Schnellwachsende Bäume in der Landwirtschaft : Biologie - Ökologie - Management Y1 - 2018 SN - 978-3-662-49930-6 SN - 978-3-662-49931-3 U6 - https://doi.org/10.1007/978-3-662-49931-3_1 SP - 1 EP - 16 PB - Springer Spektrum CY - Berlin [u.a.] ER - TY - CHAP A1 - Bärwolff, Manuela A1 - Böhm, Christian A1 - Schmidt, C. A1 - Schwarz, K.- U. A1 - Vetter, A. T1 - Combining short rotation coppice with annual crops - modern agroforestry systems for sustainable land use T2 - Proceedings of the 19th European Biomass Conference and Exhibition: From Research to Industry and Markets, 6-9 Juni 2011 in Berlin, Deutschland Y1 - 2011 SN - 978-88-89407-55-4 U6 - https://doi.org/10.5071/19thEUBCE2011-OB1.5 SP - 55 EP - 60 ER - TY - CHAP A1 - Böhm, Christian A1 - Quinkenstein, Ansgar A1 - Freese, Dirk A1 - Hüttl, Reinhard F. T1 - Energieholzproduktion in Agroforstsystemen als ein Beitrag zur Verbesserung des Bodenschutzes T2 - Bodenschutz in Europa, Ziele und Umsetzung, 6. Marktredwitzer Bodenschutztage, 06. - 08. Oktober 2010 Marktredwitz, Bayern Y1 - 2010 SP - 78 EP - 84 CY - Marktredwitz ER - TY - GEN A1 - Böhm, Christian A1 - Freese, Dirk A1 - Hüttl, Reinhard F. T1 - Energieholzproduktion in Agroforstsystemen - das Projekt AgroForstEnergie T2 - CEBra Newsletter Y1 - 2010 IS - 6 SP - 1 EP - 2 ER - TY - CHAP A1 - Kanzler, Michael A1 - Böhm, Christian A1 - Mirck, Jaconette A1 - Schmitt, Dieter A1 - Veste, Maik ED - Kage, Henning ED - Sieling, K. ED - Francke-Weltmann, L. T1 - Agroforstliche Nutzungsstrategien zur Sicherung der landwirtschaftlichen Produktion im Kontext steigender Urbanisierung und den Folgen des Klimawandels T2 - Anfoerungen an den Pflanzenbau in einer sich urbanisierenden Welt, 60. Jahrestagung der Gesellschaft für Pflanzenbauwissenschaften e.V., 26. bis 28. September 2017, Witzenhausen N2 - Derzeit lebt mehr als die Hälfte der Weltbevölkerung in Städten, Tendenz weiter steigend. Die Sicherstellung der Versorgung dieser Menschen mit Nahrungsmitteln wird künftig den Druck auf die Landwirtschaft in ländlichen Gebieten weiter erhöhen. Die Nahrungsmittelproduktion wird dabei stärker als zuvor durch das Umweltbewusstsein der Verbraucher einerseits und andererseits durch die Verminderung landwirtschaftlicher Produktionsfläche zugunsten der Stadtentwicklung beeinflusst werden (Vira et al. 2015). Die Folgen des Klimawandels (u.a. reduzierte Wasserverfügbarkeit, höheren Verdunstung, Zunahme von Witterungsextremen) werden zu Ertragsschwankungen führen (EEA 2017) und so die Landwirtschaft vor neue Herausforderungen stellen. Dies gilt vor allem für ertragsschwächere Standorte mit einem hohen Risiko für klimawandelbedingte Witterungseinflüsse. Vor diesem Hintergrund kann die agroforstliche Landnutzung, durch eine höhere Adaptionsfähigkeit gegenüber der Klimavariabilität (Kanzler et al. 2016) und der Möglichkeit zur ökologischen Aufwertung von Ackerflächen, aktiv zu einer stabilen, leistungsfähigen Landwirtschaft beitragen. Der folgende Beitrag widmet sich Untersuchungsergebnissen aus der Vegetationsperiode 2017 zum Mikroklima auf einem agroforstlich genutzten Ackerstandort in der Lausitz, Brandenburg. Y1 - 2017 UR - https://www.gpw.uni-kiel.de/de/jahrestagung/tagungsbaende/tagungsband-2017 SP - 54 EP - 55 PB - Liddy Halm CY - Göttingen ER - TY - CHAP A1 - Böhm, Christian A1 - Bärwolff, Manuela A1 - Quinkenstein, Ansgar A1 - Freese, Dirk ED - Nelles, Michael T1 - Bereitstellung von holzartigen Bioenergieträgern durch Agroforstsysteme T2 - Tagungsband, 5. Rostocker Bioenergieforum, am 2. und 3. November 2011 an der Universität Rostock, Y1 - 2011 SN - 978-3-940364-20-3 SP - 57 EP - 68 PB - Univ. CY - Rostock ER - TY - CHAP A1 - Tsonkova, Penka A1 - Freese, Dirk A1 - Böhm, Christian A1 - Quinkenstein, Ansgar A1 - Schaller, Eberhard ED - Freese, Dirk ED - Pittruff, N. ED - Quinkenstein, Ansgar ED - Böhm, Christian T1 - Benefits provided by silvoarable agroforestry systems in the temperate region: a review T2 - Proceedings of the International Workshop on Agroforestry - Ecological Benefits of Agroforestry, 15-17 June 2011, Cottbus, Germany Y1 - 2011 UR - http://www-docs.tu-cottbus.de/multiland/public/FreeseEtAl2011_Proceedings_of_the_International_Workshop_on_Agroforestry.pdf SP - S. 29 ER -