TY - GEN A1 - Michalik, Stephan A1 - Reder, Alexander A1 - Richts, Björn A1 - Faßhauer, Patrick A1 - Mäder, Ulrike A1 - Pedreira, Tiago A1 - Poehlein, Anja A1 - Heel, Auke van A1 - Tilburg, Amanda van A1 - Altenbuchner, Josef A1 - Klewing, Anika A1 - Reuß, Daniel A1 - Daniel, Rolf A1 - Commichau, Fabian M. A1 - Kuipers, Oscar A1 - Hamoen, Leendert A1 - Völker, Uwe A1 - Stülke, Jörg T1 - The Bacillus subtilis Minimal Genome Compendium T2 - ACS Synthetic Biology N2 - To better understand cellular life, it is essential to decipher the contribution of individual components and their interactions. Minimal genomes are an important tool to investigate these interactions. Here, we provide a database of 105 fully annotated genomes of a series of strains with sequential deletion steps of the industrially relevant model bacterium Bacillus subtilis starting with the laboratory wild type strain B. subtilis 168 and ending with B. subtilis PG38, which lacks approximately 40% of the original genome. The annotation is supported by sequencing of key intermediate strains as well as integration of literature knowledge for the annotation of the deletion scars and their potential effects. The strain compendium presented here represents a comprehensive genome library of the entire MiniBacillus project. This resource will facilitate the more effective application of the different strains in basic science as well as in biotechnology. Y1 - 2021 UR - https://pubs.acs.org/doi/10.1021/acssynbio.1c00339 U6 - https://doi.org/10.1021/acssynbio.1c00339 SN - 2161-5063 VL - 10 IS - 10 SP - 2767 EP - 2771 ER -