TY - GEN A1 - Kotlarski, Sven A1 - Block, Alexander A1 - Böhm, Ursula A1 - Jacob, Daniela A1 - Keuler, Klaus A1 - Knoche, Richard A1 - Rechid, Diana A1 - Walter, Artur T1 - Regional Climate Model Simulations as Input for Hydrological Applications: Evaluation of Uncertainties T2 - Advances in Geoscience Y1 - 2005 U6 - https://doi.org/10.5194/adgeo-5-119-2005 IS - 5 SP - 119 EP - 125 ER - TY - RPRT A1 - Böhm, Uwe A1 - Kücken, Martin A1 - Ahrens, Wilfried A1 - Block, Alexander A1 - Hauffe, Detlef A1 - Keuler, Klaus A1 - Rockel, Burkhardt A1 - Will, Andreas T1 - CLM - The Climate Version of LM: Brief Description and Long-Term Applications Y1 - 2006 UR - http://www.cosmo-model.org/content/model/documentation/newsLetters/newsLetter06/cnl6_clm.pdf PB - COSMO Newsletter CY - Offenbach, Germany ER - TY - GEN A1 - Octorina, Pelita A1 - Böhm, Alexander A1 - Martin-Creuzburg, Dominik T1 - Morphological defences and defence–cost trade-offs in Daphnia in response to two co-occurring invertebrate predators T2 - Freshwater Biology KW - morphological defences KW - multi-predator environment Y1 - 2022 U6 - https://doi.org/10.1111/fwb.13888 SN - 1365-2427 VL - 67 IS - 5 SP - 883 EP - 892 ER - TY - GEN A1 - Hiemann, Rico A1 - Hilger, Nadja A1 - Michel, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Anderer, Ursula A1 - Weigert, Martin A1 - Sack, Ulrich T1 - Automatic Analysis of Immunofluorescence Patterns of HEp‐2 Cells T2 - Annals of the New York Academy of Sciences N2 - The standard screening test for the recognition of autoimmune diseases is the proof of autoantibodies in serum of patients by indirect immunofluorescence (IIF) based on HEp‐2 cells. Manual evaluation of this test is very subjective, slow, and there are no objective parameters as guidelines available. Interlaboratory tests showed occasionally large deviations in the test evaluation resulting in a high variance of results. The aim of this project is fast, objective, safe, and economical automatic analysis of HEp‐2 IIF patterns. Images of IIF patterns were completely and automatically captured using an inverse motorized fluorescence microscope. Thereby, device‐specific parameters were controlled automatically, too. For fast analysis of IIF patterns new algorithms of image processing were developed. Artifacts were recognized and excluded from analysis by the developed software. Analysis of more than 80,000 images clearly demonstrated full automatization and fast processing of IIF patterns. Additionally serum‐specific fluorescence could be easily distinguished from background. Even very weak but positive patterns can be recognized and used for diagnosis. A detailed separation into different basic patterns is possible. Objective, fast, and disease‐related economical analysis of HEp‐2 immunofluorescence patterns is feasible. The implemented software algorithms allowed a mathematical way of describing IIF patterns and can therefore be a useful tool for the needed standardization process. KW - Autoimmune diagnostics KW - HEp-2 cell KW - Immunofluorescence KW - Automatic analysis KW - Screening Y1 - 2007 U6 - https://doi.org/10.1196/annals.1398.042 SN - 0077-8923 VL - 1109 IS - 1 SP - 358 EP - 371 ER - TY - GEN A1 - Böhm, Uwe A1 - Kücken, Martin A1 - Hauffe, Detlef A1 - Gerstengarbe, F.-W. A1 - Werner, P. C. A1 - Flechsig, Michael A1 - Keuler, Klaus A1 - Block, Alexander A1 - Ahrens, Wilfried A1 - Nocke, Th. T1 - Reliability of regional climate model simulations of extremes and of long-term climate T2 - Natural Hazards and Earth System Sciences N2 - We present two case studies that demonstrate how a common evaluation methodology can be used to assess the reliability of regional climate model simulations from different fields of research. In Case I, we focused on the agricultural yield loss risk for maize in Northeastern Brazil during a drought linked to an El-Niño event. In Case II, the present-day regional climatic conditions in Europe for a 10-year period are simulated. To comprehensively evaluate the model results for both kinds of investigations, we developed a general methodology. On its basis, we elaborated and implemented modules to assess the quality of model results using both advanced visualization techniques and statistical algorithms. Besides univariate approaches for individual near-surface parameters, we used multivariate statistics to investigate multiple near-surface parameters of interest together. For the latter case, we defined generalized quality measures to quantify the model's accuracy. Furthermore, we elaborated a diagnosis tool applicable for atmospheric variables to assess the model's accuracy in representing the physical processes above the surface under various aspects. By means of this evaluation approach, it could be demonstrated in Case Study I that the accuracy of the applied regional climate model resides at the same level as that we found for another regional model and a global model. Excessive precipitation during the rainy season in coastal regions could be identified as a major contribution leading to this result. In Case Study II, we also identified the accuracy of the investigated mean characteristics for near-surface temperature and precipitation to be comparable to another regional model. In this case, an artificial modulation of the used initial and boundary data during preprocessing could be identified as the major source of error in the simulation. Altogether, the achieved results for the presented investigations indicate the potential of our methodology to be applied as a common test bed to different fields of research in regional climate modeling. Y1 - 2004 U6 - https://doi.org/10.5194/nhess-4-417-2004 VL - 4 IS - 3 SP - 417 EP - 431 ER - TY - GEN A1 - Böhm, Uwe A1 - Kücken, Martin A1 - Ahrens, Wilfried A1 - Block, Alexander A1 - Hauffe, Detlef A1 - Keuler, Klaus A1 - Rockel, Burkhardt A1 - Will, Andreas T1 - The Climate Version of LM: Brief Description and Long-Term Applications T2 - COSMO Newsletter Y1 - 2006 UR - http://www.cosmo-model.org/content/model/documentation/newsLetters/newsLetter06/newsLetter_06.pdf IS - 6 SP - 225 EP - 235 ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Szabo, M. A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin T2 - 5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016 Y1 - 2016 UR - https://www.asm.org/images/1903%20asm%20salmonella%20program%20web3.pdf SP - S. 125 PB - American Society for Microbiology CY - Washington, DC ER - TY - GEN A1 - Awan, Asad Bashir A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Sarwar, Yasra A1 - Schierack, Peter A1 - Ali, Aamir T1 - Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa T2 - EXCLI journal Y1 - 2019 SN - 1611-2156 VL - 18 SP - 79 EP - 90 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Rödiger, Stefan A1 - Lehmann, Werner A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Schröder, Christian A1 - Dangla, Rémi A1 - Droniou, Magali A1 - Schierack, Peter T1 - The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 & 17. Leibniz Conference of Advanced Science, Freie Universität Berlin, 24.10.2013 Y1 - 2013 UR - https://leibniz-institut.de/Konferenzen/lsd2013/roediger_the_modi_operandi_of_the_videoscan_platform.pdf ER -