TY - GEN A1 - Pecyna, Marek J. A1 - Ullrich, René A1 - Bittner, Britta A1 - Clemens, André A1 - Scheibner, Katrin A1 - Schubert, Roland A1 - Hofrichter, Martin T1 - Molecular characterization of aromatic peroxygenase from Agrocybe aegerita T2 - Applied Microbiology and Biotechnology N2 - Recently, a novel group of fungal peroxidases, known as the aromatic peroxygenases (APO), has been discovered. Members of these extracellular biocatalysts produced by agaric basidiomycetes such as Agrocybe aegerita or Coprinellus radians catalyze reactions—for example, the peroxygenation of naphthalene, toluene, dibenzothiophene, or pyridine—which are actually attributed to cytochrome P450 monooxygenases. Here, for the first time, genetic information is presented on this new group of peroxide-consuming enzymes. The gene of A. aegerita peroxygenase (apo1) was identified on the level of messenger RNA and genomic DNA. The gene sequence was affirmed by peptide sequences obtained through an Edman degradation and de novo peptide sequencing of the purified enzyme. Quantitative real-time reverse transcriptase polymerase chain reaction demonstrated that the course of enzyme activity correlated well with that of mRNA signals for apo1 in A. aegerita. The full-length sequences of A. aegerita peroxygenase as well as a partial sequence of C. radians peroxygenase confirmed the enzymes’ affiliation to the heme-thiolate proteins. The sequences revealed no homology to classic peroxidases, cytochrome P450 enzymes, and only little homology (<30%) to fungal chloroperoxidase produced by the ascomycete Caldariomyces fumago (and this only in the N-terminal part of the protein comprising the heme-binding region and part of the distal heme pocket). This fact reinforces the novelty of APO proteins. On the other hand, homology retrievals in genetic databases resulted in the identification of various APO homologous genes and transcripts, particularly among the agaric fungi, indicating APO’s widespread occurrence in the fungal kingdom. KW - Peroxygenase KW - Chloroperoxidase KW - Cytochrome P450 KW - Heme-thiolate KW - Oxygenation KW - Coprinellus Y1 - 2009 UR - http://link.springer.com/article/10.1007%2Fs00253-009-2000-1 U6 - https://doi.org/10.1007/s00253-009-2000-1 SN - 1432-0614 VL - 84 IS - 5 SP - 885 EP - 897 ER - TY - CHAP A1 - Fricke, Florian A1 - Werner, André A1 - Janßen, Benedikt A1 - Hübner, Michael A1 - Ribbe, Clemens A1 - Cengizhan, Inac T1 - Redesign of an Educational Robot Platform Using Web-based Programming T2 - Proceedings of the 2016 Workshop on Embedded and Cyber-Physical Systems Education, Pittsburgh, PA, USA - October 01-07, 2016 Y1 - 2016 SN - 978-1-4503-4657-3 U6 - https://doi.org/10.1145/3005329.3005332 PB - ACM CY - New York, NY ER - TY - GEN A1 - Weinhardt, Lothar A1 - Fuchs, Oliver A1 - Fischer, André A1 - Weigand, Markus A1 - Meyer, Frank A1 - Benkert, Andreas A1 - Blum, Monika A1 - Bär, Marcus A1 - Pookpanratana, Sujitra A1 - Denlinger, Jonathan D. A1 - Heske, Clemens A1 - Umbach, Eberhard T1 - Site- and Symmetry-resolved Resonant X-ray Emission Study of a Highly-ordered PTCDA Thin Film T2 - The Journal of Physical Chemistry: C Y1 - 2016 U6 - https://doi.org/10.1021/acs.jpcc.5b12422 SN - 1932-7447 SN - 1932-7455 VL - 120 IS - 16 SP - 8607 EP - 8615 ER -